Browsing by Author "Sarandöl, Emre"
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Item Adiponectin, leptin, nitric oxide, and C-reactive protein levels in kidney transplant recipients: Comparison with the hemodialysis and chronic renal failure(Taylor & Francis, 2016-08-20) Ocak, Nihal; Dirican, Melahat; Ersoy, Alparslan; Sarandöl, Emre; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Nefroloji Anabilim Dalı.; 0000-0002-0710-0923; 0000-0002-2593-7196; AAH-5054-2021; ABE-1716-2020; 23989248600; 6601919847; 35612977100; 55943324800Background: Cardiovascular disease (CVD) is the leading cause of mortality and morbidity in patients with chronic kidney disease (CKD) including kidney transplant recipients (KTR). Secondary lipid metabolism disorders, endothelial dysfunction, and inflammation enhance the risk of CVD development in these patients. The aim of the present study was to investigate the lipid profile, adiponectin, leptin, nitric oxide (NO), and high sensitivity C-reactive protein (hs-CRP) levels in KTR and to compare these parameters with those of the patients with chronic renal failure (CRF), hemodialysis (HD) patients, and healthy controls.Methods: Serum adiponectin and leptin levels were measured by radioimmunoassay; hs-CRP was determined immunoturbidimetrically. Determination of NO was based on the Griess reaction.Results: Compared with the control group, serum NO and adiponectin levels were significantly higher in the KTR, CRF, and HD groups; hs-CRP levels were significantly higher in the KTR and HD groups; leptin levels were significantly higher in the KTR. In addition, serum NO level was significantly higher in the KTR compared to CRF cases. Adiponectin correlated positively with high density lipoprotein-cholesterol in the control and patient groups. A positive correlation was observed between hs-CRP and NO in the KTR and the patients with CRF. Serum adiponectin levels were inversely correlated with hs-CRP and leptin in the HD group.Conclusion: KTR suffer from inflammation and accompanying changes in levels of adipocytokines and NO which contribute to the increased risk of CVD in these patients.Item Anti-inflammatory efficiency of levobupivacaine in an experimental colitis model(Baishideng Publishing Group, 2010-05-28) Duman, Uğur; Yılmazlar, Aysun; Öztürk, Ersin; Aker, Sibel; Sarandöl, Emre; Yılmazlar, Tuncay; Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Anesteziyoloji ve Reanimasyon Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; 0000-0002-4946-555X; ABE-1716-2020; H-5770-2018; 36971700700; 55899579900; 35070171400; 12795285000; 55943324800; 6701800362AIM: To investigate the efficiency of levobupivacaine in treating experimentally induced colitis in rats. METHODS: Colitis was induced by trinitrobenzene sulfonic acid and ethanol in 30 rats under general anesthesia, and 10 rats were used as a sham group. Subsequent to induction of colitis, rats were divided into three groups; budesonide group received 0.1 mg/kg budesonide, levobupivacaine group received 10 mg/kg levobupivacaine and saline group received 1 mL saline solution via rectal route for 7 d. In the sham group, only routine rectal catheterization was performed without use of any material. At the end of 7 d, laparotomy and total colectomy were performed for histopathological examination in all rats and blood samples were drawn for measurement of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 following cardiac puncture. Macroscopic and microscopic evaluations of the specimens were performed by a pathologist blinded to group assignment of the rats. RESULTS: Weight loss (P = 0.016) and macroscopic examination scores (P = 0.001) were significantly higher in saline group than others. Histopathological scoring was comparable between all colitis groups (P = 0.350). There was no significant difference in TNF-alpha levels and IL-6 levels (P = 0.150). CONCLUSION: The significant improvement in macroscopic scores suggests that levobupivacaine may have topical anti-inflammatory effects in an experimental colitis model; however, this finding was not supported by microscopic findings.Item Assessing the impact of insulin glargine and detemir treatment to serum total IGF1 levels in the insulin-naive type 2 diabetic patients(Mary Ann Liebert, 2017-06) Dizdar, Oğuzhan Sıtkı; Koca, Nizameddin; Aydın, Taner; Cander, Soner; Gül, Özen Öz; Sarandöl, Emre; Ersoy, Canan; Uludağ Üniversitesi/Tıp Fakültesi/Endokrinoloji ve Metabolizma Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; AAI-1005-2021; ABE-1716-2020; AAH-8861-2021; 25027068600; 26040787100; 55943324800; 6701485882Aim: The mitogenic potential of analog insulins due to their different insulin-like growth factor-1 (IGF1) receptor affinity is a situation that causes concern related to cancer risk. We aimed to examine the changes in the serum IGF1 levels formed