Browsing by Author "YERCİ, ÖMER"
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Publication Cancer stem cell markers in pancreatic ductal adenocarcinoma(Oxford Univ Press, 2018-10-01) Aksoy, Fuat; Kaya, Ekrem; Egeli, Ünal; Dündar, Halit Ziya; Taşar, Pınar; Aksoy, Seçil; Özen, Yılmaz; Tunca, Berrin; Çeçener, Gülşah; Yerci, Ömer; AKSOY, FUAT; KAYA, EKREM; EGELİ, ÜNAL; DÜNDAR, HALİT ZİYA; TAŞAR, PINAR; AKSOY, SEÇİL; ÖZEN, YILMAZ; TUNCA, BERRİN; ÇEÇENER, GÜLŞAH; YERCİ, ÖMER; 0000-0002-9562-4195; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Bölümü; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Biyoloji Bölümü; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Bölümü; 0000-0001-5808-9384; 0000-0001-7904-883X; 0000-0002-1619-6680; 0000-0002-3820-424X; 0000-0002-3820-424X; AAH-1420-2021; AAH-3847-2021; ADM-8457-2022; HII-8895-2022; ABI-6078-2020; AAG-7319-2021; EWI-3634-2022; IIC-9825-2023; FOQ-1792-2022; AAP-9988-2020Publication Do morphologic characteristics play a role in nodal metastatic progression of well-differentiated pancreatic neuroendocrine tumors?(Sage Publications Ltd, 2020-07-24) Hasdemir, Seçil; HASDEMİR, SEÇİL; Ugraş, Nesrin; UĞRAŞ, NESRİN; Yerci, Omer; YERCİ, ÖMER; Tasar, Pınar; TAŞAR, PINAR; Dundar, Halit Ziya; DÜNDAR, HALİT ZİYA; Macunluoğlu, Aslı Ceren; Bursa Uludağ Üniversitesi/Tıp Fakültesi/ Genel Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/ Bioistatistik Anabilim Dalı.; 0000-0003-1769-7484; 0000-0002-6802-5998; AAH-2716-2021Background: Pancreatic neuroendocrine neoplasms (NENs) are tumors with histopathologic and prognostic heterogeneity that pose difficulties in establishing standards for diagnosis, classification, and treatment. Among NENs, well-differentiated neuroendocrine tumors (NETs) have been classified as grade 1, 2, and 3 in the most recently released World Health Organization classification. Although well-differentiated NETs are associated with relatively better prognosis, they have a potential for malignant behavior such as extrapancreatic spread, metastasis, or recurrence. The present study aimed to evaluate clinical and histomorphologic findings of patients with well-differentiated pancreatic NETs and to identify histopathologic findings effective in predicting nodal metastatic progression. Methods: The study group consisted of 54 patients diagnosed with well-differentiated NET. All preparations and blocks of the patients were examined for the following histopathologic parameters: tumor diameter, microscopic tumor growth pattern (solid, trabecular, acinar, and mixed), cellular features (clear, eosinophilic, oncocytic, peliotic, and pseudopapillary), stromal changes (calcification, lymphocytic infiltration, and stromal hyalinization), presence of necrosis, perineural invasion, lymphovascular invasion, mitotic activity, and Ki67 proliferative index. Results: Lymph node metastasis was present in 7 patients. Lymph node metastasis was significantly associated with tumor diameter of >2 cm (p= 0.012), Ki67 proliferative index of >20% (p= 0.022), grade 3 tumors (p= 0.002), presence of dense stromal hyalinization (p= 0.034), and mild lymphocytic infiltration (p= 0.041). Conclusion: The present study revealed that the new findings such as presence of dense stromal hyalinization and absence of remarkable lymphocytic infiltration could be predictive morphologic findings for the development of lymph node metastasis.Publication Early-stage colon cancer with high malat1 expression is associated with the 5-fluorouracil resistance and future metastasis(Springer, 2022-07-06) Öztürk, Ersin; Aksoy, Seçil Ak; Tunca, Berrin; TUNCA, BERRİN; Erçelik, Melis; Tezcan, Gulcin; TEZCAN, GÜLÇİN; Çeçener, Gülşah; ÇEÇENER, GÜLŞAH; UĞRAŞ, NESRİN; YILMAZLAR, AHMET TUNCAY; Yerci, Omer; YERCİ, ÖMER; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-1619-6680; 0000-0002-5956-8755; 0000-0001-8593-5101; 0000-0002-3820-424X; ADM-8457-2022; AAH-3843-2020Background