Browsing by Author "Özkan, Ömer Faruk"

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Effect of different doses of 2aEuroaminoethoxydiphenyl borate on intestinal ischemia-reperfusion injury
(European Surgery - Acta Chirurgica Austriaca, 2016-10-24) Başbuğ, Murat; Yıldar, Murat; Yaman, İsmail; Çavdar, Faruk; Özkan, Ömer Faruk; Aksit, Hasan; Aslan, Figen; Derici, Hayrullah; Özyiǧit, Musa Özgür; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; AAH-2873-2021; AAR-6478-2021; 6507338060
Background Acute mesenteric ischemia is a life-threatening clinical entity. 2-Aminoethoxydiphenyl borate (2-APB) is a membrane-permeable modulator of intracellular inositol triphosphate-induced calcium release. We investigated the effects of different 2-APB doses on intestinal ischemia-reperfusion injury in an experimental rat model. Methods We divided 24 Wistar albino rats into four groups: sham, control, ischemia-reperfusion +2 mg/kg 2-APB, and ischemia-reperfusion +4 mg/kg 2-APB. The sham group only underwent laparotomy for 1 h 30 min. A 30-min period of mesenteric ischemia was induced in the control and two treatment groups, followed by 1 h of reperfusion. Before the laparotomy, 2 mg/kg and 4 mg/kg 2-APB was administered i.v. in the treatments groups, and blood samples were collected after reperfusion. Serum levels of malondialdehyde, superoxide dismutase, glutathione, total antioxidant capacity, tumor necrosis factor (TNF)-alpha, and interleukin-6 were analyzed. Intestinal tissues were taken for histopathological, DNA fragmentation, and terminal deoxynucleotidyl transferase dUTP nick end labeling analyses to determine the proportion of apoptotic cells. Results 2-APB reduced serum malondialdehyde, TNF-alpha, and interleukin-6 levels. However, superoxide dismutase and total antioxidant capacity levels increased significantly in the 4-mg/kg 2-APB group (p < 0.05). The intestinal histopathological injury scores were significantly higher in the control group; these injuries were prevented in the 4-mg/kg 2-APB dose group. DNA damage after ischemia-perfusion decreased significantly in the 4-mg/kg 2-APB group compared with the control group. Conclusion 2-APB decreases oxidative stress and cell injury. Administering 4 mg/kg 2-APB prevented ischemia-perfusion injury by diminishing histological damage.
Effects of boric acid in an experimental rat model of hepatic ischemia-reperfusion injury
(Carbone Editore, 2015) Başbuğ, Murat; Yıldar, Murat; Yaman, İsmail; Özkan, Ömer Faruk; Akşit, Hasan; Çavdar, Faruk; Sunay, Fatma Bahar; Derici, Hayrullah; Özyiğit, Musa Özgür; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 0000-0003-0682-8127; AAH-2873-2021; 6507338060
Introduction: Hepatic ischemia-reperfusion injury can cause serious damages and affect distant organs. Boric acid is a antioxidant agent in ischemia/reperfusion injury. The aim of this study was to investigate the effects of boric acid in a rat model of hepatic ischemia-reperfusion injury. Material and methods: 30 rats were divided into three groups: sham, ischemia reperfusion and ischemia-reperfusion+boric acid. The sham group underwent only the surgical stress procedure. In the ischemia-reperfusion group, liver ischemia was induced by clamping the hepatic pedicle for 45 minute, followed by reperfusion for 1 hour. In the ischemia-reperfusion +boric acid group, the therapeutic agent boric acid was administered intraperitoneally, 10 minute before clamping the hepatic pedicle. Serum levels of malondialdehyde, superoxide dismutase, glutathione, total antioxidant capacity, tumor necrosis factor-alpha, interleukin-6, aspartate aminotransferase, alanine aminotransferase, Gamma-glutamyl transferase were determined. Liver tissues were taken for histopathological examination, DNA fragmentation, and TUNEL staining to determine the apoptotic index. Results: Boric acid moderately reduced serum levels of malondialdehyde, tumor necrosis factor-alpha, interleukin-6. aspartate aminotransferase, gamma-glutamyl transferase in the ischemia-reperfusion injury group. Superoxide dismutase and alanine aminotransferase levels were decreased significantly in the boric acid-administered group (P < 0.05). The histopathological injury scores and the rate of apoptosis were significantly higher in the ischemia-reperfusion group; these injuries were reduced by boric acid administration. Conclusion: Our results demonstrate that boric acid decreases lipid peroxidation and enhances the antioxidant defense mechanism. This study showed that boric acid might protect against ischemia-reperfusion injury in this rat model.
Is donor age an important factor in cadaveric organ donation?
(Aves, 2018-09-01) Dündar, Halit Ziya; Oflaz, Rafet; Çınar, Yavuz Selim; Sarkut, Pınar; Özkan, Ömer Faruk; Kaya, Ekrem; DÜNDAR, HALİT ZİYA; Oflaz, Rafet; Çınar, Yavuz Selim; Sarkut, Pınar; KAYA, EKREM; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; AAG-7319-2021; JIK-5424-2023; DJU-5362-2022; JJX-5826-2023; HKB-5363-2023
Introduction: Our liver transplantion center has determined that the organ donation rate is low in young donors. The goal of study was to investigate whether this observation was valid and the causes for rejecting organ donation among family members of young donors.Methods: The brain death declaration and organ donation rates were analyzed during the study period. The relationship between the organ donation and donor's age was investigated, and factors related to refusing organ donation were also analyzed. A questionnaire including 10 questions was prepared for family members of brain-dead patients, who rejected organ donation. The study was conducted by phone, and conversations were recorded.Results: Including the years between 2011 and 2015, there was a total of 750 brain death declarations, and 508 of the patients did not approve organ donation. The donor rate under 30 years was lower as compared to the elderly patients (23.8% vs. 32.9%, p=0.026). The most common reasons for refusing organ donation were religious in nature and the anxiety about the corruption of bodily integrity. Sixty-two percent of family members who refused organ donation declared that they would have accepted organ donation if the donor declared accepting organ donation when he or she was alive.Conclusion: For family members of patients who deceased at young ages emotional attitudes are very important about the decision of organ donation. The testament of person about organ donation is the most important factor affecting the decision of family members.