Browsing by Author "Oran, Seyhan"

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Additions to the lichen biota of the Kaz mountains (Çanakkale-Balıkesir, Turkey)
(Blam E V, 2020-12-01) Oran, Seyhan; ORAN, SEYHAN; Bursa Uludağ Üniversitesi; 0000-0001-8196-2065; AAH-5039-2021
In the present paper, 195 lichen taxa and one lichenicolous fungus are reported from the Kaz Mountains. Rhizocarpon jemtlandicum and Rinodina moziana are new to Turkey.
Analysis of the distribution of epiphytic lichens in the oriental beech (Fagus Orientalis lipsky) forests along an altitudinal gradient in Uludag Mountain, Bursa - Turkey
(Pakistan Botanical, 2010-08) Öztürk, Şule; Oran, Seyhan; Güvenç, Şaban; Dalkıran, Nurhayat; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-8196-2065; 0000-0001-8724-9981; AAG-7065-2021; AAH-5039-2021; AAD-7322-2019; ABE-6749-2020; 7004555373; 14067648200; 6602344044; 6508318183
A study of the diversity and distribution of epiphytic lichens were compared along an altitudinal gradient (900-1400 m) in the Fagetum zone of Uludag Mountain. Twenty four species of epiphytic lichens were analyzed. The most frequent species were Lecanora argentata, Lecanora carpinea, Lecanora chlarotera, Lecanora intumescens, Lecidella elaeochroma, Melanelixia subaurifera, Parmelia sulcata and Scoliciosporum umbrinum. The distribution of epiphytic lichens was significantly related to altitude. The species composition of epiphytic lichens at high altitudes was distinctly different than that of at low altitudes. The effect on the distribution of epiphytic lichens of altitude was statistically significant.
Antioxidant and antimicrobial potential, and HPLC analysis of stictic and usnic acids of three Usnea species from Uludag mountain (Bursa, Turkey)
(Shaheed Beheshti, 2015-01) Oran, Seyhan; Şahin, Saliha; Şahintürk, Pınar; Öztürk, Şule; Demir, Cevdet; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı/Moleküler Farmakoloji Laboratuvarı.; 0000-0001-8196-2065; 0000-0003-1508-0181; 0000-0003-1508-0181; 0000-0002-9381-0410; AAH-5039-2021; AAH-2892-2021; AFR-1890-2022; AAD-7322-2019; ABA-2005-2020; 14067648200; 15027401600; 55342852700; 7004555373; 7003565902
In this study, antioxidant and antimicrobial potentials of Usnea intermedia, U. filipendula, and U. fulvoreagens and their stictic and usnic acid contents were investigated. Antioxidant activity and total phenolic contents were evaluated in acetone, ethanol, and methanol extracts of these three species. Antioxidant activity was measured by ABTS [2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid)] method and total phenolic contents were measured by Folin-Ciocalteu method. High-performance liquid chromatography (HPLC) was used for the determination of lichen acids. It can be concluded from stictic and usnic acids contents that the order of solvent efficiency is acetone > ethanol > methanol and acetone > methanol > ethanol, respectively. Broth microdilution method was performed to determine the minimum inhibitory concentration (MIC) of the methanol extracts of three Usnea species. The MIC values of all the extracts ranged from 64 mu g/mL to 512 mu g/mL for all the bacterial strains that were tested in this study, and all the Fluoro quinolone-resistant Escherichia coli isolates (except for E101) were sensitive to the methanol extracts of the three Usnea species. This paper is the first study to determine the stictic acid content in U. intermedia and U. filipendula. Our findings indicate that these three Usnea species could be used as antioxidant and antimicrobial agents.
Changes of photosynthetic pigment content in lichens collected from urban and rural localities in Bursa province
(Bursa Uludağ Üniversitesi, 2019-06-21) Akyiğit, Feyza; Demir, Mesut; Öztürk, Şule; Oran, Seyhan; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.
