Browsing by Author "Ipek, Ahmet"

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Characterization of phytochemical and functional properties of endemic edible Allium tuncelianum and comparison with commonly cultivated garlic, onion and leek crops
(Springer, 2023-01-13) İPEK, MERYEM; ŞAHAN, YASEMİN; CANSEV, ASUMAN; Cansev, Asuman; İPEK, AHMET; Ipek, Ahmet; Altınseker Acun, Deniz Zahide; Bursa Uludağ Üniversitesi/Ziraat Fakültesi.; AAH-3233-2021; ABE-6748-2020; AAH-4255-2019; AAK-4655-2021
Edible Allium tuncelianum ([Kollman] Ozhatay, Matthew and Siraneci), endemic to Turkey, has received increased attention in recent years because of its appealing characteristics, such as garlic (A. sativum L.)-like odor and single-clove solid bulb structure with few peels, which could be advantageous in the food processing industry. The aims of this study were to determine chemical composition, phytochemicals (alliin, allicin, phenolics), antioxidant capacity, and bioaccessibility of total phenolic content and antioxidant capacity in A. tuncelianum and to compare with the values in commonly cultivated Allium species: garlic, onion (A. cepa L.), and leek (A. porrum L.) to reveal its nutritional and health beneficial properties. The chemical composition of A. tuncelianum was comparable with that of garlic, and its dietary fiber content was three times higher than that of garlic. Alliin and allicin organosulfur compounds, which give garlic its distinct flavor and odor, were detected in all analyzed A. tuncelianum genotypes although their values lower than the amounts detected in garlic genotypes. However, alliin and allicin were not detected in any onion and leek genotypes. A. tuncelianum genotypes had higher total phenolic contents with over 78 percent bioaccessibility rates than garlic, onion, and leek. The antioxidant capacity of A. tuncelianum was comparable to those of cultivated Allium species. Our results suggested that A. tuncelianum can be considered a promising new health-enhancing crop for human nutrition.(GRAPHICS)
Demonstration of linkage and development of the first low-density genetic map of garlic, based on AFLP markers
(Springer, 2005-01) Ipek, Meryem; Almquist, SG; Simon, Phillip W.; Ipek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.
Garlic (Allium sativum L.) is a long-cultivated, clonally propagated diploid plant (2n=2x=16). With routine seed production now underway, we used populations (MP1 and MP2) generated by self-pollination of unrelated plants to generate two low-density genetic maps of garlic, consisting of amplified fragment length polymorphism (AFLP) and gene-specific markers. We did not observe any two plants with identical marker patterns in either population, indicating that they were the result of amphimixis rather than apomixis. This is an important finding, since several Alliums are facultative apomicts. A total of 360 markers segregated in MP1 (12.8 AFLP markers per primer combination) and 321 markers segregated in MP2 (13.9 per primer combination) to indicate a fairly high level of genetic heterozygosity in the garlic nuclear genome. Of these markers, 15.3% in MP1 and 24.3% in MP2 had segregation ratios distorted from the expected 3: 1. Interestingly, 94.7% of those distorted segregations fit a 15: 1 segregation ratio for duplicated loci, suggesting extensive levels of duplication in the garlic genome and supporting similar observations for onion. The genetic map for the MP1 family with 216 markers spanned 1,166 cM of the garlic genome ( 5.4 cM average), while 143 markers of MP2 spanned 862 cM ( 6.0 cM average). Gene-specific markers for alliinase, chitinase, sucrose 1-fructosyltransferase (SST-1), and chalcone synthase (CHS) were mapped, demonstrating the immediate utility of the garlic genetic map. These two garlic families had relatively few segregating AFLP markers in common, which supports their relatively distant relationship based on diversity analysis. Of those markers that were conserved, linkages were also conserved.
Development and validation of new SSR markers from expressed regions in the garlic genome
(Univ Sao Paulo, 2015-01) Sahin, Nihan; Simon, Philipp W.; Ipek, Meryem; Ipek, Ahmet; Cansev, Asuman; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-0609-3442; 0000-0002-9136-3186; 0000-0002-3353-846X; AAH-3233-2021; AAK-4655-2021; AAH-4255-2019; 16031208900; 6603912487; 26326677200
Only a limited number of simple sequence repeat (SSR) markers is available for the genome of garlic (Allium sativum L.) despite the fact that SSR markers have become one of the most preferred DNA marker systems. To develop new SSR markers for the garlic genome, garlic expressed sequence tags (ESTs) at the publicly available GarlicEST database were screened for SSR motifs and a total of 132 SSR motifs were identified. Primer pairs were designed for 50 SSR motifs and 24 of these primer pairs were selected as SSR markers based on their consistent amplification patterns and polymorphisms. In addition, two SSR markers were developed from the sequences of garlic cDNA-AFLP fragments. The use of 26 EST-SSR markers for the assessment of genetic relationship was tested using 31 garlic genotypes. Twenty six EST-SSR markers amplified 130 polymorphic DNA fragments and the number of polymorphic alleles per SSR marker ranged from 2 to 13 with an average of 5 alleles. Observed heterozygosity and polymorphism information content (PIC) of the SSR markers were between 0.23 and 0.88, and 0.20 and 0.87, respectively. Twenty one out of the 31 garlic genotypes were analyzed in a previous study using AFLP markers and the garlic genotypes clustered together with AFLP markers were also grouped together with EST-SSR markers demonstrating high concordance between AFLP and EST-SSR marker systems and possible immediate application of EST-SSR markers for fingerprinting of garlic clones. EST-SSR markers could be used in genetic studies such as genetic mapping, association mapping, genetic diversity and comparison of the genomes of Allium species.