Browsing by Author "Ayaz, Fatma"
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Item Bioassay-guided isolation of cytotoxic compounds from Chrysophthalmum montanum (DC.) Boiss(Elsevier, 2018-12-21) Ayaz, Fatma; Küçükboyacı, Nurgün; Gören, Nezhun Ates; Çalış, İhsan; Ulukaya, Engin; Duman, Hayri̇ D.; Choudhary, Mohammed Iqbal; Aydınlık, Şeyma; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-5238-2432; ABI-2909-2020; 57190280044Bioassay-guided isolation of the 80% methanol extract of the aerial parts of Chrysophthalmum montanum (DC.) Boiss. (Asteraceae) led to the isolation of four known guaianolide-type sesquiterpene lactones, 6 alpha-acetoxy-4 alpha-hydroxy-113H-guaia-9.11(13)-dien-12.8a-olide (1), 6 alpha-acetoxy-4 alpha-hydroxy-9 beta.10 beta-epoxy-1 beta H-guaia-11(13)-en12.8 alpha-olide (2), 4 alpha,6 alpha-dihydroxy-1 beta H,5 alpha,7 alpha H-guaia-9(10),11(13)-dien-12,8 alpha-olide (3), and (4 alpha,5 alpha,8 beta,10 beta)-4,10-dihydroxy-1,11(13)-guaidien-12,8-olide (4), along a steroidal glycoside mixture (5a and 5b). The structures of the compounds were identified on the basis of spectroscopic data. Among them, 2, 4 and a steroidal glycoside mixture were obtained from C. montanum for the first time. All isolates were also first time assayed for in vitro cytotoxicities against four human cancer cell lines, i.e. breast (MCF-7, MDA-MB 231), colon (FIT-29), and lung (PC3). Among the isolates, 1-3 showed significant inhibitory effect on the proliferation of cancer cells with viability ranging from 6.86 to 26.51%, while steroidal glycoside mixture showed no cytotoxicity, except against HT-29 (viability 61.99%). Compound 4 exhibited strong and selective cell growth inhibition against HT-29 with viability 20.99% and was identified as a promising compound with high selectivity between cancer cells and normal human lung cells (SEAS-2B), especially against HT-29 (IC50 = 12.2 pg/mL) compared to that of cisplatin. These results suggested that 4 is worthy of further study to determine its cytotoxicity mechanisms.Publication Cytotoxic effect of conyza canadensis (l.) cronquist on human lung cancer cell lines(Galenos Publ House, 2016-12-01) Ayaz, Fatma; Küçükboyacı, Nurgün; Ulukaya, Engin; SARIMAHMUT, MEHMET; Sarımahmut, Mehmet; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0003-2647-5875; JCN-7113-2023Asteraceae family plants are receiving great attention because they have potential anticancer activity. Therefore, in this study, the cytotoxic effect of Conyza canadensis (L.) Cronquist (Asteraceae) were tested against human lung adenocarcinoma cell lines (A549 and H1299) for the first time. Cytotoxic effect of the n-hexane, chloroform, n-butanol and remaining water (R-H2O) extracts fractioned from the methanol extracts of the aerial parts and roots of C. canadensis was investigated using Sulforhodamine B (SRB) assay and percent (%) viability was measured. The results indicate that the extracts of C. canadensis have cytotoxic activities on these cells in a dose-dependent manner. The root extracts exhibited relatively higher cytotoxic effects than the aerial parts of the plant. The most active extract was found to be n-hexane extract of the roots with IC50 values 94.73 and 84.85 mu g/mL on A549 and H1299 cell lines, respectively. These results suggest that C. canadensis exhibits moderate cytotoxic effect in lung cancer cells. This might be taken into account in its use for therapeutic purposes.