Browsing by Author "İpek, Ahmet"

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Assessment of genetic relationship among male and female fig genotypes using simple sequence repeat (SSR) markers
(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2017-04-05) Tangu, Nesrin Aktepe; Durgut, Erdem; Ercişli, Sezai; Teoman, Sevin; İpek, Bayram; Ertürk, Ümran; Barut, Erdoğan; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0003-1469-6777; AAE-4675-2019; AAH-3233-2021; AAG-7343-2021; AAE-6913-2019; AAH-2551-2021; 57194462289; 16031208900; 7801661220; 26657823900; 6603912487
Fig (Ficus carica L.) is a traditional crop in Turkey and widely cultivated around the Mediterranean areas. The gynodioecious fig species is present in two sexual forms, i.e. the domesticated fig (female tree) and the caprifig (male tree). Caprifigs are crucial for high quality fig production and breeding while, the studies on assessment of genetic relationship among caprifigs is limited. The aim of this study was to determine genetic diversity among 45 caprifigs and 2 female figs collected from four provinces in Marmara and Aegean Sea Regions of Turkey using simple sequence repeat (SSR) markers. In this work, 24 SSR markers were tested, one was monomorphic and the remaining markers amplified 82 alleles. The number of polymorphic alleles per SSR marker ranged from 2 to 7. The observed heterozygosity (Ho) differed from 0.18 to 0.76 and expected heterozygosity (He) ranged between 0.24 and 0.81. The polymorphism information content (PIC) varied from 0.42 to 0.98. A UPGMA analysis based on Dice similarity matrix clustered fig genotypes into two main groups and similarly, STRUCTURE analysis placed fig genotypes into two different gene pools (K=2). Fig genotypes collected from the same region were not clustered together in a group indicating that the fig genotypes did not cluster on the basis of their collection sites. Our results demonstrated that caprifigs and female figs are not genetically distinct and they clustered together in a group. All fig genotypes had distinct SSR marker profiles suggesting that there were no synonyms or homonyms. These results revealed a high genetic variation among fig genotypes and 23 SSR markers were enough to discriminate all fig genotypes analysed in this study demonstrating that SSR marker system is suitable for genetic analysis in figs.
Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers
(Taylor & Francis, 2010-09) Akçay, Mehmet Emin; Gülen, Hatice; İpek, Ahmet; Ergin, Sergül; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-7720-5536; AAG-6558-2020; 6603211102; 6603912487; 39661052000; 6602612385
The characterisation of sweet cherry (Prunus avium L.) genetic resources in Turkey may help to increase their use in breeding programmes worldwide, as Turkey is the centre of origin of sweet cherry. Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were therefore used to analyse genetic diversity among a total of 78 local and introduced sweet cherry cultivars. Four AFLP primer combinations, and six SSR primer pairs for sweet cherry were used for genetic diversity analysis. A genetic similarity matrix was calculated using the combined data from AFLP and SSR analyses with simple matching coefficient. Genetic similarities among the sweet cherry genotypes studied were higher than 42%. No two accessions had an identical AFLP and SSR marker profile, indicating that all 78 genotypes were unique. An UPGMA dendrogram, based on the similarity matrix, revealed 18 separate Groups at or above the 70% similarity level. While some Groups consisted of both introduced and local genotypes, other Groups had only local genotypes. This result suggests that there was broad genetic diversity among the local Turkish sweet cherry genotypes, which was not present in the introduced sweet cherry accessions. The genetic variation present in local Turkish sweet cherry genotypes may be useful for future breeding programmes. We found that the use of both SSR and AFLP marker systems was effective for distinguishing between genetically-close sweet cherry genotypes. These marker systems can be used to complement pomological and morphological markers during the characterisation and identification of sweet cherry genotypes.
Assessment of inter- and intra-cultivar variations in olive using SSR markers
(University of Sao Paolo, 2012) İpek, Ahmet; Barut, Erdoğan; Gülen, Hatice; Ipek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAE-6913-2019; AAH-3233-2021; 6603912487; 26657823900; 6603211102; 16031208900
Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this high level of genetic variation is probably due to the location of Turkey in the center of the origin of olive. The UPGMA dendrogram, developed to visualize the estimated genetic relationships among the 135 samples, demonstrated that the clustering of olive cultivars was not based on geographical regions of cultivation. Presence of genetic variation was detected within a nationwide grown Turkish olive cultivar, called 'Gemlik'. Olive growers successfully discriminated olive cultivars with distinct morphological and pomological characters. However, there was some confusion about the identification of cultivars with similar phenotypic traits. To prevent misidentification of olive cultivars and to minimize intra-cultivar variation, certified propagation materials which were characterized using DNA based molecular markers should be used during the establishment of new olive orchards.