In this study, contents of photosynthetic pigment in foliose Melanelixia subaurifera, Parmelia sulcata and fruticose Evernia prunastri, Ramalina farinacea were compared. Lichen species were collected from five localities in Bursa. Chlorophyll a, chlorophyll b, total chlorophlly, total carotenoid contents (mg/g), chlorophyll a/b ratio, total carotenoid/total chlorophyll ratio and phaeophytinization (OD435/OD415) ratio in the lichen extracts were differently determined between localities. These changes were found statistically significant (p≤0.05). The chlorophyll degradation rate at the localities in urban environments is determined to be higher than in the natural environment. It was observed that the air quality exposure ratio was related to the lichen morphology and thus the fruticose lichen species were more sensitive than foliose.
Comparison of photosynthetic pigment contents in lichen samples were collected from different localities in Bursa
(Uludağ Üniversitesi, 2017-09-10) Karakaş, Vesile Ebru; Öztürk, Şule; Oran, Seyhan; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
In this study, contents of photosynthetic pigment in foliose Hypogymnia physodes (L.) Nyl. and fruticose Pseudevernia furfuracea (L.) Zopf were compared. Lichen species were collected from five localities at Osmangazi and İznik districts in Bursa. Chlorophyll-a, chlorophyll-b, total carotenoid contents (mg/g), chlorophyll-a/chlorophyll-b ratio, total carotenoid/total chlorophyll ratio and OD435/OD415 ratio in the lichen extracts were differently determined between localities. These changes were found statistically significant (p<0.001). The highest chlorophyll-a contents were measured in Osmangazi-Soğukpınar (2.79±0.17 m/g), İznik-İhsaniye (2.37±0.24 mg/g), the lowest contents of chlorophyll-a were measured in İznik-Sağırhisar (2.02±0.21 mg/g), İznik-Nüzhetiye (2.22±0.19 mg/g). These results have been observed that Soğukpınar and İhsaniye localities were less affected by anthropogenic effects due to their presence in the rural areas. Whereas, the localities of Sağırhisar and Nüzhetiye were affected by agricultural activities. Only, the difference between total carotenoids/total chlorophyll ratio was not statistically significant. In addition, it has been found between the lichen species. The changes in the content of photosynthetic pigments of Pseudevernia furfuracea were much more than Hypogymnia physodes. These results showed that negative atmospheric conditions are more effective at fruticose lichens than at foliose lichens.
Contributions to lichenized fungal diversity of Gorukle Vampus area (Bursa Uludag University-Bursa, Turkey)
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2019-01-01) Oran, Seyhan; ORAN, SEYHAN; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; AAH-5039-2021
This investigation reports on 62 infrageneric taxa belonging to 38 genera from Bursa Uludag University Gorukle Campus Area. 43 of 62 taxa are new record for Gorukle Campus Area and 2 taxa are new for Bursa. A number of morphological, anatomical and ecological pecularities of the determined lichen taxa from the research area were evaluated. A great majority of the recorded lichen taxa were found as crustose and mesophytic form and low tolerance to eutrophication.
Epiphytic lichen diversity on Quercus cerris and Q. frainetto in the Marmara region (Turkey)
(TÜBİTAK, 2012) Oran, Seyhan; Öztürk, Şule; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-8196-2065; AAH-5039-2021; AAD-7322-2019; 14067648200; 7004555373
The epiphytic diversity of lichens associated with Quercus cerris L. and Quercus frainetto Ten. were investigated in 21 localities of the Marmara region. A total of 106 taxa belonging to 49 genera were reported on Q. cerris and Q. frainetto, and 16 of those taxa were recorded for the first time in the Marmara region. The number of lichen species found on Q. cerris was 83, while 85 taxa were found on Q. frainetto. Several differences regarding the lichen taxa were found in these 2 oaks species even though they were growing in the same habitat and in the same locality. Furthermore, some morphological, anatomical, and ecological peculiarities, as well as the phytogeographical patterns of the recorded lichen taxa, were evaluated. The majority of the lichen species were hygrophytic and mesophytic. In addition, nitrophytic species were more frequently observed than acidophytes. Temperate and suboceanic species were the most common.
Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro
(TÜBİTAK, 2014-08-14) Çelikler, Serap; Oran, Seyhan; Ulukaya, Engin; Aztopal, Nazlıhan; Sarımahmut, Mehmet; Öztürk, Şule; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; 0000-0003-2647-5875; 0000-0003-3118-8061; 0000-0002-6729-7908; 0000-0001-8196-2065; 0000-0003-4875-5472; AAG-8288-2021; AAV-4886-2020; K-5792-2018; AAG-7012-2021; AAD-7322-2019; AAH-5039-2021; 8234554800; 14067648200; 24376085300; 6602927353; 55853882900; 44661687400; 7004555373
Lichens are recently receiving great attention because they have potential anticancer activity. Therefore, in this study, genotoxic and antigrowth properties of lichen species Usnea filipendula Stirt. were tested against human breast cancer cell lines (MCF-7 and MDAMB-231). Antigrowth effect was assayed by the MTT and ATP viability assays. Cell death modes (apoptosis/necrosis) were evaluated morphologically (fluorescence staining) and biochemically (caspase-cleaved cytokeratin 18, caspase-3 activity, and poly-(ADP-ribose) polymerase (PARP) cleavage). Genotoxic activity of U. filipendula was determined by using micronucleus, chromosomal aberration, and comet assays in human lymphocyte culture. U. filipendula inhibited growth in a dose-dependent manner and induced apoptosis by cleavage of PARP and induction of active caspase-3. It also showed genotoxic activity in doses (125 and 250 mu g/mL) higher than that required for apoptosis. These results suggest that U. filipendula may induce apoptotic cell death at lower doses, while it may be genotoxic at higher doses.
Genotoxic, cytotoxic, and apoptotic effects of Hypogymnia physodes (L.) Nyl. on breast cancer cells
(Wiley, 2014-05) Özel, Mustafa Zafer; Özyurt, Dilek; Arı, Ferda; Çelikler, Serap; Oran, Seyhan; Balıkçı, Necmiye; Öztürk, Şule; Ulukaya, Engin; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0001-8196-2065; 0000-0002-6729-7908; 0000-0002-4177-3478; AAH-5039-2021; K-5792-2018; AAG-7012-2021; AAD-7322-2019; AAH-2767-2021; 24376085300; 8234554800; 14067648200; 55338347600; 7004555373; 6602927353
The aim of this study is to determine the chemical composition, and evaluate the genotoxic, and anti-growth potency of the methanol extracts of lichen species Hypogymnia physodes (L.) Nyl. (HPE). Anti-growth effect was tested in two different human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP viability assays and apoptosis was assayed by the caspase-cleaved cytokeratin 18 (M30-antigen). Genotoxic activity of HPE was studied using chromosome aberration and micronuclei tests in human lymphocytes culture in vitro. The chemical composition of H. physodes was analyzed by using direct thermal desorption method coupled with comprehensive gas chromatography-time of flight mass spectrometry (GCXGC-TOF/MS). Our results indicate that HPE has an anti-growth effect at relatively lower concentrations, while relatively higher concentrations are required for genotoxic activity. HPE, therefore, seems to represent a therapeutic potential and poses new challenges for medicinal chemistry.
Lecanora, phaeophyscia and rinodina species new to Turkey
(Mycotaxon, 2011) Arslan, Belgin; Öztürk, Şule; Oran, Seyhan; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; 0000-0001-8196-2065; AAD-7322-2019; AAH-5039-2021; 50661068600; 7004555373; 14067648200
Four lichenized fungi (Lecanora jamesii, L. juniperina, Phaeophyscia hirsuta, and Rinodina orculata) are reported for the first time from Turkey. Comments on their habitat, substrate, and morphological and anatomical features are provided.
Lichen records from Southeast and East Anatolian region (Turkey)
(Uludağ Üniversitesi, 2007-04-01) Oran, Seyhan; Öztürk, Şule; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
In this study, 278 lichen specimens, collected from sixteen localities in Adıyaman, Ağrı, Batman, Diyarbakır, Gaziantep, Kars, Mardin and Şanlıurfa provinces, which located in Southeast and East Anatolian regions, were investigated. Onehundered and nine taxa were determined belonging to thirty six genera. Among the specimens that we are collected from the study region, 85 of them were reported previously. Our results indicated that 24 taxa are new to Southeast and East Anatolian region. Rinodina rinodinoides (Anzi) H. Mayrhofer & Scheid. is new record for Turkey.
Lichens exerts an anti-proliferative effect on human breast and lung cancer cells through induction of apoptosis
(Taylor and Francis, 2021) Erkısa, Merve; Ulukaya, Engin; Öztürk, Şule; Oran, Seyhan; Çelikler, Serap; Arı, Ferda; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; 0000-0002-6729-7908; AAG-7012-2021; 7004555373; 14067648200; 8234554800; 24376085300
Successful cancer treatment still requires new complexes or compounds from natural sources. Therefore, we investigated anti-growth/apoptotic effects of methanol extracts of the lichen species (Xanthoparmelia somloensis (Gleyn.) Hale, Usnea intermedia (A. Massal.) Jatta, Bryoria capillaris (Ach.) Brodo & D. Hawksw and Lobaria pulmonaria (L.) Hoffm.) on human lung (A549, H1299) and breast (MCF-7, MDA-MB-231) cancer cell lines. Anti-growth effects were monitored by the MTT and ATP viability assays. Cell death mode was evaluated by employing the fluorescence staining of nucleus, caspase-cleaved cytokeratin 18 detection, caspase 3/7 activity assay, Anneksin V cytofluorimetric assay and mitochondria membrane potential assay. Among the lichen extracts, Usnea intermedia exhibited strong anti-growth activity in a dose-dependent manner (1.56-100 mu g/ml) compared to the others. Usnea intermedia was especially cytotoxic against MDA-MB-231 and H1299 cells (IC50 value for was found 3.0 and 10.2 mu g/ml respectively). The cytotoxicity was resulted from apoptosis as proved by the presence of pyknotic nuclei, caspase 3/7 activity, phosphatidylserine translocation and loss of mitochondria membrane potential. In conclusion, Usnea intermedia warrants for further in vivo evaluation as a new alternative in cancer treatment.
Marmara bölgesinde yayılış gösteren Quercus L. (meşe) ve Fagus L. (kayın) türleri üzerindeki epifitik likenlerin belirlenmesi
(Uludağ Üniversitesi, 2008) Oran, Seyhan; Öztürk, Şule; Uludağ Üniversitesi/Fen Bilimleri Enstitüsü/Biyoloji Anabilim Dalı.
