Browsing by Author "Kesici, Müge"
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Item Antioxidative enzyme activities in Brassica juncea L. and Brassica oleracea L. plants under chromium stress(WFL Publisher, 2011-01) Zaimoğlu, Zeynep; Başcı, Nihal; Budak, Fuat; Köksal, Nezihe; Kesici, Müge; Gülen, Hatice; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; ABD-6710-2020; 54380475800; 54412311700; 6603211102Chromium (Cr) is one of the most common heavy-metal contaminant in groundwater, soil and sediments. Cr is a toxic element that occurs in highly variable oxidation states. Since Cr (VI) is a strong oxidant, it may cause severe oxidative stress in plant tissue. However, the antioxidative responses of plants to excess Cr have not been studied critically. Thus, the present study was undertaken to assess antioxidative responses of Brassica juncea L. and Brassica oleracea L. plants as models because of their phytoremediation capacity of metal enriched soils under different levels of Cr (VI) treatments. Seedlings were grown in 15 cm x 17 cm pots containing turf mixture in a greenhouse for 6 weeks. Then plants were irrigated with the solutions containing 0, 0.05, 0.25, 0.5 and 1 mM Cr (VI) for 10 weeks. Fully expanded leaves were collected from the plants at the end of the treatments for catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) analyses. Even though both species showed a similar trend in response to Cr (VI) treatments, the total enzyme activities were higher in Brassica oleracea L. than in Brassica juncea L. Cr (VI) treatments significantly decreased the activities of CAT depending on the Cr concentrations in both species. After the significant increase, a sharp decrease was observed in the activity of APX and GR in both species. Data indicated that a coordinated increase in APX and GR activities in both Brassica species under Cr stress played a role as signals to protect the plants from Cr-induced stress.Item The assessment of the behavior of nanoparticles in environmental systems for sustainable development(Elsevier, 2012-09-23) Zaimoğlu, Zeynep; Budak, Dilek Bostan; Kesici, Müge; Gülen, Hatice; Ergin, Sergül; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAG-6558-2020Item Bazı çilek (Fragaria × ananassa) çeşitlerinin yüksek sıcaklığa toleransları(Uludağ Üniversitesi, 2009-01-20) Kesici, Müge; Gülen, Hatice; Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Bahçe Bitkileri Anabilim Dalı.Bazı çilek (Fragaria × ananassa) çeşitlerinin yüksek sıcaklığa toleranslarında genotipik farklılığın belirlenmesi amacıyla fizyolojik parametrelerden yararlanılan bu çalışmada, 11 çilek çeşidi [(Camarosa, CG3 (Cal-Giant), Elsanta, Fern, Festival, Honeoye, Kabarla, Redlands Hope, Selva, Sweet Charlie, Whitney)] kullanılmıştır. Denemeler iki temel prensipte yürütülmüştür. Kontrollü koşullarda uygulanan yüksek sıcaklığın bitkilerdeki fizyolojik etkilerini tespit etmek amacıyla frigo fideler, 5– 6 gerçek yapraklı oluncaya kadar serada ortalama 15/30ºC (gece/gündüz) sıcaklıkta, ~%65 nemde yetiştirilmiştir. Bitkiler yüksek sıcaklık stresi denemeleri için laboratuardaki iklimlendirme kabinine alınarak, kabin sıcaklığı kademeli olarak 35, 40, 45 ve 50ºC’ye yükseltilmiş ve her bir sıcaklık derecesinde 24 saat tutulmuştur. Her bir sıcaklık kademesindeki bitkilerden alınan yaprak örneklerinde, yaprak oransal su kapsamı (YOSK), turgor kaybı (TK), klorofil miktarı (KM) ve lipid peroksidasyonu (MDA) ölçümleri yapılmıştır. YOSK sonuçlarına göre, Elsanta ve R.Hope en yüksek YOSK’na sahip iken Festival ve CG3 en düşük değere sahip olmuştur. Buna karşılık TK’nda, Elsanta ve R.Hope en düşük TK’na sahip iken Festival ve CG3 en yüksek TK’na sahip çeşitler olmuştur. KM’nda ise Elsanta ve R.Hope en düşük değişim oranını gösterirken buna karşılık CG3 ve Whitney çeşitleri en yüksek değişimi göstermişlerdir. Bunun yanında, MDA sonuçlarına göre, en düşük MDA miktarına Elsanta ve Fern çeşitleri sahip iken, Festival ve Honeoye çeşitleri en yüksek MDA miktarına sahip olmuşlardır. Çilek çeşitlerinin yüksek sıcaklık toleranslarını belirlemek için, serada yetiştirilen bitkilerden alınan yaprak örnekleri, kontrollü olarak yüksek sıcaklık uygulamasının yapıldığı sıcak su banyosunda 60ºC’ye kadar kademeli olarak tutulmuş ve iyon sızıntısı testi sonucuna göre her bir çeşide ait yüksek sıcaklık tolerans dereceleri belirlenmiştir. Buna göre sıcaklığın artmasıyla birlikte hücresel zararlanmaya bağlı olarak çeşitlerin yüksek sıcaklığa toleranslarında düşüş olduğu tespit edilmiştir. Elde