by insulin glargine and detemir in the insulin-naive type 2 diabetic patients. Methods: The serum total IGF1 levels of the 62 insulin-naive type 2 diabetic patients were studied before and after 12 weeks of the started treatment with basal insulin analogs. Twenty-two and twenty patients (Group I and II) using the single-dose and double-dose insulin detemir and twenty patients (Group III) using insulin glargine were evaluated. Results: In Group I and Group II, the average 8.5% and 0.1% increases and in the Group III, 6.5% decreases were determined in the IGF1 values. The IGF1 changes were significant in the men but not in the women. Conclusion: In our study, it was determined that the insulin glargine depressed the serum IGF1 levels much more when compared to the insulin detemir. This result can be evaluated as the in vivo reflection of the in vitro findings related to the fact that the IGF1 receptor affinity of the glargine is higher.Item The association between cardiac valvular calcification and fetuin-A levels in kidney transplant recipients(Springer, 2019-06-18) Koca, Nizameddin; Şensoy, Barış; Kırhan, Emine; Ersoy, Alparslan; Güllülü, Sümeyye; Ersoy, Canan; Dirican, Melahat; Sarandöl, Emre; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Nefroloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Kardiyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Endokrinoloji ve Metabolizma Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-0710-0923; 0000-0002-2593-7196; AAH-5054-2021; JGR-6552-2023; AAH-8861-2021; ESK-6562-2022; ABE-1716-2020; 35612977100; 57204660708; 6701485882; 6601919847; 55943324800Objectives Low fetuin-A levels in hemodialysis patients can be associated with development of vascular and valvular calcifications. The mechanisms underlying vascular and valvular calcifications are multifactorial. There are a few studies showing the relationship between low fetuin-A levels and valvular calcification after kidney transplantation. We aimed to evaluate the association between serum fetuin-A levels and valvular calcification in kidney transplant recipients. Methods The cardiac valvular calcification was assessed by echocardiography in 56 recipients. Patients were divided into two groups as those with (n = 11) and without (n = 45) aortic and/or mitral valve calcification. The extent of valvular calcification was visually assessed according to the standard visual score method: moderately (multiple larger spots) and heavily calcified (extensive thickening and calcification) of all cusps. Serum fetuin-A levels were measured. Results The demographic features of both groups were comparable. There was no significant difference between regular physical exercise (63.6% vs. 55.6%), obesity (18.2% vs. 17.8%), abdominal obesity (54.5% vs. 46.7%), smoking (0% vs. 13.3%), hypertension (63.6% vs. 68.9%), left ventricular hypertrophy (45.5% vs. 33.3%) and diabetes mellitus (9.1% vs. 20%) ratios in groups with or without valvular calcification, respectively (p > 0.05). Fetuin-A levels of both groups did not differ. Fetuin-A levels positively correlated with serum creatinine (r 0.326, p = 0.014), and negatively correlated with estimated glomerular filtration rate (r - 0.297, p = 0.026). Conclusions We could not find a relationship between serum fetuin-A levels and valvular calcification in kidney recipients. In this population, further studies are needed to assess the role of serum fetuin-A in valvular calcification.Item Association between paraoxonase 1 activity and severity of coronary artery disease in patients with acute coronary syndromes(Taylor & Francis, 2008-06) Şentürk, Tunay; Sarandöl, Emre; Güllülü, Sümeyye; Erdinç, Selda; Özdemir, Bülent; Baran, İbrahim; Aydınlar, Ali; Uludağ Üniversitesi/Tıp Fakültesi/Kardiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; 0000-0002-8974-8837; C-1517-2017; ABE-1716-2020; AAI-6632-2021; 8342098300; 55943324800; 57204660708; 24334883200; 7004168959; 35572557400; 6603131517Objective - We sought to investigate serum paraoxonase/arylesterase activities in patients with acute coronary syndromes (ACS) and their correlations with the severity and extent of coronary artery disease (CAD). Methods and results - Three groups of patients were investigated: 89 patients with ACS, 54 patients with normal coronary angiograms (no-CAD group), and 27 healthy comparison subjects. ACS patients were divided into three groups according to their clinical presentation: unstable angina pectoris (UAP Braunwald Ill-B, n = 31), non-ST elevation myocardial infarction (NSTEMI) (n = 27), and ST-elevation myocardial infarction (STEMI) (n = 31). Serum paraoxonase/arylesterase activities were measured spectrophotometrically. Angiographic CAD extent was expressed both by the number of vessels diseased and by the Gensini scoring system. Results showed that serum paraoxonase/ arylesterase activities and the paraoxonase/high density lipoprotein-cholesterol (HDL-C) ratio were significantly lower in the STEMI, NSTEMI, UAP groups than in no-CAD and control groups. Serum paraoxonase/arylesterase activities and paraoxonase/HDL-C ratio were reduced in patients with 2-vessel disease (VD) and 3-VD compared to the I-VD and no-CAD group (P<0.001). In patients with ACS, the Gensini score correlated inversely with serum paraoxonase (r = -0.419, P< 0.001), arylesterase (r = -0.492, P< 0.0001), and the paraoxonase/HDL-C ratio (r = -0.377, P< 0.001). Serum arylesterase (r = 0.161, P = 0.03) and paraoxonase (r = 0.135, P = 0.002) activities were positively correlated with HDL-C levels. Serum arylesterase activity (P< 0.0001), gender (P = 0.0037), diabetes mellitus (P = 0.005) and LDL-C levels (P = 0.03) were independent predictors of CAD presence. Conclusions - Serum paraoxonase/arylesterase activities are reduced in ACS patients and inversely correlated with the severity of CAD.Item Body fat distribution has no effect on serum visfatin levels in healthy female subjects(Academic Press - Elsevier Science, 2010-03) Ersoy, Canan; Sadıkoğlu, Ganime; Orhan, Hasan; Güçlü, Metin; Sarandöl, Emre; Akgün, Meral Demirci; Özçakır, Alis; İmamoğlu, Şazi; Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Aile Hekimliği Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; 0000-0001-5588-2037; ABI-4847-2020; ABE-1716-2020; AAG-8209-2021; AAH-8861-2021; 6701485882; 22956348700; 36126806400; 15073842600; 55943324800; 36125941900; 9733230000; 6602297533Obesity is the presence of either abnormal absolute amount or relative proportion of body fat. Contrary to gluteal obesity, visceral obesity is associated with different metabolic alterations including insulin resistance (IR). A relatively new adipocytokine visfatin is shown to be expressed predominantly in visceral fat and exhibit insulin-mimicking effects in rodents. It is still unclear whether serum visfatin levels are associated with increased total or visceral fat mass in humans. The aim of our study was to investigate the relation between visfatin and obesity parameters namely body mass index (BMI) and waist circumference (WaC) and IR in healthy female subjects. Eighty one female subjects >= 20 years of age, having no diagnosis of glucose intolerance or diabetes, hypertension and dyslipidemia were chosen. The patients were divided into four groups according to their BMI and WaC values. Serum visfatin and HOMA-IR levels did not differ among groups. No correlation was detected between serum visfatin levels and obesity and metabolic parameters. In conclusion, we demonstrated that body fat distribution did not affect serum visfatin levels in healthy female subjects. Further studies are needed to clarify the exact factors influencing and determining serum visfatin levels and its clinical reflections.Item Can BDNF and IL-2 be indicators for the diagnosis in schizophrenic patients with depressive symptoms?(Cambridge University, 2014-10) Cangur, Şengül; Eker, Salih Saygın; Yavaşçı, Ebru Öztepe; Kırlı, Selçuk; Sarandöl, Emre; Uludağ Üniversitesi/Tıp Fakültesi/Psikiyatri Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; ABE-1716-2020; Q-9477-2019; 14019347700; 56182922000; 14019745700; 55943324800Objective: The aim of the current study is to determine whether serum levels of brain-derived neurotrophic factor (BDNF) and interleukin-2 (IL-2) can be biological indicators for the diagnosis of schizophrenia in patients with depressive symptoms. Method: Forty-seven patients (11 patients diagnosed with schizophrenia, 16 patients diagnosed with schizophrenia and comorbid depression and 20 patients diagnosed with major depressive disorder) and 20 healthy subjects were enrolled. The Positive and Negative Symptoms Scale, the Calgary Depression Scale for Schizophrenia and the Hamilton Depression Rating Scale were used for assessment. The serum BDNF and IL-2 levels of all the subjects were studied. Results: Decreased levels of serum BDNF and increased levels of serum IL-2 were found in the patients diagnosed with either schizophrenia, schizophrenia with depression, or major depressive disorder (p = 0.049, p = 0.010; p = 0.001 and p = 0.044; p = 0.027, p = 0.003; respectively) compared with control group. There were no significant differences between the patient groups in their serum BDNF and IL-2 levels. Conclusions: The present study suggests that neurotrophic factors and immune system changes are involved in the pathogenesis of schizophrenia with or without depressive symptomatology. However, the data do not clarify whether depressive symptoms in schizophrenia occur as a dimension of schizophrenia or as symptoms of major depression that is comorbid with schizophrenia.Item The comparison of effects of calcineurin inhibitors on cytokines and TGF-beta in