Browsing by Author "KUMRAL, NABİ ALPER"

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A laboratory study of the acaricidal, repellent and oviposition deterrent effects of three botanical oils on Tetranychus urticae (Koch, 1836) (Acari: Tetranychidae)
(Ege Üniversitesi, 2020-04-25) Keskin, Gizem; Kumral, Nabi Alper; Kacar, Oya; Keskin, Gizem; KUMRAL, NABİ ALPER; KAÇAR, OYA; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Entomoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü.; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Tarla Bitkileri Bölümü.; 0000-0001-9442-483X; A-1388-2019; AAG-7449-2021; KGA-9390-2024
The biological activities of essential oil obtained from water distillation process of basil leaves [Ocimum basilicum L. (Lamiales: Lamiaceae)] cv Round Midnight and crude oil obtained from the cold-pressed process of chinaberry tree seeds [Melia azedarach L. (Sapindales: Meliaceae)] and a commercial neem oil product (Nimbecidine) [Azadirachta indica (A. Juss, 1830) (Sapindales: Meliaceae)] were assessed against two-spotted spider mites, Tetranychus urticae (Koch, 1836) (Atari: Tetranychidae) using a residual method on leaf disc under laboratory conditions at Bursa Uludag University during 2018-2019. The lethal concentrations (LC50 and LC90) of Nimbecidine, chinaberry and basil oils were estimated as 0.8 and 1.8 mg/L, 4.0 and 6.9%, 5.4 and 11.7%, respectively, 72 h after treatment. The lethal times (LT50 and LT90) of Nimbecidine (1 mg/L), chinaberry (6%) and the basil (8.4%) were 64 and 107 h, 41 and 73 h, 65 and 110 h, respectively. The females had a strong aversion to bean leaf surfaces sprayed with the sublethal concentrations of Nimbecidine (0.125-0.75 mg/L), chinaberry (0.75-3%) and basil (0.7-1.4%) oils. Significant decreases were recorded in the number of eggs laid on bean leaves sprayed with the sublethal concentrations for Nimbecidine (0.031-0.5 mg/L), chinaberry (0.75-3%) and the basil (1.4-5.6%) oils compared with unsprayed bean leaves. The study showed that the assessed concentrations of the oils obtained from the basil and chinaberry compared to the commercial botanical product (Nimbecidine) have similar biological effects on T. urticae.
A new Tetranychus Dufour (Acari: Tetranychidae) associated with Solanaceae from Turkey
(TÜBİTAK, 2015-01-01) Çobanoğlu, Sultan; Ueckermann, Edward Albert; Kumral, Nabi Alper; KUMRAL, NABİ ALPER; Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü.; 0000-0001-9442-483X; A-1388-2019
A new plant-feeding mite species, Tetranychus solanacearum Cobanoglu & Ueckermann sp. nov. (Acari: Tetranychidae), is described and illustrated. The samples were collected from various solanaceous plants (Solanaceae) [black nightshade (Solanum nigrum L.), bittersweet nightshade (Solanum dulcamara L.), eggplant (Solanum melongena L.), spiny nightshade (Solanum rostratum Dunal), jimsonweed or datura (Datura stramonium L.), and tomato (Lycopersicon esculentum L.)] in Ankara Province, Turkey. A key to all species of the genus Tetranychus Dufour known to occur in Turkey is presented in the paper.
Biolocigal features and life tables of Tetranychus urticae (Koch) (Acari: Tetranycidae) on different table tomato varieties
(Entomological Soc Turkey, Ege Univ, 2013-09-01) Atalay, Esra; Kumral, Nabi Alper; KUMRAL, NABİ ALPER; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü.; 0000-0001-9442-483X; A-1388-2019
The aim of this study was to determine the host plant resistance to T. urticae by comparing the biodemographic data of the mite, such as survival, development, oviposition, population fluctuation and the life table parameters of T. urticae on four table tomato varieties. Leaflets from four tomato varieties (Swanson, Super Red, Dante and Alsancak) were assayed with the spider mite to detect the mite's the egg hatching, development duration, survival, sex rates, intrinsic rate (rm), reproductive rate (Ro), mean generation time (To) and gross reproduction rate (GRR). The study showed that when T. urticae fed on different tomato varieties, there is no statistically difference among the total developmental time of the mite, although the differences among varieties were shown in terms of egg hatching and juvenile stages. Whereas, Ro (112,80 and 130,89), To (16,75 and 16,81) and GRR (115,7 and 131,02) of Alsancak and Dante varieties, respectively, were significantly higher than the mite feds on Super Red (Ro =60,4; To = 26,7; GRR = 66,9) and Swanson (Ro =49,4; To = 25,3; GRR = 56,9). The mite population level during 15 days on Alsancak varieties was significantly higher compared with that on other tomato varieties. Thus, the findings indicate a possible susceptible of the Alsancak, which is plant parasitic nematodes resistance variety, against T. urticae. Thus, the results indicate that Swanson variety is more resistant to T. urticae compared with the other tomato varieties.
