Browsing by Author "Abdou, Maoulida"
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Item Bacillus sp. suşlarından lipaz taranması, lipaz üretim koşullarının optimizasyonu, kısmi saflaştırılması, karakterizasyonu ve deterjan endüstrisindeki potansiyel uygulamaları(Bursa Uludağ Üniversitesi, 2019-07-12) Abdou, Maoulida; Demirkan, Elif; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Biyoloji Anabilim Dalı.Bu çalışmada, 341 Bacillus sp. suşundan 141'i hücre dışı lipaz üreticisi olarak bulunmuştur. En iyi lipaz üreten suş 16S rRNA ile tanımlandı ve Bacillus cereus ATA179 olarak adlandırıldı. Fiziksel parametreler arasında en iyi sonuçlar 45 ° C, pH 7.0, çalkalama hızı 50 rpm, aşılama miktarı %7 ve aşılama yaşı 24 saat idi. En iyi karbon kaynağı olarak sükroz, en iyi nitrojen kaynağı olarak (NH4) 2HP04, en iyi metal iyonu olarak CaCl2 elde edildi. Fiziksel parametreler arasında en iyi sonuçlar 45 ° C, pH 7.0, çalkalama hızı 50 rpm, aşılama miktarı %7 ve aşılama yaşı 24 saat olarak belirlenmiştir. ATA179 suşu, besinsel ve fiziksel koşulları optimize ederek yaratılan modifiye ortamda enzim üretiminde %51,1 artış göstermiştir. Kısmen saflaştırılmış enzimin optimum sıcaklığı ve pH değeri, sırasıyla 55 ° C ve 6.0 idi. Termostabilite ve pH stabilite çalışmaları, enzimin termostabil ve asidofilik olduğunu göstermiştir. CaCl2, Tween 20, Triton X-100, enzim aktivitesi üzerinde aktifleştirici bir etkiye sahipti. Vmax ve Km kinetik değerleri sırasıyla 18,28 U / ml ve 0,11 mM olarak bulunmuştur. Moleküler ağırlık yaklaşık 47 kDa olarak belirlenmiştir. Enzim aktivitesinin -20ºC'de 75 güne kadar stabil kaldığı bulunmuştur. Enzimin deterjan sanayiinde potansiyel gücü araştırılmış ve enzimin deterjan katkı maddelerinden etkilenmediği ayrıca yağlı maddelerle kirletilmiş kumaşlarda yağın çıkarılmasında etkili olduğu saptanmıştır. ATA179 bakterilerinin lipazı deterjan endüstrisinde kullanılma potansiyeline sahip olabilir.Publication Lipase from new isolate bacillus cereus ATA179: Optimization of production conditions, partial purification, characterization and its potential in the detergent industry(Tübitak Bilimsel ve Teknolojik Araştırma Kurumu, 2021-01-01) Demirkan, Elif; Aybey Çetinkaya, Aynur; Abdou, Maoulida; DEMİRKAN, ELİF; Aybey Çetinkaya, Aynur; Abdou, Maoulida; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü; 0000-0002-5292-9482; 0000-0003-2743-9745; ABI-4472-2020; CJN-1872-2022; CAU-1487-2022In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NHIHPO, as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45 degrees C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h. ATA179 strain showed a 51% increase in enzyme production in the modified medium created by optimizing nutritional and physical conditions. Optimum pH value and temperature were found as 6.0 and 55 degrees C, respectively. CaCl2, Tween 20, Triton X-100 had an activating effect on enzyme activity. V-max and K-m kinetic values were found as 18.28 U/mL and 0.11 mM, respectively. The molecular weight was determined as 47 kDa. Lipase was found to be stable up to 75 days at -20 degrees C. The potential of the enzyme in detergent industry was also investigated. It was not affected by detergent additives, but was found to be effective in removing oils from contaminated fabrics. This new lipase may have potential to be used in detergent industry.Item Strain improvement by UV mutagenesis for protease overproduction from bacillus subtilis E6-5 and nutritional optimization(Bursa Uludağ Üniversitesi, 2018-08-01) Demirkan, Elif; Sevgi, Tuba; Gokoz, Meltem; Guler, Baran Enes; Zeren, Behice; Ozalpar, Busra; Abdou, Maoulida; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.The purpose of the present study was to enhance the protease production of the parental type Bacillus subtilis E6-5 by UV irradiation. The parental type was subjected to UV irradiation at different distances (5-15 cm) for different time intervals ( 1-120 min). After each treatment, total 400 mutants were obtained. The mutants were screened on skim milk agar plates for the selection of best proteolytic mutant. Among mutants, the mutant MET39, which obtained at 15 cm distance and irradiation time 5 min of exposure, was selected as best mutant produced 1.5 fold more enzyme over the parent strain. The effects of nutritional factors (various carbon, nitrogen sources and metal ions) on the protease production from MET39 mutant strain were studied. The best carbon source was found as glycerol. Among the inorganic nitrogen sources, the highest enzyme production was obtained in the presence of tryptone. The metal ions did not indicate significant effect on enzyme production. In order to enhance the yield, new modified medium was obtained by combining the best carbon and nitrogen sources. In this medium, enzyme yield was enhanced 88% compared to basal medium. MET39 mutant strain might have a great potential for protease production at industrial scale.