Browsing by Author "Demir, Kamber"
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Item Administration time of misoprostol affects fertility rate in artificially inseminated Kivircik ewes with frozen-thawed ram semen(Brazilian Coll Animal Reproduction, 2018-05-15) Demir, Kamber; Üstüner, Hakan; Üstüner, Burcu; Toker, M. Berk; Alçay, Selim; Sağırkaya, Hakan; Nur, Zekeriya; Uludağ Üniversitesi/Veteriner Fakültesi/Zootekni Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı.; 0000-0002-1438-221X; 0000-0003-4033-9749; 0000-0002-4561-6189; 0000-0002-4341-5842; AAH-2635-2021; A-2794-2014; D-2411-2019; AAG-9127-2021; AAH-8821-2021; AAG-7238-2021; AAH-8751-2019; 16065222700; 18937724600; 56480349200; 56099810300; 6602400461; 6508060684The aim of this study was to determine the effects of the administration time of misoprostol (11 h (Miso11) and 6 h (Miso6) before artificial insemination) on fertility rates in Kivircik ewes (control: n = 41, Miso11: n = 32 and Miso6: n = 33) during breeding season. Artificial insemination (AI) was performed 48 h after sponge removal using frozen-thawed semen (150 million sperm per dose in 0.25 ml straws). Estrus synchronization parameters (onset and duration) and lambing rate were evaluated. No significant difference was observed among groups for the estrus onset and duration hours (P > 0.05). The lambing rates in the control, Miso11 and Miso6 groups were 39.0, 62.5 and 54.5%, respectively. There were significant differences among the control, Miso11 and Miso6 groups according to lambing rates (P < 0.05). In conclusion, misoprostol treatment significantly improved fertility in ewes when using frozen-thawed semen in AI. Administration of misoprostol 11 h before AI resulted in a higher lambing rate than that at 6 h before AI; therefore, treatment of misoprostol 11 h before AI can effectively be used.Item Reproductive performance of first cloned Anatolian Grey Cattle produced by frozen cells from National Animal Gene Bank(Elsevier, 2014-09) Arat, Sezen; Pabuçcuoğlu, Serhat; Alkan, Serhat; Demir, Kamber; Arıcı, Ramazan; Kılıçaslan, Ragıp; Sağırkaya, Hakan; Nak, Yavuz; Alçay, Selim; Üstüner, Burcu; Nak, Deniz; Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.; AAH-8821-2021; AAH-5494-2021Item Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle(Csiro Publishing, 2011) Arat, Sezen; Çaputçu, Arzu Taş; Akkoç, Tolga; Pabuccuoğlu, Serhat; Cirit, Umut; Koban, Evren; Bağış, Haydar; Demir, Kamber; Şenünver, Adem; Kılıçaslan, Ragip; Çetinkaya, Gaye; Denizci, Melis; Aslan, Özgür; Sağırkaya, Hakan; Nak, Yavuz; Nak, Deniz; Tuna, Bilginer; Uludağ Üniversitesi/Veterinerlik Fakültesi/Üreme ve Suni Tohumlama Bölümü.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; AAH-5494-2021; 6602400461; 8615464000; 9280090000The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.