Browsing by Author "Zorlu, Yunus"

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Affinity of a new copper(II) complex to DNA/BSA and antioxidant/radical scavenging activities: Crystal structure of [Cu(4,7-diphenyl-1,10-phenanthroline)(leucine)(NO3)(H2O)]
(Taylor & Francis, 2016) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; G-2201-2019; AAH-8936-2021; 55082306300; 56261495600
A new copper(II) complex, [Cu(Bphen)(Leu)(NO3)(H2O)] (Bphen=4,7-diphenyl-1,10-phenanthroline, leu=L-leucine), has been synthesized and characterized by IR spectroscopy, CHN analysis, and single-crystal X-ray diffraction techniques. The CT-DNA binding properties of the complex have been investigated by both absorption and emission spectroscopy. The binding parameters for the fluorescence Scatchard plot were also determined. Further, the interaction of the complex with bovine serum albumin (BSA) has been investigated using absorption and emission spectroscopy. The thermodynamic parameters, free energy change (G), enthalpy change (H), and entropy change (S), were calculated by the van't Hoff equation and discussed. The distance between BSA and the complex has been obtained according to fluorescence resonance energy transfer. Conformational changes of BSA have been observed from synchronous fluorescence. Antioxidant and radical scavenging activities of the complex were determined by various in vitro assays such as 1,1-diphenyl-2-picryl-hydrazyl free radicals (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS(+)), and reducing ability determination by H2O2 scavenging methods.
Antiproliferative activity of copper(II) glutamine complexes with N,N-donor ligands: Synthesis, characterization, potentiometric studies and DNA/BSA interactions
(Elsevier, 2019-05-21) Zorlu, Yunus; Kiraz, Sirem; İnci, Duygu; Aydin, Rahmiye; Vatan, Özgür; Cavaş, Tolga; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0003-1620-1918; 0000-0002-7687-3284; 0000-0002-0483-9642; 0000-0003-4944-0181; AAH-8936-2021; AAH-3508-2021; O-7508-2015; G-2201-2019
New water soluble copper(II) complexes, - [Cu(phen)(gln)(H2O)]NO3 center dot H2O (1) and [Cu(dmphen)(gln)(H2O)]ClO4 (2) - (phen: 1,10-phenanthroline, dmphen: 4,7-dimethyl-1,10-phenanthroline, gln: L-glutamine), have been synthesized and characterized by CHN analysis, ATR-FT-MIR, ESI-MS and single-crystal X-ray diffraction techniques. Binary and ternary complexes of copper(II) with the selected ligands have been investigated using potentiometric methods in 0.1 M KCl aqueous ionic media at 298.2 K. The protonation constants of the selected ligands and the stability constants of the complexes 1 and 2 have been calculated from the potentiometric data using the "BEST" software package and the potentiometric results have been analyzed using the "SPE" software package. The binding interaction of the complexes with calf thymus DNA (CT-DNA) was investigated by electronic absorption and emission spectroscopic methods revealed that the complexes could interact with CT-DNA via a moderate intercalation mode. The fluorescence quenching mechanism of bovine serum albumin (BSA) by the complexes was analyzed and the binding constant has been calculated. In vitro antiproliferative effect of the complexes was examined on human tumor cell lines (Caco-2, A549 and MCF-7) and healthy cells (BEAS-2B). The complex 2 showed remarkable antiproliferative activity compared to the complex 1 and cisplatin.
