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Permanent URI for this collectionhttps://hdl.handle.net/11452/21452

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Effect of pre/postconditioning at temporary clipping
(Türk Nöroşirürji Derneği, 2013-10-26) Taşkapılıoğlu, Mevlüt Özgür; Morali, Tuğba Güler; Ocakoğlu, Gökhan; Korfalı, Ender; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0001-5472-9065; ABB-8161-2020; HPH-3049-2023; AAW-5254-2020; HLG-6346-2023; AAH-5180-2021; 25936798300; 56729201700; 15832295800; 7004641343
Intracranial aneurysms and their treatment is one of the leading problems of neurosurgery that create high mortality and morbidity. The technique of safe clipping is as generally used depends on the temporary occlusion of the cerebral vasculature during surgery. However, there is no exact data about temporary clipping or timing of this procedure. Preconditioning by exposure to sublethal hypoxic stress, hours or days before severe hypoxia, decreases cell death, and this resistance of the brain to injury is known as ischemic tolerance. Brief alternating periods of reperfusion-reocclusion at the beginning of reperfusion is defined as postconditioning. Cerebral ischemic pre/postconditioning protects against stroke, but is clinically feasible only when the occurrence of stroke is predictable. Brief, repetitive occlusion and release of the main trunk of a vessel during early aneurysm surgery or before long-lasting temporary artery occlusion may protect the brain against later possible vasospasm/ischemia.
Effect of reoperation on survival of patients with glioblastoma
(Ege Üniversitesi Tıp Fakültesi, 2012) Bekar, Ahmet; Taşkapılıoğlu, Mevlüt Özgür; Güler, Tuğba Morali; Aktaş, Ulaş; Tolunay, Şahsine; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0001-5472-9065; AAW-5254-2020; ABB-8161-2020; AAI-1612-2021; 6603677218; 55123171000; 55123170500; 55122917500; 6602604390
Objective: Surgical resection is the most important part of glioblastoma treatment. The objective of this study was to determine the effect of reoperation on survival of patients with glioblastoma. Methods: Records of the glioblastoma patients operated between 2001 and 2010 by the senior author were analyzed retrospectively. Comparisons were made between patients who received ( Reoperation group, n=50) and who did not receive (Monooperation group, n=111) reoperation with regard to age, gender, tumor localization, number of operations and length of survival. Results: No significant difference was found between two groups in terms of age, gender, and tumor localization. Mean follow-up duration was 12.7 months (range: 1-96 month). Mean lengths of survival after the first operation were 26.7 +/- 4.0 months and 12.2 +/- 1.6 months in the reoperation and monooperation groups, respectively (p<0.001). Regression analysis revealed that reoperation was the only prognostic factor determining the survival in recurrent malignant glial tumors. Moreover, surgical site was shown to affect survival; rate of mortality in patients operated on temporal side was statistically greater than that in patients operated on parietal side (p=0.01). Conclusion: Despite modern treatment strategies, reoperation is still the most important factor determining the length of survival in recurrent glioblastoma.
Histologic and electromyographic evaluation of neuroregenerative effect of stromal vascular fraction following neuroanastomosis
(Kafkas Üniversitesi, 2020-03-23) Acar, Hilal; Çerçi, Ece; Khezri, Marzieh Karimi; Çetin, Melike; Canatan, Uygur; Şen, Mehmet Metin; Aslan, Vildan; Sarıl, Canan Altıncı; Temiz, Elif Mekik; Taşkapılıoğlu, Mevlüt Özgür; Erdost, Hatice; Salcı, Hakan; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.; 0000-0001-5472-9065; 0000-0003-0154-9938; 0000-0001-9650-0891; 0000-0001-5323-6891; 000-0003-1547-7293; 0000-0001-6548-8754; AAH-9216-2021; AAI-6861-2021; AAW-5254-2020; ABB-8161-2020; 57209653860; 57212212053; 57216947212; 56393942900; 57189443508; 57216950078; 57200994296; 57216954758; 57216945905; 25936798300; 6505787570; 8680329000
In this study, histologic and electromyographic (EMG) evaluation of neuroregenerative effect of stromal vascular fraction (SVF) following periferal nerve anastomosis was aimed. Totally, 31 Wistar Albino, male rats (weighing about 300 g) were studied, and these rats were grouped according to surgical techniques performed on the right sciatic nerve: group I (GRI) micro suture (n=7), group II (GRII) micro suture + SVF (n=7), group III (GRIII) fibrin glue (n=7) and group IV (GRIV) fibrin glue + SVF (n=7). Fat tissue was taken from 3 rats to prepare SVF, and SVF was produced by non-enzymatic method. The left sciatic nerve of all rats was evaluated for EMG as control. Under general anesthesia, after transversal incision of the sciatic nerve, microsurgical epineural repair technique was performed with 10/0 nonabsorbable suture. EMG examinations were performed in terms of conduction rate, amplitude, distal latency and spontaneous muscle activity at 0th day and postoperative (PO) 1st and 8th