Browsing by Author "Seyrek, Kamil"
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Item Apoptosis rate in malignant mammary tumours in bitches(Ecole Nationale Veterinaire Toulouse, 2011-03) Seyrek, Kamil; Köse, Hayrullah; Akşit, Hasan Z.; Bıldık, Ayşegül; Sandıkçı, Mustafa; İntaş, Kamil Seyrek; Özalp, Gözde Rabia; Mecitoğlu, Gülnaz Yılmazbaş; Özyiǧit, Musa Özgür; Uludağ Üniversitesi/Veterinerlik Fakültesi/Doğum, Jinekoloji ve Üreme Anabilim Dalı.; Uludağ Üniversitesi/Veterinerlik Fakültesi/Patoloji Anabilim Dalı.; 0000-0003-2817-3221; AAH-7292-2019; AAG-4440-2019; AAR-6478-2021; 6603409870; 23985710500; 36457964000; 6507338060As the equilibrium between cell division and cell death (apoptosis) determines the growth of a tumour, the aim of the study was to evaluate the apoptosis frequency in dog mammary tumours both by the TdT-mediated dUTP-biotin nick end Libelling (TUNEL) method and by the ELISA detection of nucleosoms in cell homogenates. For that, apoptosis rates were investigated in 35 malignant tumours (16 malignant mixed tumours, 12 lobular adenocarcinomas, 3 cirrhotic adenocarcinomas and 4 myoepitheliomas) and in 10 normal mammary tissues. Whereas the apoptotic cells were found abundant and homogeneously distributed throughout the healthy breast tissues by the TUNEL method, they appeared less numerous in malignant tumours and were essentially located within mammary acini in few areas. The average amounts of nucleosoms proportional to the absorbance at 405 nm in the ELISA test in cell homogenates from malignant tumours were lower than in the healthy mammary tissues but according to the tumour type, the difference with controls was significant only for the malignant mixed tumours (P < 0.05). These results show that apoptosis rate tended globally to decrease in dog malignant mammary tumours, favouring in this way the selection and expansion of apoptosis-resistant neoplastic cells.Item Biochemical and histochemical detection of the sialic acids in mammary tumours of bitches(Ecole Nationale Veterinaire Toulouse, 2005-05) Seyrek, Kamil; Kıral, Funda Kargın; Musal, Bayazıt; Toplu, Nihat; Seyrek, Kamil İntaş; Keskin, Abdulkadir; Uludaǧ Üniversitesi/Veterinerlik Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.; AAH-7292-2019; 6603409870; 24473229800This study describes the expression and localisation of sialic acid in mammary tumours in bitches. Tissue total sialic acid (TSA) concentrations were detected spectrophotometrically. Localisation of sialic acids in mammary tissue was visualised using the biotin-conjugated Sambucus nigra lectin (SNA), specific for alpha 2,6-linked sialic acid, and Maaickia amurensis lectin (MAL), specific for alpha 2,3-linked sialic acid. The mean value of tissue TSA of malignant tumours (1.46 +/- 0.14 mu mol/g protein) was significantly higher (p < 0,001) than that in healthy tissues (0.26 +/- 0.07 mu mol/g protein) as well as in tissues with benign types of tumour (0.50 +/- 0.06 mu mol/g protein). We could not find a significant difference in sialic acid concentrations between adenomas and normal tissue (p > 0.05). Tissue TSA content in the same type of tumours was not always equal. In histochemical analyses both the staining intensity and localisation patterns of SNA and MAL showed marked differences. The staining for SNA and MAL was only low or not present in normal mammary tissues but high in carcinomas. In malign tumours binding sites for SNA were predominantly in epithelial cells and in individual cells infiltrating the fibrous tissue. However, the binding sites for MAL were predominantly in fibrous tissue, capillary and lymphatic endothelia. Contrary to malignant tumours, a moderate staining was observed in benign tumours. The staining was mainly in epithelial cells, in fibrocytes and in myoepithelial