Association of reversible inactivation of the maize transposable element Ds with tissue-specific processing of the 35S : TPase transcript in carrot (Daucus carota L.)
(Taylor & Francis, 2006-09) Simon, Philipp W.; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Ziraat Fakültesi Bahçe Bitkileri Bölümü.; AAH-3233-2021; 6603912485
An Ac/Ds-based two-element transposon tagging system has been introduced into carrot. F-1 progeny containing both the 35S-Ac-transposase gene (35S:TPase) and the Ds element were derived from crosses between 35S:TPase- and Ds-bearing parents. While excision of Ds was not detected in any F-1 plants carrying both 35S:TPase and the Ds element, calli initiated from these F-1 plants had the Ds element excised, indicating Ds transposition. Reverse transcriptase-PCR analysis revealed that the 35S:TPase gene was expressed in both F-1 plants and calli, and that introns 1, 2, and 3 were spliced correctly. Although intron 4 was also spliced correctly in calli, incorrectly spliced intron 4 was detected in F-1 plants. Sequence analysis of incorrectly spliced reverse transcriptase-PCR products demonstrated the presence of a cryptic intron donor site within intron 4 of the 35S:TPase transcript. This probably competed with the proposed intron donor site during maturation of the major 35S:TPase transcript. These results suggested that the major transcript of 35S:TPase was incorrectly processed and, consequently, that the Ds element was reversibly inactivated in the somatic tissues of carrot plants, whereas this inactive Ds element was remobilised during tissue culture, where the 35S:TPase transcript was spliced correctly. These observations point to an important role for tissue-specific 35S:TPase transcript processing for successful transposition of Ds in carrot. Therefore, successful processing of the 35S:TPase transcript in carrot callus may indicate strategies to increase Ac transposition in other tissues.
Biberde TSWV dayanımının yerli kapya biber çeşitlerine moleküler işaretleyiciler kullanılarak aktarılması
(Bursa Uludağ Üniversitesi, 2021-02-16) Çoban, Onur; İpek, Ahmet; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Anabilim Dalı.; 0000-0003-3282-5325
Ülkemizde yoğun bir şekilde üretimi ve tüketimi yapılan biber tiplerinin başında kapya biberi gelmektedir. Taze tüketimin yanı sıra, sanayi alanında da çok fazla kullanım alanları bulunmaktadır. Son dönemlerde kapya biber üretimini etkileyen faktörlerin başında tomato spotted wilt virüs (TSWV) hastalığı gelmektedir. TSWV hastalığının kimyasal mücadelesi bulunmamasından dolayı, üretimlerde verim kayıplarına sebep olarak, tarımda büyük ekonomik kayıplara neden olmaktadır. TSWV hastalığı ile en etkin mücadele yöntemlerinin başında, bu hastalığa dayanıklı çeşitlerin kullanılması gelmektedir. Bu çalışmada, yerel kapya biber çeşidine (Ata-16) TSWV ye dayanıklı olduğu bilinen ticari olarak satışı yapılan kapya biber çeşidinden (Nr-07), moleküler markör yöntemleri kullanılarak TSWV dayanıklılık geninin aktarılmasını sağlayarak, üretimde hem ekonomik kayıpları en aza indirmek, hem de yerel kapya biber çeşidimizin yok olmasının önüne geçilmesi hedeflenmiştir. Buna ek olarak, bu tez sonucunda elde edilen bitkisel materyaller (TSWV) hastalığına dayanıklı yeni kapya biber çeşitlerinin geliştirilmesi için gen kaynağı olabilecektir.
