Browsing by Author "Eris, Atilla"

Now showing 1 - 9 of 9

Assessment of genetic relationships among 29 introduced and 49 local sweet cherry accessions in Turkey using AFLP and SSR markers
(Taylor & Francis, 2010-09) Akçay, Mehmet Emin; Gülen, Hatice; İpek, Ahmet; Ergin, Sergül; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-7720-5536; AAG-6558-2020; 6603211102; 6603912487; 39661052000; 6602612385
The characterisation of sweet cherry (Prunus avium L.) genetic resources in Turkey may help to increase their use in breeding programmes worldwide, as Turkey is the centre of origin of sweet cherry. Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers were therefore used to analyse genetic diversity among a total of 78 local and introduced sweet cherry cultivars. Four AFLP primer combinations, and six SSR primer pairs for sweet cherry were used for genetic diversity analysis. A genetic similarity matrix was calculated using the combined data from AFLP and SSR analyses with simple matching coefficient. Genetic similarities among the sweet cherry genotypes studied were higher than 42%. No two accessions had an identical AFLP and SSR marker profile, indicating that all 78 genotypes were unique. An UPGMA dendrogram, based on the similarity matrix, revealed 18 separate Groups at or above the 70% similarity level. While some Groups consisted of both introduced and local genotypes, other Groups had only local genotypes. This result suggests that there was broad genetic diversity among the local Turkish sweet cherry genotypes, which was not present in the introduced sweet cherry accessions. The genetic variation present in local Turkish sweet cherry genotypes may be useful for future breeding programmes. We found that the use of both SSR and AFLP marker systems was effective for distinguishing between genetically-close sweet cherry genotypes. These marker systems can be used to complement pomological and morphological markers during the characterisation and identification of sweet cherry genotypes.
Cold hardiness of olive (Olea Europaea L.) cultivars in cold-acclimated and non-acclimated stages: Seasonal alteration of soluble sugars and phospholipids
(Cambridge Univ, 2009-08) Gülen, Hatice; Cansev, Asuman; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; 6603211102; 26326677200; 6602612385
In many plant species, several physiological and biochemical changes occur during low-temperature-induced cold acclimation. A previous study with olive cultivars (Cansev et al. 2009) demonstrated a correlation between the level of accumulation of certain leaf proteins besides antioxidative enzyme activities and cold hardiness of the cultivars. The present paper analysed soluble sugar (SS) and phospholipid (PL) contents of cold-acclimated (CA) and non-acclimated (NA) leaf tissues in order to explain the mechanism of cultivar-dependent response to cold in olive. In general, cold acclimation significantly increased total soluble Sugar (TSS), reducing sugars and sucrose contents of all cultivars to various extents depending on the cold hardiness of cultivars. In addition, TSS, reducing sugars and sucrose contents in cold-tolerant cultivars were significantly increased, whereas TSS, reducing sugars and sucrose contents in cold-sensitive cultivars either did not change or increased slightly in CA stage compared with those in NA stage. Even though reducing sugars were the major soluble sugar in olive leaves, levels of sucrose accumulations in CA stage compared with those in NA stage were greater than those observed in reducing sugars accumulation. Changes in levels of total PL, as well as the three individual PL fractions phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), were investigated in olive leaf tissues. Significant increases in levels of PC and PE fractions during CA compared with those in NA Stage Suggested that PC and PE maintained the cold hardiness of olive cultivars more effectively than did PI. Although the precise mechanisms by which olive responds to cold may still be open to discussion', soluble sugars and PL are clearly important in the ability of olive cultivars to stand against cold stress.
Cold-hardiness of olive (Olea europaea L.) cultivars in cold-acclimated and non-acclimated stages: Seasonal alteration of antioxidative enzymes and dehydrin-like proteins
(Cambridge Univ, 2009-02) Cansev, Asuman; Gülen, Hatice; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; 26326677200; 6603211102; 6602612385
Seasonal patterns of antioxidative enzymes and proteins and their relations to cold-hardiness of nine olive (Olea europaea L.) cultivars (Ascolona, Domat, Gemlik, Hojoblanca, Lecquest, Manzanilla, Meski, Samanli and Uslu) are documented in the current study. Fully expanded, uniformly sized leaves from 2-year-old Shoots Of the cultivars were collected from 20-year-old trees in cold-acclimated (CA, in January) and non-acclimated (NA, in July) stages. Leaf samples were exposed to low temperature at 4, -5, -10 and -20 C for 12 It to determine their cold-hardiness (LT50; assessed by electrolyte leakage). Cold-acclimation produced an increase in freezing tolerance of all cultivars (by lowering LT50). Domat and Lecquest were found to have the highest cold-hardiness among the nine cultivars investigated. Ascolona, Gemlik, Hojoblanca had moderate cold-hardiness, while Samanli, Meski, Uslu and Manzanilla were more sensitive. Activities of catalase (CAT: EC 1.11.1.6), ascorbate peroxidase (APX: EC 1.11.1.11) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase significantly varied depending on the cold-acclimation stage and the cold-hardiness level of the cultivars. Activities of the three antioxidative enzymes and total soluble proteins (TSP) were higher in the CA stage than in the NA stage. Although no accumulation or major polypeptides, except a 23 kDa protein, was detected either in CA samples or NA samples by SDS-PAGE, anti-dehydrin immunoblots revealed that the 43 and 23 kDa polypeptides were detectable during cold-acclimation of olive cultivars, Accumulation of both 43 and 23 kDa dehydrin was significantly higher in the CA stage than in the NA stage in all cultivars. Accumulation of 43 kDa dehydrin was correlated with cold-hardiness of the cultivars, while 23 kDa dehydrin was considered as cultivar-dependent since its accumulation was not parallel to LT50 values of the cultivars. Indeed, the tissues of cvs Domat, Lecquest, Ascolona, Hojoblanca and Gemlik were found to enhance the structural stability Of Cellular membranes in the CA stage by increasing both the activity Of Such enzymes as CAT, APX and NADPII oxidase to activate the antioxidative systems and the expression of 43 kDa dehydrins.