Effect of washing method on the reduction of insecticide residues and quality characteristics of sweet cherry fruits
(Entomological Society Turkey, 2024) Hazarhun, Gülden; Kumral, Nabi Alper; Gümül, Burcu; Kolcu, Ayşenur; Ertaş, Simge; KUMRAL, NABİ ALPER; Ertaş, Simge; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Bölümü; 0000-0001-9442-483X; 0000-0002-4540-6915; A-1388-2019; KLA-8587-2024
Sweet cherry trees were sprayed with 5 insecticides (acetamiprid, dimethoate, lambda-cyhalothrin, malathion, tau-fluvalinate) at the recommended field doses in this study. Fruits were harvested after the pre-harvested interval for each pesticide completed and then they were immersed into tap water and three different washing solutions (with three different concentrations) for 3 minutes (at 20(o)C). Insecticide concentrations were detected with a multi-residual analysis method using LC-MS/MS in Bursa Uludag University in 2022. Following the treatments, changes in the quality characteristics of fruits were also investigated by quality (colour, texture and fruit cracking rate, water-soluble dry matter) and sensory analysis (fruit and stem colour, firmness, appearance, general acceptability). The results revealed that washing method with tap water during 3 min decreased insecticide residue level by 7-45% depending on insecticide active compound. Higher reduction rates were observed by washing with citric acid (10%), sodium bicarbonate (2.5%) and sodium hydroxide (0.5%). But significant reductions were detected only in lambda-cyhalothrin and malathion residues when compared with the newly harvested fruit samples. Processing factors (PF) of all washing methods were generally lower than 1 except for three treatments. PF values showed variations depending on the type of washing solution and the active compound of insecticides. Although washing with citric acid (10%), sodium bicarbonate (2.5%) and sodium hydroxide (0.5%) solutions caused reduction in residue levels, their negative effects on the quality and sensory characteristics of the fruits cannot be ruled out.
Farklı domates çeşitlerinde zararlılara karşı savunma yapıları olan trikom yoğunluğunun ve acylsugar konsantrasyonunun belirlenmesi
(Bursa Uludağ Üniversitesi, 2024-02-09) Gök, Narin; Özkan, Simge; Çobanoğlu, Sultan; KUMRAL, NABİ ALPER; AKBUDAK, NURAY; GENÇER, NİMET SEMA; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü; 0000-0001-9442-483X; 0000-0003-2669-5667; 0000-0001-8053-5002
Ülkemizde domates yetiştiriciliği ekonomik getiri ve beslenme yönünden önem arz etmektedir. Domates yetiştiriciliğindeki önemli sorunlardan biri zararlılar (böcek ve akar) nedeniyle yüksek ürün kayıplarıdır. Günümüzde bazı domates hastalıkları ve nematot zararlılarına karşı dayanıklı domates çeşitleri ruhsatlanmasına karşın; diğer zararlılara karşı dayanıklı tescilli çeşitler henüz kaydedilmemiştir. Domateslerde, böceklerin ve akarların zararına hatta biyolojik mücadele etmenlerinin aktivitesine karşı olumsuz olan unsurlardan biri de domatesin vejatatif organlarında bulunan keseli ve kesesiz trikomlardır. Kesesiz trikomlar bu canlıların vücudunu tahrip ederek olumsuz etki yaratırken; keseli trikomların içinde bulunan acylsugar’ın bunlar üzerinde zehir veya kaçırıcı etkisi bulunmaktadır. Bundan dolayı, bu çalışmada 49 domates çeşidinde bulunan trikom yapıları ve keseli trikomlardan salgılanan acylsugar konsantrasyonları araştırılmıştır. İncelenen domates çeşitlerindeki trikomların tiplerine göre yoğunluklarının saptanması zararlılara dayanıklı çeşitlerin belirlenmesi için temel bilgi sağlarken; aynı zamanda doğal düşmanların etkinliğini sınırlaması açısından da bilgi verilmiştir. Çalışmada yaprak, sap ve dallarda tip IV ve tip VI keseli; tip III ve tip V kesesiz trikomların yoğunlukları tespit edilmiştir. Her bir vejatatif organ ayrı ayrı değerlendirildiğinde farklı trikom tiplerinin yoğunluklarının çeşitlere bağlı önemli düzeyde değişiklik gösterdiği saptanmıştır. Ward’ın minumum varyans analizi metotuna göre her tipteki trikom yoğunlukları düşük, orta ve yüksek olarak kümelenmiştir. İncelenen çeşitler arasından bazılarının hem keseli hem de kesesiz trikom açısından düşük yoğunluğa sahip olduğu belirlenmiştir. Benzer istatistiki farklılıklar, keseli trikomlarda bulunan acylsugar içerikleri açısından da ortaya konmuştur.
