Browsing by Author "Yonkova, Penka Yonkova"
Now showing 1 - 1 of 1
- Results Per Page
- Sort Options
Item Role of tissue lipoprotein lipase activity localization in the pelvic urethra in male cats(Uludağ Üniversitesi, 2010-06-24) Dimitrov, Rosen Stefanov; Yonkova, Penka Yonkova; Stamatova, Kamelia DimchevaThe pelvic urethra has been investigated in seven sexually mature, clinically healthy European shorthair male cats, aged 12-18 months, weighing 2.8-4 kg, obtained from a licensed animal breeder. All experiments were carried out under strict observance of the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes and law on Animal Protection in the Republic of Bulgaria. Cryostat cross sections of 5-7 µm were used for detection of lipoprotein lipase (LPL) by Tween method of Gomori. The reaction was positive when clusters of dark-brown lead sulfide precipitates were present. The localization of tissue LPL expression was determined by light microscopy. The intensity of the reaction was assessed by a semi-quantitative analysis using the score system. The enzyme histochemical investigation of feline pelvic urethra showed that the highest intenstiy of LPL expression, occurred in the muscular layer that was mainly of skeletal muscle. A medium intensity was observed in epithelial cells of disseminated part of the prostate and in the lumen of its glandular tubules. The urethral propria exhibited weak LPL expression, whereas no LPL activity was detected in the urethral lumen epithelium