kidney transplant recipients(Oxford University Press, 2013-05) Koca, Nizameddin; Koca, Tuba Güllü; Ersoy, Alparslan; Kırhan, Emine; Ersoy, Canan; Sarandöl, Emre; Dirican, Melahat; Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0002-0710-0923; 0000-0002-2593-7196; GWQ-5007-2022; AAH-5054-2021; AAG-6985-2021; ABE-1716-2020; AAH-8861-2021Item Coronary artery disease risk factors in patients with schizophrenia: Effects of short term antipsychotic treatment(Sage Publications, 2007-11) Sarandöl, Aslı; Kirli, Selçuk; Akkaya, Cengiz; Ocak, Nihal; Erol, Esma; Sarandöl, Emre; Uludağ Üniversitesi/Tıp Fakültesi/Psikiyatri Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; ABE-1716-2020; 14020405100; 14019745700; 14061855100; 23989248600; 22950749100; 55943324800The aim of the present study was to investigate serum paraoxonase/ arylesterase activities and oxidation/ oxidizability of apolipoprotein B- containing lipoproteins and several coronary artery disease risk factors, including homocysteine, high sensitive C- reactive protein, tumour necrosis factor- alpha, leptin and adiponectin in patients with schizophrenia. Oxidation of lipoproteins plays an important role in atherogenesis, and the enzyme paraoxonase has been shown to prevent lipoprotein oxidation. Furthermore, low paraoxonase activity has been suggested to predict coronary artery disease. Forty patients who fully met the fourth Diagnostic and Statistical Manual of Mental Disorders criteria for schizophrenia and 35 healthy control subjects were included in the study. Serum paraoxonase/ arylesterase activities were determined spectrophotometrically. Malondialdehyde levels of apolipoprotein B- containing lipoproteins were determined before and after incubation with copper- sulphate, which yielded basal- and Delta- malondialdehyde values, respectively. Homocysteine and highly sensitive C- reactive protein levels were determined using a fluorescence- polarization immunoassay and immunonephelometry, respectively. Leptin and adiponectin levels were measured with radioimmunoassay and tumour necrosis factor-alpha was determined by enzyme linked immunosorbent assay. Serum paraoxonase and arylesterase activities were significantly lower and Delta- malondialdehyde levels were significantly higher in the schizophrenia group compared with the control group. However, there were not any significant differences in other parameters of the study between the study groups. There was a significant increase in body mass index and serum triglyceride and very low density lipoprotein cholesterol levels in the schizophrenic group after 6 weeks of treatment. These parameters were significantly increased in patients treated with atypical antipsychotics but not in patients treated with typic or long acting antipsychotics. The results of the present study suggest that patients with schizophrenia might have increased risk for coronary artery disease related to reduced serum paraoxonase activity and increased oxidizability of apolipoprotein B- containing lipoproteins.Item The effect of calcineurin inhibitors on adipocytokines and neck circumference in kidney transplant recipients(Oxford University Press, 2013-05) Koca, Nizameddin; Koca, Tuba Güllü; Ersoy, Alparslan; Kirhan, Emine; Sarandöl, Emre; Ersoy, Canan; Dirican, Melahat; Uludağ Üniversitesi/Tıp Fakültesi/İç Hastalıkları Anabilim Dalı.; 0000-0002-2593-7196; 0000-0002-0710-0923; AAH-8861-2021; ABE-1716-2020; AAH-5054-2021; GWQ-5007-2022Item Effect of high fructose corn sirup on pancreatic ductal adenocarcinoma induced by dimethyl benzantracene (DMBA) in rats(Routledge Journals, 2020-05-12) İnan, Sevda; Akgül, Mustafa Barış; Bağdaş, Deniz; Sinir-Özcan, Gülşah; Suna, Senem; Tamer, Canan Ece; Sönmez, Gürsel; Evrensel, Türkkan; Kaya, Ekrem; Sarandöl, Emre; Dündar, Halit Ziya; Tarım, Ömer Faruk; Ercan, İlker; Sığırlı, Deniz; İncedayı, Bige; Çopur, Ömer Utku; Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Onkoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Endokrinolojisi Anabilim Dalı.; 0000-0003-3954-0058; 0000-0002-6947-2167; 0000-0003-0441-1707; 0000-0003-1840-1825; 0000-0002-9732-5340; 0000-0002-9562-4195; 0000-0002-2593-7196; 0000-0002-7346-7440; 0000-0002-5322-5508; 0000-0002-2382-290X; 0000-0001-5871-8769; 0000-0001-6128-7453; 0000-0002-1951-7937; AAG-8277-2021; ABE-1716-2020; AAQ-8178-2020; AAJ-1027-2021; AAG-7319-2021; AAG-8503-2021; AAG-8336-2021; AAA-7472-2021; ABF-2367-2020; 57194441710; 55512747500; 8228159500; 55167435000; 6603942124; 7004568109; 55943324800; 55453773300; 6701427186Increased risk of pancreatic cancer may be associated with consumption of sugar containing foods. The aim of this study was to evaluate the effect of peach nectar containing high fructose corn sirup (HFCS) consumption in a pancreatic carcinogenesis rat model induced by 7,12-Dimethyl benzanthracene (DMBA). Fifty-day-old