Binary and ternary new water soluble copper(II) complexes of l-tyrosine and substituted 1,10-phenanthrolines: Effect of substitution on DNA interactions and cytotoxicities
(Pergamon-Elsevier, 2015-06-15) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Vatan, Özgür; Yılmaz, Dilek; Gençkal, Hasene Mutlu; Cavaş, Tolga; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; 0000-0002-0026-7755; 0000-0003-1620-1918; 0000-0002-7687-3284; 0000-0002-0483-9642; AAH-8936-2021; AAH-2888-2021; AAH-3508-2021; O-7508-2015; G-2201-2019; 55082306300; 56261495600; 16235098100; 6701369462; 57212275330; 6602989548
Binary and ternary water soluble copper(II) complexes - [Cu(nphen)(2)(H2O)](NO3)(2)center dot H2O (1), [Cu(phen)(2)(H2O)](NO3)(2) (2), [Cu(nphen)(L-tyr)(H2O)]NO3 center dot 2H(2)O (3), [Cu(Phen)(tyr)(H2O)] NO3 center dot 2H(2)O (4) - and diquarternary salts of nphen and phen (nphen = 5-nitro-1,10-phenanthroline, phen = 1,10-phenanthroline and tyr = L-tyrosine) have been synthesized and characterized by CHN analysis, H-1 NMR, C-13 NMR and IR spectroscopy, thermal analysis and single crystal X-ray diffraction techniques. The CT-DNA binding properties of these compounds have been investigated by thermal denaturation measurements, absorption and emission spectroscopy. The supercoiled pUC19 plasmid DNA cleavage activity of these compounds has been explored by agarose gel electrophoresis. The cytotoxicity of these compounds against MCF-7, Caco-2, A549 cancer cells and BEAS-2B healthy cells was also studied by using XTT method. The complexes 1-4 exhibit significant high cytotoxicity with low IC50 values in compared with cisplatin. The effect of the substituents of phen and coordinated amino acid in the above complexes are presented and discussed.
Biomacromolecular interactions and radical scavenging activities of one-dimensional (1D) copper(II) glycinate coordination polymer
(Springer, 2021-05-18) İnci, Duygu; Aydın, Rahmiye; Zorlu, Yunus; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; 0000-0003-4944-0181; AAH-8936-2021
One-dimensional (1D) copper(II) glycinate coordination polymer {[Cu(gly)(2)(H2O)](n); (gly: glycine)} has been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with biomacromolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes indicate that the complex binds to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between the complex and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (Delta G, Delta H, Delta S) of BSA + the complex systems were determined at different temperatures. The binding distance between the complex and BSA was calculated according to Forster non-radiation energy transfer theory (FRET). The effect of the complex on the conformation of BSA was also examined using synchronous, two-dimensional (2D) and three-dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complex was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interactions and radical activity studies, the complex was found to give good results.
Cu (II) tyrosinate complexes containing methyl substituted phenanthrolines: synthesis, x-ray crystal structures, biomolecular interactions, antioxidant activity, ros generation and cytotoxicity
(Wiley, 2019-01) Cosut, Bunyemin; Zorlu, Yunus; Inci, Duygu; Aydın, Rahmiye; Vatan, Ozgür; Hüriyet, Huzeyfe; Cinkılıç, Nilufer; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya, Anorganik Kimya Bölümü.; 0000-0002-0483-9642; 0000-0002-3595-6286; AAH-8936-2021; AAH-5296-2021; G-2201-2019; 55082306300; 56261495600; 16235098100; 57201093675; 26533892300
In this paper, three new copper (II) complexes, [Cu(4-mphen)(tyr)(H2O)]ClO4 (1), [Cu(5-mphen)(tyr)(H2O)]ClO4 center dot 1.5H(2)O (2) and [Cu (tmphen)(tyr)(NO3)]0.5H(2)O (3) (4-mphen: 4-methyl-1,10-phenanthroline, 5-mphen: 5-methyl-1,10-phenanthroline, tmphen: 3,4,7,8-tetramethyl-1,10-phenanthroline and tyr: L-tyrosine), were synthesized and characterized using elemental analyses, FT-IR, ESI-MS, cyclic voltammetry and single-crystal X-ray diffraction. It was found that the complexes adopt a distorted five-coordinate square pyramidal geometry. The interaction of the three complexes with calf thymus DNA was also investigated using UV-visible absorption spectra, ethidium bromide and Hoechst 33258 displacement assay and thermal denaturation. The DNA cleavage activity of the complexes, monitored using gel electrophoresis, showed significant damage of the pUC19 plasmid DNA. Binding activity of bovine serum albumin (BSA) reveals that these complexes can strongly quench the fluorescence of BSA through a static quenching mechanism. The results suggested that interaction of the complexes with DNA occurred through a partial intercalation into the minor grooves of DNA. In addition, interaction of the complexes with bovine serum albumin quenched the fluorescence emission of the tryptophan residues of the protein binding constants and thermodynamic parameters were obtained from the fluorescence quenching experiments at different temperatures. Free radical scavenging activities of the complexes were determined by various in vitro assays such as 1,1-diphenyl-2-picryl-hydrazyl free radicals (DPPH) and H2O2 scavenging methods. In addition, the cytotoxicity of these complexes in vitro on tumor cell lines (Caco-2 and MCF-7) was examined by XTT and showed better antitumor effect on the tested cells. ROS (reactive oxygen species) and comet experiments are consistent with each other and these complexes lead to DNA damage via the production of ROS. The effect of the hydrophobic properties of the synthesized complexes on DNA and BSA binding activities were discussed.
