Browsing by Author "Karaca, Mehmet Yılmaz"
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Item Determination of erythromycin, spiramycin, tilmicosin and tylosin in animal feedingstuffs by liquid chromatography-tandem mass spectrometry(Kafkas Üniversitesi, 2014-05) Özcan, Ali; Karaca, Mehmet Yılmaz; Yıbar, Artun; Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Hijyeni ve Teknolojisi Bölümü.; 38762296900Detection of macrolides residues in animal feedingstuffs to ensure food safety is very important issue. For this purpose, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of residues of 4 macrolides (erythromycin, spiramycin, tilmicosin and tylosin) in animal feedingstuffs. Roxithromycin (ROX), a macrolide not used in veterinary medicine, was used as internal standard. The mass spectral acquisition was done in the positive-ion mode applying multiple reaction monitoring with the following ions (mass-to-charge ratio, m/z): m/z 837.4 -> 679.2; m/z 734.4 -> 576.2; m/z 422.4 -> 174.1; m/z 869.1 -> 696.1 and m/z 916.4 -> 772.4 for roxithromycin (ROX), erythromycin (ERY), spiramycin (SPI), tilmicosin (TIL), and tylosin (TYL), respectively. Good repeatibility, reproducibility and recovery values was obtained. Average recoveries ranged from 98.9% (TIL) to 101% (ERY) and an overall mean of 99.9%. Method limits of detection (LODs) of ERY, SPI, TIL and TYL 5.1, 6.6, 6.7 and 7.5 mu g kg-1 were achieved respectively. Method limits of quantification (LOQs) were 8.5, 11.1, 11.2 and 12.4 mu g kg-1 for the same drugs, respectively. Satisfactory decision limits (CC alpha) and detection capabilities (CC beta) were also attained. The method is simple, rapid, sensitive and suitable for the simultaneous determination of macrolide antibiotics in animal feedingstuffs.Item Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry(Taylor & Francis, 2012) Okutan, Bülent; Özcan, Ali; Karaca, Mehmet Yılmaz; Çetinkaya, Figen; Yıbar, Artun; Soyutemiz, Gül Ece; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.; 0000-0002-3742-8831; 0000-0003-0128-5653; 0000-0001-9510-5734; AAI-1993-2021; FXX-0689-2022; H-8972-2018; 8657771200; 38762296900; 8416745200Analysis of residual levels of tetracyclines (TCs) in chicken meat was performed using a validated liquid chromatography coupled with a tandem mass spectrometry (LC-MS/MS) technique. Overall, the recoveries for TCs ranged from 56.9% to 101.2%, with standard deviations of 4.5-13.2%. Detection limits ranged from 7.9 to 14.6 mu g kg(-1). In four of 60 samples, doxycycline (DXC) was determined in a range from 19.9 to 35.6 mu g kg(-1); and in one sample tetracycline was detected at 17.2 mu g kg(-1). Chlortetracycline (CTC) and oxytetracycline (OTC) were not detected in any of the tested samples. This study indicates that chicken meat sold in Bursa, Turkey, contained some residues of TCs. Therefore, stricter regulations for the use of antibiotics in the poultry industry and the monitoring of drug residues in chicken meat prior to marketing are needed. Finally, this method has been applied successfully for the confirmation of TCs in chicken meat.