Browsing by Author "İleri, İrfan Kamuran"
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Item Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen(Polish Society of Veterinary Sciences, 2005-09) Ak, Kemal; İleri, İrfan Kamuran; Nur, Zekeriya; Sağırkaya, Hakan; Doǧan, İbrahim; Soylu, Mustafa Kemal; Uludağ Üniversitesi/Veteriner Fakültesi/Dölleme ve Suni Tohumlama Bölümü.; 0000-0002-1438-221X; AAH-8821-2021; 6508060684; 6602400461; 36762299000; 7003293300The objective of this study was to investigate the effect of low temperature thawing and post-thaw cold shock application on sperm motility, as well as acrosomal and plasma membrane integrities of cryopreserved bull semen. Frozen semen was thawed in a water bath at 5-7 degrees C for 30, 60 or 90 s (low thawed groups), cold shocked (at 5-7 degrees C for 30, 60, 90 s) after thawing at 37 degrees C for 30 s (cold shocked groups), cold for 30 s (control group). The thawing procedure affected the percentages of motile spermatozoa (P < 0.001)and damaged acrosome (P < 0.001) while the bull factor affected the percentages of motile spermatozoa (P < 0.01) and damaged acrosome (P < 00.01). However, swollen tail spermatozoa rates were not affected by the the two factors. There was a significant interaction between thawing procedures and bulls in terms of the studies sperm parameters (P < 0.001). In conclusion, the percentages of motility, intact acrosome and swollen tail spermatozoa of the control group were higher than low-temperature thawed and post-thaw cold-shocked groups. These results indicate that frozen spermatozoa are significantly sensitive to lower temperature not only before but also after thawing.Item Effects of different temperature treatments applied to deep stored bull semen on post-thaw cold shocked spermatozoa(National Veterinary Research Institute, 2006) İleri, İrfan Kamuran; Ak, Kemal; Nur, Zekariya; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; AAH-2635-2021; 6508060684Various treatments were applied to frozen semen during storage in containers with different nitrogen level and the effects of temperature changes due to these treatments on the resistance of spermatozoa against post-thaw cold shock were studied. Straws with frozen bull semen were placed into two identical field containers. One of these containers was the low nitrogen level container with 3 cm nitrogen level that is 2 cm above the lower end of straws and the other one was the high nitrogen level container with 17 cm nitrogen level that is 2 cm above the upper end of straws. Canister No. 1 in both containers served as control without any manipulation. Canister No. 2 were raised up to the neck of the container and kept at this level for 10 s. Canister No. 3 were taken out to such a position that their base was at the entrance of the container and kept at this level for 20 s. These manipulations were repeated 100 times for canisters No. 2 and 3, in both containers. Motility, defected acrosome, and hypo-osmotic swelling test were carried out at post-thaw (37 degrees C for 30 s) and after cold shock in a cold water bath (5-6 degrees C for 30 s). Post-thaw cold-shock was observed to cause significant damage to motility, membrane integrity, and acrosome integrity of spermatozoa. Low nitrogen level was not capable of protecting viability and morphological structures of spermatozoa. In container with low nitrogen level, raising the canister from the container and keeping it at this level for 20 s made the spermatozoa more sensitive to cold shock. In conclusion, container nitrogen level and canister manipulations had negative effects on both shocked and unshocked spermatozoa. Also the results provide evidence that not only canisters misuse but also low nitrogen level made spermatozoa more sensitive to post-thaw cold shock.