Cu(ii) complex with auxin (3-indoleacetic acid) and an aromatic planar ligand: Synthesis, crystal structure, biomolecular interactions and radical scavenging activity
(Springer, 2021-04-30) İnci, Duygu; Aydın, Rahmiye; Zorlu, Yunus; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; AAH-8936-2021
A novel water soluble ternary copper(ii) complex,-[Cu-2(phen)(2)(3-IAA)(2)(H2O)](ClO4)(2)center dot H2O-(phen: 1,10-phenanthroline, 3-IAA: 3-indoleacetic acid), has been synthesized and characterized by elemental CHN analysis, ESI-TOF, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with calf thymus DNA (CT-DNA) has been investigated by absorption spectral titration, ethidium bromide (EB) and Hoechst 33258 displacement assay. The interactions between the complex and bovine serum albumin (BSA) were investigated by electronic absorption and fluorescence spectroscopy methods. The experimental results indicate that the fluorescence quenching mechanism between the complex and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (Delta G, Delta H, Delta S) of BSA + complex systems were determined at different temperatures. The binding distance between the complex and BSA was calculated according to Forster non-radiation energy transfer theory (FRET). The effect of the complex on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Furthermore, the oxygen radical scavenging activity of the complex was determined in terms of IC50, using the DPPH and H2O2 method, to show that it particularly enables electron loss from radical species. This study highlights the importance of indole and moieties in the development of antioxidant agents.
Interaction of a new copper(II) complex by bovine serum albumin and dipeptidyl peptidase-IV
(Elsevier, 2018-09-28) Koseler, Aylin; Zeytunluoğlu, Ali; Zorlu, Yunus; Aydın, Rahmiye; İnci, Duygu; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0002-0483-9642; AAH-8936-2021; G-2201-2019; 55082306300; 56261495600
Dipeptidyl peptidase-IV (DPP-IV) is one of the mammalian serine proteases participated in the pathogenesis of diseases and DPP-IV inhibitors are now widely used as antidiabetic drugs. A new water soluble ternary copper (II) complex,-[Cu(PY-Phen) (phe) (H2O)]NO3 center dot H2O-(py-phen:pyrazino[2,3f][1,10]phenanthroline, phe:phenylalanine), has been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Fluorescence spectroscopy was researched to study the interaction between the complex and bovine serum albumin (BSA) and dipeptidyl peptidase-IV (DPP-IV). Chromophore of BSA and DPP-IV enzyme is changed upon addition of the complex. Additionally, the complex was shown to have promising inhibitory activities against DPP-IV with lower IC50 value. This study may provide new insights into the development of effective agents against diabetes.