edilen sonuçlara göre çeşitlerin sıcaklık stres tolerans noktaları 51,8 ve 52,9ºC arasında değiştiği belirlenmiştir. Sonuç olarak, değerlendirilen 11 çilek çeşidi arasında Elsanta, Redlands Hope ve Camarosa çeşitlerinin yüksek sıcaklığa göreceli olarak tolerant oldukları, Whitney, Fern, Festival ve CG3 çeşitlerinin ise nispeten daha hassas çeşitler olduğu ortaya konulmuştur. Çalışmadan elde edilen tüm sonuçlar değerlendirildiğinde yüksek sıcaklığa toleransta çilek çeşitleri arasındaki genotipsel farklılıklar ortaya konularak bundan sonra çilekte yüksek sıcaklığa tolerans mekanizmasına yönelik yapılacak moleküler biyolojik çalışmalar için zemin oluşturmuştur.Item Changes in antioxidant enzyme activities during cold-acclimation in sweet cherry cultivars grafted on different rootstocks(WFL Publication, 2013) Cansev, Asuman; Kesici, Müge; Uludağ Üniversitesi/Ziraat Fakültesi/Tarla Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-4255-2019; ABD-6710-2020; 26326677200; 54412311700Plant acclimation to environmental stress is governed by several mechanisms including alterations in antioxidant defense systems. The purpose of this study was to investigate the effects of Gisela 5 and Mazzard rootstocks on the cold-hardiness of the sweet cherry cultivars (cvs.) 0900 Ziraat and Lambert, as well as to analyze the changes in activities of antioxidative enzymes including catalase (CAT; EC 1.11.1.6), soluble peroxidase (S-PRX; EC 1.11.1.7), cell wall-bound peroxidase (CWB-PRX; EC 1.11.1.7) and glutathione reductase (GR; EC 1.6.4.2) in cold-acclimated (CA) and non-acclimated (NA) stages. One-year shoots of 4 years-old sweet cherry tree cvs. 0900 Ziraat and Lambert grafted either on Gisela 5 or Mazzard rootstock were collected in CA (in January) or NA (in July) stage and subjected to artificial low temperature tests (4 degrees C, -5 degrees C, -15 degrees C and -25 degrees C) in order to determine the degree of cell membrane injury in bark tissues by ion leakage method. Antioxidant enzyme activities were assayed in another set of samples that were not subjected to low temperature treatments. Our data showed that ion leakage in bark tissues was greater in NA stage compared to CA stage. Ion leakage was below 50% in barks exposed to 4 degrees C and -5 degrees C in either period and above 50% in those exposed to -15 degrees C and -25 degrees C in NA stage. Ion leakage was lower if cultivars were grafted on Mazzard compared with grafting on Gisela 5. In addition, activities in both cultivars of CAT, as well as S-PRX and S-PRX, but not GR, were significantly higher during CA stage compared with those in NA stage. Elevations in CAT and PRX (both cell wall and soluble) activities were more prominent if the cultivars were grafted on Mazzard rootstock. We conclude that, cold hardiness in the sweet cherry involves changes in certain antioxidant enzyme activities depending usually on whether the plant is in CA or NA stage. Our finding that activities of CAT, as well as S-PRX and CWB-PRX in cultivars grafted on Mazzard were higher than those in cultivars grafted on Gisela 5 suggests that cold hardiness may be affected by the rootstock.Item Effects of high temperature stress on enzymatic and nonenzymatic antioxidants and proteins in strawberry plants(TÜBİTAK, 2016-12-03) Ergin, Sergül; Gülen, Hatice; Kesici, Müge; Turhan, Ece; Köksal, Nezihe; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 6603912487The mechanism of tolerance to high temperatures was investigated in two strawberry (Fragaria x ananassa Duch) cultivars, 'Redlands Hope' ('R. Hope', heat tolerant) and 'Cal. Giant 3' ('CG3', heat sensitive). Leaves were collected from plants that were exposed to gradual heat stress and heat-shock stress separately. The contents of nonenzymatic antioxidants such as ascorbic acid (AsA) and glutathione (GSH) and the activities of enzymatic antioxidants such as ascorbate peroxidase (APX) (EC 1.11.1.11), catalase (CAT) (EC 1.11.1.6), and glutathione reductase (GR) (EC. 1.6.4.2) were measured followed by heat treatments. Additionally, proline content was determined, and heat shock proteins (HSPs) were analyzed with an immunoblotting method to investigate protein markers involved in the heat-stress tolerance of strawberry plants. The contents of AsA and GSH did not change depending on heat stress type, temperatures, or cultivars. While APX and CAT activities increased with high temperatures, GR activity was almost unchanged. The proline content of the cultivars increased in both