2005 Cilt 24 Sayı 1-2-3-4

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Influence of acute serious haemorrhagic shock on erythrocyte glutathione and sodium – potassium contents
(Uludağ Üniversitesi, 2005-03-27) Yalçın, Murat; Galip, Nurten; Uludağ Üniversitesi/Veteriner Fakültesi.
The aim of the present study is to describe erythrocyte glutathione level and sodium – potassium contents at acute serious haemorrhage. The experiment was performed on adult male 15 Sprague-Dawley rats. Haemorrhage was performed by withdrawing a total volume of 2.1 ml of blood / 100 g body weight over a period of 10 min. Blood was collected twice; before haemorrhage and 1 hour after haemorrhage. Haemorrhage caused approximately 20 % decrease in hematocrit, red blood cells count, amount of haemoglobin and about 60 % decrease in mean arterial pressure but 46 % increase in heart rate. Interestingly, erythrocyte glutathione value does not change in pre- and post-haemorrhage. Moreover, haemorrhage increased plasma and erythrocyte sodium values but decreased plasma and erythrocyte potassium values. In conclusion, due to hemorrhagic shock, increased production of reactive oxygen species is a feature of haemorrhagic shock and decreased erythrocytes by haemorrhage can respond to oxidative stress by upregulating antioxidant defence in terms of increased production of glutathione. Moreover, the observed high sodium concentrations associated with a potassium decrease in red blood cell can be defect in cellular function due to severe hemorrhagic shock.