Methyl substituent effect on one-dimensional copper(II) coordination polymers containing biologically active ligands: Synthesis, characterization, DNA interactions and cytotoxicities
(Wiley, 2019-07-24) Zorlu, Yunus; Şenel, Pelin; İnci, Duygu; Aydın, Rahmiye; Huriyet, Huzeyfe; Çinkılıç, Nilüfer; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; 0000-0003-4944-0181; 0000-0002-0483-9642; 0000-0002-3595-6286; 0000-0003-4495-8723; AAH-8936-2021; AAP-5959-2020; AAH-5296-2021; G-2201-2019; 55346251000; 55082306300; 57201093675; 26533892300; 56261495600
Three novel water-soluble copper(II) complexes - {[Cu(phen)(trp)]ClO4 center dot 3H(2)O}(n) (1), {[Cu(4-mphen)(trp)]ClO4 center dot 3H(2)O}(n) (2) and [[Cu(dmphen)(trp)(MeOH)][Cu(dmphen)(trp)(NO3)]]NO3 (3) (phen: 1,10-phenanthroline; 4-mphen: 4-methyl-1,10-phenanthroline; dmphen: 4,7-dimethyl-1,10-phenanthroline; trp: l-tryptophan) - have been synthesized and characterized using various techniques. Complexes 1 and 2 are isostructural, and exist as one-dimensional coordination polymers. Complex 3 consists of two discrete copper(II) complexes containing [Cu(trp)(dmphen)(MeOH)](+), [Cu(trp)(dmphen)(NO3)] and one nitrate anion. The binding interaction of the complexes with calf thymus DNA (CT-DNA) was investigated using thermal denaturation, electronic absorption and emission spectroscopic methods, revealing that the complexes could interact with CT-DNA via a moderate intercalation mode. The binding activity of the complexes to CT-DNA follows the order: 3 > 2 > 1. The pUC19 DNA cleavage activity of the complexes was investigated in the absence and presence of external agents using the agarose gel electrophoresis method. Especially, in the presence of H2O2 as an activator, the pUC19 DNA cleavage abilities of the complexes are clearly enhanced at low concentration. Addition of hydroxyl radical scavenger dimethylsulfoxide shows a marked inhibition of the pUC19 DNA cleavage activity of the complexes. In vitro cytotoxic effect of the complexes was examined on human tumor cell lines (Caco-2, A549 and MCF-7) and healthy cells (BEAS-2B). The potent cytotoxic effect of complex 3, with IC50 values of 1.04, 1.16 and 1.72 mu M, respectively, is greater relative to clinically used cisplatin (IC50 = 22.70, 31.1 and 22.2 mu M) against the Caco-2, A549 and MCF-7 cell lines.
New binary copper(II) complexes containing intercalating ligands: DNA interactions, an unusual static quenching mechanism of BSA and cytotoxic activities
(Taylor and Francis, 2017-11-18) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Vatan, Özgür; Çinkılıç, Nilüfer; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0483-9642; 0000-0003-4944-0181; 0000-0002-7687-3284; G-2201-2019; AAH-8936-2021; O-7508-2015; 55082306300; 56261495600; 16235098100; 26533892300
New binary copper(II) complexes - [Cu(4-mphen)(2)(NO3)]NO3 center dot H2O (1), [Cu(5-mphen)(2) (NO3)]NO3 center dot H2O (2), the known complex [Cu(dmphen)(2)(NO3)]NO3 (3) and [Cu(tmphen)(2) (NO3)]NO3 center dot H2O (4) - (4-mphen: 4-methyl-1,10-phenanthroline, 5-mphen: 5-methyl-1,10-phenanthroline, dmphen: 4,7-dimethyl-1,10-phenanthroline, tmphen: 3,4,7,8-tetramethyl-1,10-phenanthroline), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of these complexes with calf thymus DNA (CT-DNA) has been investigated by absorption spectral titration, ethidium bromide (EB) and Hoechst 33,258 displacement assay and thermal denaturation measurement. These complexes cleaved pUC19 plasmid DNA in the absence and presence of an external agent. Notably, in the presence of H2O2 as an activator, the cleavage abilities of these complexes are obviously enhanced at low concentration. Addition of hydroxyl radical scavengers like DMSO shows significant inhibition of the DNA cleavage activity of these complexes. BSA quenching mechanism was investigated with regard to the type of quenching, binding constant, number of binding locations and the thermodynamic parameters. The experimental results suggested that the probable quenching mechanism was an unusual static process and hydrophobic forces play a dominant role. The CT-DNA and BSA binding efficiencies of these complexes follow the order: 4 > 3 > 1 > 2. Furthermore, in vitro cytotoxicities of these complexes on tumor cells lines (Caco-2, MCF-7 and A549) and healthy cell line (BEAS-2B) showed that these complexes exhibited anticancer activity with low IC50 values. The effect of hydrophobicity of the methyl-substituted phenanthrolines on DNA and protein binding activities of these complexes is discussed.
