Browsing by Author "Çobanoğlu, Özden"

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Boz ve yerli kara sığır ırklarında kalpain 1 p.ala316gly genotiplerine ait genetik varyasyonun belirlenmesi
(Bursa Uludağ Üniversitesi, 2022-07-26) Ardıçlı, Sena; Çobanoğlu, Özden; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0003-2758-5945; 0000-0001-9633-634X
Kalpain 1 (CAPN1) geni, mikromolar kalsiyum–aktive nötral proteaz geni olarak bilinir ve postmortem koşullarda miyofibriler proteinleri indirgeyen kalsiyum bağımlı sistein proteazı, μ-kalpaini ayrıştırır. Bu genin kas metabolizması ve gelişimi üzerinde önemli etkileri vardır. Bu gen, çeşitli sığır ırkları arasında geniş çapta çalışılmış olmasına rağmen, Türk yerli sığırları hakkında sınırlı bilgi bulunmaktadır. Bu nedenle bu çalışma, bazı Türk yerli sığır ırklarında CAPN1 p.Ala316Gly polimorfizmine ait genetik varyasyonu belirlemeyi amaçlamıştır. Bu kapsamda 99 Boz ve 41 Yerli Kara ırkı erkek sığır PCR-RFLP metodu kullanılarak genotiplendirilmiştir. Genotipik ve alelik frekanslar, Hardy-Weinberg dengesi (HWE), heterozigotluk (He), polimorfizm bilgi içeriği (PIC), efektif alel sayısı (Ne), fiksasyon indeksi (Fıs), olası varyasyon gerçekleşme düzeyi (%V) dahil olmak üzere popülasyon genetik parametreleri değerlendirilmiştir. Ayrıca Shannon-Weaver indeksi, Simpson dominantlık indeksi ve Gini katsayısını içeren biyoçeşitlilik indeksleri hesaplanmıştır. Sonuçlar, GG genotipinin her iki yerli ırkta da oldukça baskın olduğunu ortaya koydu. Öte yandan, toplam popülasyonda CC genotipinin bulunmadığı gözlenmiştir. Bu durum dikkate değer düzeyde düşük C alel frekansıyla sonuçlanmıştır (Boz ve Yerli Kara için sırasıyla 0.13 ve 0.12). Fisher’ın kesin testi, HWE’den sapma oldğunu, popülasyon genetiği parametreleri ise, incelenen ırklarda oldukça düşük bir genetik varyasyon düzeyi olduğunu göstermiştir. Bu gözlem, düşük seviyedeki biyolojik çeşitlilik seviyeleri ile desteklenmiştir. Nitekim CAPN1 markörü, Boz ve Yerli Kara sığırları için düşük seviyede bilgilendiricilik göstermiştir ancak Türkiye’deki yerli sığır ırklarının genetik karakteri hakkında ileride yapılacak çalışmalara ihtiyaç vardır. Yerli ırklarda yapılacak moleküler genetik çalışmalar, önemli biyolojik süreçlerle ilgili daha geniş perspektifleri ortaya koymak ve büyüme, kas gelişimi ve yemden yararlanma gibi kompleks özelliklerin daha iyi anlaşılmasını sağlamak için teşvik edilmelidir.
Detennination of erythrocyte potassium and glutathione polymorphism in Saanen, Maltese and Turkish hair goats
(Medwell Online, 2011) Gürcan, Eser Kemal; Pala, Akın; Çobanoğlu, Özden; Uludağ Üniversitesi/Veterinerlik Fakültesi/Genetik Bölümü.; 0000-0001-9633-634X; AAM-4544-2020; 9234694200
Main purpose of this study was to investigate genetic polymorphisms of Saanen (S), Maltese (M) and Turkish Hair (TH) goats based on erythrocyte potassium and glutathione types. It was also designed to detect the association between these components and some blood parameters depending on the breed comparisons. The phenotypic and alleles frequencies were calculated for potassium and glutathione types of all three breeds. The difference between low and high potassium (LK and HK, respectively) types for the means of erythrocyte potassium concentration (K-e) were statistically important for all breeds (p<0.01). The significant relationships between erythrocyte potassium types and blood parameters of erythrocyte sodium (Na-e), whole blood potassium (K-wb), plasma potassium (K-p), erythrocyte potassium (K-e) were observed for S and TH goats. On the other hand, the important relationships were detected between erythrocyte potassium types and blood parameters of whole blood sodium (Na-wb), Na-e K-wb, K-p, K-e, total monovalent cation concentration in erythrocyte (Na-e+K-e) for M goat breed (p<0.05). The correlation coefficient between Na-e and K-e blood parameters was important and it was ranged from -0.26 to -0.34. In addition, Maltese goat breeds had a higher K-e than the others but Na-e was higher in TH goats. Even if parity and birth type differences were not statistically important for all blood parameters, there was indication of significant differences for these values except hematocrit value (PCV), Na-p and GSH concentrations depending on the potassium types within breed (p<0.05) and also among all breed analyses (p<0.001).
