Browsing by Author "Rostami, Ahmad Ali"
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Item In vitro micropropagation of olive (Olea europaea L.) ‘Mission’ by nodal segments(Uludağ Üniversitesi, 2012) Rostami, Ahmad Ali; Shahsavar, AliOlive (Olea europaea L.) cultivars are mainly propagated by hardwood cuttings under mist unit of greenhouses. Such techniques are time consuming, laborious and have limited efficiency. In vitro propagation methods may be a good alternative for propagation of olive cultivars. Current study was conducted to establish a successful and high efficient method for micropropagation of olive ‘Mission’ via nodal segments. Results of this study showed that production of phenolic compounds and necrosis of explants may be controlled by submersing the explants in a mixture of 100 mg L-1 citric acid and ascorbic acid for 30 minutes, after submersion in water for 2 hours. The best proliferation rate and growth obtained in the presence of benzyladenine and gibberellic acid at the rate of 2.1, 2.08, and 0.6 mg L-1, respectively. Proliferated explants rooted on MS/2 supplemented with 4 mg L-1 indolbutyric acid. Rooted explants adapted to outdoor condition by placing them under ordinary mist/cooling unit system of a greenhouse. The method described in this study is suitable for bulk propagation of olive ‘Mission’ in a period of three to four months; however the applicability of the method should be evaluated for other olive cultivars.Item Responses of caprifig genotypes to water stress and recovery(Uludağ Üniversitesi, 2013-10-21) Rostami, Ahmad Ali; Majid, RahemiCaprifig (Ficus carica L.) as pollen sources play a major role in fig breeding programs. The aim of this study was to evaluate responses of four caprifig genotypes (‘Dane Sephid’, ‘Pouz Donbali’, ‘Shah Anjiri’, and ‘Khormaei’) to water stress and rewatering cycles. Water stress was applied to one-year-old caprifig cuttings by withholding irrigation for 14 days and was followed by a 10-day rewatering period. Growth parameters of the genotypes were significantly reduced under water stress period. Results indicated that water stress significantly reduced relative water content (RWC) and leaf water potential (ΨLeaf), and the lowest values were found in ‘Dane Sephid’. Electrolyte leakage increased in parallel to decrease of leaf RWC and ΨLeaf, and EL was significantly higher in the leaves of ‘Dane Sephid’. On the contrary to inorganic osmolytes, water stress enhanced proline accumulated in the leaves of caprifig genotypes with the exception to ‘Dane Sephid’. After the rewatering period growth indices of ‘Khormaei’ and ‘Shah Anjiri’ were recovered to the control level. The data suggested 'Khormaei' and 'Shah Anjiri' have higher drought tolerance. The mechanism underlying the drought tolerance in caprifigs may result from their capacity of osmoregulation and maintaining cell health status.