Browsing by Author "Wako, T."

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    Development of EST based SSR markers for garlic genome
    (Int Soc Horticultural Science, 2012) Simon, Philipp W.; Wako, T.; İpek, Meryem; İpek, Ahmet; Cansev, Asuman; Şeniz, Vedat; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-3353-846X; AAH-3233-2021; AAH-4255-2019; 16031208900; 6603912487; 26326677200; 13604787100
    Although it is time consuming and expensive to develop SSR markers, they have some advantages such as co-dominancy, reproducibility and high amount of polymorphic alleles as a PCR based marker system. For genetic and molecular studies in garlic, generally RAPD and AFLP markers have been utilized. However, development and use of SSR markers have been limited to few studies. In order to develop detailed genetic map and genetic studies, co-dominant marker systems like SSRs and SNPs are necessary in garlic. The purpose of this study was the development of SSR markers from expressed sequence tags (ESTs) derived from the genome of garlic. The SSR motifs in EST sequences were screened and it was revealed that SSR motifs are abundant in garlic ESTs. So far six SSR markers have been developed. EST based SSR markers could be used to map genes to garlic genetic maps directly and assessment of genetic diversity for the expressed regions of the garlic genome.
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    Differentially expressed genes in leaf, meristematic and flower tissues of garlic
    (Int Soc Horticultural Science, 2012) Senalik, Douglas A.; Simon, Philipp W.; Wako, T.; İpek, Meryem S.; İpek, Ahmet; Aydoğan, Çiğdem; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0003-4884-5304; AAH-3233-2021; AAG-6532-2020; 16031208900; 6603912487; 55481474200
    Differential gene expression analysis has been investigated to reveal important genes in many plant species. Identification of genes controlling metabolic pathways in garlic could contribute to the understanding of garlic genetic. In this study, transcript (mRNA) profile of differentially expressed genes was determined in leaf, meristematic and flower tissues of three genetically divers garlic clones at different developmental stages using cDNA-AFLP approach. In total, 352 differentially expressed transcript-derived fragments (TDFs) were evaluated from 20 primer combinations. While seven TDFs fragments expressed only in meristematic tissues, 30 TDFs expressed only in leaf tissues. Twenty seven fragments expressed in both meristematic and leaf tissues with intensity polymorphism and two fragments differentially expressed in flower tissues. BLAST analysis of these tissue specific expressed fragments revealed that some genes have significant similarities with previously determined sequences in the database of NCBI GenBank while some of them are unique to garlic genome. These tissue specific expressed genes can be used to develop tissue specific markers for garlic genome.
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    Onion breeding program in Turkey
    (Int Soc Horticultural Science, 2012) Başar, N.; Çandar, Ayşe; Wako, T.; Gökçe, Ali Fuat; Kaderlioğlu, E.; Akbudak, Nuray; Uludağ Üniversitesi/Ziraat Fakültesi/Bahçe Bitkileri Bölümü.; 0000-0002-2953-0326; A-7818-2018; AAH-5045-2021; 6603693762; 55547805900; 13605815800
    The bulb onion (Allium cepa L.) has been cultivated for thousands of years and is broadly dispersed over Turkey, Iran, Iraq, Afghanistan, Kazakhstan, and western Pakistan. Onion is one of the earliest produced and consumed crops in Turkey and is used daily in cooking by all Turkish families year-round. In addition to consumption as food, onion and its relatives are still used in remote villages of Turkey to cure or enhance some health problems such as asthma, bolting, fertility, infections, high blood pressure, high fever, kidney stone, parasite, and hemorrhoid. The edible Alliums are grown worldwide and have been historically maintained as open pollinated populations and are grown as fresh shoots for green salad onions and as bulbs to consume as fresh, pickled, dehydrated, cooked, or to produce onion seed or sets. Turkey produces approximately 3% of the world onion production. Our breeding achievements of onion in Turkey are better quality, high yield, uniformity, resistance to diseases, bulb size, shape, color, pungency, single center, firmness, tightness of scale and neck, dormancy, amount of soluble solids, earliness for harvest, anthocyanins, and antioxidant activities. We had improvements in reducing split or multiple centered bulbs rates, increasing earliness and uniformity at harvest, firmness, scale and neck tightness, anthocyanins and antioxidant activities. Our onion breeding program from 2002 to 2010 in Turkey will be discussed at the presentation.