Browsing by Author "İnci, Duygu"
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Item Affinity of a new copper(II) complex to DNA/BSA and antioxidant/radical scavenging activities: Crystal structure of [Cu(4,7-diphenyl-1,10-phenanthroline)(leucine)(NO3)(H2O)](Taylor & Francis, 2016) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; G-2201-2019; AAH-8936-2021; 55082306300; 56261495600A new copper(II) complex, [Cu(Bphen)(Leu)(NO3)(H2O)] (Bphen=4,7-diphenyl-1,10-phenanthroline, leu=L-leucine), has been synthesized and characterized by IR spectroscopy, CHN analysis, and single-crystal X-ray diffraction techniques. The CT-DNA binding properties of the complex have been investigated by both absorption and emission spectroscopy. The binding parameters for the fluorescence Scatchard plot were also determined. Further, the interaction of the complex with bovine serum albumin (BSA) has been investigated using absorption and emission spectroscopy. The thermodynamic parameters, free energy change (G), enthalpy change (H), and entropy change (S), were calculated by the van't Hoff equation and discussed. The distance between BSA and the complex has been obtained according to fluorescence resonance energy transfer. Conformational changes of BSA have been observed from synchronous fluorescence. Antioxidant and radical scavenging activities of the complex were determined by various in vitro assays such as 1,1-diphenyl-2-picryl-hydrazyl free radicals (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS(+)), and reducing ability determination by H2O2 scavenging methods.Item Antiproliferative activity of copper(II) glutamine complexes with N,N-donor ligands: Synthesis, characterization, potentiometric studies and DNA/BSA interactions(Elsevier, 2019-05-21) Zorlu, Yunus; Kiraz, Sirem; İnci, Duygu; Aydin, Rahmiye; Vatan, Özgür; Cavaş, Tolga; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0003-1620-1918; 0000-0002-7687-3284; 0000-0002-0483-9642; 0000-0003-4944-0181; AAH-8936-2021; AAH-3508-2021; O-7508-2015; G-2201-2019New water soluble copper(II) complexes, - [Cu(phen)(gln)(H2O)]NO3 center dot H2O (1) and [Cu(dmphen)(gln)(H2O)]ClO4 (2) - (phen: 1,10-phenanthroline, dmphen: 4,7-dimethyl-1,10-phenanthroline, gln: L-glutamine), have been synthesized and characterized by CHN analysis, ATR-FT-MIR, ESI-MS and single-crystal X-ray diffraction techniques. Binary and ternary complexes of copper(II) with the selected ligands have been investigated using potentiometric methods in 0.1 M KCl aqueous ionic media at 298.2 K. The protonation constants of the selected ligands and the stability constants of the complexes 1 and 2 have been calculated from the potentiometric data using the "BEST" software package and the potentiometric results have been analyzed using the "SPE" software package. The binding interaction of the complexes with calf thymus DNA (CT-DNA) was investigated by electronic absorption and emission spectroscopic methods revealed that the complexes could interact with CT-DNA via a moderate intercalation mode. The fluorescence quenching mechanism of bovine serum albumin (BSA) by the complexes was analyzed and the binding constant has been calculated. In vitro antiproliferative effect of the complexes was examined on human tumor cell lines (Caco-2, A549 and MCF-7) and healthy cells (BEAS-2B). The complex 2 showed remarkable antiproliferative activity compared to the complex 1 and cisplatin.Item Bakır(II) iyonunun L-amino asitler ve aromatik diiminler ile oluşturduğu komplekslerin sentezi, yapılarının aydınlatılması, kararlılık sabitlerinin ve DNA etkileşimlerinin araştırılması(Uludağ Üniversitesi, 2013) İnci, Duygu; Aydın, Rahmiye; Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Kimya Anabilim Dalı.Bu çalışmada, biyolojik sistemlerde önemli oldukları için Cu(II) iyonu ve L-amino asitler (L-fenilalanin, L-tirozin, L-triptofan) ile düzlemsel yapılı diiminlerin(1,10-fenantrolin, 4,7-dimetil-1,10-fenantrolin, 5-nitro-1,10-fenantrolin) oluşturduğu kompleksler araştırıldı. İlk olarak, 25oC ve I=0,1 M KCl iyonik ortamında, sulu çözeltide oluşan ikili ve karışık ligant komplekslerinin kararlılık sabitleri, potansiyometrik yöntem ile belirlendi. Komplekslerin kararlılık sabitleri, potansiyometrik titrasyonlardan elde edilen verilerin BEST bilgisayar programına uygulanması ile hesaplandı. Karışık ligant sistemlerinde sadece metal ile ligandlar arasında etkileşim olduğu ve ligandların kendi aralarında etkileşimin olmadığı kabul edilerek logK değerleri bulundu. SPE bilgisayar programı ile çözelti ortamında bulunan türlerin dağılımı belirlendi.