Browsing by Author "Ulus, Ismail H."
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Publication Choline or cdp-choline restores hypotension and improves myocardial and respiratory functions in dogs with experimentally-induced endotoxic shock(Elsevier, 2021-10-27) Ozarda, Yesim; Ceron, Jose Joaquin; Buturak, Ali; Ulus, Ismail H.; Kocaturk, Meric; KOCATÜRK, MERİÇ; Yilmaz, Zeki; YILMAZ, ZEKİ; Cansev, Mehmet; CANSEV, MEHMET; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; 0000-0002-2849-1222; 0000-0001-9836-0749; 0000-0003-2918-5064; 0000-0002-8654-1793; H-9043-2015; D-5340-2015; V-5578-2017; A-9637-2008Endotoxin shock is associated with severe impairments in cardiovascular and respiratory functions. We showed previously that choline or cytidine-5 '-diphosphocholine (CDP-choline) provides beneficial effects in experimental endotoxin shock in dogs. The objective of the present study was to determine the effects of choline or CDPcholine on endotoxin-induced cardiovascular and respiratory dysfunctions. Dogs were treated intravenously (i.v.) with saline or endotoxin (LPS, 0.1 mg/kg) 5 min before i.v. infusion of saline, choline (20 mg/kg) or CDP-choline (70 mg/kg). Blood pressure, cardiac rate, myocardial and left ventricular functions, respiratory rate, blood gases, serum electrolytes and cardiac injury markers were determined before and at 0.5-48 h after endotoxin. Plasma tumor necrosis factor alpha (TNF-alpha), high mobility group box-1 (HMGB1), catecholamine and nitric oxide (NO) levels were measured 2 h and 24 h after the treatments. Endotoxin caused immediate and sustained reductions in blood pressure, cardiac output, pO2 and pH; changes in left ventricular functions, structure and volume parameters; and elevations in heart rate, respiratory rate, pCO2 and serum electrolytes (Na, K, Cl, Ca and P). Endotoxin also resulted in elevations in blood levels of cardiac injury markers, TNF-alpha, HMGB1, catecholamine and NO. In choline- or CDP-choline-treated dogs, all endotoxin effects were much smaller in magnitude and shorter in duration than observed values in controls. These data show that treatment with choline or CDP-choline improves functions of cardiovascular and respiratory systems in experimental endotoxemia and suggest that they may be useful in treatment of endotoxin shock in clinical setting.Item Choline status in newborns, infants, children, breast-feeding women, breast-fed infants and human breast milk(Elsevier Science, 2005-08) Özarda, Yeşim; Özbek, Resul; Hamurtekin, Emre; Ulus, Ismail H.; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi.; Uludağ Üniversitesi/Tıp Fakültesi/Farmakoloji ve Klinik Farmakoloji Anabilim Dalı.; AAL-8873-2021; D-5340-2015This study assessed the choline status in newborns, infants, children, breast-feeding women, breast milk, infant forinula, breast-fed and formula-fed infants. The serum free choline level was 35.1 +/- 1.1 mu mol/L at birth and decreased to 24.2 +/- 1.6, 18.1 +/- 0.8, 16.3 +/- 0.9, 14.3 +/- 0.8, 12.9+0.6 or 10.9 +/- 0.6 mu mol/L at 22-28, 151-180, 331-365, 571-730, 731-1095 or 4016-4380 days after birth, respectively. The serum phospholipid-bound choline level was 1997 +/- 75 mu mol/L at birth and increased gradually to 2315 +/- 190 or 2572 +/- 100 mu mol/L at 571-730 or 4016-4380 days after birth, respectively. In breast-feeding women, serum free and phospholipid-bound choline levels were doubled at 12-28 days after birth, they decreased toward the control values with time. Free choline, phosphocholine and glycerophosphocholine were major choline compounds in breast milk. Their concentrations in mature milk were much greater than in colostrum and serum. Choline contents of breast milk varied greatly between mothers, and milk free choline levels were correlated with serum free choline (r.541; P <.001), phospholipid-bound choline (r=.527; P <.001) and glycerophosphocho line (r=.299-1 P <.01) concentrations and lactating days (r=.520 - P <.001). In breast-fed infants, serum free choline concentrations were correlated with free choline (r.47; P <.001), phosphocholine (r=.345; P <.002), glycerophosphocholine (r=.311; P <.01) and total choline (r=.306; P <.01) contents of breast milk. Serum free choline concentration in formula-fed infants was lower than breast-fed infants. These data show that (a) circulating choline status is elevated during infancy and lactation, (b) choline contents of breast milk vary between mothers and milk free choline contents are influenced by maternal circulating choline status, and (c) the choline contents of breast milk can influence infants' circulating choline status.