Browsing by Author "Nak, D."
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Publication Ascorbic acid inclusion in semen extender improves the post-thawed semen quality of sahiwal cattle (Bos indicus)(Zoological Society of Pakistan, 2015-12-01) Sohail, M. U.; Shahzad, A. H.; Iqbal, S.; Shabbir, M. Z.; Iqbal, Z.; Abbas, S.; Younus, M.; Nak, D.; Nak, Y.; Arshaad, T.; NAK, DENİZ; NAK, YAVUZ; FPJ-3706-2022; AAH-5494-2021The antioxidant effects of ascorbic acid at different inclusion rates were evaluated upon semen (n=150) from mature Sahiwal cattle bulls (Bos indicus, n = 6) in summer season. The semen was diluted in Tris-citrate egg yolk extender containing different levels (0.0, 1.0, 2.0, 3.0, and 4.0 mg/mL) of ascorbic acid at 37.0 degrees C. Semen was cryopreserved at -196 degrees C in 0.5 mL French straws. Semen straws were thawed at 37 degrees C to assess the spermatozoa indices in terms of motility, viability, plasma membrane and acrosomal integrity under phase-contrast microscope. Supravital staining, hypo-osmotic swelling test and normal acrosomal reaction analysis tests were also performed for viability, plasma membrane and acrosomal integrity, respectively. The data were subjected to one way ANOVA. The results revealed significant improvement (P< 0.05) in post thaw sperm quality in terms of motility, vitality, acrosomal and plasma membrane integrity by the increasing concentrations of ascorbic acid (1.0, 2.0, 3.0, 4.0 mg/mL) in semen extender. Quality parameters were higher (P< 0.05) when 3.0 mg/mL of ascorbic acid was added in semen extender followed by 2.0 and 4.0 mg/mL. In conclusion the addition of ascorbic acid at the rate of 3.0 mg/mL in the semen extender may improve the semen quality of Sahiwal cattle bull.Publication Sry-negative xx sex reversal in an english cocker spaniel: A case report(Czech Academy Agricultural Sciences, 2015-01-01) Shahzad, A. H.; Nak, D.; NAK, DENİZ; Gülten, T.; Karkucak, M.; Yılmaz, R.; Nak, Y.; NAK, YAVUZ; Şimşek, G.; Bursa Uludağ Üniversitesi/Veterinerlik Fakültesi; 0000-0002-0944-2230; 0000-0002-8623-8292; ABG-8940-2020; AAH-5494-2021A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosome which supports the hypothesis that some other genes are also responsible for testicular development. The present case report describes a one-year-old dog brought to the hospital with a complaint of no oestrus cycle. Apparently normal masculine bitch had an enlarged clitoris with an os on radiograph. A gonado-hysterectomy was performed. Instead of ovarian structures both testicular masses were present and a normal complete bicornuate uterus with a normal cervix was observed. The histology of both gonads showed two testes, each with its ductus deferens and the uterus with typical histological structure. Cytogenetic evaluation of peripheral blood lymphocytes revealed a female with a normal karyotype (78, XX). Polymerase chain reaction (PCR) analysis of genomic DNA showed that the SRY gene was absent.