İkinci olarak, Cu(II) iyonunun 1,10-fenantrolin, 4,7-dimetil-1,10-fenantrolin, 5-nitro-1,10-fenantrolin ve L-tirozin ligandları ile oluşturduğu ikili ve karışık ligant kompleksleri sentezlendi. Elde edilen komplekslerin yapıları, elementel analiz, IR spektroskopisi ve tek kristal X-ışını kırınımı yöntemi ile aydınlatıldı ve komplekslerin termik davranışları araştırıldı. Üçüncü olarak, sentezlenen komplekslerin DNA ile etkileşimlerini belirlemek için, fizyolojik şartlarda, elektronik absorbsiyon spektroskopisi yöntemi kullanıldı ve komplekslerin DNA'ya bağlanma sabitleri(Kb) hesaplandı. Ayrıca, elde edilen komplekslerin akciğer kanser hücre örneklerinde sitotoksisite ölçümleri yapıldı.Item Bakır(II) ve palladyum(II) iyonlarının fenantrolin türevleri ve amino asitler ile oluşturduğu komplekslerin sentezi, karakterizasyonu, DNA/protein etkileşimlerinin ve biyolojik özelliklerinin araştırılması(Uludağ Üniversitesi, 2018-05-04) İnci, Duygu; Aydın, Rahmiye; Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Kimya Anabilim Dalı.Bu çalışmada, metal iyonu olarak bakır(II) ve palladyum(II) iyonları, fenantrolin türevi olarak 1,10-fenantrolin (phen), 4-metil-1,10-fenantrolin (4-mphen), 5-metil-1,10-fenantrolin (5-mphen), 4,7-dimetil-1,10-fenantrolin (dmphen), 3,4,7,8-tetrametil-1,10-fenantrolin (tmphen) ve pyrazino[2,3-f][1,10]fenantrolin (py-phen), amino asit olarak ise glisin (gly) ve L-tirozin (L-tyr) seçildi. Sulu çözelti çalışmalarında, bakır(II) ve palladyum(II) iyonlarının seçilen ligantlar ile oluşturduğu komplekslere ait denge tepkimeleri 25°C, 0,1 M KCl ve 0,1 M KNO3 iyonik ortamında potansiyometrik yöntem ile araştırıldı. Ligantların protonlanma sabitleri ile bakır(II) ve palladyum(II) komplekslerinin kararlılık (oluşum) sabitleri BEST bilgisayar programı kullanılarak hesaplandı. Çözeltide oluşan türlerin dağılımı ise SPE bilgisayar programı kullanılarak belirlendi. Sentez çalışmalarında, seçilen ligantların bakır(II) ve palladyum(II) iyonları ile oluşturduğu ikili ve karışık ligant kompleksleri sentezlendi. Sentezlenen komplekslerin yapıları elementel analiz, elektronik absorbsiyon spektroskopisi, IR, ESI-MS ve X-ışını tek kristal kırınım yöntemleri, Pd(II) komplekslerinin yapıları ise ilave olarak 1H NMR ve 13C NMR teknikleri ile aydınlatıldı. Biyolojik aktivite çalışmalarında, komplekslerin buzağı timüsü DNA'sı (CT-DNA) ile etkileşimleri elektronik absorpsiyon ve floresans spektroskopisi, termal denatürasyon ve agaroz jel elektroforez teknikleri kullanılarak incelendi. Komplekslerin sığır serum albümini (BSA) ile etkileşimleri elektronik absorpsiyon ve floresans (senkronize floresans, floresans rezonans enerji transferi (FRET), iki boyutlu (2D) ve üç boyutlu (3-D) floresans) spektroskopisi teknikleri kullanılarak araştırıldı ve BSA sönümleme mekanizması bulundu. Sentezlenen komplekslerin DPPH radikal süpürme ve H2O2 radikali yakalama aktiviteleri standart olarak kullanılan askorbik asit, trolox ve BHT ile karşılaştırmalı olarak araştırıldı. Komplekslerin antikanser aktiviteleri ise meme, akciğer ve kolon kanseri hücre hatları ile sağlıklı insan bronşiyal epitel hücre hattında cis-Platin ile karşılaştırmalı olarak belirlendi.Item Binary and ternary new water soluble copper(II) complexes of l-tyrosine and substituted 1,10-phenanthrolines: Effect of substitution on DNA interactions and cytotoxicities(Pergamon-Elsevier, 2015-06-15) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Vatan, Özgür; Yılmaz, Dilek; Gençkal, Hasene Mutlu; Cavaş, Tolga; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; 0000-0002-0026-7755; 0000-0003-1620-1918; 0000-0002-7687-3284; 0000-0002-0483-9642; AAH-8936-2021; AAH-2888-2021; AAH-3508-2021; O-7508-2015; G-2201-2019; 55082306300; 56261495600; 16235098100; 6701369462; 57212275330; 6602989548Binary and ternary water soluble copper(II) complexes - [Cu(nphen)(2)(H2O)](NO3)(2)center dot H2O (1), [Cu(phen)(2)(H2O)](NO3)(2) (2), [Cu(nphen)(L-tyr)(H2O)]NO3 center dot 2H(2)O (3), [Cu(Phen)(tyr)(H2O)] NO3 center dot 2H(2)O (4) - and diquarternary salts of nphen and phen (nphen = 5-nitro-1,10-phenanthroline, phen = 1,10-phenanthroline and tyr = L-tyrosine) have been synthesized and characterized by CHN analysis, H-1 NMR, C-13 NMR and IR spectroscopy, thermal analysis and single crystal X-ray diffraction techniques. The CT-DNA binding properties of these compounds have been investigated by thermal denaturation measurements, absorption and emission spectroscopy. The supercoiled pUC19 plasmid DNA cleavage