Browsing by Author "Takanlou, Leila Sabour"
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Item Clinicopathologic features and genetic characteristics of the BRCA1/2 mutation in Turkish breast cancer patients(Elsevier Science, 2019-10-14) Eskiler, Gamze Güney; Çeçener, Gülşah; Takanlou, Leila Sabour; Takanlou, Maryam Sabour; Egeli, Ünal; Aksoy, Seçil; Ünal, Ufuk; Tezcan, Havva; Eryılmaz, Işıl Ezgi; Gökgöz, Mustafa Şehsuvar; Tunca, Berrin; Çubukçu, Erdem; Evrensel, Türkkan; Çetintaş, Sibel; Taşdelen, İsmet; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji ve Genetik Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Onkoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Radyasyon Onkolojisi Anabilim Dalı.; 0000-0002-3820-424X; 0000-0001-7904-883X; 0000-0002-3760-9755; 0000-0003-4913-3616; 0000-0002-3316-316X; 0000-0002-1619-6680; 0000-0002-9732-5340; GGI-6227-2022; EAS-6830-2022; GYU-0252-2022; EWY-5692-2022; ETP-1691-2022; EOI-5652-2022; EBN-1186-2022; 6508156530; 57211585974; 57211582304; 55665145000; 57193933334; 57211584917; 57211580953; 57189380840; 57203870909; 6602965754; 53986153800; 6603942124; 6505881756; 9637821500The aim of this study was to identify the frequency and spectrum of germline BRCA1/2 pathogenic alterations in a cohort of patients with breast carcinoma. In this study, a total of 603 breast cancer subjects from Turkey were screened for BRCA1/BRCA2 mutations using HDA and Sanger sequencing. In the present study, 21 BRCA1 and BRCA2 pathogenic variants were detected in 30 patients and BRCA1/2 mutations were significantly associated with a family history of breast/ovarian cancer. Analysis of overall survival for BRCA1/BRCA2 mutation carriers showed a trend for poor overall survival only in BRCA1 carriers, although this was not statistically significant in BRCA1 and BRCA2 mutation carriers. The c.5266dupC mutation is one of the most frequently reported mutations in BRCA1 and was identified in five breast cancer patients in our study. The most common BRCA2 gene mutations in the present study were c.8940delA and c.9097dupA, which were found in seven patients. We found mostly BRCA1 and BRCA2 mutation carriers in those patients who showed hormone-positive features. In conclusion, our data showed differences in the distribution of the mutation spectrum of BRCA1 and BRCA2 in Turkey.Publication Correlation between ubiquitin e3 ligases (siahs) and heat shock protein 90 in breast cancer patients(Iranian Scientific Society Medical Entomology, 2022-08-01) Takanlou, Leila Sabour; Çeçener, Gülşah; Takanlou, Maryam Sabour; Nazlioglu, Hulya Ozturk; Unlu, Havva Tezcan; Isik, Ozgen; Egeli, Unal; Tunca, Berrin; Çubukcu, Erdem; Tolunay, Sahsine; Gokgoz, Mustafa Sehsuvar; Cecener, Gulsah; ÇEÇENER, GÜLŞAH; Nazlioglu, Hulya Ozturk; ÖZTÜRK NAZLIOĞLU, HÜLYA; Isik, Ozgen; IŞIK, ÖZGEN; Egeli, Unal; EGELİ, ÜNAL; Tunca, Berrin; TUNCA, BERRİN; Çubukcu, Erdem; ÇUBUKÇU, ERDEM; Tolunay, Sahsine; TOLUNAY, ŞAHSİNE; 0000-0002-1928-992X; 0000-0002-3820-424X; 0000-0002-1590-4833; 0000-0002-0910-4258; 0000-0002-9541-5035; 0000-0001-7904-883X; 0000-0002-1619-6680; KGL-6846-2024; AAH-1420-2021; GRE-6268-2022; AAW-9602-2020; GYU-0252-2022Background: Breast cancer is a heterogeneous disease and differences in the expression levels of the ER, PR, and HER2 the triplet of established biomarkers used for clinical decision-making have been reported among breast cancer patients. Furthermore, resistance to anti-estrogen and anti-HER2 therapies emerges in a considerable rate of breast cancer patients, and novel drug therapies are required. Several anomalous signaling pathways have been known in breast cancer have been known; heat shock protein 90 (HSP90) is one of the most plenty proteins in breast cells. The family of ubiquitin ligases such as SIAH1 and SIAH2 is known to specifically target misfolded proteins to the proteasome; also, they have been illustrated to play a role in RAS signaling and as an essential downstream signaling component required for EGFR/HER2 in breast cancer.Methods: The expression of SIAH2, HSP90, and HER2 was assessed by quantitative Real-Time PCR in 85 invasive ductal carcinoma breast tumor samples at Uludag University Hospital in Turkey during the years 2018-2019, and its association with the clinicopathologic variables of patients was evaluated.Results: HSP90, SIAH1, and SIAH2 were significantly (P=0.0271, P=0.022, and P=0.0311) upregulated tumor tissue of patients with breast cancer. Moreover, this study observed a significant association between the high expression of SIAH2/ HSP90 with ER status, high expression of HSP90 with Recurrence/ Metastasis, and high expression of SIAH2 with Ki-67 proliferation index.Conclusion: The HSP90 and SIAH2 expressions