Browsing by Author "Simon, Philipp"

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Genetic characterization of allium tuncelianum: An endemic edible allium species with garlic odor
(Elsevier, 2008-02-21) Simon, Philipp; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 16031208900; 6603912487
Allium tuncelianum (Kollman) Ozhatay, Matthew & Siraneci is a native species to the Eastern Anatolia. Its plant architecture resembles garlic (Allium sativum L.) and it has mild garlic odor and flavor. Because of these similarities between two species, A. tuncelianum has been locally called "garlic". In addition, both A. tuncelianum and garlic has 16 chromosomes in their diploid genomes. Recently, A. tuncelianum has been suggested as the wild progenitor species of garlic. In this study, amplified fragment length polymorphisms (AFLP) markers and nucleotide sequence analysis of the internal transcribed spacer region (ITS) were used to assess genetic and phylogenetic relationships among A. tuncelianum, garlic and some other Allium species. AFLP analysis demonstrated that A. tuncelianum and garlic are genetically distinct and they are likely different species. Phylogenetic analyses based on the nucleotide sequence of ITS suggested that A. tuncelianum and garlic are distinct species and placed A. tuncelianum, garlic, Allium ampeloprasum and Allium scorodoprasum into the same clade in the neighbor joining dendrogram and in the consensus tree of parsimony analysis. However, A. tuncelianum was phylogenetically less related to garlic than either A. ampeloprasum or A. scorodoprasum, suggesting that A. tuncelianum may not be the immediate wild ancestor species of garlic. Further studies to generate hybrid progeny between A. tuncelianum and garlic (if possible) could provide more information on the homology between the chromosomes of A. tuncelianum and garlic and genetic relationships between these two species.
Genetic diversity assessment of garlic clones with SSR markers
(Amerikan Society Horticultural Science, 2016-09) Simon, Philipp; İpek, Ahmet; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi.; AAK-4655-2021; AAH-3233-2021
Genetic diversity assessment of garlic clones with SSR markers
(Amer Soc, 2016-09) Simon, Philipp; İpek, Ahmet; İpek, Meryem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; AAK-4655-2021
Molecular characterization of Kastamonu garlic: An economically important garlic clone in Turkey
(Elsevier, 2008-01-07) Simon, Philipp; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 6603912487
Turkey is one of the major garlic producing country in the World and the significant amount of Turkey's production has been made using a garlic variety called Kastamonu garlic. Therefore, the purpose of this study was to assess genetic relationship of Kastamonu garlic with 20 previously characterized garlic clones collected from different regions of the world using AFLP and locus specific DNA markers. One putative Kastamonu garlic genotype was obtained from Taskopru district of Kastamonu province while another putative Kastamonu garlic genotype was collected from a local farmers' market in Bursa province and called as Kast-Taskopru and Kast-Bursa in this study, respectively. In the UPGMA dendrogram developed by using 120 AFLP markers, Kast-Taskopru was clustered closely over 97% similarity with other non-bolting garlic clones, P1493112, P1493118 and P1383824. This cluster was also supported by bootstrap analysis with 100% bootstrap value. All clones in this cluster also shared same alleles of gene specific DNA markers. However, Kast-Bursa shared 100% polymorphic AFLP markers and gene specific markers with a different garlic clone, P1497951 in another distinct cluster of UPGMA dendrogram and this clustering has also bootstrap value of 100%. These results suggest that Kastamonu garlic is not unique and garlic production in Turkey has been made using several garlic clones, even though most of this production has been sold as Kastamonu garlic due to its high popularity. Therefore, a standard Kastamonu garlic genotype needs to be determined by fingerprinting all available garlic clones cultivated in Kastamonu province and other regions of Turkey.
Rapid characterization of garlic clones with locus-specific DNA markers
(Tubitak Scientific & Technical Research Council Turkey, 2008) Simon, Philipp; İpek, Meryem; İpek, Ahmet; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; AAH-3233-2021; 16031208900; 6603912487
Maintenance of redundant garlic (Allium sativum L.) accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. For this purpose we developed several locus-specific polymerase chain reaction (PCR)-based DNA markers, and tested them for the characterization of garlic clones that were previously analyzed using amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) markers. Among 25 garlic clones, 4 locus-specific markers generated 24 polymorphic alleles. Garlic accessions within a group determined by AFLP and RAPD analyses generally had the same alleles as the locus-specific markers. In addition. several alleles of these locus-specific markers were associated only with certain AFLP and RAPID groups, which can allow rapid assessment of newly collected garlic accessions; therefore. these locus-specific markers can be used as another tool for the rapid characterization of garlic germplasm collections.