treatments. Anti-HSP60 immunoblots revealed that a 23 kDa polypeptide was detected during the heat acclimation of strawberry cultivars. The intensity of the heat shock protein in 'R. Hope' plants was more than in 'CG3' plants. Thus, the accumulation of 23 kDa heat shock protein was correlated with the heat tolerance of the cultivars. In conclusion, strawberry leaf tissues of 'R. Hope' were found to enhance the structural stability of cellular membranes under high temperature by increasing both the activity of such enzymes as CAT and APX to activate the antioxidative systems and the expression of 23 kDa HSP.Item Effects of high temperature stress on total phenolic content and antioxidant capacity in strawberry plants(Elsevier, 2012-09-23) Cansev, Asuman; Kesici, Müge; Ergin, Sergül; Gülen, Hatice; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; ABD-6710-2020; AAH-4255-2019; AAG-6558-2020Item Genotype-dependent gene expression in strawberry (Fragaria x ananassa) plants under high temperature stress(Springer/Plenum Publishers, 2020-06-04) Ergin, Sergül; Gülen, Hatice; İpek, Ahmet; Ersoy, Figen; Kesici, Müge; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Moleküler Biyoloji ve Genetik Bölümü.; 0000-0002-9136-3186; 0000-0003-2267-069X; AAI-6817-2021; ABD-6710-2020; 54412311700; 6603912487; 55088196700The differences in tolerance to high temperatures were investigated on the basis of gene expressions in two strawberry (Fragaria x ananassaDuch) cultivars which were previously determined as high temperature tolerant (Redlands Hope = R. Hope) and sensitive (Festival). Plants were exposed incrementally to 35, 40, 45, and finally 50 degrees C for 24 h. qRT-PCR analyses were carried out with 19 known sequences from the databases. Protein expression analyses were based on SDS-PAGE results, sequenced and then separated due to their isoelectric points. Expression levels were determined at 35, 40, and 45 degrees C. According to the results, tolerance of 'R. Hope' to high temperature stress can be explained with the coordination of Hsp70, Hsp90, and small heat shock proteins (sHsps) having a vital and supplementary role in stress response. Sensitive cultivar 'Festival' can respond to high temperatures only with the low molecular weight protein and transcripts that do not take a central role in high temperature stress response. Moreover, allergen gene expression triggered by high temperature were detected in both cultivars with different expression levels. The greater expression level in allergen genes observed in the sensitive cultivar 'Festival' under high temperature indicates that there is possibly a negative correlation between expression level in allergen genes and heat stress tolerance. Future studies addressing allergen gene expression under high temperature stress are required to confirm on these findings and to expand on the potential use as a molecular marker in breeding process for enhanced tolerance to high temperature.Item Heat-stress tolerance of some strawberry (fragaria × ananassa) cultivars(University Agricultural Science & Veterinary Med Cluj-Napoca, 2013) Ergin, Sergül; Turhan, Ece; Köksal, Nezihe; Kesici, Müge; Gülen, Hatice; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0001-7586-3108; ABD-6710-2020; AFW-5375-2022; 54412311700; 6603211102; 6603912487Physiological parameters were used to investigate genotypic variations in 15 strawberry cultivars ['Aromas', 'Camarosa', 'Carmine','Cal. Giant 3' (CG3), 'Cal. Giant 5' (CG5), 'Elsanta', 'Fern', 'Festival', 'Honeoye', 'Kabarla', 'Redlands Hope' (R.Hope), 'Ruby Gem', 'Selva', 'Sweet Charlie' and 'Whitney'] and their relationship to heat-stress tolerance (HST). Cold stored (frigo) strawberry seedlings were grown in pots for six weeks and then transferred to a growth chamber. The temperature in the growth chamber was increased stepwise from 35 to 40,45 and 50 degrees C to create a heat-stressed environment. Leaf relative water content (RWC), loss of turgidity and chlorophyll content were measured at each temperature. The 'Elsanta' and 'R.Hope' had the highest RWC, while the 'Festival' and 'CG3' had the lowest. However, 'Elsanta' and 'R.Hope' had the lowest loss of turgidity, while 'Festival' and 'CG3' had the highest. 