Determination of body weight-age relationship by non-linear models in Japanese quail
(Medwell Online, 2012) Gürcan, Eser Kemal; Genç, Serdar; Çobanoğlu, Özden; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-9633-634X; AAM-4544-2020; 9234694200
The objectives of this study were to determine the body weight-age with different models and to choose the best fitted model. The study was designed to collect a total of 8 weekly body weight records from 142 quails from birth up to 56 days of age in Tekirdag, Turkey, The Negative Exponential, Brody, Gompertz, Logistic and Bertalanffy Models were used to determine growth characteristics of quail in the present study. The model parameters were calculated for each model and the comparisons among the models were done based on the coefficient of determination (R(2)), adjusted coefficient of determination (R(adj)(2)), Mean Square Error (MSE), the Akaike's Information Criteria (AIC), Residual Standard Deviation (RSD) and Residual Mean (RIVI) as fit criteria. As a conclusion, the Logistic Model (R(2) = 0.98, R(2) = 0.97, MSE = 9.79, AIC = 20.49, RSD = 3.13 and RM = 6.42) resulted in the best fit model for body weight-age in Japanese quail.
Determination of the genetic polymorphism for DGAT1 gene in Holstein, Jersey and native cattle breeds of Turkey
(Agricultural Research Communication Centre, 2019-02-01) Gürcan, E. K.; Çobanoğlu, Özden; Kul, E.; Abacı, H. S.; Çankaya, S.; ÇOBANOĞLU, ÖZDEN; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; AAM-4544-2020
This study was to determine the genetic polymorphism in terms of the DGAT1 gene among Holstein and Jersey and indigenous Native Black, East Anatolian Red and Grey Steppe in Turkey. The frequencies of K allele for the DGAT1 gene were calculated as 0.59, 0.54, 0.53, 0.62, 0.52, and 0.54 for Jersey, Holstein (Black Sea Region), Holstein (Marmara Region), and Grey Steppe, East Anatolian Red, and Native Black breeds, respectively. The F-IS values of the populations for the DGAT1 gene were detected all negative values. In overall population, F-IS value was found as 76%. The expected deviations from the Hardy-Weinberg Equilibrium in terms of the DGAT1 locus were found significant in these six different populations (P<0.01). The genetic distance values among the populations were calculated between 0.0001 and 0.0089. Based on the cluster analysis, Holstein, Native Black and East Anatolian Red were located in close cluster; however, Jersey and Grey Steppe were grouped in different cluster.
Effects of lactation month and season on test-day milk yield and milk components in Holstein cows
(Agricultural Research Communication Centre, 2016-07-28) Gürcan, Eser Kemal; Çankaya, Soner; Kul, Ertuğrul; Abacı, Samet Hasan; Ülker, Mehmet; Çobanoğlu, Özden; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-9633-634X; AAM-4544-2020; 9234694200
This study aims to determine the correlations between the test-day milk yield (TDMY) and the fat and protein content as well as the fat and protein yields in Holstein dairy cows and to investigate the effects of lactation period and sampling season on these parameters. The study material consists of 1.380 records of a total of 151 head of Holstein dairy cows raised on a private farm in Samsun province. In the study, the mean TDMY, fat content, protein content, fat yield and protein yield were recorded as 20.6 +/- 5.10 kg, 3.97 +/- 0.734%, 3.23 +/- 0.276%, 0.81 +/- 0.210 kg and 0.66 +/- 0.160 kg, respectively. The effects of lactation period and sampling season on the TDMY, fat content, protein content, fat yield and protein yield were found statistically significant (P<0.01). Statistically significant negative correlations were determined between the TDMY and the fat and protein content, but positive and statistically significant (P<0.01) correlations between the TDMY and the fat and protein yields. In conclusion, it might be stated that the effects of lactation period and sampling season should also be taken into account to improve milk yield and milk components.
Erythrocyte potassium and glutathione polymorphism determination in Saanen x Malta crossbred goats
(Academic Journals, 2011-07) Gürcan, Eser Kemal; Köse, Merve; Çobanoğlu, Özden; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-9633-634X; AAM-4544-2020; 9234694200
This research is aimed at determining the erythrocyte potassium and glutathione polymorphisms and also to identify the relationship among the various blood parameters in Saanen x Malta crossbred goat raised in Turkey. The allele gene frequencies of K-H and K-L associated with the potassium concentration were calculated as 0.94 and 0.06, respectively. The differences between the mean values of low and high potassium concentrations in erythrocyte were statistically significant (P < 0.01). In addition, there were some significant relationships between erythrocyte potassium types and some blood parameters such as whole blood sodium and potassium concentrations, erythrocyte sodium and potassium concentrations and total monovalent cation concentration in erythrocyte (P < 0.05). The allele gene frequencies of GSH(H) and GSH(h) related with the glutathione concentration were calculated as 0.43 and 0.57, respectively. The difference between the mean values of low and high glutathione erythrocyte concentrations were also statistically significant (P < 0.01). Finally, the significant correlation coefficient between erythrocyte sodium and potassium concentrations was observed in this study (P < 0.05).
