Browsing by Author "BUDAK, FERAH"
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Publication Association of cytotoxic T lymphocyte subsets with disease severity in Covid-19(Wiley, 2021-08-01) Kızmaz, Muhammed Ali; Çağan, Eren; Şimşek, Abdurrahman; Dombaz, Fatma; Tezcan, Gülçin; Aşan, Ali; Demir, H. İbrahim; Bal, S. Haldun; Ermiş, Diğdem Yöyen; Coşkun, Necmiye Funda; Akalın, E. Halis; Oral, Haluk Barbaros; Budak, Ferah; Kızmaz, Muhammed Ali; ŞİMŞEK, ABDURRAHMAN; Dombaz, Fatma; TEZCAN, GÜLÇİN; Demir, H. İbrahim; BAL, SALİH HALDUN; YÖYEN ERMİŞ, DİĞDEM; COŞKUN, NECMİYE FUNDA; AKALIN, EMİN HALİS; ORAL, HALUK BARBAROS; BUDAK, FERAH; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Diş Hekimliği Fakültesi/Temel Bilimler Bölümü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Klinik Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; 0000-0001-5334-7911; 0000-0001-8850-0269; 0000-0001-7288-3250; 0000-0002-5956-8755; 0000-0002-8856-7356; 0000-0001-7585-7971; 0000-0003-3604-8826; 0000-0001-7530-1279; 0000-0003-0463-6818; 0000-0001-7625-9148; 0000-0003-3604-8826; HKN-2347-2023; IZP-9398-2023; AAH-3843-2020; F-4657-2014; AAG-7381-2021; K-7285-2012; AAU-8952-2020; DWR-5356-2022; GPN-1473-2022; KBR-5535-2024; GYL-2038-2022; AAD-1271-2019Publication Clinical, functional characteristics and exercise capacity of the frequent exacerbator copd phenotype(Amer Thoracic Soc, 2015-01-01) Uzaslan, Esra; Dilektaşlı, Aslı Görek; Çetinoğlu, Ezgi Demirdogen; Budak, Ferah; Coşkun, Funda; Ursavas, Ahmet; Ege, Ercüment; UZASLAN, AYŞE ESRA; GÖREK DİLEKTAŞLI, ASLI; Çetinoğlu, Ezgi Demirdogen; BUDAK, FERAH; COŞKUN, NECMİYE FUNDA; URSAVAŞ, AHMET; Ege, Ercüment; Uludağ Üniversitesi; 0000-0001-7099-9647; 0000-0002-7400-9089; 0000-0001-7625-9148; 0000-0003-3604-8826; JPK-7012-2023; F-4657-2014; AAD-1271-2019; AAI-3169-2021; IZP-9398-2023; CDI-1977-2022; DTT-7416-2022; AAH-9812-2021Publication Effect of storage period of erythrocyte suspensions on the CD4 & CD8 T cells(Wiley, 2021-08-01) Bal, Salih Haldun; Kumaş, Levent Tufan; Cevhertaş, Laçin; Yılmaz, İzel; Ellergezen, Pınar Hız; Budak, Ferah; Heper, Yasemin; Göral, Güher; Oral, Haluk Barbaros; BAL, SALİH HALDUN; Kumaş, Levent Tufan; Cevhertaş, Laçin; Yılmaz, İzel; ELLERGEZEN, PINAR; BUDAK, FERAH; HEPER, YASEMİN; Göral, Güher; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Dr. Rasit Durusoy Kan Bankası.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Mikrobiyoloji-İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Klinik Mikrobiyoloji Anabilim Dalı.; 0000-0003-2287-3569; 0000-0001-7625-9148; 0000-0003-0463-6818; F-4657-2014; IZP-9398-2023; K-7285-2012; JSK-9450-2023; KBR-5535-2024; FHB-1791-2022; FYD-1431-2022; CPH-1647-2022; CTY-9474-2022; JGX-8396-2023Publication Evaluation of indoleamine 2, 3 dioxygenase (IDO) gene polymorphisms in COVID-19(Wiley, 2021-08-01) Karaca, Mert; Arslan, Gözde; Ermiş, Diğdem Yöyen; Bal, Salih Haldun; Uzaslan, Esra Kunt; Özkalemkaş, Fahir; Macunluoğlu, Aslı Ceren; Budak, Ferah; Akalın, Halis; Oral, Haluk Barbaros; KARACA, MERT; Arslan, Gözde; YÖYEN ERMİŞ, DİĞDEM; BAL, SALİH HALDUN; Uzaslan, Esra Kunt; ÖZKALEMKAŞ, FAHİR; Macunluoğlu, Aslı Ceren; BUDAK, FERAH; AKALIN, EMİN HALİS; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biostatik Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı.; 0000-0002-6802-5998; 0000-0001-7625-9148; 0000-0001-7530-1279; 0000-0003-0463-6818; 0000-0002-6802-5998; 