Characterization of an unusual cytoplasmic chimera detected in bolting garlic clones
(American Society of Horticultural Science, 2007-07-02) İpek, Meryem; Senalik, Douglas; Simon, Philipp W.; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 6603912487
Production of a visible flower stalk, or bolting, has been used as a major trait to categorize garlic (Allium sativum L.) clones. Analysis of mitochondrial genome variation with polymerase chain reaction (PCR) revealed differences between bolting and nonbolting clones of garlic. Screening 333 garlic accessions from diverse geographic origins revealed a 1403-bp mitochondrial DNA marker associated with bolting that the authors call "Bolt Marker" (BltM). Bolt Marker did not amplify in any of the 131 nonbolting clones, whereas amplification of this marker was observed in 127 of 130 (97.7%) garlic clones that bolted completely in Wisconsin. Seventy-two garlic clones bolted incompletely (clones in which some but not all of the plants bolted), and this marker was not amplified in 69 (95.8%) of these clones. Because of the significant association of BltM with bolting, this PCR-based marker can be used to discriminate complete-bolting garlic clones reliably from nonbolting and incomplete-bolting ones. Sequence characterization of this marker revealed that BltM is a chimera involving both mitochondrial and chloroplast DNA. The DNA sequences including and flanking both the 5' and 3' ends of this marker are consistent with an approximate to 4.8-kbp chloroplast DNA fragment having been inserted into the mitochondrial genome downstream from the mitochondrial cox3 gene. Sequence alignment of the chloroplast genes in this chimeric region with the homologous sequences in GenBank indicate the presence of deletions, insertions, and single nucleotide polymorphisms in the coding sequences, resulting in putative, incomplete open reading frames or frame shift mutations. Hence, the authors speculate that this insertion may have occurred long ago in the evolution of garlic.
Determination of self-incompatibility groups of sweet cherry genotypes from Turkey.
(Funpec-Editora, 2011) Akçay, Mehmet Emin; İpek, Ahmet; Gülen, Hatice; İpek, Meryem S.; Ergin, Sergül; Eriş, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-7720-5536; AAG-6558-2020; AAH-3233-2021; 6603912487; 6603211102; 16031208900; 39661052000; 6602612385
Determination of S-allele combinations of sweet cherry genotypes and cultivars has importance for both growers and breeders. We determined S-allele combinations of 40 local Turkish sweet cherry genotypes using a PCR-based method. Ten different S-alleles were detected. Although the most common S-allele was S-3, as also found in Western genotypes and cultivars, there were some differences in the frequencies of some S-alleles between Turkish and Western sweet cherry genotypes. According to their S-allele compositions, 30 local Turkish sweet cherry genotypes were assigned to 10 previously identified incompatibility groups. For the remaining genotypes, whose S-allele combinations did not fit to any previous incompatibility groups, three more incompatibility groups, XLII, XLIII and XLIV, were proposed. Results obtained from this study will help both sweet cherry growers and breeders to better manage these local Turkish sweet cherry genotypes in their orchards.
Development of aflp markers associated with zucchini yellow mosaic virus resistance in cucumber (Cucumis sativus L.)
(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2015-01-01) Sigva, Hasan Ozgur; Fırat, Ahmet Fikret; Hazarhun, Gulden; İpek, Ahmet; İPEK, AHMET; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü; 0000-0001-5821-2426; AAK-4655-2021
Zucchini yellow mosaic virus (ZYMV) is one of the most important pathogens that cause significant yield losses in many cucurbit crops including cucumber (Cucumis sativus L). ZYMV resistance in cucumber is inherited by a single recessive gene. The purpose of this study was to identify molecular markers linked to the gene conferring ZYMV resistance in cucumber. We developed a population of 188 F-2 plants derived from inbred cucumber lines. Individual F-2 plants were self-pollinated to generate F-3 populations. Ten randomly selected plants from each F-3 population were tested for ZYMV resistance. We used a bulk segregant analysis method to identify putative molecular markers linked to ZYMV resistance. Using bulked DNA samples with parental lines and F-1, a total of 170 sequence-related amplified polymorphism (SRAP), 586 simple sequence repeat (SSR), and 308 amplified fragment length polymorphism (AFLP) primer combinations were screened. Neither polymorphic SRAP nor SSR markers were linked with ZYMV resistance. Among the 308 AFLP primer combinations tested, an AFLP marker in the E-ACA/MCA primer combination showed significant association among parental lines, F-1, and resistant and susceptible plants. The combination of E-ACA/M-CA was achieved on parental lines, F-1, and 188 F-2 individuals for confirmation of the marker segregation on the F-2 population. We found that the combination of E-ACA/M-CA was linked to the zym locus with 6.91 cM.