male Sprague Dawley rats were fed with peach nectar containing HFCS + chow, peach nectar containing sucrose + chow and only chow. After 8 mo, feeding period, each group was divided into two subgroups, in which the rats were implanted with DMBA and no DMBA (sham). Histologic specimens were evaluated according to the routine tissue processing protocol. The animals withad libitumaccess to pn-HFCS, pn-sucrose and chow (only) showed significant differences in chow consumption and glucose level. Necropsy and histopathologic findings showed tumor formation in the entire group treated with DMBA. Excluding one rat in chow group, which was classified as poorly differentiated type, the others were classified as moderately differentiated pancreatic ductal adenocarcinoma (PDAC). This study demonstrated that daily intake of HFCS did not increase body weight and there was no effect of peach nectar consumption on the development of PDAC induced by DMBA in rats.Publication Effect of rosiglitazone and insulin combination therapy on inflammation parameters and adipocytokine levels in patients with Type 1 DM(Hindawi, 2015-03-17) Güçlü, Metin; Gül, Özen Öz; Cander, Soner; Ünal, Oğuzkaan; Özkaya, Güven; Sarandöl, Emre; Ersoy, Canan; Güçlü, Metin; ÖZ GÜL, ÖZEN; CANDER, SONER; Ünal, Oğuzkaan; ÖZKAYA, GÜVEN; SARANDÖL, EMRE; ERSOY, CANAN; Uludağ Üniversitesi/Tıp Fakültesi/Endokrinoloji ve Metabolizma Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0001-5082-9894; 0000-0003-0297-846X; A-4421-2016; ABE-1716-2020; AAI-1005-2021; ABI-4847-2020; AAH-8861-2021; HUR-0563-2023; IPU-7626-2023Aim. To investigate the efficacy of combined therapy of insulin and rosiglitazone on metabolic and inflammatory parameters, insulin sensitivity, and adipocytokine levels in patients with type 1 diabetes mellitus (type 1 DM). Material and Methods. A total of 61 adults with type 1 DM were randomly and prospectively assigned in open-label fashion to take insulin and rosiglitazone 4mg/day (n - 30) or insulin alone (n = 31) for a period of 18 weeks while undergoing insulin therapy without acute metabolic complications. Results. Combination therapy did not significantly improve metabolic and inflammatory parameters, insulin sensitivity, and adiponectin levels. While leptin and resistin levels decreased in both groups (group 1: resistin 6.96 +/- 3.06 to 4.99 +/- 2.64, P = 0.006; leptin 25.8 +/- 17.6 to 20.1 +/- 12.55, P = 0.006; group 2: resistin 7.16 +/- 2.30 to 5.57 +/- 2.48, P = 0.031; leptin 16.72 +/- 16.1 to 14.0 +/- 13.4, P = 0.007) Hgb and fibrinogen levels decreased only in group 1 (Hgb 13.72 +/- 1.98 to 13.16 +/- 1.98, P = 0.015, and fibrinogen 4.00 +/- 1.08 to 3.46 +/- 0.90, P = 0.002). Patients in both groups showed weight gain and the incidence of hypoglycemia was not lower. Discussion. The diverse favorable effects of TZDs were not fully experienced in patients with type 1 DM. These results are suggesting that insulin sensitizing and anti-inflammatory characteristics of TZDs were likely to be more pronounced in patients who were not totally devoid of endogenous insulin secretion.Item Effect of sitagliptin monotherapy on serum total ghrelin levels in people with type 2 diabetes(Elsevier Ireland, 2011-11) Gül, Özen Öz; Kıyıcı, Sinem Kücçuüksaraç; Ersoy, Canan; Cander, Soner; Yorulmaz, Hakan; Gül, Cuma Bülent; Sarandöl, Emre; Kırhan, Emine; Sığırlı, Deniz; Ertürk, Erdinç; Tuncel, Ercan; İmamoğlu, Sazi; Ünal, Oğuz Kağan; Uludağ Üniversitesi/Tıp Fakültesi/Endokrinoloji ve Metabolizma Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Dahiliye Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0003-2467-9356; 0000-0002-2593-7196; AAI-1005-2021; A-7063-2018; AAJ-6536-2021; AAA-7472-2021; ABE-1716-2020; AAH-8861-2021; 26040787100; 12753880400; 6701485882; 25027068600; 24438635700; 23988796000; 55943324800; 37104411100; 24482063400; 7005488796; 7006929833; 55042241400Aim: Sitagliptin is not associated with weight gain and has neutral effects on body weight. It is unclear whether sitagliptin treatment alters serum ghrelin levels in people with type 2 diabetes. Methods: Forty-four subjects with type 2 diabetes were randomly assigned to receive sitagliptin or medical nutrition therapy (MNT) for 12 weeks. Changes in anthropometric variables, glycemic control, insulin resistance, lipid parameters, and total ghrelin levels were evaluated at baseline and following 12 weeks of treatment. Results: Significant decreases in body weight and body mass index were observed over the entire study period in both treatment groups. Glycosylated hemoglobin and postprandial plasma glucose levels were statistically significant decreased in the groups receiving sitagliptin compared with baseline values (p = 0.021 and p = 0.021, respectively), while they were unchanged in the groups receiving MNT. There was a significant decrease in total ghrelin in the