0000-0001-6711-676X; IZP-9398-2023; AAU-8952-2020; K-7285-2012; F-4657-2014; JFS-2013-2023; AAG-7406-2021; GYL-2038-2022; KBR-5535-2024; AAI-1004-2021; JLE-5241-2023; GBP-6589-2022Publication Evaluation of one year, five years and ten years life time of patients with kidney transplant: Single-center experience(Lippincott Williams & Wilkins, 2020-09-01) Elgin, Ersin; Aydın, Mehmet Fethullah; Ünsal, Oktay; Yıldız, Abdulmecid; Oruç, Ayşegül; Günseren, Kadir Ömur; Çiçek, Mehmet Çağatay; Budak, Ferah; Oral, H. Barbaros; Ersoy, Alparlan; Elgin, Ersin; Aydın, Mehmet Fethullah; Unsal, Oktay; Yıldız, Abdulmecid; ORUÇ, AYŞEGÜL; GÜNSEREN, KADİR ÖMÜR; ÇİÇEK, MEHMET ÇAĞATAY; BUDAK, FERAH; ORAL, HALUK BARBAROS; Ersoy, Alparlan; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Bursa Bölge Koordinasyon Merkezi.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Nefroloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Üroloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; 0000-0002-9509-5799; 0000-0002-5665-7402; 0000-0002-0342-9692; 0000-0001-7625-9148; 0000-0003-0463-6818; F-4657-2014; K-7285-2012; AAH-4002-2021; AAJ-8220-2020; IZP-9398-2023; DXA-2790-2022; JKA-8956-2023; JJY-8484-2023; EIF-8983-2022; JJO-6552-2023; CNT-9025-2022Publication Evaluation of the potential roles of activin-A, activin-B, and follistatin molecules exhibiting immunomodulatory properties in brucellosis(Wiley, 2021-08-01) Kızmaz, Muhammed Ali; Ellergezen, Pınar Hız; Demir, Nesrin; Çağan, Eren; Akalın, Emin Halis; Oral, Haluk Barbaros; Budak, Ferah; Kızmaz, Muhammed Ali; ELLERGEZEN, PINAR; AKALIN, EMİN HALİS; ORAL, HALUK BARBAROS; BUDAK, FERAH; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Klinik Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; 0000-0001-5334-7911; 0000-0001-7530-1279; 0000-0003-0463-6818; 0000-0001-7625-9148; AAU-8952-2020; F-4657-2014; K-7285-2012; HKN-2347-2023; IZP-9398-2023Publication Evaluation of the roles of regulatory B (Breg) cells and B cell exhaustion in COVID-19(Wiley, 2021-08-01) Budak, Ferah; Çağan, Eren; Kızmaz, Muhammed Ali; Şimşek, Abdurrahman; Dombaz, Fatma; Tezcan, Gülçin; Asan, Ali; Bal, S. Haldun; Ermiş, Diğdem Yöyen; Demir, H. İbrahim; Ediger, Dane; Yılmaz, Emel; Oral, Haluk Barbaros; Akalın, E. Halis; BUDAK, FERAH; Kızmaz, Muhammed Ali; ŞİMŞEK, ABDURRAHMAN; Dombaz, Fatma; TEZCAN, GÜLÇİN; BAL, SALİH HALDUN; YÖYEN ERMİŞ, DİĞDEM; Demir, H. İbrahim; EDİGER, DANE; YILMAZ, EMEL; ORAL, HALUK BARBAROS; AKALIN, EMİN HALİS; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Diş Hekimliği Fakültesi/Temel Bilimler Bölümü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Klinik Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; 0000-0001-7625-9148; 0000-0001-5334-7911; 0000-0001-8850-0269; 0000-0001-7288-3250; 0000-0002-5956-8755; 0000-0002-8856-7356; 0000-0001-7585-7971; 0000-0002-2954-4293; 0000-0003-1785-3539; 0000-0003-0463-6818; 0000-0001-7530-1279; AAG-7381-2021; AAH-3843-2020; K-7285-2012; F-4657-2014; IZP-9398-2023; AAU-8952-2020; HKN-2347-2023; DWR-5356-2022; KBR-5535-2024; GYL-2038-2022; GPN-1473-2022; AAE-9142-2019; GDP-0005-2022Publication Flow cytometric analysis of T, B, and NK cells antigens in patients with mycosis fungoides(Hindawi, 2015-11-24) Yazıcı, Serkan; Başkan, Emel Bülbül; Budak, Ferah; Oral, Barbaros; Adım, Şaduman Balaban; Kalın, Zübeyde Ceylan; Özkaya, Güven; Aydoğan, Kenan; Sarıcaoğlu, Hayriye; Tunalı, Şükran; YAZİCİ, SERKAN; BÜLBÜL BAŞKAN, EMEL; BUDAK, FERAH; ORAL, HALUK BARBAROS; BALABAN ADIM, ŞADUMAN; Kalın, Zübeyde Ceylan; ÖZKAYA, GÜVEN; AYDOĞAN, KENAN; SARICAOĞLU, HAYRİYE; Tunalı, Şükran; Uludağ Üniversitesi/Tıp Fakültesi/Dermatoloji ve Zührevi Hastalıklar Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0001-6407-0962; 0000-0001-7625-9148; 0000-0003-0463-6818; 0000-0003-0297-846X; 0000-0002-0193-1128; IZP-9398-2023; AAH-2459-2021; AAH-1388-2021; A-4421-2016; F-4657-2014; AAH-6216-2021; K-7285-2012; EMN-0789-2022; CWV-8710-2022; DPU-8534-2022; GGH-7385-2022We retrospectively analyzed the clinicopathological correlation and prognostic value of cell surface antigens expressed by peripheral blood mononuclear cells in patients with mycosis fungoides (MF). 121 consecutive MF patients were included in this study. All patients had peripheral blood flow cytometry as part of their first visit. TNMB and histopathological staging of the cases were retrospectively performed in accordance with International Society forCutaneous Lymphomas/European Organization of Research and Treatment of Cancer (ISCL/EORTC) criteria at the time of flow cytometry sampling. To determine prognostic value of cell surface antigens, cases were divided into two groups as stable and progressive disease. 17 flow cytometric analyses of 17 parapsoriasis (PP) and 11 analyses of 11 benign erythrodermic patients were included as control groups. Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3(+), CD4(+), CD8(+), TCR alpha beta(+), TCR gamma delta(+), CD7(+), CD4(+) CD7(+), CD4(+) CD7(-), and CD71(+)), B cells (HLA-DR+, CD19(+), and HLA-DR(+)CD19(+)), NKT cells (CD3(+)CD16(+)CD56(+)), and NK cells (CD3(-)CD16(+)CD56(+)). The mean value of all cell surface antigens was not statistically significant between parapsoriasis and MF groups. Along with an increase in cases of MF stage statistically significant difference was found between the mean values of cell surface antigens. Flow cytometric analysis of peripheral blood cell surface antigens in patients with mycosis fungoides may contribute to predicting disease stage and progression.Publication Impact of Storage Period on CD4+/CD8+ T Lymphocyte Ratio in Erythrocyte Suspensions(Turkish Soc Immunology, 2020-01-01) Bal, Salih Haldun; BAL, SALİH HALDUN; Kumaş, Levent Tufan; Heper, Yasemin; HEPER, YASEMİN; Budak, Ferah; BUDAK, FERAH; Göral, Guher; Can, Fatma Ezgi; Oral, Haluk Barbaros; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/BioistatistikAnabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; 0000-0001-7625-9148; 0000-0003-0463-6818; JSL-7718-2023; HJY-9001-2023; K-7285-2012; F-4657-2014; AAH-6506-2021; IZP-9398-2023Introduction: Some immunologic changes in the recipient derived by allogeneic blood transfusion (ABT) are called Transfusion Related Immune Modulation (TRIM). Despite the exact mechanisms of these changes are not known, it is thought that ABT causes a decrease of CD4/CD8 ratio in the recipient. This study aimed to determine the CD4/CD8 ratio in stored erythrocyte suspensions (ES) and to obtain new information about TRIM mechanisms.Materials and Methods: Whole blood components used in our study were collected from 10 healthy volunteers. ES' obtained from whole blood were divided into three equal aliquots. Test samples which were related to 0th, 21st and 42nd storage days were prepared from these aliquots. CD3, CD4 and CD8 surface markers in peripheral blood mononuclear cells were investigated with flow-cytometer in these test samples.Results: Our data were evaluated according to storage days. Decrease of CD3 (p=0,001), CD4 (p<0,001), CD8 (p=0,012) expressing mononuclear blood