Browsing by Author "Njoku, Kelechi Longinus"
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Item Phytoremediation of crude oil contaminated soil using glycine max (Merril); through phytoaccumulation or rhizosphere effect?(Uludağ Üniversitesi, 2016-12-01) Njoku, Kelechi Longinus; Akinola, Modupe Olatunde; Oboh, Bolanle OlufumilayoThe aim of this study was to evaluate the process which Glycine max (soybean) uses in the phytoremediation of crude oil contaminated soil. A screen house experiment was conducted with different amounts (25g, 50g and 75g) of crude oil-contaminated soil for 110 days. The initial and final total petroleum hydrocarbon (TPH) contents of the contaminated soils and that in the plant tissues were measured and the bacterial loads and types in the soil samples were determined at the end of the study. The soil pH, moisture and organic matter contents were also determined every 21 days for 110 days. Soil samples for the above analyses were obtained from the soils treated with the various amounts of crude oil with and without G. max (which served as the control). The investigation revealed that the initial TPH values of the soils were higher than the final TPH values and that there were lower TPH values in the soils with G. max compared to soils without G. max. The growth of G. max led to 52.48% reduction against 50.15% reduction in non-vegetated soil, 66.93% reduction against 44.57% reduction in non-vegetated soil and 49.04% reduction against 44.31% reduction in soil contaminated with 25g, 50g and 75g crude oil respectively The bacterial load, pH, moisture content and the organic matter contents of the crude oil contaminated soil were significantly affected by the growth of G. max at different levels of significance (P<0.05; P<0.01; P<0.001). The results of this study have shown that the growth of G. max on crude oil contaminated soil reduces the TPH level, enhances bacterial growth, improves the soil pH and improves the moisture content (for high level contamination). Thus, it is suggested that G. max is a good candidate for remediating crude oil contaminated soil and that it remediates crude oil contaminated soils through rhizospheric effect.Item Vermiremediation of crude oil contaminated soil using eudrillus euginae and lumbricus terrestris(Uludağ Üniversitesi, 2017-04-22) Njoku, Kelechi Longinus; Nomba, Emmanuella Uche; Olatunde, Akinola ModupeThe negative and cost implications of remediation of petroleum contaminated sites using physical and chemical techniques have necessitated the use of biological techniques like vermiremediation. In this study the individual and synergistic abilities of two earthworms – Eudrillus euginae and Lumbricus terrestris to clean up crude oil contaminated soil were evaluated. It involved experimentally contaminating the soils with various quantities of crude oil. The total petroleum hydrocarbon (TPH), pH and nutrient contents of the soils were determined immediately after contamination and after thirty days of earthworm activities. Data obtained were statistically analysed using graphpad prisms 6.0 and SPSS 20.0 softwares. Activities of E. euginae led to 88.50% TPH loss, L. terrestris led to 76.42% loss while combined activities of the two earthworms led to 73.06% loss of TPH from the soil contaminated with 3ml crude oil after 30days. In soil without any earthworm but contaminated with same amount of crude oil there was only 21.19% loss of TPH for same period of time. Similar trends were observed in soils contaminated with 1ml and 2ml of crude oil for same period of study. The pH, sulphate, phosphate, nitrate and organic matter contents of the contaminated soils were reduced after 30 days and the reduction was more in soils with the earthworms than in the soils without the earthworms. The findings of this study show that the E. euginae and L. terrestris can help in the remediation crude oil contamination soil and that they are more effective individually than in a combined form. Also E. euginae had more impact on the contaminated soil than L. terrestris.