Browsing by Author "Yasemin, Sara"

Now showing 1 - 2 of 2

Effects of disinfection conditions and culture media on in vitro germination of sea daffodil (Pancratium maritimum)
(Uludağ Üniversitesi, 2017-04-13) Yasemin, Sara; Köksal, Nezihe; Büyükalaca, Saadet
In this study, we investigated the disinfection methods and germination conditions for seeds of sea daffodil (Pancratium maritimum). Seeds were exposed to 20% and 40% NaOCl (Sodium hypochlorite) solutions for 15, 20, and 25 minutes, as disinfection treatments. The rate of 20% NaOCl was the most appropriate concentration for percentage of seed germination (58%) and plant formation (34%). Effects of three culture media on seed germination, plant formation, and growth were investigated: A. Agar (4 gl-1 ) with sucrose (30 gl-1 ); B. MS (Murashige - Skoog) with agar (4 gl-1 ) and sucrose (30 gl-1 ); C. MS with agar (4 gl-1 ), sucrose (30 gl-1 ), and plant growth regulators (1 mgl-1 BA, 0.1 mgl-1 2,4-D). Evaluated plant growth parameters were total plant weight; number, width, and length of leaf; thickness and length of root; and diameter, length, and weight of bulb. All culture media were found to be successful in terms of in vitro germination of the seeds. The most suitable medium for plant formation and all the plant growth parameters were found to be Medium B. This is an innovative report in that it has been thoroughly investigated in terms of disinfection practices and seed germination media with different evaluation parameters.
Growth and physiological responses of ‘Chrysanthemum paludosum’ under salinity stress
(Uludağ Üniversitesi, 2017-06-20) Yasemin, Sara; Köksal, Nezihe; Özkaya, Aslıhan; Yener, Mithat
Chrysanthemum paludosum, belongs to Asteraceae family, is a perennial medicinal herb and ornamental plant. It has aesthetical values together with several medical effects such as antibacterial, antifungal, insecticidal, antioxidant, nervous, cytotoxic etc. Excess of salinity in soils is one of the major problems which reduce plant growth. The purpose of this study was to determine the effects of salinity on C. paludosum by assaying plant growth and some physiological traits. C. paludosum plants were irrigated with five different levels of NaCl concentrations (0, 50, 100, 150, 200 mM) for 30 days with 3 days intervals in pots under greenhouse conditions. The effects of salinity stress on diameter of flower and disc floret, number of flowers, shoot height, root length, thickness of root collar and stem, fresh weights of root and shoot, dry weights of root and shoot, leaf chlorophyll concentration (SPAD readings), moisture content on wet basis, relative water content and ion leakage were investigated. According to the results, flower diameters, disc florets, number of flowers, shoot height, root collar thickness, root and shoot fresh weights were negatively affected in 150 and 200 mM NaCl treatments. In parallel, ion leakage also highly increased in 150 and 200 mM NaCl treatments. Leaf chlorophyll concentrations decreased by increasing salinity levels.