Browsing by Author "Alsulimani, Ahmad"
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Publication Antineoplastic action of sulforaphane on hela cells by modulation of signaling pathways and epigenetic pathways(Edizioni Minerva Medica, 2021-12-01) Sundaram, Madhumitha Kedhari; Almutary, Abdulmajeed G.; Alsulimani, Ahmad; Ahmad, Syed Rehan; Somvanshi, Pallavi; Bhardwaj, Tulika; Pellicano, Rinaldo; Fagoonee, Sharmila; Hussain, Arif; Haque, Shafiul; Haque, Shafiul; Bursa Uludağ Üniversitesi/Tıp Fakültesi.; 0000-0002-2989-121X; AAN-2946-2020BACKGROUND: Epigenetic modifications alter signaling and molecular pathways; moreover, they are an important therapeutic target. This study examined the effect of sulforaphane on molecular targets in HeLa cells.METHODS: Quantitative PCR of various molecular targets was performed. Activity of epigenetic enzymes was measured by ELISA and molecular docking analysis was conducted. Promoter methylation of some tumor suppressor genes was quantified using PCR based methylation array. In-silico protein-protein interaction network analysis was performed to understand the effect of transcriptional changes.RESULTS: Quantitative PCR demonstrated the transcriptional modulation of genes involved in proliferation, metastasis, inflammation, signal transduction pathways and chromatin modifiers. Sulforaphane reduced the enzymatic activity of DNA methyl transferases, histone deacetylases and histone methyltransferases. Molecular docking results suggest that sulforaphane competitively inhibited several DNA methyl transferases and histone deacetylases. Promoter 5'CpG methylation levels of selected tumor suppressor genes was found to be reduced which correlated with their transcriptional increase as well modulation of epigenetic enzymes. Further, protein-protein interaction network analysis discerned the participation of genes towards cancer pathways. Functional enrichment and pathway-based analysis represented the modulation of epigenetic and signaling pathways on sulforaphane treatment.CONCLUSIONS: The modulation in transcriptional status of epigenetic regulators, genes involved in tumorigenesis resulting in tumor suppressor genes demethylation and re-expression underscores the mechanism behind the anticancer effect of sulforaphane on HeLa cells.Publication Phytochemicals from indian ethnomedicines: Promising prospects for the management of oxidative stress and cancer(MDPI, 2021-10-01) Fatima, Nishat; Baqri, Syed Shabihe Raza; Alsulimani, Ahmad; Fagoonee, Sharmila; Slama, Petr; Kesari, Kavindra Kumar; Roychoudhury, Shubhadeep; Haque, Shafiul; Haque, Shafiul; Bursa Uludağ Üniversitesi/Tıp Fakültesi; AAN-2946-2020Oxygen is indispensable for most organisms on the earth because of its role in respiration. However, it is also associated with several unwanted effects which may sometimes prove fatal in the long run. Such effects are more evident in cells exposed to strong oxidants containing reactive oxygen species (ROS). The adverse outcomes of oxidative metabolism are referred to as oxidative stress, which is a staple theme in contemporary medical research. Oxidative stress leads to plasma membrane disruption through lipid peroxidation and has several other deleterious effects. A large body of literature suggests the involvement of ROS in cancer, ageing, and several other health hazards of the modern world. Plant-based cures for these conditions are desperately sought after as supposedly safer alternatives to mainstream medicines. Phytochemicals, which constitute a diverse group of plant-based substances with varying roles in oxidative reactions of the body, are implicated in the treatment of cancer, aging, and all other ROS-induced anomalies. This review presents a summary of important phytochemicals extracted from medicinal plants which are a part of Indian ethnomedicine and Ayurveda and describes their possible therapeutic significance.