New copper(ii) complexes containing tryptophan based schiff bases as promising antiproliferative agents on breast cancer cells
(Elsevier, 2023-12-28) Zorlu, Yunus; Arı, Ferda; ARI, FERDA; Gültekin, Büşra; Özbağcı, Duygu Inci; İNCİ ÖZBAĞCI, DUYGU; Aydın, İpek; İPEK, AYDIN; Aydın, Rahmiye; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Kimya Bölümü.; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0443-1129; 0000-0002-0483-9642; 0000-0003-4944-0181; 0000-0002-6729-7908; 0000-0003-2811-1872; G-2201-2019; IUO-8513-2023; AAG-7012-2021
Three new copper(II) complexes, [Cu(5-ClSal-Trp)(H2O)2] (1), [Cu(5-ClSal-Trp)(phen)] & sdot;C2H5OH (2) and [Cu (3,5-ClSal-Trp)(phen)] (3) (5-ClSal-Trp: Schiff base derived from 5-chlorosalicylaldehyde and L-tryptophan, 3,5-ClSal-Trp: Schiff base derived from 3,5-dichlorosalicylaldehyde and L-tryptophan, phen: 1,10-phenanthroline), have been synthesized and characterized by electronic absorption spectroscopy, CHN analysis, FTIR, ESI-MS and XRD techniques. Interaction of the complexes 1-3 with biomolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy. The results show that the complexes 1-3 can bind to CT-DNA via a moderate intercalation mode. Moreover, the fluorescence quenching mechanism between the complexes 1-3 and BSA is a static quenching process. Radical scavenging activity studies reveal that the complexes 1-3 show a moderate activity. Antiproliferative effects of the complexes 1-3 on both breast cancer cells (MCF-7 and MDA-MB-231) and healthy breast epithelial cells (MCF-10A) were also investigated using the Sulforhodamine B (SRB) viability assay. The results demonstrated that the complexes 1-3 exhibited a more pronounced cytotoxic effect on cancer cells compared to normal breast epithelial cells. Among the complexes, the best cytotoxic activity was obtained for the complex 3 against both human breast cancer cell lines. Further analysis indicated that the complex 3 induced apoptosis, as evidenced by fluorescent staining, positive Annexin-V-FITC staining, and the involvement of caspase. Subsequent to the administration of the complex 3, an evaluation of intracellular reactive oxygen species (ROS) generation was conducted through the utilization of dihydroethidium (DHE) fluorescent staining.