cells and helper T cells in 21st-day samples, CD4 (p=0,035) and CD8 (p=0,017) expressing cells in 42nd-day samples compared to Day 0 samples and increase of CD3 (p=0,027) expressing cells in 42nd-day samples compared to 21st-day samples were found statistically significant.Conclusion: In our study, we did not find any significant change in the ratio of CD4(+)/CD8(+) cells in ES. Detailed studies can help us to obtain more comprehensive knowledge on this field.Publication Investigation of changes in exosomes profile during storage period of erythrocyte suspensions(Springer India, 2020-08-11) Pashazadeh, Mehrdad; Oral, Haluk Barbaros; Budak, Ferah; Pashazadeh, Mehrdad; ORAL, HALUK BARBAROS; BUDAK, FERAH; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi İmmünoloji Anabilim Dalı.; 0000-0001-9103-6276; 0000-0001-7625-9148; IZP-9398-2023; F-4657-2014; DZE-7532-2022; FQR-1753-2022This study aimed to investigate the relationship of exosomes in erythrocyte suspension (ES) with leukoreduction and storage period. In this study, we expected to obtain new information about the immunomodulatory effect of allogeneic blood transfusion. Whole blood from healthy donors (10) were transferred to two separate blood bags by an equal amount converted into ES. One of the bags was passed through the leukocyte filter to obtain reduced leukocyte and non-reduced ESs and divided into four equal blood bags. Flow cytometry was used to identify and quantify exosomes in the samples on the 0th, 14th, 28th, and 42nd days at 4 degrees C. The exosomes were first coated with anti-CD9 antibodies with carboxylates beads and exosome volumes were determined by BCA Protein Assay to determine the amount of exosome-containing samples to be bound by beads and investigated by flow cytometry. Exosomes which were derived from Th, NK, NK-T cells were not detected in our study. There were no significant differences in exosome profiles between NL-ES and LR-ES groups. Statistically significant results (in comparison with the 0th day) were found as below. Exosomes that were derived from; T lymphocytes, decrease in LR-ES groups at 42nd-day samples (P < 0.05). Tc lymphocytes, decrease in NL-ES groups at 14th day and 42nd-day samples (P < 0.05). B lymphocytes, a decrease in NL-ES groups at the sample of 28th and 42nd-days (P < 0.05). MDSC and G-MDSC, decrease in both NL-ES groups and LR-ES groups at the sample of 14th, 28th, and 42nd-days (P < 0.05). Our results suggest that exosomes in erythrocyte suspensions are not affected by the leukoreduction procedure. Exosomes which can freely pass from leukocyte filters may be the main cause of the insufficiency of leukoreduction to prevent TRIM.Publication Mannose-binding lectin gene polymorphism and chronic hepatitis B infection in children(Wolters Kluwer Medknow Publications, 2015-03-01) Erdemir, Gülin; Özkan, Tanju B.; Özgür, Taner; Budak, Ferah; Kılıç, Sara S.; Önay, Huseyin; Erdemir, Gulin; Özkan, Tanju Başarır; ÖZGÜR, TANER; BUDAK, FERAH; KILIÇ GÜLTEKİN, SARA ŞEBNEM; Uludağ Üniversite/Tıp Fakültesi/Gastroenteroloji, Hepatoloji ve Beslenme Anabilim Dalı.; Uludağ Üniversite/Tıp Fakültesi/Pediatrik Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversite/Tıp Fakültesi/Pediatrik İmmünoloji Anabilim Dalı.; 0000-0002-9726-8219; 0000-0001-5740-9729; 0000-0001-7625-9148; 0000-0001-8571-2581; IZP-9398-2023; AAG-8381-2021; F-4657-2014; AAH-1658-2021; DTY-4609-2022Background/Aims: Mannose-binding lectin (MBL) is a member of innate immune system that