Dormancy in tulip (Tulipa gesneriana L.) bulbs and freesia (Freesia refracta Klatt.) corms: Changes in soluble proteins and APX activity
(Wfl Publ, 2011-04) Köksal, Nezihe; Gülen, Hatice; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 54380475800; 6603211102; 6602612385
The bulbs of tulip (Tulipa gesneriana L.) evs Negrita and Cassini and the corms of freesia (Freesia refracta Klatt.) cvs Polaris and Aladin were used in the study to investigate molecular changes during the storage and sprouting stage besides phenologic observations. One part of the bulbs and corms were directly planted (DP) into greenhouse without any treatment. Another part of the bulbs and corms were either stored in room condition (RC; 18-25 degrees C and 55-65% RH) or in normal atmosphere (NA; 5+/-1 degrees C and 70-85% RH) for 120 days before planting. In both storage treatments, the bulbs and corms were transplanted to the greenhouse in three periods with 40 days intervals. In every period, a part of the bulbs and corms were taken for the molecular analyses before planting. In addition, some of DP bulbs and corms were removed from soil at the same periods for molecular analyses. The storage of the bulbs and corms reduced the sprouting durations in comparison to the DP treatment. However, NA storage either reduced (in tulip) or inhibit (in freesia) to proportions of plant formation. The highest and the lowest ascorbate peroxidase (APX) activity were found during NA storage and DP treatment, respectively, in both species. In addition APX activity was higher in tulip than in freesia. Sharp decrease in total soluble protein (TSP) content particularly after 40 and 60 days of almost all treatments can be an evidence of breakdown of storage proteins. TSP profiles of tulip bulbs showed a major polypeptide, 18 kDa, with different band intensities according to the treatments. In freesia, 66 kDa protein accumulated especially in last two sampling periods of NA storage, whereas 58 kDa protein became invisible in last two sampling periods of NA storage. Since prolonged NA storage inhibits the sprouting and proportions of plant formation, 66 kDa and 58 kDa bands might be related to dormancy or sprouting in freesia.
The effect of harvest maturity stage on ACC synthase activity and total proteins profile in kiwifruits during normal and controlled atmosphere storages
(Friends Science, 2010-11) Öz, Ayşe Tülin; Gülen, Hatice; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 6603211102; 6602612385
This study indicated that the harvest periods related to soluble solids contents (SSC) content of Hayward kiwifruit significantly affected 1-aminocyclopropane-1-carboxylate (ACC) synthase activity, total soluble protein (TSP) content and protein profile ACC synthase activity was suppressed, especially in early harvested fruits, by an inhibition of fruit ripening during controlled atmosphere (CA) storage Separation of TSP using denatured polyacrylamide gel electrophoresis (SDS-PAGE) revealed that one predominant polypeptide with 38 kDa molecular mass that varied among four different harvest periods during normal atmosphere (NA) storage This polypeptide band was accumulated with Increased harvest maturity during NA storage On the other hand, it was not detected on SDS-PAGE of CA samples Consequently CA storage suppressed expression of the 38 kDa protein band, which may be responsible for ACC synthesis SSC of kiwifruits at harvest is an important factor that effects the molecular changes related to ripening of fruits during storage
Effect of low-dose gamma irradiation on the quality of sweet cherry during storage
(Codon Publication, 2008) Akbudak, Bulent; Tezcan, Himmet; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü.; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 56253171700; 7003481568; 6602612385
The efficacy of postharvest gamma irradiation treatment on sweet cherry cv. "0900 Ziraat" was tested under controlled atmosphere. Cherries were stored at six different atmosphere combinations for up to 60 days after being exposed to gamma irradiation. Weight loss values were higher in cherries stored under normal atmosphere compared to controlled atmosphere. Initial firmness was 14.21 and 15.97 N in non-irradiated and irradiated fruits, respectively. While the final values were 2.94 N in normal atmosphere and 5.49 N in normal atmosphere+gamma irradiation. The highest acidity and ascorbic acid values were recorded in the fruit stored under controlled atmosphere (20:5, 25:5)+gamma irradiation. Irradiation reduced spoilage from 25.06 to 5.00%. The fungi most frequently isolated from sweet cherries were Botrytis cinerea, Penicillium expansum, Monilinia fructicola, Alternaria alternata and Rhizopus stolonifer. Better fruit quality was obtained when controlled atmosphere was combined with gamma irradiation compared with either normal atmosphere or controlled atmosphere. Sweet cherry cv. "0900 Ziraat" can be stored successfully for more than 60 days under controlled atmosphere (20:5)+gamma irradiation and partially controlled atmosphere (25:5)+gamma irradiation conditions.