activity of these compounds has been explored by agarose gel electrophoresis. The cytotoxicity of these compounds against MCF-7, Caco-2, A549 cancer cells and BEAS-2B healthy cells was also studied by using XTT method. The complexes 1-4 exhibit significant high cytotoxicity with low IC50 values in compared with cisplatin. The effect of the substituents of phen and coordinated amino acid in the above complexes are presented and discussed.Publication Biomacromolecular interactions and radical scavenging activities of one-dimensional (1D) copper(II) glycinate coordination polymer(Springer, 2021-05-18) İnci, Duygu; Aydın, Rahmiye; Zorlu, Yunus; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; 0000-0003-4944-0181; AAH-8936-2021One-dimensional (1D) copper(II) glycinate coordination polymer {[Cu(gly)(2)(H2O)](n); (gly: glycine)} has been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with biomacromolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes indicate that the complex binds to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between the complex and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (Delta G, Delta H, Delta S) of BSA + the complex systems were determined at different temperatures. The binding distance between the complex and BSA was calculated according to Forster non-radiation energy transfer theory (FRET). The effect of the complex on the conformation of BSA was also examined using synchronous, two-dimensional (2D) and three-dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complex was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interactions and radical activity studies, the complex was found to give good results.Publication Cu(ii) complex with auxin (3-indoleacetic acid) and an aromatic planar ligand: Synthesis, crystal structure, biomolecular interactions and radical scavenging activity(Springer, 2021-04-30) İnci, Duygu; Aydın, Rahmiye; Zorlu, Yunus; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; AAH-8936-2021A novel water soluble ternary copper(ii) complex,-[Cu-2(phen)(2)(3-IAA)(2)(H2O)](ClO4)(2)center dot H2O-(phen: 1,10-phenanthroline, 3-IAA: 3-indoleacetic acid), has been synthesized and characterized by elemental CHN analysis, ESI-TOF, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with calf thymus DNA (CT-DNA) has been investigated by absorption spectral titration, ethidium bromide (EB) and Hoechst 33258 displacement assay. The interactions between the complex and bovine serum albumin (BSA) were investigated by electronic absorption and fluorescence spectroscopy methods. The experimental results indicate that the fluorescence quenching mechanism between the complex and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (Delta G, Delta H, Delta S) of BSA + complex systems were determined at different temperatures. The binding distance between the complex and BSA was calculated according to Forster non-radiation energy transfer theory (FRET). The effect of the complex on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Furthermore, the oxygen radical scavenging activity of the complex was determined in terms of IC50, using the DPPH and H2O2 method, to show that it particularly enables electron loss from radical species. This study highlights the importance of indole and moieties in the development of antioxidant agents.Publication Equilibria studies on nickel(II) complexes with 1,10-phenanthroline derivatives and some amino acids in aqueous solution(Springer, 2021-01-02) İnci, Duygu; Aydın, Rahmiye; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; 0000-0002-0483-9642; AAH-8936-2021In recent years, an increase in the biological applications of metal complexes of biologically active ligands has been observed due to their key role in clinical treatments. A potentiometric titration technique has been used to determine the protonation constants of biologically active ligands {glycine (gly), leucine (leu), methionine (met), phenylalanine (phe), tryptophan (trp), asparagine (asn), glutamine (gln), 1,10-phenanthroline (phen), 4-methyl-phenanthroline (4-mphen), 5-methyl-phenanthroline (5-mphen), 3,4,7,8-tetramethyl-1,10-phenanthroline (3,4,7,8-tmphen), 5-nitro-phenanthroline (5-nphen)} and the stability constants of their (1:1) and (1:1:1) complexes with Ni(II) ion (I = 0.100 mol center dot L-1 KCl and T = 298.15 K). The protonation constants of the biologically active ligands and the stability constants of the (1:1) and (1:1:1) complexes of Ni(II) with the biologically active ligands have been computed by using