2008 Cilt 2 Sayı 6
Permanent URI for this collectionhttps://hdl.handle.net/11452/16901
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Browsing by Department "Ziraat Fakültesi"
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Item Comparison of some characterizations of recovered from soil and newly fermented entomopathogenic nematode, heterorhabditis bacteriophora (rhabditida: heterorhabditidae)(Uludağ Üniversitesi, 2008) Ehlers, Ralf-Udo; Susurluk, I. Alper; Ziraat Fakültesi; Bitki Koruma BölümüEntomopathogenic nematodes (EPNs) in the families Heterorhabditidae and Steinernematidae have considerable potential as biological control agents of soil-inhabiting insect pests. One of the most important factors for sustainable biological control is their successful establishment in the soil, infectivity against pest insects, reproductive potential and persistence in released areas. To determine the differences of infectivity, reproductive potential and persistence between recovered from the field one year after release and newly fermented (one-week-old) Heterorhabditis bacteriophora in a bioreactor, the two type populations of H. bacteriophora were compared each other on these capabilities. Prior to study, the fermented nematodes were reproduced once in Galleria mellonella larvae in order to achieve equal conditions with recovered populations. The results showed that infectivity and reproduction potentials of recovered populations were significantly higher than newly fermented or laboratory populations of the nematode. However, differences of persistence of both populations were not significant. This is the first report that quality control of some biological abilities of H. bacteriophora after one year in field.Item Peroxidase activity and lipid peroxidation in strawberry (fragaria x ananassa) plants under low temperature(Uludağ Üniversitesi, 2008) Gülen, Hatice; Çetinkaya, Cem; Kadıoğlu, Murat; Kesici, Müge; Cansev, Asuman; Eriş, Atilla; Ziraat Fakültesi; Bahçe Bitkileri BölümüThe activity of peroxidase (PRX) isozyme, lipid peroxidation (Malondialdehyde, MDA content) and cell membrane injury were studied during low temperature treatment for different periods in strawberry (Fragaria x ananassa cv. Camarosa) leaf tissues. Seedlings were grown for six weeks (plants had 4-5 leaves) in a greenhouse then the plants were transferred to a climate chamber with constant 5o C, 60% relative humidity, 14/10 h (light/dark) photoperiod regime and 4 LS light intensity for 1, 4, 7 or 10 days to impose a low temperature stress. In general, low temperature application during 10 days caused a linear increase in MDA content. Native polyacrylamide gel electrophoresis (PAGE) of both acidic and basic peroxidase (PRX) isozymes yielded a single sharp protein band with Rf=0.23 and Rf=0.17, respectively. In addition data indicated a strong relationship between band intensities and the duration of the low temperature treatment. However, the considerable increase of PRX activities could not stop the deleterious effects of low temperature, but reduced severity of stress, thus showing a reduction in the percentage of injury on the 7th day which is correlated with cold-acclimation of strawberry leaf tissues under low temperature.