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Browsing by Author "0000-0001-5141-0008"

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    Effect of sperm pooling with seminal plasma collected in breeding or nonbreeding season on Saanen goat sperm cryosurvival
    (Wiley, 2018-05) Üstüner, Burcu; Gökçe, Elif; Toker, M. Berk; Önder, Nail Tekin; Saǧırkaya, Hakan; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0003-4033-9749; 0000-0002-1438-221X; 0000-0001-5141-0008; AAG-7238-2021; A-2794-2014; AAH-8821-2021; AAH-2635-2021; JAC-3152-2023; 18937724600; 56779799700; 56480349200; 55670728900; 6602400461; 6508060684
    The aim of this study was to determine the effects of both the removal of seminal plasma (SP) and the pre-freezing addition of seminal plasma collected during the breeding or nonbreeding season on goat sperm survival after thawing. Semen samples were pooled. One aliquot of pooled semen was used as a control group. Four aliquots were then centrifuged, and the SP was removed in Group I, pipetted but not removed in Group II, removed and then pooled for animals collected in the breeding season in Group III and removed and pooled for animals collected in the nonbreeding season in Group IV. Group samples were frozen and then were assessed for rates of sperm motility, plasma membrane functional integrity hypo-osmotic swelling test (HOST), defective acrosomes (FITC-PSA), DNA fragmentation (TUNEL) and mitochondrial membrane damage (Rhodamine 123). The results showed that pre-freezing addition of SP collected in breeding season maintained post-thaw sperm characteristics at 0hr better than SP removal group, but removing seminal plasma showed positive effects on spermatozoa, as incubation time increased to 5hr. In conclusion, the pre-freezing addition of seminal plasma did not maintain post-thaw goat sperm characteristics as successfully as in the groups with seminal plasma removed after an incubation period.
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    Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa
    (Elsevier, 2016-03-21) Alçay, Selim; Gökçe, Elif; Toker, M. Berk; Önder, N. Tekin; Üstüner, Burcu; Uzabacı, Ender; Gül, Zülfiye; Çavuş, Seda; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; 0000-0001-5141-0008; 0000-0002-7678-3289; 0000-0002-9634-0055; 0000-0002-8872-0074; AAF-9939-2020; AAG-7238-2021; 56099810300; 56779799700; 56480349200; 55670728900; 18937724600; 55347697800; 56086542900; 57188685710
    The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.
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    Long term incubation resilience of post-thaw ram semen diluted with lecithin-based extender supplemented with bovine serum albumin
    (Kafkas Üniversitesi, 2019-05) Gökçe, Elif; Alçay, Selim; Toker, Mehmed Berk; Önder, N. Tekin; Üstüner, Burcu; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Dölerme ve Suni Tohumlama Anabilim Dalı; 0000-0001-5141-0008; 0000-0002-1438-221X; 0000-0003-4033-9749; JJQ-9411-2023; A-2794-2014; JAC-3152-2023; AAG-7238-2021; AH-2635-2021; 56099810300; 56480349200; 55670728900; 18937724600; 6508060684
    The objective of the study was to determine the optimum concentration of BSA in lecithin-based extender for post-thawing quality and incubation resilience (0 h, 6 h and 10 h) of ram sperm. Ejaculates were collected from five rams via electro ejaculation. Ejaculates were mixed to obtain pooled semen.Then, pooled semen was diluted with soybean lecithin-based extender without BSA (control) or supplemented with different concentrations of BSA (2.5 mg/mL, 5 mg/mL, 7.5 mg/mL and 10 mg/mL), at a final concentration of 150x106 spermatozoon/mL. Sperm motility, plasma membrane functional integrity (HOST), mitochondrial activity (rhodamine123), capacitation status (CTC), and DNA integrity (TUNEL) were evaluated. At the 10 h incubation, motility, plasma membrane functional integrity and mitochondria! function were better preserved in the BSA5 group compared to the control group. It was determined that high doses of BSA (5 mg/mL, 7.5 mg/mL and 10 mg/mL) affected acrosome reaction.The highest acrosome reaction rates were obtained in BSA10 groups in 6 h and 10 h incubation (P<0.05). TUNEL assay demonstrated that there were no differences among groups for DNA fragmentation at post-thaw and during incubation periods. The study shows that BSA supplemented extenders may have beneficial effect on ram semen parameters at 0 h, 6 h and 10 h of incubation. The results of the present study demonstrated a remarkable advantage of using 5 mg/mL of BSA in 1% lecithin-based extender.
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    Royal jelly supplemented soybean lecithin-based extenders improve post-thaw quality and incubation resilience of goat spermatozoa
    (Elsevier, 2016-11-25) Alçay, Selim; Toker, M. Berk; Önder, N. Tekin; Gökçe, Elif; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-7678-3289; 0000-0001-5141-0008; 0000-0003-4033-9749; ABA-6294-2020; 56099810300; 56480349200; 56779799700; 55670728900
    The aim of the present study was to evaluate different concentrations of royal jelly (RJ) supplemented extenders for post-thaw quality and incubation resilience of goat spermatozoa. Semen samples were collected from five goats. Pooled semen were diluted with soybean lecithin-based extender without RJ (control) or supplemented with different concentrations (0.25, 0.5 and 0.75%) of RJ (RJ0.25, RJ0.5, RJ0.75 respectively), at a final concentration of 150 x 106 spermatozoon/mL. Semen samples were assessed for sperm motility, plasma membrane integrity using hypoosmotic swelling test (HOST) damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The addition of RJ (0.5%, 0.75%) led to higher percentages of subjective motilities (55.33 +/- 2.29%, 57.67 +/- 2.58%) compared to control and RJ0.25 groups (49.00 +/- 2,80%, 51.67 +/- 3.09%) (P < 0.05) following the freezethawing process. RJ0.5 and RJ0.75 groups had higher plasma membrane functional integrities (66.40 +/- 1.34%, 68.20 +/- 2.05%) and lower defected acrosome rates (24.60 +/- 3.36%, 23.80 +/- 2.27%) compared to the other groups (P < 0.05). DNA damaged spermatozoa in all groups were not significant (P > 0.05). In the end of incubation, motility and HOST rates of RJ0.5 (14.00 +/- 3.87%, 31.20 +/- 3.70%) and RJ0.75 (15.00 +/- 3.27%, 29.20 +/- 2.59%) groups were higher than control (8.00 +/- 2.54%, 18.20 +/- 3.11%) and RJ0.25 (9.00 +/- 2.07%, 20.60 +/- 2.88%) groups (P < 0.05). Also defected acrosome and DNA fragmation rates of RJ0.5 (32.20 +/- 1.30%, 5.4 +/- 0.55%) and RJ0.75 (29.20 +/- 1.30%, 5.80 +/- 0.45%) groups were significantly lower than control (38.80 +/- 0.84%, 7.40 +/- 1.34%) and RJ0.25 (39.80 +/- 2.05%, 7.00 +/- 1.58) groups. This study shows that RJ supplemented extenders have beneficial effect on goat sperm parameters at 0 h and 6 h of incubation.