New water-soluble copper (II) complexes including 4,7-dimethyl-1,10-phenanthroline and l-tyrosine: Synthesis, characterization, DNA interactions and cytotoxicities
(Pergamon-Elsevier, 2015-02-05) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Yılmaz, Dilek; Gençkal, Hasene Mutlu; Vatan, Özgür; Çinkiliç, Nilüfer; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Anabilim Dalı.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Anabilim Dalı.; 0000-0002-0026-7755; 0000-0002-3595-6286; 0000-0002-7687-3284; 0000-0002-0483-9642; AAH-2888-2021; AAH-5296-2021; O-7508-2015; AAH-8936-2021; G-2201-2019; 55082306300; 56261495600; 6701369462; 57212275330; 16235098100; 26533892300
Two new water-soluble copper(II) complexes, [Cu(dmphen)(2)(NO3)]NO3 (1), [Cu(dmphen)(tyr)(H2O)] NO3 center dot H2O (2) and the diquartemary salt of dmphen (dmphen = 4,7-dimethyl-1,10-phenanthroline and tyr = L-tyrosine), have been synthesized and characterized by elemental analysis, H-1 NMR, C-13 NMR and IR spectroscopy, thermal analysis and single crystal X-ray diffraction techniques. The CT-DNA binding properties of these compounds have been investigated by absorption, emission spectroscopy and thermal denaturation measurements. The supercoiled pBR322 plasmid DNA cleavage activity of these compounds has been explored by agarose gel electrophoresis. The cytotoxicity of these compounds against MCF-7, Caco-2, A549 cancer cells and BEAS-2B healthy cells was also studied by the XTT method. Complexes 1 and 2 exhibit significant cytotoxicity, with lower IC50 values than those of cisplatin.
Newly synthesized Cu(II) pyrazino[2,3-f][1,10]phenanthroline complexes as potential anticancer candidates
(John Wiley and Sons, 2018-04) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Huriyet, Huzeyfe; Çinkılıç, Nilüfer; Uludağ Üniversitesi/Fen - Edebiyat Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0483-9642; 0000-0003-4944-0181; 0000-0002-3595-6286; G-2201-2019; AAH-8936-2021; AAH-5296-2021; CVI-9578-2022; 55082306300; 56261495600; 57201093675; 26533892300
New binary and ternary copper(II) complexes, [Cu(py-phen)(2)(NO3)]NO3 (1), [Cu-2(py-phen)(2)(gly)(2)(NO3)(2)(H2O)(2)]3H(2)O (2) and [Cu-2(py-phen)(2)(tyr)(2)(H2O)(2)](NO3)(2)3H(2)O (3) (py-phen: pyrazino[2,3-f][1,10]phenanthroline; gly: glycine; tyr: tyrosine), have been synthesized and characterized using CHN analysis, electrospray ionization mass spectrometry, Fourier transform infrared spectroscopy and single-crystal X-ray diffraction. Interaction of these complexes with calf thymus DNA has been investigated using absorption spectral titration, ethidium bromide and Hoechst 33258 displacement assay and thermal denaturation measurements. These complexes were found to be efficient cleaving agents and cleavage reactions were mediated by hydrolytic and oxidative pathways. The interaction between these complexes and bovine serum albumin (BSA) was investigated using electronic absorption and fluorescence spectroscopy. The experimental results show that the fluorescence quenching mechanism of these complexes and BSA is a static quenching process. Furthermore, in vitro cytotoxicities of these complexes against tumour cell lines (Caco-2, MCF-7 and A549) and healthy cell line (BEAS-2B) showed that they exhibited anticancer activity with low IC50 values. These complexes were markedly active against the cell lines and can be good drug candidates that are effective and safe for healthy tissue.
Noo-type tridentate schiff base ligand and its one-dimensional cu(ii) coordination polymer: Synthesis, crystal structure, biomacromolecular interactions and radical scavenging activities
(Elsevier, 2021-01-01) İnci, Duygu; Aydın, Rahmiye; Zorlu, Yunus; AYDIN, RAHMİYE; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü; AAH-8936-2021
N-salicylidene-2-amino5-chlorobenzoic acid, (H2L), NOO-type tridentate Schiff base and its one-dimensional Cu (II) coordination polymer, [Cu-2(H2L)(2)(MeOH)(n), have been synthesized and characterized by CHN analysis, FTIR, (HNMR)-H-1, C-13 NMR, ESI-MS and XRD techniques. Interaction of the H2L Schiff base ligand and its the Cu(II) coordination polymer with biomacramolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy. The experimental outcomes indicate that the H2L Schiff base ligand and its the Cu(II) coordination polymer bind to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between the H2L Schiff base ligand and its the Cu(II) coordination polymer and BSA is a static quenching process. Radical scavenging activity of the H2L Schiff base ligand and its the Cu(II) coordination polymer was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interaction and radical activity studies, the Cu(II) coordination polymer was found to give better results than free the H2L Schiff base ligand.
