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Browsing by BUU Author "Akçağlar, Sevim"

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    Acid proteinase enzyme activity in Candida albicans strains: A comparison of spectrophotometry and plate methods
    (TÜBİTAK, 2011) Akçağlar, Sevim; Ener, Beyza; Töre, Okan; Uludağ Üniversitesi/Tıp Fakültesi/Mikrobiyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı.; 0000-0002-4803-8206; AAG-8523-2021; 6506194958; 15053025300; 6505909596
    In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > 0.05).
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    Comparative evaluation of the antifungal susceptibility of Candida isolates from blood specimens: Results of a study in a tertiary care hospital in Bursa, Turkey
    (TÜBİTAK, 2010-02) Evci, Canan; Ener, Beyza; Göral, Güher; Akçağlar, Sevim; Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı.; 0000-0002-4803-8206; 0000-0002-4759-9634; AAG-8523-2021; 22034011200; 15053025300; 6603453166; 6506194958
    Aim: Antifungal susceptibility testing may be an important aid in the treatment of patients with life threatening yeast infections. The aim of this study was to determine the susceptibility of Candida isolates obtained from clinical specimens to the antifungal agents amphotericin B and fluconazole, which are frequently used in our clinical practice. Materials and methods: Susceptibility to antifungal agents was investigated using the Etest (AB Biodisk, Solna, Sweden) and the reference broth macrodilution susceptibility test (CLSI M27-A2). The MICs obtained with each of the methods were read after 24 h and 48 h of incubation. Results: Overall agreement rates within +/- 1 dilution between the 2 methods were 86.7% (24 h) and 94.1% (48 h) for amphotericin B, and 85.5% (24 h) and 73.3% (48 h) for fluconazole. All C. albicans, C. pelliculosa, C. glabrata, and C. kefyr isolates were inhibited by <= 1 mu g mL-1 of amphotericin B, and fluconazole resistance was not observed in C. albicans or C. tropicalis isolates according to the 2 methods. Conclusion: According to the results of this study, decreased susceptibility to fluconazole and amphotericin B was most prominent in C. parapsilosis, C. krusei, and C. glabrata isolates.