weeks. EMG results between and within the groups were statistically evaluated by one-way analysis of variance. Rats in all groups were sacrificed by decapitation at PO 8th week and histological examinations of the sciatic nerves were performed following preparation of the neural tissues. EMG examination results showed the highest nerve conduction in GRI, the highest amplitude in GRIII, normal latency in GRII and longer distal latency in GRIV at PO 8th week. Amplitude and conduction velocity increased gradually in all groups. In needle EMG, the best muscle membrane stabilization was achieved in GRII and GRIV at PO 8th week. Statistically, the values of amplitude, distal latency, conduction velocity, and spontaneous muscle activity were found to be at normal levels at PO 8th week in all groups (P>0.05). In the histological results, although fibroconnective tissue reactions in the anastomosis area had similar scores in GRII and GRIV, maximum fibroconnective tissue reaction and the best axonal regeneration was seen in GRI and GRIII, respectively. In addition, GRI and GRII had the most inflammatory cells accumulation in the suture region, and less inflammatory cells were seen in the anastomosis area of GRIII and GRIV. As a conclusion, fibrin glue presents good electrophysiological and histological results; however, it is clear that local SVF usage on the nerve anastomosis area can be a good choice to decrease fibroconnective tissue reaction and inflammation.
Investigation of the dose-dependency of citicoline effects on nerve regeneration and functional recovery in a rat model of sciatic nerve injury
(Türk Nöroloji Derneği, 2014-01) Kaplan, Tolga; Kafa, İlker Mustafa; Cansev, Mehmet; Bekar, Ahmet; Karlı, Necdet; Taşkapılıoğlu, Mevlüt Özgür; Kanar, Fulya; Uludağ Üniversitesi/Tıp Fakültesi/Beyin ve Sinir Cerrahisi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Anatomi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Nöroloji Anabilim Dalı.; 0000-0001-5472-9065; AAW-5254-2020; AAG-7125-2021; M-9071-2019; 7006761497; 8450193200; 8872816100; 6603677218; 6506587942; 25936798300; 56037464100
AIM: The aim of this study was to investigate the dose dependence of citicoline's previously-reported effects on recovery of peripheral nerve injury. MATERIAL and METHODS: Right sciatic nerves of sixty adult female Wistar Albino rats were incised and primary anastomosis was performed. Rats were then divided into four groups: Control group received 2 ml of saline intraperitoneally, while rats in C-300, C-600 and C-900 groups received 300 mu mol/kg, 600 mu mol/kg and 900 mu mol/kg citicoline dissolved in 2 ml saline, respectively. Rats were tested for sciatic functional index (SFI) on the 4th, 8th and 12th weeks and electrophysiological recordings were obtained on the 12th week. Rats were then sacrificed to investigate nerve adhesions and perform histomorphological examinations. RESULTS:Our results showed that rats in C-600 and C-900 groups had significantly lesser neural adhesion and greater SFI and electrophysiological score than those in the Control and C-300 groups (p<0.05). Mean density and total number of functionally myelinated axons were significantly increased in C-900 group, while perineural scar tissue formation was reduced in all citicoline-treated groups. CONCLUSION: We conclude that citicoline exhibits dose-dependent effects on axonal regeneration and recovery without scar formation in a rat model of peripheral nerve incision and primary anastomosis.
Multiple extracranial metastases from secondary glioblastoma: A case report and review of the literature
(Türk Nöroloji Derneği, 2013) Taşkapılıoğlu, Mevlüt Özgür; Aktaş, Ulaş; Eser Ocak, Pınar; Tolunay, Şahsine; Bekâr, Ahmet; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0001-5472-9065; 0000-0003-0132-9927; AAI-2073-2021; AAW-5254-2020; ABB-8161-2020; AAI-1612-2021; 25936798300; 55122917500; 55211742300; 6602604390; 6603677218
Glioblastoma represents extreme anaplasia in astrocytic tumors. In spite of this aggressiveness, extracranial metastasis of glioblastoma is very rare and has been documented in only a few patients in the literature. In this article, a 30-year-old woman with secondary glioblastoma associated with extracranial distant metastasis was presented. In September 2008, an intracranial lesion in the left frontal region was diagnosed by magnetic resonance imaging (MRI) after admission to the hospital by headache and seizure and subsequently resected. The histology of the lesion revealed an anaplastic astrocytoma (grade III). Upon recurrence of the tumor 7 months later, the patient underwent a second craniotomy for recurrence tumor resection. The histological diagnosis was glioblastoma. After radiotherapy and chemotherapy, cranial computerized tomography (CT) and whole body scintigraphy revealed metastatic lesions in the right cervical lymph nodes and the left ischium. A neck dissection and parathyroidectomy on the right side was performed. The cytomorphological and histological features of the tumor supported the diagnosis of metastatic glioblastoma.