groups receiving sitagliptin (p = 0.04) compared with baseline values but not in the groups receiving MNT (p = 0.46) at the end of the 12 weeks. Conclusions: In this study of patients with type 2 diabetes, treatment with sitagliptin was associated with a significant decrease in serum ghrelin levels. These results suggest that the neutral effect of sitagliptin on weight might be associated with the suppression of fasting serum ghrelin levels.Item Effects of disease severity and necrosis on pancreatic dysfunction after acute pancreatitis(Baishideng Publishing Group, 2013-11-28) Garip, Gökhan; Sarandöl, Emre; Kaya, Ekrem; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Cerrahi Anabilim Dalı.; 0000-0002-2593-7196; 0000-0002-9562-4195; ABE-1716-2020; AAG-7319-2021; 40261542500; 55943324800; 7004568109AIM: To evaluate the effects of disease severity and necrosis on organ dysfunctions in acute pancreatitis (AP). METHODS: One hundred and nine patients treated as AP between March 2003 and September 2007 with at least 6 mo follow-up were included. Patients were classified according to severity of the disease, necrosis ratio and localization. Subjective clinical evaluation and fecal pancreatic elastase-I (FPE-I) were used for exocrine dysfunction evaluation, and oral glucose tolerance test was completed for endocrine dysfunction. The correlation of disease severity, necrosis ratio and localization with exocrine and endocrine dysfunction were investigated. RESULTS: There were 58 male and 51 female patients, and mean age was 56.5 +/- 15.7. Of the patients, 35.8% had severe AP (SAP) and 27.5% had pancreatic necrosis. Exocrine dysfunction was identified in 13.7% of the patients [17.9% were in SAP, 11.4% were in mild AP (MAP)] and 34.7% of all of the patients had endocrine dysfunction (56.4% in SAP and 23.2% in MAP). In patients with SAP and necrotizing AP (NAP), FPE-I levels were lower than the others (P < 0.05 and 0.001 respectively) and in patients having pancreatic head necrosis or near total necrosis, FPE-1 levels were lower than 200 mu g/g stool. Forty percent of the patients who had undergone necrosectomy developed exocrine dysfunction. Endocrine dysfunction was more significant in patients with SAP and NAP (P < 0.001). All of the patients in the necrosectomy group had endocrine dysfunction. CONCLUSION: Patients with SAP, NAP, pancreatic head necrosis and necrosectomy should be followed for pancreatic functions.Item Effects of green tea on serum paraoxonase/arylesterase activities in streptozotocin-induced diabetic rats(Pergamon-Elsevier Science, 2005-12) Taş, Sibel; Sarandöl, Emre; Ziyanok, Sedef; Aslan, Kemal; Dirican, Melahat; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; AAH-4272-2021; ABE-1716-2020; AAG-6985-2021; ABE-6873-2020In recent years, green tea has become a subject of interest because of its beneficial effects on human health. The purpose of this study was to determine the effects of green tea on serum paraoxonase/arylesterase activities and lipoprotein oxidizability in streptozotocin-induced diabetic rats (65 mg/kg [intraperitoneal]). Green tea was given in tap water (2%) for 3 and 6 weeks to control (CGT-3w and CGT-6w) and diabetic (DGT-3w and DGT-6w) rats, and they were compared with the control and diabetic groups (D-3w and D-6w), respectively. Serum insulin level was significantly increased in the DGT-6w group; serum lipid and plasma and tissue malondialdehyde levels were reduced in the DGT-3w and DGT-6w groups. Oxidizability of apolipoprotein B-containing lipoprotein fraction was found to be significantly reduced in the DGT-6w group. Serum total antioxidant capacity showed a significant increase in the CGT-6w and DGT-6w groups. Paraoxonase activity was significantly reduced in the D-3w and D-6w groups and increased in the DGT-6w group. We conclude that green tea might have antilryperlipidernic and antioxidative effects and may slow the progression of atherogenesis by reducing oxidation of lipoproteins and preserving paraoxonase activity.Item Effects of hypoxic preconditioning in antioxidant enzyme activities in hypoxic-ischemic brain damage in immature rats(Türk Nöroşurji Derneği, 2008-04) Alkan, Tülin; Gören, Bülent; Vatansever, Ebru; Sarandöl, Emre; Uludağ Üniversitesi/Tıp Fakültesi/Fizyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; 0000-0001-6466-5042; 0000-0002-2593-7196; AAH-1792-2021; ABE-1716-2020; AAH-1718-2021; 6601953747; 6602543716; 16070622700; 55943324800Aim: HI (hypoxic-ischemic) brain injury is a major cause of neonatal mortality and longterm neurological morbidity. The aim of the present study was to investigate the effects of HPC (hypoxic preconditioning) on the oxidative-antioxidative status in the neonatal HI brain model. Material and Methods: Fifty five 7-day-old rats were placed into; Control, HPC, HPC+HI insult, and HI insult