Establishment of AA-NAT, SCD, PROP1, and IGFBP3 gene frequency distributions in Karacabey merino and kivircik sheep breeds
(Hellenic Veterinary Medical Soc, 2023-07-01) Dinçel, Deniz; Çobanoğlu, Özden; DİNÇEL, DENİZ; ÇOBANOĞLU, ÖZDEN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı; JKT-4511-2023; ESN-8515-2022
The aim of the current study was to investigate the genotypic dispersion of AA-NAT, SCD, PROP1, and IGFBP3 genes in the target population. Primarily, the genomic DNA was isolated from blood samples by the phenol/chloroform method. The genomic analyses were performed on a total of 160 Karacabey Merino (KM) (n=80) and Kivircik sheep (n=80). The PCR-RFLP method was used to determine the genotype of the SNPs. Deviation from Hardy-Weinberg equilibrium (HWE) and population genetic assays such as observed (Ho) and expected (He) hetero-zygosities, effective allele numbers (Ne), and the polymorphism information content (PIC) were calculated for each gene in the population. As a result, there was no deviation from HWE was determined except SCD and PROP1 gene in KM. The most frequent genotypes were found AA (66,25% and 87,50%) for AA-NAT gene, CC (93,75% and 91,25%) for SCD gene and CC genotype (70,00% and 56,25%) for PROP1 gene in KM and Kivircik sheep. Both breeds were detected monomorphic in terms of the IGFBP3 gene. In conclusion, the other regions of the IGFBP3 gene which were determined as monomorphic in the studied flock could be researched. Moreover, further studies should be necessary for defining the effects of investigated genes on related traits in KM and Kivircik sheep in terms of polymorphic genes specified.
Flexible alternatives to modelswidely used for describing growth in japanese quail
(Pakistan Agricultural Scientists Forum, 2017-02) Gürcan, Eser Kemal; Kaplan, Selçuk; Çobanoğlu, Özden; Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-9633-634X; AAM-4544-2020
Mathematical functions for modeling the growth of animals are separated into two groups called fixed and flexible according to their inflection behaviors. The aims of this study were to compare fixed and flexible growth functions and to determine the best fit model for the growth data of Japanese quail. The long term growth patterns of Japanese quail were monitored during 110 days from day-old to slaughtering, and were analyzed. The fixed and flexible models used in the study were Gompertz, Logistic, Von Bertalanffy and Levakovich, Janoschek, Morgan Mercer Flodin, respectively. The differences in weight between growth profiles of female and male birds were tested via profile analysis also known as Repeated MANOVA. The results of Profile Analysis revealed that females' live weights were heavier (P<0.01). In order to evaluate growth functions, the R-2 (coefficient of determination), adjusted R-2 (adjusted coefficient of determination), MSE (mean square error), AIC (Akaike's information criterion) and BIC (Bayesian information criterion) goodness of fit criteria were used. In the study, it was determined that the best fit model from female and male quail growth data on the basis of these goodness of fit criteria is Janoschek model which has a flexible structure. The Janoschek model is not only important because it has a higher number of parameters with biological meaning than the other functions (the mature weight and initial weight parameters), but also because it was not previously used in the modeling of the quail growth.
Genetic Polymorphism of STAT1 and STAT5A Genes in Holstein, Jersey, and Indigenous Cattle Breeds in Turkey
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020-03-01) Kul, Ertuğrul; Abacı, Samet Hasan; Gürcan, Eser Kemal; Çankaya, Soner; Çobanoğlu, Özden; ÇOBANOĞLU, ÖZDEN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Genetik Anabilim Dalı.; 0000-0001-9633-634X; AAM-4544-2020
This study aimed to determine genetic polymorphism in STAT1 and STAT5A genes for dairy cattle and some native cattle breeds in Turkey. 283 Jersey and a total of 472 Holstein cows from two different herds and 93 Grey Steppe, 85 Anatolian Black Cattle, and 66 East Anatolian Red cattle were used in this research. Generally, C allele gene frequency was higher than T allele for STAT1 in all breeds whereas C allele gene frequency was detected higher than G allele for STAT5A in Jersey and East Anatolian Red. On the other hand, G allele gene frequency was higher than C allele in Holstein, Grey Steppe, and Anatolian Black Cattle breeds. The expected deviations from the Hardy-Weinberg Equilibrium were significant only for Jersey breeds for STAT1 gene. Meanwhile, the expected deviation from equilibrium was also significantly different for Holstein in Black Sea Region (BSR), Anatolian Black Cattle and Grey Steppe for the STAT5A gene. FIS values were determined to STAT1 gene as negative for all breeds except for Holstein in Marmara Region (MR). Similarly, this value was determined to STAT5A gene as positive for all breeds except for Holstein in BSR. The genetic distances for two loci were calculated between 0.0029 and 0.1599 among all populations. Depending on the cluster analysis, Holstein in BSR and MR, Anatolian Black Cattle, East Anatolian Red were closely clustered to each other, while Grey Steppe and Jersey were located in completely different clusters. As a conclusion, based on the detected genetic diversity in STAT1 and STAT5A genes, it is possible to make a genetic improvement among bovine breeds raised in Turkey.
