Browsing by Author "Escribano, Damián"
Now showing 1 - 2 of 2
- Results Per Page
- Sort Options
Item Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study(Elsevier, 2018-09-18) Franco-Martínez, Lorena; Tvarijonaviciute, Asta; Horvatić, Anita; Guillemin, Nicolas; Cerón, José Joaquín; Escribano, Damián; Eckersall, David; Lamy, Elsa; Martínez-Subiela, Silvia; Mrljak, Vladimir; Kocatürk, Meriç; Yılmaz, Zeki; Uludağ Üniversitesi/Veterinerlik Fakültesi/Dahiliye Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; 36437200800; 35944810500The present study evaluated the changes in salivary proteome in parvoviral enteritis (PVE) in dogs through a high-throughput quantitative proteomic analysis. Saliva samples from healthy dogs and dogs with severe parvovirosis that survived or perished due to the disease were analysed and compared by Tandem Mass Tags (TMT) analysis. Proteomic analysis quantified 1516 peptides, and 287 (corresponding to 190 proteins) showed significantly different abundances between studied groups. Ten proteins were observed to change significantly between dogs that survived or perished due to PVE. Bioinformatics' analysis revealed that saliva reflects the involvement of different pathways in PVE such as catalytic activity and binding, and indicates antimicrobial humoral response as a pathway with a major role in the development of the disease. These results indicate that saliva proteins reflect physiopathological changes that occur in PVE and could be a potential source of biomarkers for this disease.Item Serum antioxidant capacity and oxidative damage in clinical and subclinical canine ehrlichiosis(Elsevier, 2017-06-10) Rubio, Camila Peres; Martínez, Silvia Subiela; Hernández, Josefa Ruiz; Tvarijonaviciute, Asta; Escribano, Damián; Ceron, Jose Joaquín; Yılmaz, Zeki; Kocatürk, Meriç; Yalçın, Ebru; Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; 35944810500; 36437200800; 36778554000The objective of this study was to evaluate and compare the antioxidant response and the products of oxidative damage analysed by various assays in clinical and subclinical canine monocytic ehrlichiosis (CME). For this purpose, four assays to measure the total serum antioxidant capacity (TAC), such as the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC) using acidic medium (TEAC(A)), and the TEAC using the horseradish peroxidase (TEAC(H)) were used. In addition, the serum thiol concentrations were analysed. Reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and ferrous oxidation-xylenol orange (FOX) were measured to determine the concentrations of free radical and the products of oxidative damage as result of the disease. All antioxidant markers were significantly lower in the dogs on clinical ehrlichiosis when compared with healthy dogs; however only the CUPRAC, FRAP and thiol were significantly lower in subclinical CME compared with healthy dogs. TBARS and FOX showed no significant differences between dogs with CME and healthy dogs; however, a significant increased ROS concentration was observed in dogs with clinical and subclinical CME when compared with healthy dogs. Results showed that in CME there is a state of oxidative stress with significant changes in markers of antioxidant defence and in concentrations of free radicals. However, the detection of these changes would depend of the assay used.