Prevention of epidural fibrosis in rats by local or systemic administration of citicoline
(Turkish Neurosurgical Society, 2012-09) Ulus, İsmail Hakkı; Savran, Mehmet; Bekar, Ahmet; Cansev, Mehmet; Tolunay, Şahsene; Taşkapılıoğlu, Mevlüt Özgür; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0001-5472-9065; 0000-0003-2918-5064; AAW-5254-2020; M-9071-2019; ABB-8161-2020; 6506735836; 6603677218; 8872816100; 6602604390; 25936798300
AIM: The objective of this study was to investigate the effect of citicoline administration on epidural fibrosis which is a frequent complication of lumbar disc surgery with no effective treatment or preventive surgical technique. MATERIAL and METHODS: Sixty Sprague-Dawley female rats undergoing L4-5 right hemilaminotomy and annular fenestration were arranged in three groups: rats in Group 1 (control group) and Group 2 (topical citicoline group) were applied 0,9% saline and 100 mu M citicoline on surgical area, respectively, while rats in Group 3 (systemic citicoline group) received 600 mu mol/kg citicoline intraperitoneally. Rats were sacrificed four weeks later and their vertebral colons were removed en bloc. Groups were evaluated according to histological criteria and results were compared using statistical tools. RESULTS: Compared with control group, significantly less epidural fibrosis, dural adhesion, fibroblast cell density, foreign body reaction, and medulla spinalis retraction were observed in groups treated with topical and systemic citicoline (groups 2 and 3) (p < 0,001). No significant difference was found with regard to measured parameters between two treatment groups (p > 0,05). CONCLUSION: Our study demonstrates for the first time in the literature that citicoline may be effective for preventing postoperative epidural fibrosis. However, its mechanism of action and clinical effectiveness must be further investigated.
Targeting apoptosis through FOXP1, and N-cadherin with glatiramer acetate in chick embryos during neural tube development
(Türk Nöroşirürji Derneği, 2015-06-18) Billur, Deniz; Kızıl, Şule; Aydın, Sevim; Ünlü, Ağahan; Taşkapılıoğlu, M. Ozgur; Taşkapılıoğlu, Özlem; Ocakoğlu, Gökhan; Bekar, Ahmet; Uludağ Üniversitesi/Tıp Fakültesi/Nöroşirürji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Nöroloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0001-5472-9065; HLG-6346-2023; AAK-6623-2020; ABB-8161-2020; AAH-5180-2021; AAW-5254-2020; 25936798300; 23037226400; 15832295800; 6603677218
AIM: To demonstrate the effect of glatiramer acetate (GA) in chick embryos on neural tube (NT) development, and to explore its effects of FOXP1, apoptosis, and N-cadherin. MATERIAL and METHODS: One hundred fertile, specific pathogen free eggs were divided into 5 groups for this study. The eggshell was windowed specifically at 24 hours of incubation. The embryos in Group 1 (n=20) were treated with 10 mu l physiological saline; in Group 2 the embryos (n=20) were given 10 mu l GA (equal to daily human therapeutic dose); 20 mu l GA (equal to twice daily human therapeutic dose) was injected to embryos in Group 3 (n=20); in Group 4 and 5, 30 mu l and 40 mu l GA were administered to the embryos (n=20) (equal to x3 and x4 daily human therapeutic dose, respectively). Each egg was re-incubated for 24 hours more. Then, histological and immunohistochemical evaluation of the subjects were done. RESULTS: The embryos with NT defect showed FOXP1 expression without N-cadherin or staining with N-cadherin in another location in our study. We interpreted this result as GA leading to an NT closure defect by increasing FOXP expression. Moreover, we also showed the reverse relation between FOXP1 and N-cadherin at the immunohistochemical level for the first time. CONCLUSION: GA affects the spinal cord development through FOXP in the chick embryo model at high doses.

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