Browsing by Author "Ceron, Jose Joaquin"
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Item Changes in serum proteins in dogs with Ehrlichia canis infection(Elsevier, 2017-10-13) Escribano, Damian; Martinez, Silvia Subiela; Ceron, Jose Joaquin; Tvarijonaviciute, Asta; Cihan, Hüseyin; Levent, Pınar; Kocatürk, Meriç; Aytuğ, Nilüfer; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0001-9836-0749; ABH-3742-2020; V-5578-2017; 6602381681; 56690616700; 36437200800; 6505835923; 35944810500The aim of this study was the identification of proteins differentially represented in the serum proteome of seropositive dogs with (Group 1) and without (Group 2) clinical-pathologic signs consistent with ehrlichiosis compared to healthy control dogs. Serum samples were collected from 20 dogs of various breeds with naturally occurring ehrlichiosis (10 dogs belonged to Group 1 and 10 to Group 2) and 10 healthy dogs. Two-dimensional electrophoresis (2DE) of pooled serum for each of the group of dogs were run in triplicate. 2D image analysis showed 39 spots differently expressed between Group 1 and Group 2 compared with healthy ones. Mass spectrometry analysis allowed identification of 6 proteins: albumin, haptoglobin (Hp), alpha-l-antitrypsin (AAT), Retinol Binding Protein 4 (RBP-4), alpha-l-acid glycoprotein (AGP) and vitamin D-binding protein (VDBP). When a confirmatory study was performed for albumin, Hp, AAT and RBP-4 by using different assays, significant differences (P < 0.05) between diseased and healthy groups were observed. It can be concluded that there are significant changes in the serum proteome of dogs with ehrlichiosis with modifications in proteins related with the acute phase response such as Hp, albumin and AGP, with vitamin A transport such as RBP-4, with inhibitors of serine proteases and anti-inflammatory proteins such as AAT, and vitamin D metabolism and actin scavengers such as VDBP.Item Changes of inflammatory and oxidative stress biomarkers in dogs with different stages of heart failure(BMC, 2020-11-10) Rubio, Camila Peres; Tanaka, Ryou; Koch, Jorgen; Ceron, Jose Joaquin; Sarıl, Ahmet; Kocatürk, Meriç; Yılmaz, Zeki; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; 0000-0002-2849-1222; DQX-5263-2022; GGO-7900-2022; 57202390224; 36437200800; 35944810500BackgroundHeart failure (HF) is associated with changes in inflammatory and oxidative stress biomarkers. This study aimed to evaluate the changes of a panel of inflammatory and oxidative stress biomarkers in dogs with different stages of HF and its relation with the severity of the disease and echocardiographic changes. A total of 29 dogs with HF as a result of myxomatous mitral valve degeneration or dilated cardiomyopathy were included and classified as stage-A (healthy), B (asymptomatic dogs), C (symptomatic dogs) and D (dogs with end-stage HF) according to the ACVIM staging system. In these dogs an ecnhocardiographic examination was performed and cytokines, and inflammatory and oxidative stress markers were evaluated in serum.ResultsKC-like was significantly increased in dogs of stage-C (P<0.01) and -D (P<0.05) compared with stage-A and -B. Stage-D dogs showed significantly higher serum CRP and Hp (P<0.05) but lower serum antioxidant capacity (PON1, TEAC, CUPRAC, and thiol) compared to stage-A and -B (P<0.05). After the treatment, serum levels of CRP, Hp and KC-like decreased and serum antioxidant levels increased compared to their pre-treatment values. Left ventricular dimension and LA/Ao ratio correlated positively with CRP, MCP-1, and KC-like but negatively with PON1, GM-CSF, IL-7 and antioxidant biomarkers (P<0.01).ConclusionOur results showed that dogs with advanced HF show increases in positive acute-phase proteins and selected inflammatory cytokines such as KC-like, and decreases in antioxidant biomarkers, indicating that inflammation and oxidative stress act as collaborative partners in the pathogenesis of HF. Some of these biomarkers of inflammation and oxidative stress could have the potential to be biomarkers to monitor the severity of the disease and the effect of treatment.Publication Choline or cdp-choline restores hypotension and improves myocardial and respiratory functions in dogs with experimentally-induced endotoxic shock(Elsevier, 2021-10-27) Ozarda, Yesim; Ceron, Jose Joaquin; Buturak, Ali; Ulus, Ismail H.; Kocaturk, Meric; KOCATÜRK, MERİÇ; Yilmaz, Zeki; YILMAZ, ZEKİ; Cansev, Mehmet; CANSEV, MEHMET; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-2849-1222; 0000-0001-9836-0749; 0000-0003-2918-5064; 0000-0002-8654-1793; H-9043-2015; D-5340-2015; V-5578-2017; A-9637-2008Endotoxin shock is associated with severe impairments in cardiovascular and respiratory functions. We showed previously