Browsing by Author "Onus, A. N."
Now showing 1 - 4 of 4
- Results Per Page
- Sort Options
Item Effects of antioxidant enzymes on heat stress tolerance of pepper (Capsicum annuum L.) seedlings(International Society for Horticultural Science, 2016) Turhan, Ece; Onus, A. N.; Gülen, Hatice; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0001-7586-3108; AFW-5375-2022; AAK-4655-2021; 6603211102; 6603912487Pepper seedlings (Capsicum annuum L. 'Amazon' and 'Kekova') were grown for 4 weeks at 25/10 degrees C day/night temperatures in a greenhouse and watered on a needs basis avoiding any additional stress factors. Gradual and shock heat stress (GHS and SHS) were applied (from 35 up to 50 degrees C) to the plant in a growth chamber and then heat stress tolerance (HST) was estimated. During the heat treatments, the activities of ROS producing enzyme, NADPH oxidase and ROS scavenging enzymes, catalase (CAT) and ascorbate peroxidase (APX) were measured besides leaf relative water content (RWC) and loss of turgidity. Leaf RWC decreased gradually from control to the highest temperature, while loss of turgidity increased in both heat stress types. Under GHS, HST (LT50) was calculated as 47.1 degrees C for Amazon and 45.6 degrees C for 'Kekova'. On the other hand with SHS, HST for 'Amazon' and 'Kekova' were determined as 47.4 and 42.8 degrees C, respectively. The results clearly show that the cultivar 'Amazon' is superior with respect to its antioxidant defence systems and should be more tolerant than 'Kekova' due to higher ROS-scavenging systems.Item Effects of morphogenetic and diurnal variability on the hypericin content of St. John's Wort (Hypericum Perforatum L.) populations originating from Turkey(Int Soc Horticultural Science, 2009) Turgut, K.; Onus, A. N.; Mathe, A.; Kaçar, Oya; Göksu, Erdinç; Azkan, Nedime; Uludağ Üniversitesi/Ziraat Fakültesi/Tarla Bitkileri Bölümü.; AAG-7449-2021; 24466299800; 24466217100; 24466251500This research was conducted to investigate effects of morphogenetic and diurnal variability on the hypericin content of St. John's Wort (Hypericum perforatum L.) populations originating from Turkey for maximum utilization of the active substance in plants. During 2002 and 2003, field trials were conducted at Uludag University, Faculty of Agriculture, Department of Field Crops, Bursa. Samples of bud, flower and capsule of H. perforatum L. populations were collected in the second year of cultivation (2003). Factorial arrangements of three H. perforatum L. populations (Bursa, Edirne, Izmir), three part of plant (bud, flower, capsule) and six collection hour (8.00, 10.00, 12.00, 14.00, 16.00, 18.00) were evaluated in a completely randomized design with three replications. Hypericin content (%) in parts of H. perforatum L. populations was determined according to DAC (1986). Consequently, the content of hypericin in the examined populations varied from 0.260% in Bursa to 0.283% in Izmir. Evaluating parts of plant, we also found hypericin content both in floral parts (0.309%) and buds (0.308%) were higher in comparison to capsule tissues (0.208%) for all populations. When collection hours were examined for the hypericin content of plants, the highest content was recorded at 10.00 (0.279%) and the lowest value at 16.00 (0.272%) and 18.00 (0.272%). As a result of this study, it is possible to say that the highest hypericin ratio was determined in flowers and buds generally collected at 8.00 between 10.00 pm with in a day for examined populations.Item Effects of soil water deficit at different growth stages on yield and quality of processing tomato(International Society for Horticultural Science, 2016-11-08) Onus, A. N.; Turhan, Ahmet; Kuşcu, Hayrettin; Özmen, Neşe; Aydınol, Pınar; Seniz, Vedat; Demir, Ali Osman; Uludağ Üniversitesi/Mustafakemalpaşa Meslek Yüksekokulu/Bitkisel Üretim Bölümü.; Uludağ Üniversitesi/Mustafakemalpaşa Meslek Yüksekokulu/Gıda Teknolojisi Bölümü.; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; Uludağ Üniversitesi/Ziraat Fakültesi/Biyosistem Mühendisliği Bölümü.; 0000-0002-1976-8082; 0000-0001-9600-7685; AAH-4682-2021; AAG-5889-2021; AAG-5956-2021; 57196504252; 23667971600; 25623754300; 48361146600; 13604787100; 7102184446In order to assess the effect of soil water deficit (SWD) at different growth stages, on yield and quality of processing tomato under deficit irrigation in the sub humid climate, an open-field experiment was carried out in 2010 and 2011 in the experimental site of the Mustafakemalpasa Vocational School, Uludag University, Turkey. A randomized complete block design with three replications was used. Four known growth stages the plant (V: vegetative, F: flowering, Y: yield formation and R: ripening) were considered and a total 15 irrigation treatments were applied (VFYR, FYR, VFY, VFR, VYR, VF, VY, VR, FY, FR, YR, V, F, Y and R). The effect of SWD at any stage of growth on fruit yield suitable for processing, soluble solid content, dry matter content, pH, total sugar, total acidity, lycopene, carotene and vitamin C, were evaluated. The results revealed that fruit yield and all quality parameters considered in this study were significantly affected by SWD due to omitted irrigation during the sensitive flowering and yield formation. Water deficits occurring during ripening stage increased values of soluble solid content, dry matter content, total sugar, total acidity, lycopene and total carotene. Highest yields were observed in the fully irrigated control (VFYR) and the treatment which allowed SWD during the vegetative stage (FYR). Even irrigations omission during one of the sensitive growth stages, caused up to a 10% loss of fruit yield. Much greater losses of 52-64% could be expected as a result of prolonged SWD during flowering and yield formation stages. In particular, relatively to the sub-humid areas of tomato cultivation, the best compromise between quality and quantity of the processing tomato fruit was achieved with omitted irrigations during only ripening stage (VFY treatment) although yield loss was about 5%.Item Screening garlic EST sequences for SSR motifs(International Society for Horticultural Science, 2016) Onus, A. N.; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; AAK-4655-2021; 16031208900; 6603912487SSR markers are one of the most preferable DNA molecular markers in plant genetic analyses because they are multi allelic, polymorphic, reproducible and transferable among the genetically close species although their development is costly and time consuming. Development of SSR markers can be accomplished with either sequencing of genomic library colonies containing SSR repeats or mining available sequences of related species in gene banks. The second method has been preferred in recent studies due to the dramatic increase in available nucleotide sequences of plant species. For garlic genome, limited SSR markers have been developed so far and development of more SSR markers is needed for detailed genetic characterization and mapping of garlic genotypes. In this study, we screened available garlic EST sequences for SSR motifs in order to generate EST-based SSR markers. We searched repeat motifs containing 2-6 nucleotides. The minimum repeat units criteria was ten for dinucleotides, seven for trinucleotides, five for tetranucleotides, and four for pentanucleotides and hexanucleotides. In total, 86 SSR motifs have been determined using these criteria and the most abounded repeat motifs were determined as (AT/TA)n. Primer pairs designed for some of these SSR motifs were observed as multi allelic and polymorphic among the garlic clones.