the BEST program. Moreover, the SPE program has been used to determine the distribution of the species that occurred in the aqueous solution medium. The relative stabilities of the (1:1:1) Ni(II) complexes are compared with those of the corresponding (1:1) Ni(II) complexes in terms of Delta log(10)K values. The effect of phenanthroline substituents (4-methyl, 5-methyl, 3,4,7,8-tetramethyl, 5-nitro) on the nature of the Ni(II) complexes is evaluated.Item Interaction of a new copper(II) complex by bovine serum albumin and dipeptidyl peptidase-IV(Elsevier, 2018-09-28) Koseler, Aylin; Zeytunluoğlu, Ali; Zorlu, Yunus; Aydın, Rahmiye; İnci, Duygu; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; AAH-8936-2021; G-2201-2019; 55082306300; 56261495600Dipeptidyl peptidase-IV (DPP-IV) is one of the mammalian serine proteases participated in the pathogenesis of diseases and DPP-IV inhibitors are now widely used as antidiabetic drugs. A new water soluble ternary copper (II) complex,-[Cu(PY-Phen) (phe) (H2O)]NO3 center dot H2O-(py-phen:pyrazino[2,3f][1,10]phenanthroline, phe:phenylalanine), has been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Fluorescence spectroscopy was researched to study the interaction between the complex and bovine serum albumin (BSA) and dipeptidyl peptidase-IV (DPP-IV). Chromophore of BSA and DPP-IV enzyme is changed upon addition of the complex. Additionally, the complex was shown to have promising inhibitory activities against DPP-IV with lower IC50 value. This study may provide new insights into the development of effective agents against diabetes.Item Methyl substituent effect on one-dimensional copper(II) coordination polymers containing biologically active ligands: Synthesis, characterization, DNA interactions and cytotoxicities(Wiley, 2019-07-24) Zorlu, Yunus; Şenel, Pelin; İnci, Duygu; Aydın, Rahmiye; Huriyet, Huzeyfe; Çinkılıç, Nilüfer; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0003-4944-0181; 0000-0002-0483-9642; 0000-0002-3595-6286; 0000-0003-4495-8723; AAH-8936-2021; AAP-5959-2020; AAH-5296-2021; G-2201-2019; 55346251000; 55082306300; 57201093675; 26533892300; 56261495600Three novel water-soluble copper(II) complexes - {[Cu(phen)(trp)]ClO4 center dot 3H(2)O}(n) (1), {[Cu(4-mphen)(trp)]ClO4 center dot 3H(2)O}(n) (2) and [[Cu(dmphen)(trp)(MeOH)][Cu(dmphen)(trp)(NO3)]]NO3 (3) (phen: 1,10-phenanthroline; 4-mphen: 4-methyl-1,10-phenanthroline; dmphen: 4,7-dimethyl-1,10-phenanthroline; trp: l-tryptophan) - have been synthesized and characterized using various techniques. Complexes 1 and 2 are isostructural, and exist as one-dimensional coordination polymers. Complex 3 consists of two discrete copper(II) complexes containing [Cu(trp)(dmphen)(MeOH)](+), [Cu(trp)(dmphen)(NO3)] and one nitrate anion. The binding interaction of the complexes with calf thymus DNA (CT-DNA) was investigated using thermal denaturation, electronic absorption and emission spectroscopic methods, revealing that the complexes could interact with CT-DNA via a moderate intercalation mode. The binding activity of the complexes to CT-DNA follows the order: 3 > 2 > 1. The pUC19 DNA cleavage activity of the complexes was investigated in the absence and presence of external agents using the agarose gel electrophoresis method. Especially, in the presence of H2O2 as an activator, the pUC19 DNA cleavage abilities of the complexes are clearly enhanced at low concentration. Addition of hydroxyl radical scavenger dimethylsulfoxide shows a marked inhibition of the pUC19 DNA cleavage activity of the complexes. In vitro cytotoxic effect of the complexes was examined on human tumor cell lines (Caco-2, A549 and MCF-7) and healthy cells (BEAS-2B). The potent cytotoxic effect of complex 3, with IC50 values of 1.04, 1.16 and 1.72 mu M, respectively, is greater relative to clinically used cisplatin (IC50 = 22.70, 31.1 and 22.2 mu M) against the Caco-2, A549 and MCF-7 cell lines.Item New binary copper(II) complexes containing intercalating ligands: DNA interactions, an unusual static quenching mechanism of BSA and cytotoxic activities(Taylor and Francis, 2017-11-18) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Vatan, Özgür; Çinkılıç, Nilüfer; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0483-9642; 0000-0003-4944-0181; 0000-0002-7687-3284; G-2201-2019; AAH-8936-2021; O-7508-2015; 55082306300; 56261495600; 16235098100; 26533892300New binary copper(II) complexes - [Cu(4-mphen)(2)(NO3)]NO3 center dot H2O (1), [Cu(5-mphen)(2) (NO3)]NO3 center dot H2O (2), the known complex [Cu(dmphen)(2)(NO3)]NO3 (3) and [Cu(tmphen)(2) (NO3)]NO3 center dot H2O (4) - (4-mphen: 4-methyl-1,10-phenanthroline, 5-mphen: 5-methyl-1,10-phenanthroline, dmphen: 4,7-dimethyl-1,10-phenanthroline, tmphen: 3,4,7,8-tetramethyl-1,10-phenanthroline), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of these complexes with calf thymus DNA (CT-DNA) has been investigated by absorption spectral titration, ethidium bromide (EB) and Hoechst 33,258 displacement assay and thermal denaturation measurement. These complexes cleaved pUC19 plasmid DNA in the absence and presence of an external agent. Notably, in the presence of H2O2 as an activator, the cleavage abilities of these complexes are obviously enhanced at low concentration. Addition of hydroxyl radical scavengers like DMSO shows significant inhibition of the DNA cleavage activity of these complexes. BSA quenching mechanism was investigated with regard to the type of quenching, binding constant, number of binding locations and the thermodynamic parameters. The experimental results suggested that the probable quenching mechanism was an unusual static process and hydrophobic forces play a dominant role. The CT-DNA and BSA binding efficiencies of these complexes follow the order: 4 > 3 > 1 > 2. Furthermore, in vitro cytotoxicities of these complexes on tumor cells lines (Caco-2, MCF-7 and A549) and healthy cell line (BEAS-2B) showed that these complexes exhibited anticancer activity with low IC50 values. The effect of hydrophobicity of the methyl-substituted phenanthrolines on DNA and protein binding activities of these complexes is discussed.Item New water-soluble copper (II) complexes including 4,7-dimethyl-1,10-phenanthroline and l-tyrosine: Synthesis, characterization, DNA interactions and cytotoxicities(Pergamon-Elsevier, 2015-02-05) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Yılmaz, Dilek; Gençkal, Hasene Mutlu; Vatan, Özgür; Çinkiliç, Nilüfer; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.; 0000-0002-0026-7755; 0000-0002-3595-6286; 0000-0002-7687-3284; 0000-0002-0483-9642; AAH-2888-2021; AAH-5296-2021; O-7508-2015; AAH-8936-2021; G-2201-2019; 55082306300; 56261495600; 6701369462; 57212275330; 16235098100; 26533892300Two new water-soluble copper(II) complexes, [Cu(dmphen)(2)(NO3)]NO3 (1), [Cu(dmphen)(tyr)(H2O)] NO3 center dot H2O (2) and the diquartemary salt of dmphen (dmphen = 4,7-dimethyl-1,10-phenanthroline and tyr = L-tyrosine), have been synthesized and characterized by elemental analysis, H-1 NMR, C-13 NMR and IR spectroscopy, thermal analysis and single crystal X-ray diffraction techniques. The CT-DNA binding properties of these compounds have been investigated by absorption, emission spectroscopy and thermal denaturation measurements. The supercoiled pBR322 plasmid DNA cleavage activity of these compounds has been explored by agarose gel electrophoresis. The cytotoxicity of these compounds against MCF-7, Caco-2, A549 cancer cells and BEAS-2B healthy cells was also studied by the XTT method. Complexes 1 and 2 exhibit significant cytotoxicity, with lower IC50 values than those of cisplatin.Item Newly synthesized Cu(II) pyrazino[2,3-f][1,10]phenanthroline complexes as potential anticancer candidates(John Wiley and Sons, 2018-04) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Huriyet, Huzeyfe; Çinkılıç, Nilüfer; Uludağ Üniversitesi/Fen - Edebiyat Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0483-9642; 0000-0003-4944-0181; 0000-0002-3595-6286; G-2201-2019; AAH-8936-2021; AAH-5296-2021; CVI-9578-2022; 55082306300; 56261495600; 57201093675; 26533892300New binary and ternary copper(II) complexes, [Cu(py-phen)(2)(NO3)]NO3 (1), [Cu-2(py-phen)(2)(gly)(2)(NO3)(2)(H2O)(2)]3H(2)O (2) and [Cu-2(py-phen)(2)(tyr)(2)(H2O)(2)](NO3)(2)3H(2)O (3) (py-phen: pyrazino[2,3-f][1,10]phenanthroline; gly: glycine; tyr: tyrosine), have been synthesized and characterized using CHN analysis, electrospray ionization mass spectrometry, Fourier transform infrared spectroscopy and single-crystal X-ray diffraction. Interaction of these complexes with calf thymus DNA has been investigated using absorption spectral titration, ethidium bromide and Hoechst 33258 displacement assay and thermal denaturation measurements. These complexes were found to be efficient cleaving agents and cleavage reactions were mediated by hydrolytic and oxidative pathways. The interaction between these complexes and bovine serum albumin (BSA) was investigated using electronic absorption and fluorescence spectroscopy. The experimental results show that the