The influence of calving year on milk yield and milk components in dairy cattle
(Bursa Uludağ Üniversitesi, 2018-09-18) Kul, Ertuğrul; Çobanoğlu, Özden; Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0001-9633-634X
The aim of this study was to identify the effect of calving year on milk yield and milk composition traits in Holstein and Jersey cattle raised in the Marmara and Black Sea Regions of Turkey, respectively. The data set consisted of 582 dairy cows, which were 306 Holstein raised and 276 Jersey cows calving from 2011 to 2013. Except for fat yield, test day milk yield (TDMY), 305-day milk yield (305 DMY), fat percentage, protein percentage and protein yield of Jersey cows were affected by calving season. The effect of calving year on TDMY, 305 DMY, fat percentage, fat yield, protein percentage and protein yield of Holstein cows were found to be statically significant. In conclusion, in 2011 to 2013, milk yield and its composition in both Holstein and Jersey cows were significantly related to calving year.
Influence of some environmental factors on milk yield and milk components traits in jersey cows
(Parlar Scientific Publications (P S P), 2019-01-01) Kul, Ertuğrul; Gürcan, Eser Kemal; Çankaya, Soner; Abacı, Samet Hasan; Ülker, Mehmet; Çobanoğlu, Özden; ÇOBANOĞLU, ÖZDEN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0001-9633-634X; AAM-4544-2020
This study was conducted to assess the effects of stage of lactation, parity and sampling season on milk yield and milk components in Jersey cows in Karakoy State Farm, Turkey. The data set consisted of 2657 milk records of 204 Jersey cows collected from September 2011 to December 2013. For this purpose, test day milk yield (TDMY) records and milk samples were taken once a month for 10-month period after parturition. The effect of stage of lactation, parity and sampling season on TDMY, fat percentage, protein percentage, fat yield and protein yield were found statistically significant (P<0.01). TDMY were correlated with fat percentage (-0.235) and protein percentage negatively (-0.254) but with fat yield (0.755) and protein yield positively (0.950) (P<0.01).In conclusion, these results suggest animal breeders that the effects of lactation stage, parity and sampling season may be taken into account in husbandry management to improve the milk yield and milk quality in Jersey cows.
Physiological mechanisms of multiple ovulations and factors affecting twin calving rates in cattle
(Uludağ Üniversitesi, 2011-05-09) Çobanoğlu, Özden; Uludağ Üniversitesi/Veterinerlik Fakültesi.
Reproduction in bovine is affected by several different factors. These factors explain some of the variations observed in the reproductive efficiency of animals. Several comprehensive studies were conducted to assess the incidence of twinning at birth in various dairy and beef cattle breeds. In this manner, many environmental and genetic factors affecting reproductive system have been studied quite extensively. Even if the natural incidence of multiple births in cattle is very low, some variations on the twinning rate can be observed due to the influence of breed differences, the effects of feeding and management systems and also the effects of some environmental sources such as a parity, an age of cow, a season of the year and a geographic location of raised animals. Thus, the main purpose of this review is to emphasize and explain the physiological mechanisms regulating multiple ovulations in cattle and also some of the important factors affecting multiple births positively or negatively on the cow production system.
Twinning in cattle: Desirable or undesirable?
(Uludağ Üniversitesi, 2010) Çobanoğlu, Özden
Twin calving in dairy cow production is an avoidable reproductive event. Twinning may have not only positive but definitely negative effects on cow-calf production system. Because of several detrimental effects related with this incidence, cow producers are mostly against to twin birth in dairy herds. On the other hands, having twin calves may enhance production efficiency if an appropriate nutrition and management system can be operated in beef cattle herds. Therefore, the purpose of this review is to discuss and summarize the effect of twinning on reproduction and production system for dairy and beef industries. Finally, it will give an idea whether twin calving is desirable or undesirable characteristic.