The toxic effects of some acaricides on the tomato russet mite and its predator amblyseius swirskii athias-henriot, 1962 (Acari: Phytoseiidae)
(Ege Üniversitesi, 2023-01-01) Kolcu, Ayşenur; Kumral, Nabi Alper; Kolcu, Ayşenur; KUMRAL, NABİ ALPER; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bitki Koruma Anabilim Dalı; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü; 0000-0001-9442-483X; FII-8215-2022; A-1388-2019
The tomato russet mite, Aculops lycopersici(Massee, 1937) (Acari: Eriophyidae) is a common pest of tomatoes. The predatory mite, Amblyseius swirskiiAthias-Henriot, 1962 (Acari: Phytoseiidae), can control A. lycopersici populations. To integrate biological and chemical control of A. lycopersici, side effects of the lethal concentrations of acaricides, as a predator, on A. swirskii should be considered. The lethal concentrations of 14 acaricides for A. lycopersici were determined under laboratory conditions at Bursa Uludag University during 2017-2018. To understand the toxic impacts of the acaricides on juveniles and females of A. swirskii, the LC99 values for A. lycopersici of each acaricide were applied to A. swirskii. The reproduction reduction effects of the LC99 values were also assessed. Quite low concentrations of abamectin, milbemectin, pyridaben, azadirachtin and sulphur were found to be toxic for A. lycopersici. Based on the side effect scale, the LC99 values of abamectin, acequinocyl, bifenazate, fenproximate, fenbutatin oxide, hexythiazox, milbemectin and sulphur that killed A. lycopersiciwere found to be slightly toxic to both females and juveniles of A. swirskii. The results of this comparative toxicological study have showed that more field studies should be conducted to evaluate the effectiveness of using low concentrations of acaricides with A. swirskii in combination for controlling A. lycopersici.
Toxic effects of some acaricides on Aceria oleae (Nalepa, 1900) (Acari: Eriophyidae) and its predator Neoseiulus californicus (McGregor, 1954) (Acari: Phytoseiidae) under laboratory conditions
(Ege Üniversitesi, 2021-01-01) Kumral, Nabi Alper; Çobanoğlu, Sultan; Göksel, Pınar Hephızlı; Aksoy, Anıl; KUMRAL, NABİ ALPER; Göksel, Pınar Hephızlı; Aksoy, Anıl; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü.; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Entomoloji Anabilim Dalı.; 0000-0001-9442-483X; 0000-0002-1120-3925; 0000-0002-1025-2880; A-1388-2019; FBZ-1149-2022; CBB-0902-2022
Olive bud mite, Aceria oleae (Nalepa, 1900) (Acari: Eriophyidae) is one of the key pests that regularly needs control with acaricides in olive orchards of Bursa Province, Turkey. For the chemical control of A. oleae, it is critical the use of acaricides does not reduce the survival and fecundity of its natural enemies. The toxic effects of three concentrations of seven acaricides were assessed against both A. oleae and its predator Neoseiulus californicus (McGregor, 1954) (Acari: Phytoseiidae) using a residual method under controlled conditions at Bursa Uludag University during 2020-2021. The highest recommended concentrations of acequinocyl, azadirachtin, fenbutatin oxide, milbemectin, pyridaben, spirodiclofen and sulfur killed A. oleae adults with rates varying from 80 to 100%. Two concentrations of milbemectin, pyridaben and sulfur showed high mortality rates. Nevertheless, highest recommended concentrations of acequinocyl, pyridaben, spirodiclofen and sulfur were found to be highly toxic to N. californicus adults with rates varied from 82 to 100%. The high mortalities for mobile immature stages and reducing in the fecundity of N. californicus occurred by highest recommended concentrations of all tested acaricides. Based on the scale recommended by the International Organization for Biological Control, some sublethal concentrations of fenbutatin oxide, spirodiclofen and sulfur were found to be slightly harmful to both mature and immature of N. californicus.
Validation of LC-MS/MS method for simultaneous determination of chlorpyrifos, deltamethrin, imidacloprid and some of their metabolites in maize silage
(Taylor, 2022-01-13) Azar, İsmail; Kumral, Nabi Alper; Azar, İsmail; KUMRAL, NABİ ALPER; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bitki Koruma Bölümü; Bursa Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bitki Koruma Anabilim Dalı; 0000-0001-9442-483X; A-1388-2019; ENV-1088-2022
In this study, a validation of a multi-residue analysis method was performed for the simultaneous analysis of chlorpyrifos (CHL), deltamethrin (DEL) and Imidacloprid (IMI) residues and some of their metabolites in maize silage, by LC MS/MS. Extraction was conducted with acetonitrile acidified with 1% acetic acid. To avoid the matrix effect, a matrix matched calibration was used. The method was validated according to the SANTE/12682/2019 Guidelines. Selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), trueness (recovery %) and precision (intra-day and inter-day) parameters were evaluated in line with the SANTE document. The linearities of all compounds were quite confident (R-2 >= 0.98) and no interference was observed. The LOD and LOQ values were between 2.76 mu g kg(-1) to 53.61 mu g kg(-1) and 9.19 mu g kg(-1) to 178.71 mu g kg(-1), respectively. The recovery, repeatability RDSr and reproducibility RDSR values of compounds were calculated between 93.7-109.2%, 1-15%, and 1-13%, respectively. Consequently, results obtained with the evaluation of all parameters were found to be compatible with the SANTE validation criteria, so the method was reliable, effective and easy to use for the detection of insecticides and metabolites in maize silage with LC MS/MS.