fluorescence quenching mechanism of these complexes and BSA is a static quenching process. Furthermore, in vitro cytotoxicities of these complexes against tumour cell lines (Caco-2, MCF-7 and A549) and healthy cell line (BEAS-2B) showed that they exhibited anticancer activity with low IC50 values. These complexes were markedly active against the cell lines and can be good drug candidates that are effective and safe for healthy tissue.Publication Noo-type tridentate schiff base ligand and its one-dimensional cu(ii) coordination polymer: Synthesis, crystal structure, biomacromolecular interactions and radical scavenging activities(Elsevier, 2021-01-01) İnci, Duygu; Aydın, Rahmiye; Zorlu, Yunus; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; AAH-8936-2021N-salicylidene-2-amino5-chlorobenzoic acid, (H2L), NOO-type tridentate Schiff base and its one-dimensional Cu (II) coordination polymer, [Cu-2(H2L)(2)(MeOH)(n), have been synthesized and characterized by CHN analysis, FTIR, (HNMR)-H-1, C-13 NMR, ESI-MS and XRD techniques. Interaction of the H2L Schiff base ligand and its the Cu(II) coordination polymer with biomacramolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy. The experimental outcomes indicate that the H2L Schiff base ligand and its the Cu(II) coordination polymer bind to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between the H2L Schiff base ligand and its the Cu(II) coordination polymer and BSA is a static quenching process. Radical scavenging activity of the H2L Schiff base ligand and its the Cu(II) coordination polymer was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interaction and radical activity studies, the Cu(II) coordination polymer was found to give better results than free the H2L Schiff base ligand.Item A potent drug candidature of Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes with bioactive ligands: synthesis, crystal structures, biomolecular interactions, radical scavenging and cytotoxicities(Taylor and Francis, 2020-08-01) İnci, Duygu; Zorlu, Yunus; Aydın, Rahmiye; Vatan, Özgür; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-7687-3284; 0000-0003-4944-0181; O-7508-2015; AAH-8936-2021; 56261495600; 16235098100A novel ternary copper(II) complexes, - [Cu(py-phen)(asn)(NO3)(H2O)] (1) and [Cu(py-phen)(trp)(H2O)]NO3(2)- (py-phen: pyrazino[2,3-f][1,10]phenanthroline, asn: asparagine, trp: tryptophan), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complexes1and2with CT-DNA has been investigated by absorption spectral titration, EB and Hoechst 33258 displacement assay. The interaction between the complexes1and2and BSA was investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes indicate that the fluorescence quenching mechanism between the complexes1and2and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (Delta G, Delta H, Delta S) of BSA + complex systems were determined at different temperatures. The binding distance between the complexes1and2and BSA was calculated according to FRET. The effect of the complexes1and2on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complex was determined in terms of EC50, using the DPPH and H(2)O(2)method. The anticancer activities of the complexes1and2were investigated using an XTT assay against three cancer cell lines (MCF-7, Caco-2 and A549) and non-tumor cell line (BEAS-2B). A potent drug candidature of two new copper(II) complexes, - [Cu(py-phen)(asn)(NO3)(H2O)] (1) and [Cu(py-phen)(trp)(H2O)]NO3(2)- (py-phen: pyrazino[2,3-f][1,10]phenanthroline, asn: asparagine, trp: tryptophan), have been synthesized and characterized by CHN analysis, FTIR, ESI-MS and single-crystal X-ray diffraction techniques. The complexes have been tested for theirin vitrobiomolecular interactions by the spectroscopic methods. Furthermore, radical scavenging and anticancer activities of the complexes was also investigated.Item Potentiometric and spectrophotometric studies of the complexation of lanthanum(III) with adrenaline, noradrenaline, and dopamine(American Chemical Society, 2012-03) Aydın, Rahmiye; İnci, Duygu; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; G-2201-2019; AAH-8936-2021The interaction of lanthanum(III) with the catecholamines adrenaline (AD), noradrenaline (NAD), and dopamine (DP) has been investigated by potentiometric and spectrophotometric methods at 298.15 K in an I = 0.2 M KCl ionic medium. The protonation constants for the ligands and the stability constants of the La(III) complexes are calculated from the potentiometric data using "BEST" software. The LaHL and