activates complement system through lectin pathway. MBL deficiency is associated with susceptibility to infectious diseases. In this study, the relation between MBL gene polymorphism and chronic hepatitis B infection in children is evaluated. Patients and Methods: The study included 67 children with chronic hepatitis B and 99 healthy controls. The hepatitis B patients were divided into immuntolerant, chronic inactive, and treatment groups according to their laboratory findings. MBL gene codon 52, 54, and 57 polymorphisms were studied with polymerase chain reaction in all patients and controls. The associations of MBL gene polymorphism with clinical, laboratory, and histopathologic findings were evaluated. Results: Homozygous codon 54 polymorphism of MBL was found significantly higher in chronic hepatitis B patients than controls. Rate of the polymorphism was similar in all groups and, responsive and nonresponsive patients in the treatment group. Conclusions: The hepatitis B patients who are homozygous for codon 54 of MBL are prone to develop chronic infection. Longitudinal studies with larger groups are needed.Publication Microrna expression pattern modulates temozolomide response in gbm tumors with cancer stem cells(Springer/plenum Publishers, 2014-07-01) Preusser, Matthias; Berghoff, Anna Sophie; Ricken, Gerda; Tezcan, Gülçin; TEZCAN, GÜLÇİN; EGELİ, ÜNAL; Bekar, Ahmet; BEKAR, AHMET; KOCAELİ, HASAN; TUNCA, BERRİN; Tunca, Berrin; Çeçener, Gülşah; ÇEÇENER, GÜLŞAH; Budak, Ferah; BUDAK, FERAH; TAŞKAPILIOĞLU, MEVLÜT ÖZGÜR; Taşkapılıoğlu, Mevlüt Özgür; Tolunay, Şahsine; TOLUNAY, ŞAHSİNE; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Medikal Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Nöroşurji Anabilim Dalı.; 0000-0002-5956-8755; 0000-0002-1619-6680; 0000-0001-7904-883X; 0000-0002-3820-424X; 0000-0003-0388-7965; 0000-0001-7625-9148; 0000-0001-5472-9065; F-4657-2014; IZP-9398-2023; AAH-3843-2020; ABB-8161-2020; AAI-1612-2021; ABX-9081-2022; ABI-6078-2020; AAH-1420-2021; AAW-5254-2020; F-8554-2017; AAP-9988-2020Temozolomide (TMZ) is widely used to treat glioblastoma multiforme (GBM). Although the MGMT gene methylation status is postulated to correlate with TMZ response, some patients with a methylated MGMT gene still do not benefit from TMZ therapy. Cancer stem cells (CSCs) may be one of the causes of therapeutic resistance, but the molecular mechanism underlying this resistance is unclear. microRNA (miRNA) deregulation has been recognized as another chemoresistance modulating mechanism. Thus, we aimed to evaluate the miRNA expression patterns associated with chemoresistance that is dependent on the CSC status in GBM tumors to identify therapeutic biomarkers. CSCs were identified in 5 of 20 patients' tumor tissues using magnetic separation. CSC (+) tumors displayed a significant induction of CpG island methylation in the MGMT gene promoter (p = 0.009). Using real-time reverse transcription polymerase chain reaction (qRT-PCR), 9 miRNAs related to GBM (mir-181b, miR-153, miR-137, miR-145, miR-10a, miR-10b, let-7d, miR-9, and miR-455-3p), which are associated with cell cycle and invasion was analyzed in tumor samples. Low miR-181b and high miR-455-3p expression levels were detected (p = 0.053, p = 0.004; respectively) in CSC (+) tumors. Analysis revealed a significant correlation between miR-455-3p expression and Smad2 protein levels as analyzed by immunohistochemistry in CSC (+) tumors (p = 0.002). Thus, miR-455-3p may be involved in TMZ resistance in MGMT methylated CSC (+) GBM patients. Further studies and evaluations are required, but this miRNA may provide novel therapeutic molecular targets for GBM treatment and new directions for the development of anticancer drugs.Publication Olea europaea leaf extract improves the treatment response of GBM stem cells by modulating miRNA expression(E-century Publishing Corp, 2014-01-01) Tezcan, Gülçin; Tunca, Berrin; Bekar, Ahmet; Budak, Ferah; Şahin, Saliha; Çeçener, Gülşah; Egeli, Ünal; Taşkapılıoğlu, Mevlut Özgür; Kocaeli, Hasan; Tolunay, Şahsine; Malyer, Hulusi; Demir, Cevdet; Tümen, Gülendam; TEZCAN, GÜLÇİN; TUNCA, BERRİN; BEKAR, AHMET; BUDAK, FERAH; ŞAHİN, SALİHA; ÇEÇENER, GÜLŞAH; EGELİ, ÜNAL; TAŞKAPILIOĞLU, MEVLÜT ÖZGÜR; KOCAELİ, HASAN; TOLUNAY, ŞAHSİNE; MALYER, HULUSİ; DEMİR, CEVDET; Tümen, Gülendam; Uludağ Üniversitesi/Tıp Fakültesi/Beyin Cerrahi Kliniği; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Patholoji Bölümü; 0000-0002-5956-8755; 0000-0002-1619-6680; 0000-0001-7625-9148; 0000-0002-3820-424X; 0000-0001-7904-883X; 0000-0001-5472-9065; 0000-0002-9381-0410; ABA-2005-2020; F-8554-2017; ABX-9081-2022; AFR-1890-2022; F-4657-2014; AAH-3843-2020; AAI-1612-2021; ABI-6078-2020; IZP-9398-2023; AAW-5254-2020; AAP-9988-2020; AAH-2892-2021; ABB-8161-2020; AAH-1420-2021; ABI-6078-2020The stem-like cells of Glioblastoma multiforme (GBM) tumors (GSCs) are one of the important determinants of recurrence and drug resistance. The aims of the current study were to evaluate the anticancer effect of Olea europaea leaf extract (OLE) on GBM cell lines, the association between OLE and TMZ responses, and the effect of OLE and the OLE-TMZ combination in GSCs and to clarify the molecular mechanism of this effect on the expression of miRNAs related to cell death. The anti-proliferative activity of OLE and the effect of the OLE-TMZ combination were tested in the T98G, U-138MG and U-87MG GBM cell lines using WST-1 assay. The mechanism of cell death was analyzed with Annexin V/FITC and TUNEL assays. The effects of OLE on the expression levels of miR-181b, miR-153, miR-145 and miR-137 and potential mRNA targets were analyzed in GSCs using RT-qPCR. OLE exhibited anti-proliferative effects via apoptosis and necrosis in the GBM cell lines. In addition, OLE significantly induced the expression of miR-153, miR-145, and miR-137 and decreased the expression of the target genes of these miRNAs in GSCs (p < 0.05). OLE causes cell death in GBM cells with different TMZ responses, and this effect is synergistically increased when the cells are treated with a combination of OLE and TMZ. This is the first study to indicate that OLE may interfere with the pluripotency of GSCs by modulating miRNA expression. Further studies are required, but we suggest that OLE may have a potential for advanced therapeutic cancer drug studies in GBM.Publication Olea europaea leaf extract improves the treatment response of GBM stem cells by modulating miRNA expression(E-century Publishing Corp, 2014-01-01) Tümen, Gülendam; TUNCA, BERRİN; TEZCAN, GÜLÇİN; Tunca, Berrin; Bekar, Ahmet; BEKAR, AHMET; Budak, Ferah; BUDAK, FERAH; Egeli, Ünal; Taşkapılıoğlu, Mevlüt Özgür; Kocaeli, Hasan; Tolunay, Şahsine; Malyer, Hulusi; Demir, Cevdet; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-5956-8755; 0000-0002-1619-6680; 0000-0001-7625-9148; 0000-0002-3820-424X; 0000-0001-7904-883X; 0000-0001-5472-9065; 