Development of EST based SSR markers for garlic genome
(Int Soc Horticultural Science, 2012) Simon, Philipp W.; Wako, T.; İpek, Meryem; İpek, Ahmet; Cansev, Asuman; Şeniz, Vedat; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-3233-2021; AAH-4255-2019; 16031208900; 6603912487; 26326677200; 13604787100
Although it is time consuming and expensive to develop SSR markers, they have some advantages such as co-dominancy, reproducibility and high amount of polymorphic alleles as a PCR based marker system. For genetic and molecular studies in garlic, generally RAPD and AFLP markers have been utilized. However, development and use of SSR markers have been limited to few studies. In order to develop detailed genetic map and genetic studies, co-dominant marker systems like SSRs and SNPs are necessary in garlic. The purpose of this study was the development of SSR markers from expressed sequence tags (ESTs) derived from the genome of garlic. The SSR motifs in EST sequences were screened and it was revealed that SSR motifs are abundant in garlic ESTs. So far six SSR markers have been developed. EST based SSR markers could be used to map genes to garlic genetic maps directly and assessment of genetic diversity for the expressed regions of the garlic genome.
Differentially expressed genes in leaf, meristematic and flower tissues of garlic
(Int Soc Horticultural Science, 2012) Senalik, Douglas A.; Simon, Philipp W.; Wako, T.; İpek, Meryem S.; İpek, Ahmet; Aydoğan, Çiğdem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0003-4884-5304; AAH-3233-2021; AAG-6532-2020; 16031208900; 6603912487; 55481474200
Differential gene expression analysis has been investigated to reveal important genes in many plant species. Identification of genes controlling metabolic pathways in garlic could contribute to the understanding of garlic genetic. In this study, transcript (mRNA) profile of differentially expressed genes was determined in leaf, meristematic and flower tissues of three genetically divers garlic clones at different developmental stages using cDNA-AFLP approach. In total, 352 differentially expressed transcript-derived fragments (TDFs) were evaluated from 20 primer combinations. While seven TDFs fragments expressed only in meristematic tissues, 30 TDFs expressed only in leaf tissues. Twenty seven fragments expressed in both meristematic and leaf tissues with intensity polymorphism and two fragments differentially expressed in flower tissues. BLAST analysis of these tissue specific expressed fragments revealed that some genes have significant similarities with previously determined sequences in the database of NCBI GenBank while some of them are unique to garlic genome. These tissue specific expressed genes can be used to develop tissue specific markers for garlic genome.
Distribution of olive (Olea europaea L.) genotypes in the Southern Marmara region of Turkey
(Pakistan Botanical, 2009-06) Barut, Erdoğan; İpek, Ahmet; Gülen, Hatice; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAE-6913-2019; 26657823900; 6603912487; 6603211102
High quality olive oil can be produced from a standard cultivar with high olive oil quality. In this study, olive genotypes grown in the Southern Marmara Region has been determined using simple sequence repeats (SSR) markers. A total of 70 samples from 10 major olive growing locations in the Southern Marmara Region were collected. SSR analysis demonstrated that olive production in this region has been made using primarily a single genotype called "Gemlik". There were some genotypes (8%) were misidentified as "Gemlik" by the farmers since their SSR marker profiles were different from remaining "Gemlik" cultivars. Due to the repropagation of "Gemlik" using green cuttings, it has become the major olive cultivar grown in this region. On the other hand, traditional repropagation of olive using grafted seedlings increases time, expenses and technical expertise required for reproduction. Therefore, repropagation of "Gemlik" using green cuttings promoted the distribution this genotype in the Southern Marmara Region. Although "Gemlik" cultivar is popular and primarily consumed as table olive in Turkey, a standard olive oil production can be made using "Gemlik" cultivar grown in this region. Techniques to increase the quality of olive oil produced from "Gemlik" cultivar should be developed in the future.
Effects of antioxidant enzymes on heat stress tolerance of pepper (Capsicum annuum L.) seedlings
(International Society for Horticultural Science, 2016) Turhan, Ece; Onus, A. N.; Gülen, Hatice; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0001-7586-3108; AFW-5375-2022; AAK-4655-2021; 6603211102; 6603912487
Pepper seedlings (Capsicum annuum L. 'Amazon' and 'Kekova') were grown for 4 weeks at 25/10 degrees C day/night temperatures in a greenhouse and watered on a needs basis avoiding any additional stress factors. Gradual and shock heat stress (GHS and SHS) were applied (from 35 up to 50 degrees C) to the plant in a growth chamber and then heat stress tolerance (HST) was estimated. During the heat treatments, the activities of ROS producing enzyme, NADPH oxidase and ROS scavenging enzymes, catalase (CAT) and ascorbate peroxidase (APX) were measured besides leaf relative water content (RWC) and loss of turgidity. Leaf RWC decreased gradually from control to the highest temperature, while loss of turgidity increased in both heat stress types. Under GHS, HST (LT50) was calculated as 47.1 degrees C for Amazon and 45.6 degrees C for 'Kekova'. On the other hand with SHS, HST for 'Amazon' and 'Kekova' were determined as 47.4 and 42.8 degrees C, respectively. The results clearly show that the cultivar 'Amazon' is superior with respect to its antioxidant defence systems and should be more tolerant than 'Kekova' due to higher ROS-scavenging systems.
Effects of different temperatures on some biochemical contents in garlic bulbs
(American Society for Horticultural Science, 2016-09) İpek, Meryem; Cansev, Asuman; İpek, Ahmet; Koccat, Saime; Cansev, Mehmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; AAH-3233-2021; AAK-4655-2021; FEB-0069-2022; M-9071-2019
Effects of high temperature stress on enzymatic and nonenzymatic antioxidants and proteins in strawberry plants
(TÜBİTAK, 2016-12-03) Ergin, Sergül; Gülen, Hatice; Kesici, Müge; Turhan, Ece; Köksal, Nezihe; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 6603912487
The mechanism of tolerance to high temperatures was investigated in two strawberry (Fragaria x ananassa Duch) cultivars, 'Redlands Hope' ('R. Hope', heat tolerant) and 'Cal. Giant 3' ('CG3', heat sensitive). Leaves were collected from plants that were exposed to gradual heat stress and heat-shock stress separately. The contents of nonenzymatic antioxidants such as ascorbic acid (AsA) and glutathione (GSH) and the activities of enzymatic antioxidants such as ascorbate peroxidase (APX) (EC 1.11.1.11), catalase (CAT) (EC 1.11.1.6), and glutathione reductase (GR) (EC. 1.6.4.2) were measured followed by heat treatments. Additionally, proline content was determined, and heat shock proteins (HSPs) were analyzed with an immunoblotting method to investigate protein markers involved in the heat-stress tolerance of strawberry plants. The contents of AsA and GSH did not change depending on heat stress type, temperatures, or cultivars. While APX and CAT activities increased with high temperatures, GR activity was almost unchanged. The proline content of the cultivars increased in both treatments. Anti-HSP60 immunoblots revealed that a 23 kDa polypeptide was detected during the heat acclimation of strawberry cultivars. The intensity of the heat shock protein in 'R. Hope' plants was more than in 'CG3' plants. Thus, the accumulation of 23 kDa heat shock protein was correlated with the heat tolerance of the cultivars. In conclusion, strawberry leaf tissues of 'R. Hope' were found to enhance the structural stability of cellular membranes under high temperature by increasing both the activity of such enzymes as CAT and APX to activate the antioxidative systems and the expression of 23 kDa HSP.
Genetic characterization of allium tuncelianum: An endemic edible allium species with garlic odor
(Elsevier, 2008-02-21) Simon, Philipp; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 16031208900; 6603912487
Allium tuncelianum (Kollman) Ozhatay, Matthew & Siraneci is a native species to the Eastern Anatolia. Its plant architecture resembles garlic (Allium sativum L.) and it has mild garlic odor and flavor. Because of these similarities between two species, A. tuncelianum has been locally called "garlic". In addition, both A. tuncelianum and garlic has 16 chromosomes in their diploid genomes. Recently, A. tuncelianum has been suggested as the wild progenitor species of garlic. In this study, amplified fragment length polymorphisms (AFLP) markers and nucleotide sequence analysis of the internal transcribed spacer region (ITS) were used to assess genetic and phylogenetic relationships among A. tuncelianum, garlic and some other Allium species. AFLP analysis demonstrated that A. tuncelianum and garlic are genetically distinct and they are likely different species. Phylogenetic analyses based on the nucleotide sequence of ITS suggested that A. tuncelianum and garlic are distinct species and placed A. tuncelianum, garlic, Allium ampeloprasum and Allium scorodoprasum into the same clade in the neighbor joining dendrogram and in the consensus tree of parsimony analysis. However, A. tuncelianum was phylogenetically less related to garlic than either A. ampeloprasum or A. scorodoprasum, suggesting that A. tuncelianum may not be the immediate wild ancestor species of garlic. Further studies to generate hybrid progeny between A. tuncelianum and garlic (if possible) could provide more information on the homology between the chromosomes of A. tuncelianum and garlic and genetic relationships between these two species.