A potent drug candidature of Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes with bioactive ligands: synthesis, crystal structures, biomolecular interactions, radical scavenging and cytotoxicities
(Taylor and Francis, 2020-08-01) İnci, Duygu; Zorlu, Yunus; Aydın, Rahmiye; Vatan, Özgür; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-7687-3284; 0000-0003-4944-0181; O-7508-2015; AAH-8936-2021; 56261495600; 16235098100
A novel ternary copper(II) complexes, - [Cu(py-phen)(asn)(NO3)(H2O)] (1) and [Cu(py-phen)(trp)(H2O)]NO3(2)- (py-phen: pyrazino[2,3-f][1,10]phenanthroline, asn: asparagine, trp: tryptophan), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complexes1and2with CT-DNA has been investigated by absorption spectral titration, EB and Hoechst 33258 displacement assay. The interaction between the complexes1and2and BSA was investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes indicate that the fluorescence quenching mechanism between the complexes1and2and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (Delta G, Delta H, Delta S) of BSA + complex systems were determined at different temperatures. The binding distance between the complexes1and2and BSA was calculated according to FRET. The effect of the complexes1and2on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complex was determined in terms of EC50, using the DPPH and H(2)O(2)method. The anticancer activities of the complexes1and2were investigated using an XTT assay against three cancer cell lines (MCF-7, Caco-2 and A549) and non-tumor cell line (BEAS-2B). A potent drug candidature of two new copper(II) complexes, - [Cu(py-phen)(asn)(NO3)(H2O)] (1) and [Cu(py-phen)(trp)(H2O)]NO3(2)- (py-phen: pyrazino[2,3-f][1,10]phenanthroline, asn: asparagine, trp: tryptophan), have been synthesized and characterized by CHN analysis, FTIR, ESI-MS and single-crystal X-ray diffraction techniques. The complexes have been tested for theirin vitrobiomolecular interactions by the spectroscopic methods. Furthermore, radical scavenging and anticancer activities of the complexes was also investigated.
Synthesis and crystal structures of novel copper(II) complexes with glycine and substituted phenanthrolines: reactivity towards DNA/BSA and in vitro cytotoxic and antimicrobial evaluation
(Springer, 2017-01) Zorlu, Yunus; Yerli, Yusuf; Coşut, Bünyemin; İnci, Duygu; Aydın, Rahmiye; Vatan, Özgür; Sevgi, Tuba; Yılmaz, Dilek; Demirkan, Elif; Cinkılıç, Nilüfer; Uludağ Üniversitesi/Fen-Edebiyet Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-0483-9642; 0000-0002-7687-3284; 0000-0002-7528-9529; 0000-0002-3595-6286; G-2201-2019; AAH-8936-2021; O-7508-2015; AAG-7112-2021; ABI-4472-2020; AAH-5296-2021; 55082306300; 56261495600; 16235098100; 57191880859; 6701369462; 23469245200; 26533892300
New copper(II) complexes-dimeric-[Cu(nphen)(gly)(H2O)](+) (1) and [Cu(dmphen)(gly)(NO3)(H2O)] (2) (nphen = 5-nitro-1,10-phenanthroline, dmphen = 4,7-dimethyl-1,10-phenanthroline, and gly = glycine)-have been synthesized and characterized by CHN analysis, single-crystal X-ray diffraction techniques, FTIR, EPR spectroscopy, and cyclic voltammetry. The CT-DNA-binding properties of these complexes have been investigated by thermal denaturation measurements and both absorption and emission spectroscopy. The DNA cleavage activity of these complexes has been studied on supercoiled pUC19 plasmid DNA by gel electrophoresis experiments in the absence and presence of H2O2. Furthermore, the interaction of these complexes with bovine serum albumin (BSA) has been investigated using absorption and emission spectroscopy. The thermodynamic parameters, free-energy