Browsing by Author "Joaquin Ceron, Jose"
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Item Serum apolipoprotein-A1 as a possible biomarker for monitoring treatment of canine leishmaniosis(Elsevier, 2016-10-10) Escribano, Damian; Tvarijonaviciute, Asta; Joaquin Ceron, Jose; Pardo-Marin, Luis; Torrecillas, Alejandro; Martinez-Subiela, Silvia; Kocatürk, Meriç; Yılmaz, Zeki; Uludağ Üniversitesi/Veteriner Fakültesi/Dahiliye Anabilim Dalı.; 0000-0001-9836-0749; V-5578-2017; 36437200800; 35944810500The aims of this study were: the identification of proteins differentially represented in the serum proteome of dogs with leishmaniosis after treatment and the verification of one selected protein as a possible biomarker for treatment monitoring. Serum samples from five dogs with leishmaniosis, before and after treatment were pooled into two groups and analysed using 2-dimensional electrophoresis followed by mass spectrometry analysis (MS). The MS analysis allowed the identification of 8 proteins differently expressed. APO-A1 was selected and an immunoturbidimetric assay was validated for its measurement in dogs. Significantly decreased concentrations of APO-A1 in dogs with leishmaniosis and a significant increase after a good response to the treatment were observed, suggesting that APO-A1 could be a potential biomarker of treatment monitoring with the advantages of an automated measurement.