Browsing by Author "Ceron, Jose"
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Item Changes in serum proteins after endotoxin administration in healthy and choline-treated calves(BMC, 2016-09-10) İnan, Oya Eralp; Baykal, Ahmet T.; Hacariz, O.; Hatipoğlu, I.; Tvarijonaviciute, Asta; Ceron, Jose; Ulus, İsmail Hakkı; Yılmaz, Zeki; Cansev, Mehmet; Kocatürk, Meriç; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Eczacılık Anabilim Dalı.; 0000-0001-9836-0749; 0000-0003-2918-5064; M-9071-2019; V-5578-2017; 35944810500; 36437200800; 8872816100Background: This study aimed to investigate the possible serum protein changes after endotoxin administration in healthy and choline-treated calves using proteomics. These results are expected to contribute to the understanding of the pathophysiological mechanisms of endotoxemia and the beneficial effect of choline administration in this clinical situation. Methods: Healthy-calves (n = 20) were divided into 4 groups: Control, Choline treated (C), Lipopolysaccharide administered (LPS), and LPS + C. Control calves received 0.9 % NaCl injection. Calves in C and LPS + C groups received choline chloride (1 mg/kg/iv). Endotoxin (LPS) was injected (2 mu g/kg/iv) to the calves in LPS and LPS + C groups. Serum samples were collected before and after the treatments. Differentially expressed proteins (> 1.5 fold-change relative to controls) were identified by LC-MS/MS. Results: After LPS administration, 14 proteins increased, and 13 proteins decreased within 48 h as compared to controls. In the LPS group, there were significant increases in serum levels of ragulator complex protein (189-fold) and galectin-3-binding protein (10-fold), but transcription factor MafF and corticosteroid binding globulin were down regulated (>= 5 fold). As compared with the LPS group, in LPS + C group, fibrinogen gamma-B-chain and antithrombin were up-regulated, while hemopexin and histone H4 were down-regulated. Choline treatment attenuated actin alpha cardiac muscle-1 overexpression after LPS. Conclusions: LPS administration produces changes in serum proteins associated with lipid metabolism, immune and inflammatory response, protein binding/transport, cell adhesion, venous thrombosis, cardiac contractility and blood coagulation. The administration of choline is associated with changes in proteins which can be related with its beneficial effect in this clinical situation.Item Tei index (myocardial performance index) and cardiac biomarkers in dogs with parvoviral enteritis(Elsevier, 2012-02) Martinez, Silvia; Tvarijonaviciute, Asta; Ceron, Jose; Kocatürk, Meriç; Eralp, Oya; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0002-4242-8609; 0000-0001-9836-0749; AAG-2943-2020; 36437200800; 24472964600; 35944810500Tei index (myocardial performance) and cardiac biomarkers were evaluated in dogs with parvoviral enteritis (PVE). Tei index was calculated as isovolumic contraction time plus isovolumic relaxation time divided by ejection time. Myocardial and skeletal muscle damages were assessed by serum levels of cardiac troponin I (cTnI), creatine (phospho) kinase, lactate dehydrogenase and aspartate aminotransferase. Serum magnesium level was also determined. According to treatment response, dogs were divided into the survivor (n = 20) and non-survivor groups (n = 23). Seven healthy dogs served as controls. The mean value of the Tei index was higher in non-survivors, compared with survivors (p < 0.02) and healthy controls (p < 0.01). Serum level of cTnI in non-survivors was higher than that of survivors and controls (p < 0.05). Tei index showed the highest sensitivity and specificity to predict mortality. The findings of an elevated Tei index and an increase in serum cTnI are factors associated with a poor prognosis in cases of canine parvovirosis.