cells. It was not always the case that the same type of tumours revealed the same staining intensity localisation for the lectins. The results obtained from the present study suggest that elevated levels of sialic acid in malignant mammary tumours may play an important role in the detachment of carcinoma cells from the primary tumour and would be of critical importance for the metastasis of turnout cells.Item The effects of vincristine sulfate on expression of galectin-3, Bcl-2, and carbohydrate structures specific for EEL, GSL-1, and RCA-1 lectins in bitches with naturally occurring canine transmissible venereal tumor(Scientific and Technological Research Council Turkey, 2014-02-03) Akşıt, Hasan Z.; Seyrek, Kamil; Özyiğit, Musa Özgür; Nak, Deniz; İnan, Sevda; Şimşek, Gözde; Nak, Yavuz; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Kadın Hastalıkları ve Doğum Anabilim Dalı.; Uludağ Üniversitesi/Veteriner Fakültesi/Biyoistatistik Anabilim Dalı.; 0000-0001-8138-5851; 0000-0002-9634-0055; AAH-2873-2021; AAH-5494-2021; E-3364-2018; 6507338060; 9280090000; 56320836200; 26435497200; 8615464000Vincristine sulfate is one of the most effective chemical agents used in canine transmissible venereal tumor (CTVT) chemotherapy. Its therapeutic effectiveness is pronounced and significant at the beginning of treatment. The present study was designed to investigate vincristine sulfate treatment and the relationship between CTVT tumor regression and apoptosis [estimated by TdT-mediated dUTP-biotin nick end labeling (TUNEL)], galectin-3 (H-160), Bcl-2 (N-9), and lectin [EEL (B-135), GSL I (L-1100), and RCA I (BA-0084)] expression using immunohistochemical staining in 20 bitches with naturally occurring CTVTs. Evaluation was semiquantitatively performed by HSCORE values using light microscopy All observed staining was intracytoplasmic and increased endothelial staining was noted with the Bcl-2 marker in tumor vessels. Apoptosis, TUNEL, and lectin scoring was higher in vincristine sulfate-treated tumors than in untreated tumors, while expression of antiapoptotic factors galectin-3 and Bcl-2 were lower in treated than in untreated CTVTs. These results show that galectin-3, Bcl-2, and lectins (EEL, GSL-1, and RCA-1) are suitable indicators/markers for CTVT tumor regression after treatment with vincristine sulfate.Item Levels of zinc, copper and magnesium in sheep with toxoplasmosis(Uludağ Üniversitesi, 2004-05-31) Seyrek, Kamil; Paşa, Serdar; Kıral, Funda; Bildik, Ayşegül; Babür, Cahit; Kılıç, SelçukThe aim of the present study was to investigate the effects of Toxoplasma gondii infection on the content of essential elements of zinc (Zn), copper (Cu), and magnesium (Mg) in sheep. Serum Zn, Cu, and Mg levels as well as wool Zn and Cu concentrations were measured in twenty sheep whose anti-T gondii antibodies were positive and in seventeen sero-negative healthy sheep. The average Zn concentration in serums from sero-positive sheep was significantly (P<0.05) lower than in their controls. However, compared to the healthy ewes the Cu concentrations in serum of sero-positive objects were recorded significantly (P<0.001) higher. The mean concentration of Mg in serum was higher but not significant (P>0.05) in sero-positive sheep than in their controls. On the other hand, the Zn and Cu concentrations of wool from diseased animals showed no significant elevations.Item Protective effect of 2-aminoethyl diphenylborinate on acute ischemia-reperfusion injury in the rat kidney(Elsevier, 2014-04) Yıldar, Murat; Akşit, Hasan Z.; Korkut, Oǧuzhan; Sunay, Bahar; Seyrek, Kamil; Özyiǧit, Musa Özgür; Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; AAR-6478-2021; 6507338060Background: To investigate the protective effect of 2-aminoethyl diphenylborinate (2-APB) against