La(HL)(2) complexes are reported in 1:1 and 1:2 molar ratios from the potentiometric and spectrophotometric data. The species distribution diagrams were also calculated. In all of the catecholamine systems, the coordination of the ligands to La(III) occurs via the phenolate sites (O; OH), but one of the phenolate protons does not dissociate. With respect to the ligands, the stability constants of the La(III) complexes decrease in the following order: dopamine > adrenaline > noradrenaline. The stability constants of these lanthanum(III) complexes are lower than the analogous Y(III) complexes due to the lower ionic potential of La(III).Item Potentiometric studies on complexation of cu(ii) ion with methyl/nitro-substituted 1,10-phenanthrolines and selected amino acids(Springer, 2016-10-13) İnci, Duygu; Aydın, Rahmiye; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; G-2201-2019; AAH-8936-2021; 55082306300; 56261495600Protonation constants of methyl/nitro substituted 1,10-phenanthrolines {(m/n-sphen): 4-methyl-phenanthroline (4-mphen), 5-methyl-1,10-phenanthroline (5-mphen), 4,7-dimethyl-1,10-phenanthroline (dmphen), 3,4,7,8-tetramethyl-1,10-phenanthroline (tmphen) and 5-nitro-1,10-phenanthroline (5-nphen)] and the amino acids (aa) l-tyrosine (tyr) and glycine (gly), and their corresponding binary and ternary stability constants with Cu(II), were determined in aqueous 0.1 mol center dot L-1 KCl ionic media at 298.15 K. The protonation constants of the ligands and the stability constants of the binary and ternary complexes of Cu(II) with the ligands were calculated from the potentiometric data using the "BEST" software package. The species distribution diagrams were obtained using the "SPE" software package under the experimental conditions described. The order of stability of the ternary complexes in terms of the primary ligands is [Cu(tmphen)(aa)](+) > [Cu(dmphen)(aa)](+) > [Cu(4-mphen)(aa)](+) > [Cu(5-mphen)(aa)](+) > [Cu(5-nphen)(aa)](+). The stability constants of the ternary complexes decrease in the following order: [Cu(m/n-sphen)(gly)](+) > [Cu(m/n-sphen)(tyr)](+), which is identical to the sequence found for the binary complexes of Cu(II) with gly and tyr.Item Stabilities of the ternary complexes of copper(II) with substituted 1,10-phenanthrolines and some amino acids in aqueous solution(Springer/Plenum Publishers, 2014-04) İnci, Duygu; Aydın, Rahmiye; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; G-2201-2019; AAH-8936-2021; 55082306300; 56261495600In this study the binary and ternary complexes of copper(II) with substituted 1,10-phenanthrolines [s-phen: 1,10-phenanthroline (phen), 4,7-dimethyl-1,10-phenanthroline (dmphen) and 5-nitro-1,10-phenanthroline (nphen)] and l-amino acids [aa: l-phenylalanine (phe), l-tyrosine (tyr) and l-tryptophan (trp)] have been investigated using potentiometric methods in 0.1 mol center dot L-1 KCl aqueous ionic media at 298.2 K. The protonation constants of the ligands and the stability constants of the binary and ternary complexes of Cu(II) with the ligands were calculated from the potentiometric data using the "BEST" software package. It was inferred that the aromatic 1,10-phenanthrolines act as a primary ligand in the ternary complexes, while the oxygen and nitrogen donor-containing amino acids are secondary ligands. The observed values of Delta log(10) K indicate that the ternary complexes are more stable than the binary ones, suggesting no interaction takes place between the ligands in the ternary complexes. The magnitudes of the measured stability constants of all of the ternary complexes are in the order [Cu(s-phen)(trp)](+) > [Cu(s-phen)(tyr)](+) > [Cu(s-phen)(phe)](+), which is identical to the sequence found for the binary complexes of Cu(II) with the amino acids. When the substituted 1,10-phenanthroline is changed, the stability constants of the ternary complexes decrease in the following order: [Cu(dmphen)(aa)](+) > [Cu(phen)(aa)](+) > [Cu(nphen)(aa)](+).Publication Structures, hydrolysis, stabilities of palladium(II) complexes containing biologically active ligands and species distribution in aqueous solution(Elsevier, 2019-04-13) İnci, Duygu; Aydın, Rahmiye; İNCİ ÖZBAĞCI, DUYGU; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; 0000-0003-4944-0181; G-2201-2019; AAH-8936-2021[PdACl(2)](n-1)H2O complexes {A: 1,10-phenanthroline (phen), 4-methyl-phenanthroline (4-mphen), 5-methyl-1,10-phenanthroline (5-mphen), 4,7-dimethyl-1,10-phenanthroline (dmphen), 3,4,7,8-tetramethyl-1,10-phenanthroline (tmphen)} were synthesized