change (Delta G), enthalpy change (Delta H), and entropy change (Delta S) for BSA + complexes 1 and 2 systems have been calculated by the van't Hoff equation at three different temperatures (293.2, 303.2, and 310.2 K). The distance between the BSA and these complexes has been determined using fluorescence resonance energy transfer (FRET). Conformational changes of BSA have been observed using the synchronous fluorescence technique. In addition, in vitro cytotoxicities of these complexes on tumor cell lines (Caco-2, A549, and MCF-7) and healthy cells (BEAS-2B) have been examined. The antimicrobial activity of the complexes has also been tested on certain bacteria cells. The effect of mono and dimeric in the above complexes is presented and discussed. New copper(II) complexes-dimeric-[Cu(nphen)(gly)(H2O)](+) (1) and [Cu(dmphen)(gly) (NO3)(H2O)] (2) (nphen = 5-nitro-1,10-phenanthroline, dmphen = 4,7-dimethyl-1,10-phenanthroline and gly = glycine)-have been synthesized and characterized by CHN analysis, single-crystal X-ray diffraction techniques, FTIR and EPR spectroscopy. They have been tested for their in vitro DNA/BSA interactions by the spectroscopic methods. These complexes exhibited higher cytotoxic and antimicrobial activities. Complex 1 shows better DNA / BSA interactions in comparison to complex 2.
Synthesis, crystal structure, stability studies, DNA/albumin interactions, and antimicrobial activities of two Cu(II) complexes with amino acids and 5-nitro-1,10-phenanthroline
(Taylor & Francis, 2016-11) Zorlu, Yunus; İnci, Duygu; Aydın, Rahmiye; Sevgi, Tuba; Demirkan, Elif; Uludağ Üniversitesi/Fen-Edebiyet Fakültesi/Kimya Bölümü.; Uludağ Üniversitesi/Fen-Edebiyet Fakültesi/Biyoloji Bölümü.; 0000-0002-0483-9642; 0000-0002-7528-9529; G-2201-2019; AAH-8936-2021; AAG-7112-2021; ABI-4472-2020; 55082306300; 56261495600; 57191880859; 23469245200
One-dimensional (1-D) coordination polymer and mononuclear copper(II) complexes, ([Cu(nphen)(asn)]ClO4)(n) (1) and [Cu(nphen) (gln)(H2O)]ClO4 center dot H2O (2) (nphen = 5-nitro-110-phenanthroline, asn = asparagine, gln = glutamine), have been synthesized and characterized by IR spectroscopy, ESI-MS, CHN analysis, and singlecrystal X-ray diffraction. These binary and ternary complexes of copper(II) with nphen, asn, and gln have been investigated using potentiometric methods in 0.1 MKCl aqueous ionic media at 298.2 K. The protonation constants of the ligands and the stability constants of 1 and 2 have been calculated from the potentiometric data using the "BEST" software package. The potentiometric results have been analyzed using the "SPE" software package, and the distribution curves for the copper-containing species have been determined for the ternary systems. The CT-DNA-binding properties of these complexes have been investigated by thermal denaturation measurements and both absorption and emission spectroscopy. Further, the interaction of these complexes with bovine serum albumin (BSA) and human serum albumin (HSA) has been investigated using absorption and emission spectroscopy. The thermodynamic parameters, free energy change (Delta G), enthalpy change (Delta H), and entropy change (Delta S) were calculated by the van't Hoff equation and discussed. The distances between the serum albumins and 1 and 2 have been obtained according to fluorescence resonance energy transfer (FRET). Conformational changes of serum albumins have been observed from synchronous fluorescence technique. The antimicrobial activity of the complexes has also been tested on some bacteria. The effect of different amino acids on the copper(II) complexes are discussed.