Genetic diversity among some blackberry cultivars and their relationship with Boysenberry assessed by AFLP Markers
(Academic Journals, 2009-10-05) İpek, Ahmet; Barut, Erdoğan; Gülen, Hatice; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; AAE-6913-2019; 6603912487; 26657823900; 6603211102; 16031208900
Blackberry cultivation has increased its popularity in Turkey due to the use of more blackberries in Turkish cuisine. To provide farmers with well adapted blackberry cultivars, some blackberry cultivars including a Boysenberry genotype from North America has been planted to various geographical regions in Turkey. In this study, genetic diversity among these blackberry cultivars and their genetic relationship with Boysenberry and raspberry were analyzed using AFLP markers. Our results indicated that Blackberry cultivars from North America had narrow genetic background which can pose a problem for future breeding programs. Blackberry genotypes selected from Bursa province of Turkey shared all AFLP markers with the cultivar Chester, which suggests that they were not unique genotypes. Although genetic similarity between Boysenberry and blackberry was low, Boysenberry was genetically related to common blackberry cultivars. On the other hand, AFLP analysis was unable to detect any genetic relationship between Boysenberry and common raspberry cultivars from North America in this study.
Genetic diversity among some currants (Ribes spp.) cultivars as assessed by AFLP markers
(Pakistan Botanical, 2010-04) İpek, Ahmet; Barut, Erdoğan; Gülen, Hatice; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAE-6913-2019; AAH-3233-2021; 6603912487; 26657823900; 6603211102; 16031208900
Currants cultivation has increased its popularity in Turkey due to the use of more currants in Turkish cuisine. To provide farmers with well adapted currants cultivars, some currants cultivars have been planted in various geographical regions of Turkey. In this study, genetic diversity among some of these currants cultivars has been analyzed using AFLP markers. Our results indicated that red and black currants genotypes are genetically distinct, sharing very small proportion of AFLP markers. Selected currants genotypes from Turkey shared all AFLP markers suggesting that they might be the same genotype.
Genetic diversity assessment of garlic clones with SSR markers
(Amerikan Society Horticultural Science, 2016-09) Simon, Philipp; İpek, Ahmet; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi.; AAK-4655-2021; AAH-3233-2021
Genetic diversity assessment of garlic clones with SSR markers
(Amer Soc, 2016-09) Simon, Philipp; İpek, Ahmet; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; AAK-4655-2021
Genetic relationships among olive (Olea europaea L.) cultivars native to Croatia and Turkey
(Druckerei Liddy Halm, 2012) Ercişli, Sezai; Bencic, Dani; Liber, Zlatko; İpek, Ahmet; Barut, Erdoǧan; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAE-6913-2019; 6603912487; 26657823900
The aim of the study is to determine genetic diversity and relationships among olive cultivars native to Croatia and Turkey. A total of twenty olive (Olea europaea L.) cultivars including fourteen from Croatia and six common cultivars from Turkey were analyzed for genetic diversity and relationships by using six microsatellite markers (DCA05, DCA09, DCA18, GAPU71B, GAPU101, UDO43). The number of polymorphic alleles ranged from 2 (UDO43) to 5 (DCA09), with an average of 3.6 fragments per marker. UPGMA cluster analysis based on simple matching similarity matrix grouped cultivars into three main clusters. Two pairs of cultivars from Croatia ("Buza muska" and "Levantinka"; "VLMD6" and "Drobnica") were thought to be different, although they produced identical SSR profiles. Cluster analysis points to some genetic relationships between Croatian and Turkish olive cultivars. The results also indicate efficiency of SSR markers to evaluate genetic diversity in olive and identify misnamed or synonym individuals.