Bu çalışmada, Marmara Bölgesi'nde yayılış gösteren Fagus ve Quercus taksonları üzerindeki epifitik liken çeşitliliğinin belirlenmesi ve liken türlerinin substrat tercihlerinin incelenmesi amaçlanmıştır.15.05.2005 - 06.07.2008 tarihleri arasında Marmara Bölgesi'nde Fagus ve Quercus cinslerine ait taksonların bulunduğu 238 farklı lokaliteden, 4219 adet örnek toplanmıştır. Toplanan örneklerin değerlendirilmesi ile 67 cinse ait 186 liken ve 2 likenikol mantar taksonu belirlenmiştir. Tespit edilen taksonlardan, 8'i Türkiye için yeni kayıttır. Ayrıca, 8 liken ve 2 likenikol mantar taksonu Türkiye'den ikinci kez, 19 takson ise Marmara Bölgesi'nden ilk kez kaydedilmiştir.Türkiye için yeni kayıt olan taksonlar; Anisomeridium macrocarpum (Körb.) V. Wirth, Bacidia globulosa (Flörke) Hafellner & V. Wirth, Gyalecta ulmi (Sw.) Zahlbr., Lecanora jamesii J.R. Laundon, Lithographa graphidioides (Leight.) Imshaug ex Coppins & Fryday, Opegrapha viridis Pers., Ramalina dilacerata (Hoffm.) Hoffm. ve Usnea ceratina Ach.'dır.Türkiye'den ikinci kez kayıt verilen taksonlar; Arthonia leucopellaea (Ach.) Almq., Arthonia punctiformis Ach., Arthonia stellaris Kremp., Dactylospora parasitica (Flörke ex Spreng.) Zopf, Lecanora impudens Degel., Micarea melaena (Nyl.) Hedl., Ochrolechia subviridis (Høeg) Erichsen, Physconia servitii (Nádv.) Poelt, Ramalina breviuscula Nyl. ve Stigmidium congestum (Körb.) Triebel'dur.Çalışma alanında bulunan substratlar ve bunlar üzerinden tespit edilen epifitik takson sayıları ise şu şekildedir; Quercus cerris L. 145, Q. frainetto Ten. 119, Q. petraea (Mattuschka) Liebl. 102, Q. pubescens Willd. 91, Fagus oreintalis Lipsky 79, Q. virgiliana Ten. 47, Q. infectoria Olivier 42, Q. trojana P. B. Webb 42, Q. ithaburensis subsp. macrolepis (Kotschy) Hedge 30, Q. robur L. 24, Q. coccifera L. 21, F. sylvatica L. 8 ve Q. hartwissiana Steven 7.Çalışma kapsamında yer alan ve epifitik liken türlerinin substratını oluşturan Fagus ve Quercus taksonlarının kabuk pH'ları ölçülmüştür. Belirlenen pH değerlerine göre liken çeşitliliğinde gözlenen farklılıklar değerlendirilmiştir. Q. cerris'in en düşük (pH 4,76), Q. frainetto'nun ise en yüksek (pH 6,16) ortalama pH değerine sahip olduğu saptanmıştır. Asit özellikteki kabukların asidofil liken taksonlarına ev sahipliği yaptığı, çeşitli nedenlerden dolayı ortaya çıktığı düşünülen, kabuk pH'ındaki artışın substratları nitrofil liken taksonları için uygun hale getirdiği gözlenmiştir.
Parmelia sulcata Taylor and Usnea filipendula Stirt induce apoptosis-like cell death and DNA damage in cancer cells
(Wiley, 2014-10) Arı, Ferda; Aztopal, Nazlıhan; Oran, Seyhan; Bozdemir, Şefika; Çelikler, Serap; Öztürk, Şule; Ulukaya, Engin; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-6729-7908; 0000-0001-8196-2065; 0000-0002-4177-3478; 0000-0003-3118-8061; AAG-7012-2021; AAD-7322-2019; AAH-5039-2021; K-5792-2018; AAH-2767-2021; L-6687-2018; 24376085300; 55853882900; 14067648200; 57506388900; 8234554800; 7004555373; 6602927353
Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti-proliferative/apoptotic effects of methanolic extracts of lichen species Parmelia sulcata Taylor and Usnea filipendula Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells. Materials and methodsAnti-proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase-cleaved cytokeratin 18, caspase-3 activity and PARP cleavage) were performed. ResultsExtracts produced anti-population growth effects in a dose-dependent manner (1.56-100g/ml) by inducing apoptosis-like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death. ConclusionIn this study, methanolic extracts of Parmelia sulcata and Usnea filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.