This study aimed to investigate the role of long noncoding RNA (LncRNA) expression profiles to predict relapse and 5-FU response in patients with stage I/II colon cancer (CC). Methods and Results The expression level of 15 LncRNA was analyzed in stage I/II colon tumors of 126 CC patients. To confirm the findings in-vitro, 5FU-resistant HT29 cells were generated by subjecting HT-29 cells to the increasing concentrations of 5FU for 6 months. The 5FU resistance was observed in WST-1 and Annexin V analyses. The colony formation and wound healing assays were assessed to determine the metastatic properties of the cells. Expression levels of LncRNAs and mRNA of EMT-related genes were determined by RT-PCR. The role of LncRNA on metastasis and 5FU sensitivity were confirmed in pcDNA3.0-PTENP1 and si-MALAT1 expressed 5FU-resistant HT29 cell lineages. Results High MALAT1 (p = 0.0002) and low PTENP1 (p = 0.0044) expressions were significantly associated with 5-FU resistance and tumor relapse in stage I/II CC. The invasiveness and colony-forming characteristics of 5-FU-resistant cell lineages were higher as compared to the parent HT-29. Moreover, the expression of MALAT1 (p = 0.0009) was increased while the expression of PTENP1 (p = 0.0158) decreased in 5FU-resistant-HT-29 cells. Si-MALAT1 treatment increased cell sensitivity to 5FU, whereas it decreased invasive behaviors of 5 FU-resistant-HT-29 cells. Conclusion MALAT1 may be a biomarker in predicting recurrence in early-stage CC. Our findings suggest that a cell-based therapy to target MALAT1 could be established for these patients to prevent metastasis and 5-FU resistance.Publication Evaluation of cystic lesions of the pancreas based on clinicopathologic parameters(Carbone Editore, 2015-01-01) Atalay, Fatma Öz; Uğras, Nesrin; Aytaç, Berna; Yerci, Ömer; ÖZ ATALAY, FATMA; UĞRAŞ, NESRİN; AYTAÇ VURUŞKAN, BERNA; YERCİ, ÖMER; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; AAH-9746-2021; AAH-2716-2021; JHC-4482-2023; EGD-8703-2022Aims: To identify demographic, histopathologic and immunohistochentical features of pancreatic cystic lesions, which could assist diagnosis of neoplastic ones.Materials and methods: Sixty-seven cystic lesions from pancreatic resections between 2001 and 2013 were studied. Patient demographics and cyst location and size were recorded. Forty-sir specimens with adjacent non-lesional parenchyma were evaluated for pancreatic intraepithelial neoplasia (PanIN). Neoplastic cysts were immunohistochemically tested for ki67, p53, and progesterone receptor. Ten high-power fields were examined to determine ki67 proliferative index (PI; average percentage of nuclear staining) and p53 status (<1% stained nuclei indicating negative). Presence/absence of progesterone receptor (PR)-positive nuclei in pericystic stromal cells Was recorded.Results: Eighteen (26.9%) of the 67 lesions were non-neoplastic and 49 (73.1%) were neoplastic. Serous cystadenomas (SCAs) were most common (n=23: 34.3%), and 59 (88%) of the patients were women. Mean ages for the mucinous cystic neoplasm (MCN) and SCA subgroups were 46.7 and 64.6 years, respectively, and these lesions tended to be in the pancreatic tail and head, respectively. ki67 PI was higher for non-invasive MCNs with high-grade dysplasia than low-grade dysplasia (20% vs. 4-6%, respectively). No neoplasms were p53-positive. Only MCNs were PR-positive. SCA was the lesion most frequently associated with PanIN.Conclusion: Age of patient and the location of cyst are distinctive features in neoplastic pancreatic cysts. Diameter of the cyst gives an idea about the malignancy potential of the lesions. In MCNs. considering the increased risk of multiple foci of varying degrees of dysplasia, ki67 proliferative index is useful as well as the adequate sampling of the cyst for the accurate diagnosis of these foci, particularly when the presence or degree of it cannot he adequately assessed