2010 Cilt 29 Sayı 2

Permanent URI for this collectionhttps://hdl.handle.net/11452/13939

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    Replacement of fetal calf serum with two different synthetic sera in in vitro maturation medium: effects on maturation, fertilization and subsequent development of cattle oocytes in vitro
    (Uludağ Üniversitesi, 2010-12-29) Sağırkaya, Hakan; Üstüner, Burcu
    The aim of this study was to investigate the effect of using synthetic sera (synthetic serum substitute, SSS and serum replacement 1, SR1) instead of fetal calf serum (FCS) in in vitro maturation medium on in vitro maturation (IVM), fertilization (IVF) and subsequent development of bovine oocytes. Selected oocytes collected from ovaries obtained from a local slaughterhouse were matured in tissue culture medium 199 (TCM-199) supplemented with 2 mM glutamine, 0.25 mM Na-pyruvate, 0.5 g/ml FSH, 5 g/ml LH, 100 U/ml penicillin, 100 µg/ml streptomycin and 10% FCS, SSS or SR1 (FCS, SSS and SR1 groups, respectively) for 22 hours. Matured oocytes were in vitro fertilized using frozen bull sperm. Fertilization day was considered as day 0 in the present study. Eighteen hours after IVF, fertilized oocytes were transferred into synthetic oviduct fluid (SOF) culture medium for in vitro culture. Two cell, 8-cell, morulae and blastocyst numbers were recorded. A total of 238, 243 and 200 oocytes were used for FCS, SSS and SR1 groups, respectively. In FCS, SSS and SR1 groups, 179 (75.21%), 177 (72.84%) and 80 (40.00%); 121 (50.84%), 114 (46.91%) and 38 (19.00%); 97 (40.76%), 98 (40.03%) and 21 (10.50%); and 71 (29.83%), 69 (28.40%) and 10 (5.00%) oocytes developed to 2-cell, 8-cell, morulae and blastocyst stage embryos, respectively. The development rates of SR1 group at all recorded stages were significantly lower than those of FCS and SSS groups. There was no significant difference between FCS and SSS groups in terms of the developmental rates at any stage recorded. As a result, SSS is a good alternative for FCS replacement in in vitro maturation of bovine oocytes, however, SR1 is not a good alternative for FCS replacement in in vitro maturation medium.