Promising anticancer activity of a lichen, Parmelia sulcata Taylor, against breast cancer cell lines and genotoxic effect on human lymphocytes
(Springer, 2015-05-01) Özel, Mustafa Zafer; Arı, Ferda; Ulukaya, Engin; Oran, Seyhan; Celikler, Serap; Ozturk, Sule; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-6729-7908; 0000-0003-4875-5472; 0000-0001-8196-2065; 0000-0002-4177-3478; 0000-0002-1284-7992; AAG-7012-2021; K-5792-2018; AAH-5039-2021; AAH-2767-2021; AAD-7322-2019; 24376085300; 6602927353; 14067648200; 8234554800; 7004555373
Plants are still to be explored for new anticancer compounds because overall success in cancer treatment is still not satisfactory. As a new possible source for such compounds, the lichens are recently taking a great attention. We, therefore, explored both the genotoxic and anti-growth properties of lichen species Parmelia sulcata Taylor. The chemical composition of P. sulcata was analyzed with comprehensive gas chromatography-time of flight mass spectrometry. Anti-growth effect was tested in human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP viability assays, while the genotoxic activity was studied by assays for micronucleus, chromosomal aberration and DNA fragmentation in human lymphocytes culture. Cell death modes (apoptosis/necrosis) were morphologically assessed. P. sulcata inhibited the growth in a dose-dependent manner up to a dose of 100 mu g/ml and induced caspase-independent apoptosis. It also showed genotoxic activity at doses (>125 mu g/ml) higher than that required for apoptosis. These results suggest that P. sulcata may induce caspase-independent apoptotic cell death at lower doses, while it may be genotoxic at relatively higher doses.
Ramalina lichens and their major metabolites as possible natural antioxidant and antimicrobial agents
(Wiley-Hindawi, 2015-08-01) Şahin, Saliha; Oran, Seyhan; Şahintürk, Pınar; Demir, Cevdet; Öztürk, Şule; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Bölümü.; 0000-0001-8196-2065; AAH-2892-2021; AAH-5039-2021; JJA-0642-2023; DTQ-5753-2022; AAD-7322-2019; 15027401600; 14067648200; 55342852700; 7003565902; 7004555373
Three lichen species of Ramalina (R.farinacea, R.fastigiata and R.fraxinea) were examined. Evernic, fumarprotocetraric, lecanoric, stictic and usnic acid levels were determined by high performance liquid chromatography-diode array detection. Acetone, methanol and ethanol were used to examine the efficiencies of different solvent systems for the extraction of lichen acids. The total phenol contents in the extracts were determined by the Folin-Ciocalteu method. The antioxidant capacities were determined by the ABTS (2,2-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid]) method. The methanol extracts of the Ramalina species showed the highest antioxidant capacities. Broth microdilution testing was performed to determine the minimum inhibitory concentration (MIC) of the methanol extracts of the three Ramalina species. The MIC values of all extracts ranged from 64 to 512g/mL for all bacterial strains tested in this study. Practical ApplicationsLichens and their natural products are used worldwide for decorations, brewing and distilling, food, fodder, spice and natural remedies, and in the perfume and dying industries. Lichens produce a large number of phenolic compounds, such as depsides, depsidones and dibenzofurans. Lichens with antioxidant activity have increased abilities to scavenge toxic-free radicals due to their phenolic groups. In recent years, many lichen substances have been found to have several biological activities. This article evaluates the antimicrobial and antioxidant activities and lichen acids of three Ramalina species. This is the first study to determine the stictic acid level in a R.farinacea extract and fumarprotocetraric acid and lecanoric acid levels in an R.fastigiata extract. The results of this study will contribute significantly to current knowledge regarding the utility of antimicrobial and antioxidant materials.