ischemia-reperfusion (I/R) injury in the rat kidney by an experimental study. Materials and methods: Thirty male Sprague-Dawley rats were randomly divided into the following three groups: (1) sham group, (2) I/R group, and (3) I/R + 2-APB group. Renal I/ R injury was induced by clamping the left renal pedicle for 45 min after right nephrectomy, followed by 3 h of reperfusion. The therapeutic agent 2-APB was administered intravenously at a dose of 2 mg/kg 10 min before renal ischemia. Glutathione, superoxide dismutase, total antioxidant capacity, malondialdehyde, tumor necrosis factor a, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels were measured from blood samples, and the rats were sacrificed subsequently. Tissue samples were scored histopathologically. Visualization of apoptotic cells was performed using the terminal deoxynucleotidyl transferase dUTP nick end labeling staining method. Results: 2-APB significantly reduced serum malondialdehyde, tumor necrosis factor a, interleukin 6, aspartate aminotransferase, alanine aminotransferase, and creatinine levels in the I/R injury group. However, glutathione, superoxide dismutase, and total antioxidant capacity levels increased significantly. Histopathologic scores were significantly better and the rate of apoptosis was lower in the 2-APB group. Conclusions: 2-APB reduces oxidative stress and damage caused by renal I/R injury. The results of this study demonstrate that 2-APB can be used as an effective agent against I/R injury in the kidney.Item Serum sialic acid, total protein and albumin levels in cows with metritis treated with intrauterine oxytetracycline(Uludağ Üniversitesi, 2004-11-03) Musal, Bayazıt; Seyrek, Kamil; Ulutaş, Pınar A.Serum total sialic acid, total protein and albumin concentrations in healthy cows and cows with metritis treated with local intrauterine therapy were measured. Twenty multiparous cows with metritis from a dairy herd were treated with a daily dose of 25 ml intrauterine injectable oxytetracycline solution (100 mg/ml) for three consecutive days. A significant reduction (p<0.001) of vaginal discharge following therapy were observed while this reduction could not be corraleted with mean serum sialic acid concentrations which were measured as 896 µg/ml in healthy cows and 1063,19 µg/ml before and 1053,40 µg/ml following the treatment. No difference were observed in serum total protein and albumin concentrations of healthy and sick cows.Item Sığır pankreaslarındaki galaktoz rezidülerinin mistellektin-1 ile saptanması(Uludağ Üniversitesi, 2000-11-22) Seyrek, Kamil; Kaltner, HerbertFötal gelişimin normal seyri için farklı gruptan moleküller arasında şekillenen etkileşimler esansiyeldir. Özellikle glikokonjugatların (glikoproteinler, glikolipitler, proteoglikanlar vs.) yapısında bulunan karbonhidratlar ünitelerinin bazı spesifik proteinlerle interaksiyonları büyük önem taşımaktadır. Proteinlerin yapıtaşlarını oluşturan amino asitlerin yanı sıra karbonhidratların da yapılarında biyolojik bilgi taşıdıkları ve bunları önemli birçok fizyolojik olayın şekillenmesinde kullandıkları düşünülmektedir. Bu karbonhidrat rezidüleri spesifik proteinler (lektinler) tarafından tanınıp bağlanmakta ve içerdikleri biyolojik kod yine bu proteinler tarafından deşifre edilmektedir. Bitkisel lektinler hücreler ve dokulardaki karbonhidrat strüktürlerinin varlığının ve lokalizasyonunun araştırılmasında son zamanlarda sıkça kullanım alanı bulmaktadırlar. Biz bu çalışmada β-D-galaktoza spesifik bir lektin olan mistellektin I’in pankreastaki ligantlarının tespitine çalıştık. Bunun için biotin ile işaretlenmiş mistellektin I kullanıldı. Sonuç olarak, fötal gelişimin seyri sırasında reaksiyonların görüldüğü hücre çeşitlerinde ve reaksiyon yoğunluğunda devamlı bir değişkenliğin bulunduğu gözlendi.