in hematoxylin and eosin (HE)-stained sections.Publication Histopathological changes of neoadjuvant chemoradiation and relation with the pre-treatment tumor stage in rectal carcinoma(Gazi Univ, Fac Med, 2017-01-01) Özgün, Gonca; ÖZ ATALAY, FATMA; Ugraş, Nesrin; UĞRAŞ, NESRİN; Yerci, Ömer; YERCİ, ÖMER; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-7188-6115; AAH-2716-2021Objective: Neoadjuvant chemoradiotherapy has considered one of the standard treatment modalities for locally advanced rectal cancers. Chemoradiotherapy has a variety of different effects and responses on tumor, tumor bed and peritumoral tissues. The purpose of the present study was to evaluate the stromal responses in tumor bed between the different treatment modalities and different clinical T stages.Methods: Fifty-seven consecutive patients with median age of 62.4 years were treated for rectal adenocarcinoma between January 2005 and July 2012 in Uludag University Medical Faculty. Twenty-three of the patients were treated with neoadjuvant chemoradiation therapy and following surgery, 34 patients treated with surgery only.Results: When we compared the stromal responses in the tumor bed between the two different treatment modalities, the amount of fibrosis and intensity of inflammatory cell infiltration were found considerably marked. The existence of calcification, hemosiderin-laden macrophages and mucin lakes were found also significant marked. There was no difference found in between the patients with different clinical stages which were received neoadjuvant CRT.Conclusion: The stromal response in the tumor bed increases with the neoadjuvant chemoradiotherapy but the excess of the response doesn't have any relation with the clinical T stage.Publication Igg4-related disease of the ovary(Türk Patoloji Derneği, 2021-01-01) Akyol, Sevda; Öz Atalay, Fatma; Hasdemir, Seçil; Yerci, Ömer; AKYOL, SEVDA; ÖZ ATALAY, FATMA; HASDEMİR, SEÇİL; YERCİ, ÖMER; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı; 0000-0003-1769-7484; 0000-0002-3107-207X; 0000-0002-1275-7743; 0000-0001-7118-5258; CBY-0641-2022; FUC-3875-2022; GBY-7549-2022; EIS-5114-2022Immunoglobulin G4-related disease is characterized by dense fibrosis, obliterative phlebitis, and lymphoplasmacytic infiltration that contains abundant IgG4 positive plasma cells. It causes tumefactive lesions in the involved organs and is most commonly seen in the salivary glands, pancreas, and retroperitoneum. Ovarian involvement has been reported in only two cases. In our case, a 58-year-old female patient presented with abdominal distention and pain. Pelvic computed tomography revealed a soft tissue lesion compatible with the omental cake, several intraabdominal implants, and bilateral adnexal fullness. A laparotomy was performed under suspicion of peritoneal carcinomatosis secondary to bilateral adnexal mass. In the histopathologic examination, abundant lymphoplasmacytic infiltration and dense fibrosis were observed in both ovaries and the peritoneum. In the areas of greatest density, the density of IgG4-positive plasma cells was found to range from 40 to 50 per high-power field. The patient was accepted as suffering from probable IgG4-related disease because of the bilateral involvement of the ovaries and the histopathological findings. In conclusion, we present this case to draw attention to the fact that IgG4-related disease can also be seen in the ovary.Publication Investigation of apc mutations of a patient with fap and her family members by heterodublex analyses(Nature Publishing Group, 2002-05-01) Menigatti, M; Benatti, P; Pedroni, M; Scarselli, A; Borghi, F; Sala, E; Yerci, O; de Leon, MP; TUNCA, BERRİN; Çeçener, Gülşah; YILMAZLAR, AHMET TUNCAY; ÇEÇENER, GÜLŞAH; Yılmazlar, Tuncay; Zorluoglu, A; EGELİ, ÜNAL; Yerci, Ömer; YERCİ, ÖMER; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Medikal Biyoloji ve Genetik Anabilim Dalı.; 0000-0002-1619-6680; 