Some lichen records from Quercus vulcanica forests around Yukarı Gökdere (Isparta, Turkey)
(Uludağ Üniversitesi, 2007-12-01) Oran, Seyhan; Uğur, Ayşegül; Öztürk, Şule; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
The lichen flora of the Quercus vulcanica forests in Isparta was studied and 92 species belonging to 43 genera were found. 55 lichen taxa are new records for the Isparta province and 2 taxa, Ochrolechia alboflavescens and Collema fasciculare are new records for Turkey.
The determination of the changes in epiphytic lichen diversity at microclimatic conditions the Quercus petraea (Mattuschka) Liebl. forest in the Uludag Mountains (Bursa, Turkey)
(Gebruder Borntraeger, 2019-11-01) Öztürk, Sule; ÖZTÜRK, ŞULE; Güvenç, Şaban; GÜVENÇ, ŞABAN; Oran, Seyhan; ORAN, SEYHAN; 0000-0001-8724-9981; AAD-7322-2019; AAG-7065-2021; AAH-5039-2021
The aim of this study is to determine the effects of microclimatic factors and tree size on epiphytic lichen diversity in different parts of Quercus petraea. The microclimatic factors used were tree size, the base and trunk of the tree. bark surface moisture, and environmental factors such as the amount of relative humidity and light in the environment. A total of 35 epiphytic lichen species was found in the Quercus petraea forest in the Uludag Mountains. Parmelia sulcata was the most common species. The highest species diversity was found in small trees. Shannon diversity on the trunk of the tree decreased with the increase in the tree size. The relationship between tree size and species richness on the trunk of the tree was found to be more significant than that found on the base. It has been determined that the frequency of some species on the base and trunk of the tree was increased as a result of an increase in the amount of relative humidity and light in the environment and an increase in the bark surface moisture. At the same time the frequency decreased when the amount of relative humidity and light in the environment and bark surface moisture decreased.
Three lichenized fungi new to Turkey
(Mycotaxon, 2010) Oran, Seyhan; Öztürk, Şule; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-8196-2065; AAD-7322-2019; AAH-5039-2021; 14067648200; 7004555373
In this study, three lichenized fungi (Gyalecta ulmi, Ochrolechia subviridis and Opegrapha viridis) are reported for the first time from the Turkish provinces of Canakkale, Istanbul and Kirklareli. Comments on their habitat and substrata and a short description are provided for each taxon.
Usnea filipendula induces apoptosis in human colon cancer cell lines
(Indian Pharmaceutical Assocation, 2018-10-05) Disoma, Cyrollah; Erkısa, Merve; Ulukaya, Engin; Oran, Seyhan; Alioğlu, İmren; Arı, Ferda; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü; 0000-0001-8196-2065; 0000-0002-6729-7908; AAH-5039-2021; AAG-7012-2021; 14067648200; 57277624900; 24376085300
Lichens are complex organisms living in a symbiotic relationship with fungi and algae have recently received special interest in cancer research. The cytotoxic activities of Usnea filipendula Stirt. lichen extract was investigated on colon cancer cell lines, HCT-15 and HT-29. Sulphorhodamine B and ATP cell viability tests were used to monitor cytotoxic activity. The mode of cell death (apoptosis/necrosis) was determined using caspase-cleaved cytokeratin 18 (M30), caspase-3/7 activity and fluorescence staining techniques that included, Annexin-V, Hoechst 33342 and propidium iodide. Usnea filipendula showed dose and time-dependent antiproliferative effect in HCT-15 and HT-29 cells. The IC50 values in HCT-15 and HT-29 cells were 17.92 and 41.87 mu g/ml, respectively. The extract induced apoptosis in both cell lines especially in HCT-15 cells in which caspase-3/7 activity was increased. Usnea filipendula was cytotoxic to colon cancer HCT-15 and HT-29 cell lines by inducing early or late apoptosis as evidenced by translocation of phosphatidylserine, pyknotic nuclei and nuclear condensation. Further studies would help to understand the full potential of Usnea filipendula as a novel anticancer therapy.