Browsing by Author "Yumuşak, Ezgi"
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Publication Evaluation of monocytic/granulocytic cells from spleen and liver in the experimental autoimmune encephalomyelitis mouse model(Wiley, 2021-08-01) Karaçay, Mehmet; Arslan, Gözde; Bayram, Gökçen Güvenç; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; Ermiş, Diğdem Yöyen; Akkoç, Ahmet; Yalçın, Murat; Oral, Haluk Barbaros; Karacay, Mehmet; Arslan, Gozde; Dombaz, Fatma; Etgu, Onur; Yumusak, Ezgi; YÖYEN ERMİŞ, DİĞDEM; AKKOÇ, AHMET; YALÇIN, MURAT; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; 0000-0001-7288-3250; 0000-0002-5090-7917; 0000-0002-5600-8162; 0000-0003-0463-6818; JFS-2013-2023; K-7285-2012; HKW-7185-2023; AAG-6956-2021; CXY-4200-2022; DWR-5356-2022; CPT-2053-2022; GYL-2038-2022; DTZ-3578-2022Publication Evaluation of some blood parameters and percentage of CD4+or CD8+T cells from spleen and liver from the experimental autoimmune encephalomyelitis mouse model(Wiley, 2021-08-01) Arslan, Gözde; Karacay, Mehmet; Bayram, Gökçen Güvenç; Özoğlu, Efe; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; Ermiş, Diğdem Yöyen; Akkoç, Ahmet; Yalçın, Murat; Oral, Haluk Barbaros; Arslan, Gözde; Karaçay, Mehmet; ÖZOĞLU, EFE; Dombaz, Fatma; Etgü, Onur; Yumuşak, Ezgi; YÖYEN ERMİŞ, DİĞDEM; AKKOÇ, AHMET; YALÇIN, MURAT; ORAL, HALUK BARBAROS; Bursa Uludağ Üniversitesi/Sağlık Bilimleri Enstitüsü.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/İmmünoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Fizyoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı.; 0000-0001-7288-3250; 0000-0002-5090-7917; 0000-0002-5600-8162; 0000-0003-0463-6818; K-7285-2012; AAG-6956-2021; JFS-2013-2023; HKW-7185-2023; CXY-4200-2022; FSU-7707-2022; DWR-5356-2022; JIJ-1849-2023; GYL-2038-2022; DTZ-3578-2022Publication Isolation and culture of chicken embryonic synovial fibroblasts(Ist Zooprofilattico Sperimentale Abruzzo & Molise G Caporale-izs A&m, 2023-01-01) Dayı, Belma; Sevimli, Alper; Akkoç, Ahmet; AKKOÇ, AHMET; Yumuşak, Ezgi; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; 0000-0002-7773-3419Cells obtained from chicken embryos are often preferred for in vitro studies. These cells, which easily adapt to rapid and continuous growth in the appropriate cell culture environment, are thought to be one of the effective methods in the investigation of leg diseases that are frequently observed in poultry. Leg diseases, especially affecting the joints in chickens, cause locomotor problems and adversely affect animal welfare. In addition, they cause significant health problems and increase mortality. It is known that synovial fibroblasts play an important role in joint diseases. In this study, chicken embryonic synovial fibroblasts were isolated from tissue explants taken from the tibio-metatarsal joint region of brown layer chicken embryos. Characterization of cells was evaluated by immunocytochemistry and hemacolor staining. chicken embryonic synovial fibroblasts showed a strong positive reaction to the vimentin antibody. As a result of hemacolor staining, it was noted that the cell morphology was spindle-shaped. The absence of macrophages in chicken embryonic synovial fibroblast culture was confirmed by the carbon powder uptake. In this present study, we aim to present a useful cell culture protocol such as primary culture, passage, and characterization suitable for chicken embryonic synovial fibroblast to be used in the new scientific research.