0000-0002-3820-424X; 0000-0001-7904-883X; AAH-1420-2021; ABI-6078-2020Publication Investigation of cerbb2 at mrna level in patients with gastric adenocarcinoma(Bayrakol Medical Publisher, 2022-06-01) Turhan, Ezgi Işıl; Aksoy, Seçil Ak; UĞRAŞ, NESRİN; IŞIK, ÖZGEN; AKSOY, SEÇİL; TUNCA, BERRİN; Tunca, Berrin; Işık, Özgen; Yerci, Ömer; YERCİ, ÖMER; Vuruşkan, Berna Aytaç; AYTAÇ VURUŞKAN, BERNA; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Klinik Biyoloji Anabilim Dalı.; 0000-0002-1619-6680; 0000-0002-9541-5035; ADM-8457-2022; AAW-9602-2020Aim: Gastric adenocarcinomas take place near the top regarding mortality due to cancer. This study aims to validate IHC results with the RT-PCR method and to evaluate their contribution to confirm the absolute score of CerbB2 situation in tissues. Material and Methods: We analyzed 80 gastric adenocarcinoma cases diagnosed in our clinic. The expression characteristics of the cases were evaluated using CerbB2 staining. Simultaneously, CerbB2 expression analyses were performed with the RT-PCR method. Results: Positive immunoreactivity was observed in 19 of 80 cases (23,75%) in the study conducted using CerbB2 antibody. While 14 of these cases (17.5%) demonstrated weak positive (2+), 5 of them (6.25%) demonstrated strong positive (3+) immunoreactivity. With the RT-PCR method, an increase in gene expression was observed in 12 of 14 weak positive cases (75%). In all 5 strong positive cases, on the other hand, high gene expression was determined. Between CerbB2 immunohistochemical findings and gene expression, 89% compatibility and a statistically significant relationship was determined. While an increase in CerbB2 gene expression, determined by Real Tirme PCR method, was not observed in 57 of 61 cases (96.6%) without CerbB2 immunoreactivity, 4 cases had an increase in CerbB2 expression in comparison to normal. In our study, a statistically significant relationship was determined between CerbB2 expression and Helicobacter pylori (H. pylori) that is a factor blamed for gastric cancer etiology. The relationship between CerbB2 expression and clinicopathologic prognostic factors has been statistically reviewed; no significant results have been found. Discussion: Our study has shown that RT-PCR is a method, which might be an alternative to IHC and in ISH methods. It is concluded that a statistically significant relationship might be determined between CerbB2 expression and clinicopathologic prognostic parameters by increasing the case number.Publication Ki-67 proliferation index in renal biopsy samples of patients with systemic lupus erythematosus(Clinical, 2015-05-01) Dalkılıç, Ediz; Pehlivan, Yavuz; Ermurat, Selime; Coşkun, Belkıs Nihan; Tufan, Ayşe Nur; Öksüz, Mustafa Ferhat; Yerci, Ömer; Güllülü, Mustafa; DALKILIÇ, HÜSEYİN EDİZ; PEHLİVAN, YAVUZ; Ermurat, Selime; COŞKUN, BELKIS NİHAN; Tufan, Ayşe Nur; Öksüz, Mustafa Ferhat; YERCİ, ÖMER; GÜLLÜLÜ, MUSTAFA; Uludağ Üniversitesi/Tıp Fakültesi/Romatoloji Bölümü; Uludağ Üniversitesi/Tıp Fakültesi/Patholoji Bölümü; AAG-8227-2021; AAG-7155-2021; ABE-4424-2022; CMF-4757-2022; GHE-4236-2022; JXF-7598-2024; GKD-6344-2022; CTG-8811-2022Publication Malignant glomus tumor with oncocytic features: An unusual presentation of dysphagia(Wiley, 2015-07-01) Uğras, Nesrin; Yerci, Ömer, I.; Yalçınkaya, Ulviye; Gülcü, Barış; Öztürk, Ersin; Yıldırım, Çınar; Çavuşoğlu, İlkin; UĞRAŞ, NESRİN; YERCİ, ÖMER; YALÇINKAYA, ÜLVİYE; Gülcü, Barış; Öztürk, Ersin; Yıldırım, Çınar; Çavuşoğlu, İlkin; Uludağ Üniversitesi/Tıp Fakültesi/Cerrahi Patoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Gastroenteroloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-9600-9418; AAH-8924-2021; AAH-2716-2021; JIO-2609-2023; JII-0241-2023; JGW-0566-2023; GCS-6830-2022; JIV-8745-2023Glomus tumors in the gastrointestinal tract are unusual, as the previous series in the literature have been mainly limited to the stomach. Less than 10 cases of esophageal