PubMed

Permanent URI for this collectionhttps://hdl.handle.net/11452/24864

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Environmental distribution of Cryptococcus neoformans and C. gattii around the Mediterranean basin
(Oxford University Press, 2016-06) Cogliati, Massimo; D'Amicis, Roberta; Zani, Alberto; Montagna, Maria Teresa; Caggiano, Giuseppina; De Giglio, Osvalda; Balbino, Stella; De Donno, Antonella; Serio, Francesca; Susever, Serdar; Ergin, Çağrı; Velegraki, Aristea; Ellabib, Mohamed S.; Nardoni, Simona; Macci, Cristina; Oliveri, Salvatore; Trovato, Laura; Dipineto, Ludovico; Rickerts, Volker; McCormick-Smith, Ilka; Mlinaric-Missoni, Emilija; Bertout, Sebastien; Mallie, Michele; Martins, Maria da Luz; Venca, Ana C. F.; Vieira, Maria L.; Sampaio, Ana C.; Pereira, Cheila; Griseo, Giuseppe; Romeo, Orazio; Ranque, Stephane; Al-Yasiri, Mohammed H. Y.; Kaya, Meltem; Çerikcioğlu, Nilgün; Marchese, Anna; Vezzulli, Luigi; Ilkit, Macit; Desnos-Ollivier, Marie; Pasquale, Vincenzo; Korem, Maya; Polacheck, Itzhack; Scopa, Antonio; Meyer, Wieland; Ferreira-Paim, Kennio; Hagen, Ferry; Theelen, Bart; Tortorano, Anna Maria; Dromer, Francoise; Varma, Ashok; Kwon-Chung, Kyung J.; Inacio, Joeao; Alonso, Beatriz; Colom, Maria F.; Akçağlar, Sevim; Töre, Okan; Uludağ Üniversitesi/Tıp Fakültesi.; 0000-0002-4759-9634; GCO-2901-2022; CHJ-4532-2022; 6506194958; 6505909596
In order to elucidate the distribution of Cryptococcus neoformans and C. gattii in the Mediterranean basin, an extensive environmental survey was carried out during 2012-2015. A total of 302 sites located in 12 countries were sampled, 6436 samples from 3765 trees were collected and 5% of trees were found to be colonized by cryptococcal yeasts. Cryptococcus neoformans was isolated from 177 trees and C. gattii from 13. Cryptococcus neoformans colonized 27% of Ceratonia, 10% of Olea, Platanus and Prunus trees and a lower percentage of other tree genera. The 13 C. gattii isolates were collected from five Eucalyptus, four Ceratonia, two Pinus and two Olea trees. Cryptococcus neoformans was distributed all around the Mediterranean basin, whereas C. gattii was isolated in Greece, Southern Italy and Spain, in agreement with previous findings from both clinical and environmental sources. Among C. neoformans isolates, VNI was the prevalent molecular type but VNII, VNIV and VNIII hybrid strains were also isolated. With the exception of a single VGIV isolate, all C. gattii isolates were VGI. The results confirmed the presence of both Cryptococcus species in the Mediterranean environment, and showed that both carob and olive trees represent an important niche for these yeasts.
Genotypes and population genetics of cryptococcus neoformans and cryptococcus gattii species complexes in Europe and the Mediterranean area
(Academic Press Inc Elsevier Science, 2019-04-03) Akçağlar, Sevim; Bursa Uludağ Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri/Tıbbi Mikrobiyoloji Anabilim Dalı.; 0000-0002-4759-9634; 6506194958
A total of 476 European isolates (310 Cryptococcus neoformans var. grubii, 150 C. neoformans var. neoformans, and 16 C. gattii species complex) from both clinical and environmental sources were analyzed by multi-locus sequence typing. Phylogenetic and population genetic analyses were performed. Sequence analysis identified 74 sequence types among C. neoformans var. neoformans (VNIV), 65 among C. neoformans var. grubii (56 VNI, 8 VNII, 1 VNB), and 5 among the C. gattii species complex (4 VGI and 1 VGIV) isolates. ST23 was the most frequent genotype (22%) among VNI isolates which were mostly grouped in a large clonal cluster including 50% of isolates. Among VNIV isolates, a predominant genotype was not identified. A high percentage of autochthonous STs were identified in both VNI (71%) and VNIV (96%) group of isolates. The 16 European C. gattii species complex isolates analyzed in the present study originated all from the environment and all belonged to a large cluster endemic in the Mediterranean area. Population genetic analysis confirmed that VNI group of isolates were characterized by low variability and clonal expansion while VNIV by a higher variability and a number of recombination events. However, when VNI and VNIV environmental isolates were compared, they showed a similar population structure with a high percentage of shared mutations and the absence of fixed mutations. Also linkage disequilibrium analysis reveals differences between clinical and environmental isolates showing a key role of PLB1 allele combinations in host infection as well as the key role of LAC1 allele combinations for survival of the fungus in the environment. The present study shows that genetic comparison of clinical and environmental isolates represents a first step to understand the genetic characteristics that cause the shift of some genotypes from a saprophytic to a parasitic life style.
Two cases of cryptococcal meningitis in immunocompromised patients not infected with HIV
(Wiley, 2007-01-18) Akçağlar, Sevim; Sevgican, Emine; Akalın, Halis; Ener, Beyza; Töre, Okan; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Anabilim Dalı.; 0000-0002-4803-8206; 0000-0002-4759-9634; AAU-8952-2020; AAG-8523-2021; 6506194958; 16246450600; 57207553671; 15053025300; 6505909596
We present the cases of two patients with cryptococcal meningitis who were predisposed to fungal infection because of diabetes mellitus (Case 1) and systemic lupus erythematosus (Case 2). Both patients were tested negative for anti-HIV antibodies.

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