groups. HPC, The HPC+HI insult groups were subjected to hypoxia (37°C, 8%O2) and the control group to normoxia for 2.5 hrs. Twenty-four hours later, the rats in the HPC+HI insult and HI insult groups were exposed to cerebral HI produced by unilateral right common carotid artery (CCA) occlusion combined with 90 min hypoxia. Four hours after recovery, the malondialdehyde (MDA) level and the activities of superoxide dismutase (SOD), and glutathione peroxidase (GPx) were determined in the brain tissues of the rats. Results: The findings of the present study suggest increased lipid peroxidation and/or decreased antioxidant activity in the brain of the HI rats. Conclusion: The beneficial effects of HPC might not be related to the alterations in the antioxidative activity.Item Effects of long-term antidepressant treatment on oxidative status in major depressive disorder: A 24-week follow-up study(Pergamon-Elsevier Science, 2011-07-19) Kotan, Vahap Ozan; Kırhan, Emine; Özkaya, Güven; Kirli, Selçuk; Sarandöl, Emre; Uludağ Üniversitesi/ Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/ Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; Uludağ Üniversitesi/ Tıp Fakültesi/Psikiyatri Anabilim Dalı.; 0000-0002-2593-7196; 0000-0003-0297-846X; ABE-1716-2020; A-4421-2016; 37104411100; 16316866500; 14019745700; 55943324800Purpose: Major depressive disorder (MDD) is a devastating disease that afflicts large populations and has also been accepted to be an independent risk factor for cardiovascular disease (CVD). Oxidative stress seems to play an essential role in the relationship of MOD and CVD. We aimed to determine the level of oxidative stress in patients with MOD and to investigate the effects of long-term antidepressant (AD) treatment on the oxidative-antioxidative system parameters and CVD risk factors. Method: Fifty patients who fully met the fourth Diagnostic and Statistical Manual of Mental Disorders criteria for MOD and 44 healthy control subjects were included in the study. Control visits of the patients were repeated 6 weeks, 12 weeks and 24 weeks after beginning of the AD treatment Lipid profiles, oxidation and oxidizability of apolipoprotein B-containing lipoproteins (expressed as apo B-b-MDA and apo B-Delta-MDA, respectively), levels of plasma malondialdehyde (p-MDA), total antioxidative capacity (TAOC), antioxidant molecules and antioxidant enzyme activities including paraoxonase/arylesterase, red blood cell superoxide dismutase (RBC-SOD) and glutathione peroxidase were determined during 24-week of follow-up period. Results: According to the results of the study, p-MDA, apo B-b-MDA and RBC-SOD activity were increased and arylesterase activity was decreased in MDD patients. Body mass index (BMI), vitamin A and total cholesterol levels in MDD patients increased after 24-weeks of AD treatment RBC-SOD activity, TAOC, p-MDA and apo B-b-MDA levels were decreased: paraoxonase/arylesterase activities and apo B-Delta-MDA were increased at the end of 24th week. Conclusion: Oxidative stress, demonstrated in MDD patients, was partly improved during 24 weeks of AD treatment Increase in paraoxonase/arylesterase activities and decrease in p-MDA and apo B-b-MDA levels after 24 weeks seem to be beneficial for reduction of CVD risk in MDD patients. However increased BMI and apo B-Delta-MDA levels are negative cardiovascular effects of long-term AD treatment.Item Effects of long-term consumption of high fructose corn syrup containing peach nectar on body weight gain in sprague dawley rats(Soc Brasileira Ciencia Tecnologia Alimentos, 2016-10-23) Sinir, Gülşah Özcan; Suna, Senem; İnan, Sevda; Bağdaş, Deniz; Tamer, Canan Ece; Çopur, Ömer Utku; Sığırlı, Deniz; Sarandöl, Emre; Sönmez, Gürsel; Ercan, İlker; Evrensel, Türkkan; Tarım, Ömer Faruk; Eren, Erdal; Uylaser, Vildan; İncedayı, Bige; Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği Bölümü.; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Deney Hayvanları Yetiştirme ve Araştırma Merkezi.; Uludağ Üniversitesi/Tıp Fakültesi/Biyoistatistik Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Onkoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Endokrinolojisi Anabilim Dalı.; 0000-0003-3954-0058; 0000-0002-1684-1053; 0000-0001-8138-5851; 0000-0002-9732-5340; 0000-0002-2593-7196; 0000-0002-6947-2167; AAF-3324-2020; AAH-1155-2021; AAG-8336-2021; AAM-1734-2020; E-3364-2018; AAG-8503-2021; AAG-8277-2021; AAG-8241-2021; AAJ-1027-2021; ABE-1716-2020; AAG-8410-2021; AAQ-8178-2020; AAP-7233-2020; ABF-2367-2020; AAA-7472-2021; 57194441710; 55512747500; 56320836200; 15062425700; 8228159500; 8228159600; 24482063400; 55943324800; 55167435000; 6603789069; 6603942124; 6701427186High fructose corn syrup (HFCS) is one of the most used sweeteners in the food industry. Health concerns regarding the consumption of HFCS-containing foods have developed in parallel with the