0000-0002-9381-0410; ABA-2005-2020; F-8554-2017; ABX-9081-2022; AFR-1890-2022; F-4657-2014; AAH-3843-2020; AAI-1612-2021; ABI-6078-2020; IZP-9398-2023; AAW-5254-2020; AAP-9988-2020; AAH-2892-2021; ABB-8161-2020; AAH-1420-2021The stem-like cells of Glioblastoma multiforme (GBM) tumors (GSCs) are one of the important determinants of recurrence and drug resistance. The aims of the current study were to evaluate the anticancer effect of Olea europaea leaf extract (OLE) on GBM cell lines, the association between OLE and TMZ responses, and the effect of OLE and the OLE-TMZ combination in GSCs and to clarify the molecular mechanism of this effect on the expression of miRNAs related to cell death. The anti-proliferative activity of OLE and the effect of the OLE-TMZ combination were tested in the T98G, U-138MG and U-87MG GBM cell lines using WST-1 assay. The mechanism of cell death was analyzed with Annexin V/FITC and TUNEL assays. The effects of OLE on the expression levels of miR-181b, miR-153, miR-145 and miR-137 and potential mRNA targets were analyzed in GSCs using RT-qPCR. OLE exhibited anti-proliferative effects via apoptosis and necrosis in the GBM cell lines. In addition, OLE significantly induced the expression of miR-153, miR-145, and miR-137 and decreased the expression of the target genes of these miRNAs in GSCs (p < 0.05). OLE causes cell death in GBM cells with different TMZ responses, and this effect is synergistically increased when the cells are treated with a combination of OLE and TMZ. This is the first study to indicate that OLE may interfere with the pluripotency of GSCs by modulating miRNA expression. Further studies are required, but we suggest that OLE may have a potential for advanced therapeutic cancer drug studies in GBM.Publication Predictors of drug survival of biologic therapies in psoriasis patients (vol 45, pg 813, 2020)(Taylor & Francis, 2020-08-31) Zorlu, Özge; Başkan, Emel Bülbül; BÜLBÜL BAŞKAN, EMEL; Yazıcı, Serkan; YAZİCİ, SERKAN; Sığırlı, Deniz; SIĞIRLI, DENİZ; Budak, Ferah; BUDAK, FERAH; Sarıcaoğlu, Hayriye; SARICAOĞLU, HAYRİYE; Aydoğan, Kenan; AYDOĞAN, KENAN; Cevhertas, Laçin; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim; 0000-0001-5555-130X; 0000-0001-6407-0962; 0000-0001-7625-9148; 0000-0002-0193-1128; 0000-0003-2287-3569; AAH-1388-2021; AAH-6216-2021; AAA-7472-2021; F-4657-2014; IZP-9398-2023; AAH-2459-2021Publication Prevalence of HLA-B*57 serotype associated with hypersensitivity reaction in HIV treatment in Turkish population(Wiley, 2021-08-01) Kızmaz, Muhammed Ali; Aymak, Figen; Oral, Haluk Barbaros; Budak, Ferah; Kızmaz, Muhammed Ali; Aymak, Figen; ORAL, HALUK BARBAROS; BUDAK, FERAH; Bursa Uludağm Üniversitesi/Tıp Fakültesi/İmminoloji Anabilim Dalı.; 0000-0001-5334-7911; 0000-0003-0463-6818; 0000-0001-7625-9148; F-4657-2014; K-7285-2012; IZP-9398-2023; HKN-2347-2023Publication Relationship of exosome profile with acute and chronic inflammation in brucellosis(Wiley, 2021-08-01) Şimşek, Abdurrahman; Kızmaz, Muhammed Ali; Ellergezen, Pınar Hız; Bal, Salih Haldün; Bozkurt, Tuğçe; Akalın, Emin Halis; Oral, Haluk Barbaros; Yılmaz, Özer; Budak, Ferah; ŞİMŞEK, ABDURRAHMAN; Kızmaz, Muhammed Ali; Ellergezen, Pınar Hız; BAL, SALİH HALDUN; Bozkurt, Tuğçe; AKALIN, EMİN HALİS; ORAL, HALUK BARBAROS; YILMAZ, ÖZER; BUDAK, FERAH; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Enfeksiyon ve Klinik Mikrobiyolojisi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-8850-0269; 0000-0001-5334-7911; 0000-0001-5144-091X; 0000-0001-7530-1279; 0000-0003-0463-6818; 