2016 Cilt 35 Sayı 1-2

Permanent URI for this collectionhttps://hdl.handle.net/11452/14437

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Browsing by Subject "Heat inactivation"

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    Bovine viral diarrhea virus propagates in cell culture after ınoculation of ınactivated sera from persistently ınfected calves
    (Uludağ Üniversitesi, 2016-12-12) Göktuna, Pelin Tuncer; Yeşilbağ, Kadir; Uludağ Üniversitesi/Veteriner Fakültesi.
    During routine diagnostic investigations on submitted serum and swab samples from three (n:3) calves in our laboratory for detecting BVDV antibody titers and antigen we noticed that successful virus propagation can occur from heat inactivated (in a water bath at 56° C for 30 min) 2 serum samples. For further confirmation of virus propagation in heat inactivated serum samples, 11 sera which were previously detected positive for BVDV were also investigated. As a result, from a total of 14 heat inactivated-field originated sera samples virus propagation was confirmed in 4 (28.5%) samples. This result suggests that heat inactivation of the serum is not eliminating the risk of pestivirus contamination, thus interfere the results of virus isolation and serological diagnostic methods including serum neutralization. Moreover relative resistance of BVDV to heat inactivation may lead to failure in complete inactivation of the virus in fetal calf sera which is an important contaminant for biologicals like cell cultures and attenuated live vaccines.