increasing amount of people who become overweight. This study was conducted to investigate whether HFCS-containing peach nectar (pn-HFCS) consumption has more detrimental effects on anthropometrical and biochemical parameters compared with sucrose-containing peach nectar (pn-sucrose). Fifty-day-old Sprague Dawley rats were divided into three groups and were fed (A) pn-HFCS + ad libitum chow, (B) pn-sucrose + ad libitum chow and (C) only ad libitum chow for 7 months. The percentage change in body weight (PCBW), body mass index (BMI), and Lee index were calculated, and serum triglyceride, glucose, insulin and leptin concentrations were measured. The PCBW, BMI, Lee index, serum triglyceride, glucose, insulin and leptin concentrations were insignificant among the three groups. We can suggest that peach nectar consumption resulted in more energy intake than the control and since pn-HFCS group consumed more chow than the pn-sucrose group. The results show that long term daily HFCS or sucrose consumption in peach nectar is not associated with weight gain and does not stimulate metabolic changes in Sprague Dawley rats.Item Effects of red wine consumption on serum paraoxonase/arylesterase activities and on lipoprotein oxidizability in healthy-men(Elsevier, 2003-09) Sarandöl, Emre; Serdar, Zehra; Dirican, Melahat; Şafak, Özlem; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; 0000-0002-2593-7196; ABE-1716-2020; 55943324800; 57222002284; 6601919847; 6508363078Although there is a general consensus concerning the lower risk for cardiovascular disease in moderate drinkers, the mechanisms responsible for the cardioprotective effect of red wine remain unknown. It has been proposed that increased serum paraoxonase activity may be a mechanism of action underlying reduced cardiovascular disease risk in moderate drinkers, since paraoxonase inhibits lipoprotein oxidation. The aim of this study was to investigate the effects of red wine consumption on serum paraoxonase/arylesterase activities and on lipoprotein oxidizability in healthy-men. Fourteen healthy-men were included in the study. The subjects consumed 0.375 g alcohol / kg body weight for 3 weeks. Paraoxonase and arylesterase activities were studied spectrophotometrically. Oxidizability of apolipoprotein B-containing lipoproteins were determined, after separating them with precipitation method, by incubating with copper-sulfate. Paraoxonase activity did not change, however arylesterase activity significantly decreased after red wine consumption (P < 0.01). There was a reduced susceptibility of apolipoprotein B-containing lipoproteins to copper-sulfate induced oxidation after red wine consumption (P < 0.01). Our results support that red wine protects lipoproteins against oxidation, however there was not any significant change in serum paraoxonase activity after red wine consumption.Item Effects of thiamine treatment on oxidative stress in experimental diabetes(Aepress Sro, 2020) Sarandöl, Emre; Taş, Sibel; Serdar, Zehra; Dirican, Melahat; Uludağ Üniversitesi/Tıp Fakültesi Fakültesi/Temel Tıp Bilimleri Bölümü.; Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; ABE-1716-2020; ABE-6873-2020; AAH-6200-2021; ESK-6562-2022; 0000-0002-2593-7196; 0000-0002-0909-618X; 55943324800; 7004343411; 57222002284; 6601919847AIM: Hyperglycemia, oxidative stress and hyperlipidemia are features of diabetes mellitus. Thiamine has beneficial effects on carbohydrate metabolism and it was proposed that this vitamin has antihyperlipidemic and antioxidant effects. Our aim was to investigate the effects of thiamine on oxidative stress and metabolic changes in streptozotocin (STZ) induced diabetic rats. METHOD: Diabetes was induced by a single intraperitoneal injection of STZ. Thiamine (6 mg/kg) was added to drinking water for five weeks. The rats were divided into four groups: control rats; thiamine treated control rats; diabetic rats; thiamine treated diabetic rats. Plasma and tissue malondialdehyde (MDA) levels were measured by high-performance liquid chromatography and spectrophotometry, respectively. Paraoxonase (PON) and arylesterase (AE) activities were measured with spectrophotometric methods, and erythrocyte superoxide dismutase (SOD) and blood glutathione peroxidase (GSH-Px) activities were determined using commercial kits. RESULTS: Thiamine treatment reduced plasma and tissue MDA levels, serum glucose, total cholesterol and triglyceride levels, and increased serum high density lipoprotein-cholesterol and insulin levels, serum PON and AE, erythrocyte SOD and blood GSH-Px activities. CONCLUSION: Thiamine significantly improves oxidative stress and has hyperinsulinemic and antihyperlipidemic effects so we suggest that thiamine might be used as a supportive therapeutic agent in diabetes (Tab. 2, Fig. 3, Ref. 53).
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