'Elsanta' and 'R.Hope' showed the lowest chlorophyll content, and 'CG3' and 'Whitney' had the highest. To determine HST (LT50), leaf discs of each cultivar were exposed to 35, 40, 45, 50, 55 and 60 degrees C. A considerable decrease in the LT50 was observed with increasing temperature in all cultivars. The LT50 of the cultivars ranged from 51.8 to 52.9 degrees C. Based on the data collected, 'Elsanta', 'R. Hope' and 'Camarosa' were determined to be relatively heat-tolerant cultivars, while 'Whitney', 'Fern', 'Festival' and 'CG3' were heat-sensitive cultivars.Item Peroxidase activity and lipid peroxidation in strawberry (fragaria x ananassa) plants under low temperature(Uludağ Üniversitesi, 2008) Gülen, Hatice; Çetinkaya, Cem; Kadıoğlu, Murat; Kesici, Müge; Cansev, Asuman; Eriş, Atilla; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.The activity of peroxidase (PRX) isozyme, lipid peroxidation (Malondialdehyde, MDA content) and cell membrane injury were studied during low temperature treatment for different periods in strawberry (Fragaria x ananassa cv. Camarosa) leaf tissues. Seedlings were grown for six weeks (plants had 4-5 leaves) in a greenhouse then the plants were transferred to a climate chamber with constant 5o C, 60% relative humidity, 14/10 h (light/dark) photoperiod regime and 4 LS light intensity for 1, 4, 7 or 10 days to impose a low temperature stress. In general, low temperature application during 10 days caused a linear increase in MDA content. Native polyacrylamide gel electrophoresis (PAGE) of both acidic and basic peroxidase (PRX) isozymes yielded a single sharp protein band with Rf=0.23 and Rf=0.17, respectively. In addition data indicated a strong relationship between band intensities and the duration of the low temperature treatment. However, the considerable increase of PRX activities could not stop the deleterious effects of low temperature, but reduced severity of stress, thus showing a reduction in the percentage of injury on the 7th day which is correlated with cold-acclimation of strawberry leaf tissues under low temperature.Item Proline and antioxidant enzyme activities in some strawberry cultivars under drought and recovery(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2018-02-19) Gülen, Hatice; Kesici, Müge; Ergin, Sergül; Çetinkaya, Cem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 57202266058This study examines the molecular effects of drought and recovery applications on four strawberry (Fragaria x ananassa Duch) cultivars known as relatively heat-tolerant ['Redlands Hope' ('R. Hope') and 'Camarosa'] and heat-sensitive ['Festival' and 'Cal. Giant-3' ('CG-3')]. After frigo seedlings were grown in a greenhouse for eight weeks (until they had five-six leaves) in a pot, 10% Poliethyleneglycol 6000 (PEG) was applied in order to compose drought condition for 15 days. Following PEG application, plants were watered without PEG for 15 days to observe the recovery. Leaf relative water content (RWC), loss of turgidity, cell membrane injury, proline content and the activities of ascorbate peroxidase (APX, EC 1.1.11.1), glutathione reductase (GR, EC 1.6.4.2) and peroxidase (PRX, EC 1.11.1.7) were determined in leaf tissues taken from plants at the end of the drought and recovery applications. Although membrane injury was increased by the PEG application, it reduced almost to the control levels at the end of the recovery period. Proline contents were found higher in cvs. 'R. Hope' and 'Camarosa' than cvs. 'Festival' and 'CG-3' under drought conditions. APX and GR enzymes showed great activities in drought applied plants of heat-tolerant cultivars. Native polyacrylamide gel electrophoresis (PAGE) of basic PRX isozymes yielded three protein bands with Rf = 0.45, Rf = 0.55 and Rf = 0.60 that were sharply increased their activities by PEG and recovery applications in cvs. 'R. Hope' and 'Camarosa'. In addition, leaves of all cultivars exposed to drought rolled up and had brown colour in various level as an indication of tolerance. Indeed 'Camarosa' and 'R. Hope' showed better recovery performance after rewatering than 'Festival' and 'CG-3'. All data indicated that heat-tolerant cultivars showed relatively drought tolerance in this study.Item Protein and peroxidase activity in strawberry plants under the combined effects of drought, low and high temperature(Elsevier, 2012-09-23) Kesici, Müge; Cansev, Asuman; Ergin, Sergül; Gülen, Hatice; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; ABD-6710-2020; AAH-4255-2019; AAG-6558-2020Item Proteins in strawberry plants (Fragaria x ananassa Duch.) under heat stress(Elsevier, 2012-09-23) Turhan, Ece; Köksal, Nezihe; Ergin, Sergül; Gülen, Hatice; İpek, Ahmet; Kesici, Müge; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAG-6558-2020; ABD-6710-2020