and characterized by CHN elemental analysis, ATR-FT-MIR and ATR-FT-FIR, H-1 NMR and C-13 NMR spectral measurements. n is the number of crystal water molecules in the complexes. The n value for the complexes [Pd(phen)Cl-2], [Pd(4-mphen)Cl-2] and [Pd(5-mphen)Cl-2] is 1; for the complex [Pd(tmphen)Cl-2]H2O is 2; for the complex [Pd(dmphen)Cl-2]2H(2)O is 3. [PdACl(2)](n-1)H2O complexes were converted into aqua complexes derived from 1,10-phenanthroline, [PdA(H2O)(2)](2+), and investigated [PdAB](+) mixed ligand complexes formed between [PdA(H2O)(2)](2+) complexes and amino acids {B: glycine (gly) and tyrosine (tyr)}. Protonation constants of the gly and tyr, the acid dissociation constants of the coordinated water molecules in [PdA(H2O)(2)](2+) complexes and the stepwise stability constant of the [PdAB](+) mixed ligand complexes were determined in aqueous 0.1 M KNO3 ionic media at 298.15 K by potentiometric methods. The protonation constants of ligands, the acid dissociation constants of aqua complexes and the stepwise stability constants of mixed ligand complexes were calculated from the potentiometric data using the "BEST" software package. The concentration distribution of the various species formed in aqueous solution was obtained using the "SPE" software package under the experimental conditions described. The order of stepwise stability of the mixed ligand complexes in terms of the gly and tyr was [Pd(tmphen)(gly/tyr)](+) > [Pd(dmphen)(gly/tyr)](+) > [Pd(4-mphen)(gly/tyr)](+) > [Pd(phen) (gly/tyr)](+) > [Pd(5-mphen)(gly/tyr)](+). This study could contribute to a better understanding of the behavior of the palladium(II) complexes in biological systems.Item Synthesis and crystal structures of novel copper(II) complexes with glycine and substituted phenanthrolines: reactivity towards DNA/BSA and in vitro cytotoxic and antimicrobial evaluation(Springer, 2017-01) Zorlu, Yunus; Yerli, Yusuf; Coşut, Bünyemin; İnci, Duygu; Aydın, Rahmiye; Vatan, Özgür; Sevgi, Tuba; Yılmaz, Dilek; Demirkan, Elif; Cinkılıç, Nilüfer; Uludağ Üniversitesi/Fen-Edebiyet Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0483-9642; 0000-0002-7687-3284; 0000-0002-7528-9529; 0000-0002-3595-6286; G-2201-2019; AAH-8936-2021; O-7508-2015; AAG-7112-2021; ABI-4472-2020; AAH-5296-2021; 55082306300; 56261495600; 16235098100; 57191880859; 6701369462; 23469245200; 26533892300New copper(II) complexes-dimeric-[Cu(nphen)(gly)(H2O)](+) (1) and [Cu(dmphen)(gly)(NO3)(H2O)] (2) (nphen = 5-nitro-1,10-phenanthroline, dmphen = 4,7-dimethyl-1,10-phenanthroline, and gly = glycine)-have been synthesized and characterized by CHN analysis, single-crystal X-ray diffraction techniques, FTIR, EPR spectroscopy, and cyclic voltammetry. The CT-DNA-binding properties of these complexes have been investigated by thermal denaturation measurements and both absorption and emission spectroscopy. The DNA cleavage activity of these complexes has been studied on supercoiled pUC19 plasmid DNA by gel electrophoresis experiments in the absence and presence of H2O2. Furthermore, the interaction of these complexes with bovine serum albumin (BSA) has been investigated using absorption and emission spectroscopy. The thermodynamic parameters, free-energy change (Delta G), enthalpy change (Delta H), and entropy change (Delta S) for BSA + complexes 1 and 2 systems have been calculated by the van't Hoff equation at three different temperatures (293.2, 303.2, and 310.2 K). The distance between the BSA and these complexes has been determined using fluorescence resonance energy transfer (FRET). Conformational changes of BSA have been observed using the synchronous fluorescence technique. In addition, in vitro cytotoxicities of these complexes on tumor cell lines (Caco-2, A549, and MCF-7) and healthy cells (BEAS-2B) have been examined. The antimicrobial activity of the complexes has also been tested on certain bacteria cells. The effect of mono and dimeric in the above complexes is presented and discussed. New copper(II) complexes-dimeric-[Cu(nphen)(gly)(H2O)](+) (1) and [Cu(dmphen)(gly) (NO3)(H2O)] (2) (nphen = 5-nitro-1,10-phenanthroline, dmphen = 4,7-dimethyl-1,10-phenanthroline and gly = glycine)-have been synthesized and characterized by CHN analysis, single-crystal X-ray diffraction techniques, FTIR and EPR spectroscopy. They have been tested for their in vitro DNA/BSA interactions by the spectroscopic methods. These complexes exhibited higher cytotoxic and antimicrobial activities. Complex 1 shows better DNA / BSA interactions in comparison to complex 2.