that choline or cytidine-5 '-diphosphocholine (CDP-choline) provides beneficial effects in experimental endotoxin shock in dogs. The objective of the present study was to determine the effects of choline or CDPcholine on endotoxin-induced cardiovascular and respiratory dysfunctions. Dogs were treated intravenously (i.v.) with saline or endotoxin (LPS, 0.1 mg/kg) 5 min before i.v. infusion of saline, choline (20 mg/kg) or CDP-choline (70 mg/kg). Blood pressure, cardiac rate, myocardial and left ventricular functions, respiratory rate, blood gases, serum electrolytes and cardiac injury markers were determined before and at 0.5-48 h after endotoxin. Plasma tumor necrosis factor alpha (TNF-alpha), high mobility group box-1 (HMGB1), catecholamine and nitric oxide (NO) levels were measured 2 h and 24 h after the treatments. Endotoxin caused immediate and sustained reductions in blood pressure, cardiac output, pO2 and pH; changes in left ventricular functions, structure and volume parameters; and elevations in heart rate, respiratory rate, pCO2 and serum electrolytes (Na, K, Cl, Ca and P). Endotoxin also resulted in elevations in blood levels of cardiac injury markers, TNF-alpha, HMGB1, catecholamine and NO. In choline- or CDP-choline-treated dogs, all endotoxin effects were much smaller in magnitude and shorter in duration than observed values in controls. These data show that treatment with choline or CDP-choline improves functions of cardiovascular and respiratory systems in experimental endotoxemia and suggest that they may be useful in treatment of endotoxin shock in clinical setting.Item Platelet proteome changes in dogs with congestive heart failure(BMC, 2020-11-30) Akgün, Emel; Çevik, Özge; Baykal, Ahmet Tarık; Tanaka, Ryou; Ceron, Jose Joaquin; Levent, Pınar; Kocatürk, Meriç; Sarıl, Ahmet; Yılmaz, Zeki; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; DDR-1481-2022; T-4585-2019; A-9637-2008; 56690616700; 36437200800; 57202390224; 35944810500Background: Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in congestive heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n = 10) and dogs with acute CHF due to myxomatous mitral valve disease (MMVD, n = 10) were used. Acute CHF was defined based on the clinical (increased respiratory rate or difficulty breathing) and radiographic findings of pulmonary edema. Dogs Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris. Results: Totally 104 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in acute CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in acute CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses. Conclusion: This study describes by first time the changes in the protein composition in platelets of dogs with acute CHF due to MMVD. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF caused by MMVD and could be a tool for further investigations about the prevention and treatment of this disease.Item Prognostic value of serum acute-phase proteins in dogs with parvoviral enteritis(Wiley, 2010-09) Martinez, Silvia; Tvarijonaviciute, Asta; Ceron, Jose Joaquin; Kocatürk, Meriç; Eralp, Oya; Yılmaz, Zeki; Uludağ Üniversitesi/Veterinerlik Fakültesi/Klinik Bilimler Bölümü.; 0000-0001-9836-0749; 36437200800; 24472964600; 35944810500OBJECTIVE: To evaluate the acute-phase protein response in dogs with parvoviral enteritis as predictor of the clinical outcome. Methods: Canine parvovirus infection was diagnosed based on the compatible clinical findings and confirmed by the canine parvovirus antigen test in 43 dogs of less than six months of age. Blood samples for complete blood cell count and acute-phase proteins (C-reactive protein, haptoglobin, ceruloplasmin and albumin) were collected before treatment. Twenty-three dogs died during or after treatment (non-survival) and the rest recovered (survival). Five healthy dogs were enrolled as control. Results: Serum C-reactive protein, ceruloplasmin and haptoglobin levels in dogs with parvoviral enteritis were higher (P < 0.001, P < 0.01 and P < 0.001, respectively), but serum albumin was lower (P < 0.001) than those in controls. Mean C-reactive protein and ceruloplasmin values in non-survival were higher (P < 0.01) than those for survival dogs. C-reactive protein was found to be superior to ceruloplasmin, haptoglobin and albumin for distinguishing survival from non-survival dogs. Values higher than 92.4 mg/l for C-reactive protein had a sensitivity of 91% to predict mortality. Clinical Significance: The magnitude of the increase in serum acute-phase proteins in dogs with parvoviral enteritis could be a useful indicator of the prognosis of the disease. In acute-phase proteins, C-reactive protein is a potent predictor of mortality in dogs with parvoviral enteritis.