Browsing by Author "Guzman, Josune"
Now showing 1 - 3 of 3
- Results Per Page
- Sort Options
Item Bronchoalveolar lavage in drug-induced lung disease(W B Saunders Co-Elsevier, 2004-03) Costabel, Ulrich; Guzman, Josune; Uzaslan, Esra; Uludağ Üniversitesi/Tıp Fakültesi.This article reviews some technical aspects of bronchoalveolar lavage (BAL), gives an overview on the use of BAL in the differential diagnosis of interstitial lung disease, and concludes with a detailed description of the BAL findings in drug-induced lung disease.Item Cockade-like structures in alveolar macrophages in extrinsic allergic alveolitis(Karger, 2005) Guzman, Josune; Costabel, Ulrich; Uzaslan, Esra Kunt; Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; 8761653500Background and Objectives: In immunocytochemical preparations of bronchoalveolar lavage (BAL) cells from patients with extrinsic allergic alveolitis (EAA), we observed the presence of alveolar macrophages with cockade-like structures in their cytoplasm ( cockade+ alveolar macrophages). These cockade+ alveolar macrophages may reflect a subpopulation of alveolar macrophages which may show a different predominance in various interstitial lung diseases. In this study we aimed to compare the frequency of cockade+ alveolar macrophages in patients with EAA ( n = 14) with the results obtained in patients with sarcoidosis ( n = 11), idiopathic interstitial pneumonia ( IIP; n = 10) and control subjects ( n = 8). We also investigated the expression of the transferrin receptor CD71 on cockade+ alveolar macrophages. Methods: In BAL fluid, the total number of cells and differential counts were determined, and immunocytologic examinations of macrophages and lymphocytes were done using monoclonal antibodies. The percentage of cockade+ alveolar macrophages was determined by counting 300 macrophages in the CD20 field of an immunocytochemical slide. Results: The percentage of cockade+ alveolar macrophages was significantly higher in the EAA group ( 36 +/- 9%) compared to patients with sarcoidosis ( 12 +/- 5%) or IIP ( 11 +/- 10%) and control subjects ( 3 +/- 1%; p < 0.001). The proportion of CD71+ alveolar macrophages was significantly lower in EAA than in the other groups ( p < 0.01), and the CD71 antigen was expressed on a significantly lower proportion of cockade+ alveolar macrophages compared to cockade - alveolar macrophages in EAA ( p < 0.001). Conclusion: We conclude that cockade+ alveolar macrophages could play a role in the pathogenesis and differential diagnosis EAA.Item Reactive alveolar epithelium in chondroid hamartoma of the lung(Karger, 2005) Ebsen, Michael; Freudenberg, Nikolaus; Nakamura, Shinobu; Costabel, Ulrich; Guzman, Josune; Uzaslan, Esra; Uludağ Üniversitesi/Tıp Fakültesi/Göğüs Hastalıkları Anabilim Dalı.; 8761653500Objective To determine the morphologic characteristics of the nonciliated epithelium found in chondroid hamartoma of the lung. Study Design The morphologic characteristics and immunohistochemical reaction for surfactant protein A of the nonciliated epithelium, in chondroid hamartoma of the lung was studied by immunohistochemistry. Alveolar epithelium in normal lung tissue and lung tissue surrounding primary lung cancer or metastatic lung lesions was used as a control. Results In all cases, the nonciliated epithelium in chondroid hamartoma showed the morphologic criteria of hyperplastic alveolar type II cells and a very strong positive surfactant protein A reaction in the cytoplasm, when compared with alveolar epithelium of the normal lung. Similar hyperplastic type II cells were also found in the alveolar lung around metastatic or primary lung tumors. Conclusion These findings may indicate that the nonciliated cells found in chondroid hamartoma of the lung are hyperplastic type H cells. This suggests that the alveolar epithelium found in chondroid hamartoma of the lung is a secondary reaction around the hamartoma and not a primary component of the lesion.