play a significant role in breast cancer development by combining the experimental and clinical data obtained from the literature.Item Retrospective analysis of her2 / hsp90 / sıah gene expressions in ınvasive ductal carcinoma breast cancer patients(Bursa Uludağ Üniversitesi, 2019-08-19) Takanlou, Leila Sabour; Çeçener, Gülşah; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü/Tıbbi Biyoloji Anabilim Dalı.Meme kanseri kadınlar arasında en sık görülen kanser türüdür. Sıklığı sürekli artış göstermekte olup, önde gelen ölüm nedeni olmaya devam etmektedir. İnsan epidermal büyüme faktör reseptörü 2 (HER2), tirozin kinaz aktivitesine sahip epidermal büyüme faktör reseptör ailesinin bir üyesidir ve meme kanserinin önemli prognostik faktörlerinden biridir. HER2 için hedefe yönelik tedavilerin başlamasıyla HER2 pozitif kanserli hastaların klinik seyrinde ciddi iyileşme sağlanmıştır. Günümüzde HER2 testi değişik yöntemlerle yapılmaktadır ve HER2 ifade durumunu doğru değerlendirebilmek için bu testlerin standardizasyonu çok önemlidir. Ayrıca HER2 geni, ısı şok protein ailesinin bir üyesi olan HSP90’ı hedefleyerek aktivitesini arttırmaktadır. SIAH (yedi homolog homolog-2) gibi E3 ubiquitin ligazlar ise, HSP90’a bağlanarak hedef proteinlerin parçalanmasına yol açan ko-şaperon olarak görev yaparlar. Bu çalışmada, RT-PCR yöntemi ile İHK yöntemi arasındaki korelasyonun değerlendirilmesi amaçlanmıştır. İlaveten, invaziv duktal meme kanserinde HER2 / HSP90 / SIAH1 / SIAH2 genlerinin ifadeleri ve ifade farklılıklarının hastaların klinikopatolojik özelikleri ile karşılaştırılarak prediktif ve prognostik önemlerinin değerlendirilmesi amaçlanmıştır. Mevcut çalışmada 94 invaziv meme kanserli hastada HER-2 ifade durumu değerlendirilerek elde edilen bulgular ile HER2 mRNA ifadelerinin İHK ile 74.35% oranında korele olduğu belirlendi. HER2-pozitif meme kanserli hastaların tümör dokularında HSP90 ve SIAH2 genlerinin ifadelerinin istatistiksel olarak anlamlı olarak up-regüle edildiği belirlendi (p= 0.047; p= 0.024). İnvaziv duktal meme kanserli hastalarda HSP90 ve HER2 mRNA ekspresyonunun arasında korelasyon olduğu, ayrıca SIAH2 mRNA ekspresyonunun, HSP90 ve HER2 ekspresyonları ile korelasyon gösterdiği belirlendiPublication The expression and prognostic value of miR-146a and miR-155 in Turkish patients with multiple sclerosis(Taylor & Francis, 2022-03-04) Sarıdaş, Furkan; Ünlü, Havva Tezcan; Çeçener, Gülşah; Egeli, Ünal; Takanlou, Maryam Sabour; Takanlou, Leila Sabour; Tunca, Berrin; Zarifoğlu, Mehmet; Turan, Ömer Faruk; Taşkapılıoğlu, Özlem; SARIDAŞ, FURKAN; ÇEÇENER, GÜLŞAH; EGELİ, ÜNAL; TUNCA, BERRİN; ZARİFOĞLU, MEHMET; TURAN, ÖMER FARUK; Takanlou, Maryam Sabour; Takanlou, Leila Sabour; Taşkapılıoğlu, Özlem; 0000-0001-5945-2317; 0000-0002-0910-4258; 0000-0002-3820-424X; 0000-0001-7904-883X; 0000-0002-1590-4833; 0000-0002-6361-7150; 0000-0002-1619-6680; HSB-2700-2023; GYU-0252-2022; AAP-9988-2020; AAH-1420-2021; KGL-6846-2024; GRE-6268-2022; ABI-6078-2020; EHN-5825-2022; JDI-6091-2023; EBA-4926-2022Multiple sclerosis (MS) is an inflammatory, autoimmune demyelinating, and neurodegenerative disorder of the central nervous system. Interactions between environmental factors, predisposition genes, and determining genes appear to be involved in its etiology. Epigenetic mechanisms such as microRNA-mediated gene regulation can determine the susceptibility and severity of autoimmune diseases. Therefore, to determine the role of miR-146a and miR-155 in MS and its developmental stages, the expression levels in the serum of MS and clinically isolated syndrome (CIS) patients were compared with those of healthy controls. In the present study, the expression levels of miR-146a and miR-155 were assessed using quantitative Real-Time PCR in blood samples of 15 CIS patients and 61 relapsing-remitting multiple sclerosis (RRMS) patients alongside 32 healthy patients as controls. Furthermore, any associations with the clinicopathologic variables of the patients were also evaluated. Dysregulations were found only in the miR-146a and miR-155 expressions in the RRMS-Control group. When the RRMS patients were evaluated in terms of the characteristics of sex, annual attack rate, age of diagnosis, duration of follow-up, and immunomodulatory treatments used, no significant differences were observed. However, significant dysregulations were identified in miRNA expression in the vitamin D level, EDSS values, and the number of attacks. ROC curve analysis showed that miR-146a and miR-155 were significant in the RRMS-Control group for the area under the curve (AUC). It is possible that miR-146a may be associated with vitamin D deficiency and disease disability, while miR-155 may be associated with the number of attacks.