glomus tumors have been described in the literature. Oncocytic glomus tumors are a recently identified, rare variant of the glomus tumor. We report a 47-year-old female who presented with an approximately 3-month history of dysphagia and weight loss. Upper gastrointestinal endoscopy showed a black-purple, hypervascular, protruding lesion measuring approximately 65mm at the 37th cm of the esophagus. The patient underwent an Ivor Lewis operation via open thoracotomy. The resected specimen had a protuberant, ulcerated mass measuring 80x35mm in the posterior wall of the esophagus. Based on the histopathological, immunohistochemical and electron microscope findings, the final diagnosis was a malignant glomus tumor with oncocytic features. To our knowledge, this is the first report of a malignant glomus tumor with oncocytic features in an esophageal location.Publication MicroRNA-21 functions as a prognosis predictor in head of pancreas tumor(Oxford Univ Press, 2018-03-01) Aksoy, Fuat; Aksoy, Savaş; Tunca, Berrin; Dündar, Halit Ziya; Sarkut, Pınar; Özen, Y.; Egeli, Ünal; Çeçener, Gülşah; Yerci, Ömer; Kaya, Ekrem; AKSOY, FUAT; Aksoy, Savaş; TUNCA, BERRİN; DÜNDAR, HALİT ZİYA; Sarkut, Pınar; Özen, Y.; EGELİ, ÜNAL; ÇEÇENER, GÜLŞAH; YERCİ, ÖMER; KAYA, EKREM; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Bölümü; Bursa Uludağ Üniversitesi/Tıp Fakültesi; 0000-0001-5808-9384; 0000-0002-1619-6680; 0000-0001-7904-883X; 0000-0002-3820-424X; 0000-0002-9562-4195; AAH-3847-2021; HII-8895-2022; AAH-1420-2021; ABI-6078-2020; CAV-8845-2022; EWI-3634-2022; HKB-5363-2023; FOQ-1792-2022; AAP-9988-2020; AAG-7319-2021Publication The diagnostic value of endoscopic narrow band imaging in helicobacter pylori gastritis in children(Aves, 2015-03-01) Özgür, Taner; Özkan, Tanju Başarır; Erdemir, Gülin; Özakın, Cüneyt; Yerci, Ömer; ÖZGÜR, TANER; Özkan, Tanju Başarır; Erdemir, Gülin; ÖZAKIN, CÜNEYT; YERCİ, ÖMER; Uludağ Üniversitesi/Tıp Fakültesi/Çocuk Gastroenteroloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji Anabilim Dalı.; Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-9726-8219; 0000-0001-5740-9729; 0000-0001-5428-3630; AAG-8381-2021; AAG-8392-2021; DKU-2575-2022; GBW-0079-2022; EIS-5114-2022Background/Aims: In this study we aimed to investigate the sensitivity and specificity of Narrow Band Imaging (NBI) in H. pylori gastritis and compare them with those of rapid urease test and urea breath test.Materials and Methods: A hundred sixty-five children who admitted to Uludag University Pediatric Gastroenterology Unit between October 2009-March 2011 with upper gastrointestinal symptoms were consecutively enrolled. During the endoscopy procedure gastric corporeal, antral and fundal images were obtained, afterwards the same areas were visualized with narrow band imaging and images were recorded again.Results: The study included 68 (41.2%) boys and 97(58.8%) girls. The mean age of the patients were 11.88 +/- 4.55. Tissue culture positivity and/or histopathological staining for H. pylori was determined in 56 (33.9%) patients (Group 1) and the other patients (n:109, 43.6%) didn't have an evidence of H. pylori infection (Group 2). Narrow band images have supported H. pylori infection in 56.4%. The sensitivity of narrow band images for determining H. pylori infection was 92.86% (95% CI 82.7-98), specificity was 62.39% (95% CI 52.6-71.5).Conclusion: Our study is the first to show the role of NBI in diagnosing H. pylori infection in children, as well as determining the sensitivity and specificity of the technique. The specificity is low; however, we suggest that the specific mucosal view of H. pylori gastritis provided by NBI is useful for identifying the areas from which the biopsies should be taken. Moreover, by using this technique, treatment of H. pylori infection may be initiated immediately without performing rapid urease test and without waiting for histopathology report and tissue culture.