Browsing by Author "Rumbeiha, Wilson K."

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Simultaneous detection of six different groups of antimicrobial drugs in milk, meat, urine, and feed matrices
(Uludağ Üniversitesi, 2013-01-29) Rumbeiha, Wilson K.; Ensley, Steve; Schrunk, Dwayne E.; Oruç, Hasan H.; Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Bölümü.
Antimicrobials are widely used for animal health in food-producing animals. Therefore, antimicrobial residues are of food safety concern. Simultaneous detection of different antimicrobial residues in the same matrice is important. Therefore, the objective of this study was to detect six different group antimicrobials in milk, meat, urine, and feed matrices under experimental conditions, and to evaluate its use for routine analysis. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different groups of six antimicrobials in milk, urine, meat, honey, and feed. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in milk, meat, urine, and in feed matrices. In conclusion, this assay can effectively detect all target antibacterials from different groups in milk, meat, urine, and feed matrices, and this assay can be used for routine detection of these antibacterial residues in the stated matrices.
Simultaneous detection of six different groups of antimicrobial drugs in porcine oral fluids using a biochip array-based immunoassay
(Kafkas Üniversitesi, 2013) Rumbeiha, Wilson K.; Ensley, Steve; Olsen, Chris; Schrunk, Dwayne E.; Oruç, Hasan Hüseyin; Uludağ Üniversitesi/Veterinerlik Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.; 0000-0002-5399-2395; AAI-2212-2021; 55944769400
The objectives of this study were 1) to determine whether a biochip array-based immunoassay could be used to detect 6 different group antimicrobials in porcine oral fluids collected under experimental conditions; 2) to determine the feasibility of antimicrobial detection in clean versus dirty oral fluid samples; and 3) to determine if the assay could be used to detect chlortetracycline in oral fluids of swine ingesting a routine diet containing chlortetracycline. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different group six antimicrobials in milk, urine, meat, honey, and feed. The assay had not been investigated for potential use in oral fluids. Following evaluation of different extraction procedures, a centrifugation method was chosen. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in both clean and dirty oral fluid samples. However, tetracycline was not well recovered in dirty samples. Chlortetracycline from tetracycline group was detected in all oral fluid samples collected from the field at concentrations ranging from 176.0-698.6 ppb. In conclusion, this assay can detect all target antibacterials from different groups in clean and dirty oral fluid samples with the exception of tetracycline which was not well-recovered from in dirty samples.