The effects of seaweed (Ascophyllum nodosum) extract on yield and quality criteria in peppers
(International Society Horticultural Science, 1995) Eris, Atilla; Sivritepe, H. Ozkan; Sivritepe, Nuray; Bursa Uludağ Üniversitesi/Ziraat Fakültesi.; 0000-0002-0093-9785; AAH-3249-2020
Three different treatments of seaweed extract (Ascophyllum nodosum) were applied at five different stages of growth, as foliar applications, to examine their effects on yield and quality criteria in peppers (cv. California Wonder) Each treatment caused an increase in fruit yield, and a 10-day earlier first harvest was observed in treatment groups (trt-1, trt-2 and trt-3) compared with control. Fruit length and fruit diameter significantly increased with increased seaweed extract treatment. Nevertheless, the difference between treatments was not significant in wall thickness of fruits. Soluble solids and chlorophyll contents increased whereas titratable acidity decreased in line with the seaweed extract treatments. However, there was no significant difference between control and the seaweed extract treated groups in terms of pH of the fruits. Lt was concluded that trt-2 (340g/da) gave the best results with regard to fruit yield and quality.
Influence of harvest time maturity and storage condition on internal ethylene production rate of 'Hayward' kiwifruits
(Wfl Publication, 2010) Öz, Ayşe Tülin; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 6602612385
Harvesting at the proper maturity stages is essential for optimum quality and often for maintenance of this quality after harvest and storage. The characteristic of commercial harvest maturity of kiwifruit is determined on the basis of total solids (TS) or soluble solids content (SSC). Moreover SSC of kiwifruits at harvest is one of the most important factors that the effects the ethylene production rate related to ripening of fruits during storage. The aim of this study was investigated to the effects of harvest maturity stage of 'Hayward' kiwifruit and storage condition both in normal atmosphere (NA) and controlled atmosphere (CA) on internal ethylene production rate of 'Hayward' kiwifruit (Actinidia deliciosa) changes were studied during storage at 0 degrees and post-storage at 20 degrees C. 'Hayward' kiwifruits harvested periodically according to their soluble solid contents at 4.5-5.5%, 5.6-6.5% and 6.6-7.5% 'Hayward' kiwifruits were stored 5 months at 0 degrees C and 85-90% RH under NA and CA storage. After CA and NA storage fruit held in air at 20 degrees C for 7 days shelf-life. CA atmosphere storage suppressed of fruit ethylene production at 0 degrees C cold storage and post-storage at 20 degrees C. The results from this study indicated that the harvest date related to SSC content of 'Hayward' kiwifruit significantly affected internal ethylene production. Internal ethylene production indicated a significantly difference among the harvest periods during NA storage. Storage conditions were the main factors affecting internal ethylene production rate
Total soluble sugars in tulip bulbs and freesia corms during storage
(INT SOC Horticultural Science, 2010) Erkan, M.; Aksoy, U.; Köksal, Nezihe; Gülen, Hatice; Eris, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 54380475800; 6603211102; 6602612385
In general, bulbs and corms of ornamental plants are highly recommended to store in ambient temperature for certain periods of time depending on the production strategies. During the storage period, many physiological changes occur in bulbs and corms. In this study, total soluble sugars of tulip bulbs (Tulipa gesneriana L. 'Negrita' and 'Cassini') and freesia corms (Freesia reflecta Klatt. 'Polaris' and 'Aladin') were investigated during different storage conditions. The bulbs and corms were stored in either non-cold (18-25 degrees C and 55-65% RH) or cold (5 +/- 1 degrees C and 70-85% RH) storage conditions. Total soluble sugars and loss of weight in bulbs and corms stored in both conditions were evaluated for 120 days with 40 days intervals. Loss of weight in bulbs and corms increased linearly in both storage conditions. In general, total soluble sugar contents were higher in tulip than in freesia. In addition, significant varietal differences were detected between the cultivars. In tulip cultivars, total soluble sugar contents were higher in cold-stored bulbs than that in non-cold stored ones, whereas no significant difference between storage conditions was detected in corms of freesia cultivars. The results indicated significant differences between species as well as cultivars in response to storage conditions.