Browsing by Author "Inci, Duygu"
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Item Cu (II) tyrosinate complexes containing methyl substituted phenanthrolines: synthesis, x-ray crystal structures, biomolecular interactions, antioxidant activity, ros generation and cytotoxicity(Wiley, 2019-01) Cosut, Bunyemin; Zorlu, Yunus; Inci, Duygu; Aydın, Rahmiye; Vatan, Ozgür; Hüriyet, Huzeyfe; Cinkılıç, Nilufer; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya, Anorganik Kimya Bölümü.; 0000-0002-0483-9642; 0000-0002-3595-6286; AAH-8936-2021; AAH-5296-2021; G-2201-2019; 55082306300; 56261495600; 16235098100; 57201093675; 26533892300In this paper, three new copper (II) complexes, [Cu(4-mphen)(tyr)(H2O)]ClO4 (1), [Cu(5-mphen)(tyr)(H2O)]ClO4 center dot 1.5H(2)O (2) and [Cu (tmphen)(tyr)(NO3)]0.5H(2)O (3) (4-mphen: 4-methyl-1,10-phenanthroline, 5-mphen: 5-methyl-1,10-phenanthroline, tmphen: 3,4,7,8-tetramethyl-1,10-phenanthroline and tyr: L-tyrosine), were synthesized and characterized using elemental analyses, FT-IR, ESI-MS, cyclic voltammetry and single-crystal X-ray diffraction. It was found that the complexes adopt a distorted five-coordinate square pyramidal geometry. The interaction of the three complexes with calf thymus DNA was also investigated using UV-visible absorption spectra, ethidium bromide and Hoechst 33258 displacement assay and thermal denaturation. The DNA cleavage activity of the complexes, monitored using gel electrophoresis, showed significant damage of the pUC19 plasmid DNA. Binding activity of bovine serum albumin (BSA) reveals that these complexes can strongly quench the fluorescence of BSA through a static quenching mechanism. The results suggested that interaction of the complexes with DNA occurred through a partial intercalation into the minor grooves of DNA. In addition, interaction of the complexes with bovine serum albumin quenched the fluorescence emission of the tryptophan residues of the protein binding constants and thermodynamic parameters were obtained from the fluorescence quenching experiments at different temperatures. Free radical scavenging activities of the complexes were determined by various in vitro assays such as 1,1-diphenyl-2-picryl-hydrazyl free radicals (DPPH) and H2O2 scavenging methods. In addition, the cytotoxicity of these complexes in vitro on tumor cell lines (Caco-2 and MCF-7) was examined by XTT and showed better antitumor effect on the tested cells. ROS (reactive oxygen species) and comet experiments are consistent with each other and these complexes lead to DNA damage via the production of ROS. The effect of the hydrophobic properties of the synthesized complexes on DNA and BSA binding activities were discussed.Publication Neocuproine copper(ii) complexes with bioactive ligands in aqueous solution(Maik Nauka/interperiodica/Springer, 2020-12-01) Inci, Duygu; Aydın, Rahmiye; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/ Kimya Bölümü.; 0000-0003-4944-0181; AAH-8936-2021A potentiometric titration technique has been used to determine protonation constants of neocuproine {(neo): 2,9-dimethyl-1,10-phenanthroline} and bioactive ligands {(N,O-donor chelator, N,O): glycine (gly), leucine (leu), methionine (met), phenylalanine (phe), tyrosine (tyr), tryptophan (trp), asparagines (asn), glutamine (gln), aspartic acid (asp), glutamic acid (glu)} and their binary and ternary stability constants with copper(II) ion were determined at the constant ionic strength 0.1 M KCl and at 298.15 K. The protonation constants of neo and bioactive ligands and their stability constants of the binary and ternary copper(II) complexes were computed from the potentiometric titration data using BEST program. Furthermore, SPE program was used to determine the distribution of the species formed in solution medium. The stability of the ternary copper(II) complexes are compared with those of the corresponding the stability of the binary copper(II) complexes with regard to Delta logK values.Item Water-soluble binary and ternary palladium(II) complexes containing amino acids and intercalating ligands: Synthesis, characterization, biomolecular interactions and cytotoxicities(Royal Sciety, 2019-03-28) Sahin, Onur; Inci, Duygu; Aydin, Rahmiye; Vatan, Ozgür; Cinkılıc, Nilüfer; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji/Genel Biyoloji Bölümü.; 0000-0003-4944-0181; 0000-0002-0483-9642; 0000-0002-7687-3284; 0000-0002-3595-6286; G-2201-2019; O-7508-2015; AAH-5296-2021; AAH-8936-2021; 55082306300; 56261495600; 16235098100; 26533892300In this study, six water soluble cationic binary (1-3) palladium() complexes of the type [PdA(2)](NO3)(2)(n - 1)H2O and ternary (4-6) palladium() complexes of the type [PdA(amino acid)](NO3)(n - 1)H2O, where A is 1,10-phenanthroline (phen) (1, 4, 6), 5-methyl-1,10-phenanthroline (5-mphen) (2, 5), and 3,4,7,8-tetramethyl-1,10-phenanthroline (tmphen) (3) and the amino acid is l-tyrosine (tyr) (4, 5) and glycine (gly) (6), have been synthesized and characterized using CHN elemental analyses, ATR-FT-MIR, H-1 NMR and C-13 NMR and single-crystal X-ray diffraction techniques. The interaction of the complexes with calf thymus DNA (CT-DNA) was investigated using electronic absorption spectral, ethidium bromide (EB) and Hoechst 33258 (HO) displacement assays and thermal denaturation. The pUC19 DNA cleavage activity of the complexes was investigated in the absence and presence of external agents by agarose gel electrophoresis. Quenching of the tryptophan and tyrosine residues of bovine serum albumin (BSA) by the complexes was found to be static. Furthermore, the in vitro cytotoxic effect of the complexes was examined on human tumor cell lines (Caco-2, A549, and MCF-7) and healthy cells (BEAS-2B). All the complexes exhibited different cytotoxic activities.