0000-0001-7625-9148; IZP-9398-2023; K-7285-2012; AAG-7381-2021; HKN-2347-2023; AAU-8952-2020; F-4657-2014; CPH-1647-2022; KBR-5535-2024; KLE-8588-2024; EHB-5539-2022Publication Serum cancer from lung-6: Promising biomarker to differentiate cpfe from ipf(Mattioli 1885, 2022-01-01) Uzaslan, Esra; DEMİRDÖĞEN, EZGİ; UZASLAN, AYŞE ESRA; GÖREK DİLEKTAŞLI, ASLI; ÖZKAYA, GÜVEN; Dilektaşlı, Aslı Görek; Öztürk, Nilüfer Aylin Acet; Karadağ, Mehmet; KARADAĞ, MEHMET; ACET ÖZTÜRK, NİLÜFER AYLİN; YEŞİLBURSA, DİLEK; Yeşilbursa, Dilek; Budak, Ferah; BUDAK, FERAH; Öztürk, Alper; Ursavaş, Ahmet; COŞKUN, NECMİYE FUNDA; URSAVAŞ, AHMET; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kardiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmunoloji Anabilim Dalı.; 0000-0002-7400-9089; 0000-0001-7099-9647; 0000-0002-6375-1472; 0000-0001-7625-9148; 0000-0003-3604-8826; 0000-0003-0297-846X; 0000-0002-9027-1132; AAD-1271-2019; JPK-7012-2023; IZP-9398-2023; A-4421-2016; F-4657-2014; AAI-3169-2021; AAG-8744-2021Background: Combined pulmonary fibrosis and emphysema (CPFE) has been recognised as a phe-notype of pulmonary fibrosis. We aimed to compare serum surfactant protein-A (SP-A), surfactant protein-D (SP-D) and Krebs von den Lungen-6 (KL-6) levels, functional parameters, in CPFE and IPF (idiopathic pul-monary fibrosis) patients. Methods: Patients diagnosed with ???CPFE??? and ???IPF??? were consecutively included in 6 months as two groups. The patients with connective tissue diseases are excluded. Results: In this study, 47 patients (41 males, 6 females) with CPFE (n = 21) and IPF (n = 26) with a mean age of 70.12 ?? 8.75 were evaluated. CPFE patients were older, had more intense smoking history, had lower DLCO/VA, lower FVC, and worse six-minute walking distance than the IPF group (p=0.005, p=0.027, p=0.02, p<0.001, p=0.001, respec-tively). Serum KL-6 levels were higher in CPFE group compared to IPF group [264.70 U/ml (228.90-786) vs 233.60 (101.8-425.4), p<0.001]. Serum KL-6 levels of 245.4 U/ml and higher have 81% sensitivity and 73% specificity for the discrimination of CPFE from IPF. Conclusions: Our study has shown that serum KL-6 level is a promising biomarker to differentiate CPFE from IPF. In CPFE cases respiratory and functional parameters are worse than those of pure fibrosis cases.Publication Serum mannose binding level and gene polymorphism in down syndrome(Wiley, 2015-09-01) Kilic, Sara Şebnem; Budak, Ferah; KILIÇ GÜLTEKİN, SARA ŞEBNEM; BUDAK, FERAH; Uludağ Üniversitesi/Tıp Fakültesi/Pediatri Bölümü; 0000-0001-8571-2581; 0000-0001-7625-9148; AAH-1658-2021; IZP-9398-2023; F-4657-2014Publication The age-dependent role of Th22, Tc22, and Tc17 cells in the severity of pneumonia in COVID-19 immunopathogenesis(Wiley, 2021-08) Şimşek, Abdurrahman; Çağan, Eren; Kızmaz, Muhammed Ali; Dombaz, Fatma; Tezcan, Gülçin; Asan, Ali; Demir, H. İbrahim; Bal, S. Haldun; Ermiş, Diğdem Yoyen; Demirdöğen, Ezgi; Heper, Yasemin; Akalın, E. Halis; Oral, Haluk Barbaros; Budak, Ferah; ŞİMŞEK, ABDURRAHMAN; TEZCAN, GÜLÇİN; BAL, SALİH HALDUN; DEMİRDÖĞEN, EZGİ; BUDAK, FERAH; HEPER, YASEMİN; AKALIN, EMİN HALİS; Kızmaz, Muhammed Ali; Dombaz, Fatma; YÖYEN ERMİŞ, DİĞDEM; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Tıp Fakültesi; 0000-0001-8850-0269; 0000-0001-5334-7911; 0000-0001-7288-3250; 0000-0002-5956-8755; 0000-0002-7400-9089; 0000-0001-7625-9148; AAG-7381-2021; HKN-2347-2023; DWR-5356-2022; KBR-5535-2024; GYL-2038-2022; AAH-9812-2021; CTY-9474-2022; AAU-8952-2020; IZP-9398-2023; K-7285-2012; AAH-3843-2020