Person: SAĞIRKAYA, HAKAN
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SAĞIRKAYA
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HAKAN
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Publication L-Carnitine supplemented extendersimprove post-thawing quality of honey bee drone (Apis mellifera) Spermatozoa [1](Kafkas Üniversitesi, 2021-07-01) Alçay, Selim; Çakmak, Selvinar; Çakmak, İbrahim; Aktar, Ahmet; Yılmaz, Melih; Üstüner, Burcu; Akkaşoğlu, Mustafa; Taşkıran, Şeyma; Ayaz, Elif; Sağırkaya, Hakan; Nur, Zekariya; ALÇAY, SELİM; Çakmak, Selvinar; ÇAKMAK, İBRAHİM; Aktar, Ahmet; Yılmaz, Melih; ÜSTÜNER, BURCU; Akkaşoğlu, Mustafa; Taşkıran, Şeyma; Ayaz, Elif; SAĞIRKAYA, HAKAN; NUR, ZEKARİYA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Döreme ve Suni Tohumlama Anabilim Dalı.; Bursa Uludağ Üniversitesi/Arıcılığı Geliştirme-Uygulama ve Araştırma Merkezi.; 0000-0002-8000-5770; 0000-0002-2975-2594; 0000-0002-1438-221X; CBC-7350-2022; ENZ-0848-2022; LMK-3522-2024; CBC-1744-2022; HSP-0413-2023; AAG-7238-2021; EMD-6376-2022; EAY-3244-2022; IBA-3364-2023; DPO-0081-2022; AAH-2635-2021The study aimed to evaluate the effects of L-carnitine on honey bee (Apis mellifera) drone spermatozoon at post-thaw. Semen samples obtained from sexually mature drones were pooled. Then, pooled semen was diluted with different concentrations of L-carnitine (2.5 mM/5 mM/10 mM) and without L-carnitine (control) supplemented extenders. Motility, plasma membrane functional integrity, acrosomal integrity and mitochondrial function were negatively affected by the cryopreservation process but DNA integrity was not affected. Malondialdehyde (MDA) concentration was used for analyzing the membrane lipid peroxidation status and a better result was obtained in the LC5 group compared with the control group at post-thaw. L-carnitine groups had a positive effect on sperm motility, plasma membrane integrity, and mitochondrial function compared to the control group (P<0.05). Acrosomal integrity was better preserved in the LC5 group compared to the control group. The study shows that LC supplemented extenders have beneficial effects on honey bee drone sperm parameters. The results of the present study demonstrated the beneficial effect of using a 5 mMol LC supplemented extender.Publication Autologous platelet rich plasma have positive effect on ram spermatozoa during cryopreservation in non-breeding season(Kafkas Üniversitesi, 2022-02-07) Alçay, Selim; Aktar, Ahmet; Koca, Davut; Kılıç, Mehmet Ali; Akkaşoğlu, Mustafa; Yılmaz, Mehmet Melih; Sağırkaya, Hakan; ALÇAY, SELİM; Aktar, Ahmet; KOCA, DAVUT; Kılıç, Mehmet Ali; Akkaşoğlu, Mustafa; Yılmaz, Mehmet Melih; SAĞIRKAYA, HAKAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Döllenme ve Suni Tohumlama Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Jinekoloji Anabilim Dalı.; 0000-0002-2975-2594; 0000-0002-7962-6959; 0000-0003-4482-3490; 0000-0001-6050-791X; C-7150-2016; GWC-2055-2022; AAI-6709-2021; CBC-7350-2022; CBC-1744-2022; EMD-6376-2022; DPO-0081-2022The aim of this study was to evaluate the effects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen after thawing. Semen samples were collected from nine Kivircik rams and each semen was divided into four equal aliquots and mixed with different concentrations of PRP supported extenders [no PRP (control), 12.5x10(6)/mL PRP, 25x10(6)/mL PRP, or 50x10(6)/mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed after thawing. The results showed that 25x10(6)/mL PRP group had positive effect on motility (61.67 +/- 3.81), membrane functional integrity (71.00 +/- 2.96) and MDA levels (5.13 +/- 0.64) at post-thawed (P<0.05). It was determined that 25x10(6)/mL PRP and 50x10(6)/mL PRP groups were more effective than other groups in terms of mitochondrial membrane potential (69.50 +/- 1.93), acrosome integrity (78.04 +/- 2.65) and DNA integrity (5.33 +/- 0.92). The results of the study showed that autologous PRP has a protective effect in the cryopreservation of ram spermatozoa.Publication Positive effect of autologous platelet rich plasma on saanen buck semen cryopreservation in non-breeding season(Academic Press Inc Elsevier Science, 2021-12-01) Alçay, Selim; Aktar, Ahmet; Koca, Davut; Kılıç, Mehmet Ali; Akkaşoğlu, Mustafa; Sağırkaya, Hakan; ALÇAY, SELİM; Aktar, Ahmet; KOCA, DAVUT; Kılıç, M. Ali; Akkaşoğlu, Mustafa; SAĞIRKAYA, HAKAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Doğum ve Jinekoloji Bölümü; 0000-0002-2975-2594; 0000-0002-7962-6959; 0000-0003-4482-3490; AAI-6709-2021; C-7150-2016; CBC-7350-2022; CBC-1744-2022; EMD-6376-2022; DPO-0081-2022The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5 x 107/ml PRP, 1 x 107/ml PRP, or 2 x 107/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2 x 107/ml PRP group had a positive effect on motility (62.41 +/- 4.24), membrane functional integrity (71.11 +/- 2.90), mitochondrial membrane potential (69.70 +/- 1.99), DNA integrity (7.22 +/- 0.93) and MDA levels (2.56 +/- 0.73) at post-thaw (P < 0.05). The results of the study demonstrated that autologous PRP has a protective effect on cryopreservation of buck spermatozoa and the fertility effects are worthy of further study.Publication Production of chimeric cattle embryos by reaggregation of blastomeres obtained from day 4 bovine embryos(Tubitak Scientific & Technological Research Council Turkey, 2004-01-01) Sağırkaya, Hakan; SAĞIRKAYA, HAKAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi.; AAH-8821-2021The aim of this study was to investigate the possibility of producing chimeric bovine embryos by reaggregating blastomeres of disaggregated day 4 bovine embryos. Oocytes were obtained from a slaughterhouse. Selected oocytes were matured, fertilized and cultured in vitro. Fertilization day was taken as day 0. Blastomeres of day 4 embryos were disaggregated and then pooled together. Chimeric embryos were randomly constructed by reaggregating blastomeres in empty zonae pellucidae. Non-chimeric handled control embryos were produced by transferring blastomeres from each embryo into their own empty zonae pellucidae. A total of 79 day 4 cattle embryos were used for disaggregation of blastomeres, and 27 chimeric and 12 non-chimeric handled control embryos were obtained from them. The rates of blastocyst formation of chimeric and non-chimeric handled control embryos were 40.7% (11/27) and 50.0% (6/12). respectively, and the difference between them was not statistically important (P > 0.05). These results showed the possibility of producing chimeric cattle embryos by reaggregating blastomeres obtained from day 4 embryos.Publication Effect of embryo quality on pregnancy outcome in recipient cows and heifers(Hellenic Veterinary Medical Soc, 2020-04-01) Kara, Uğur; Çoban, S.; Say, Erkan; Hızlı, Hatice; Mutlu, H.; Sagırkaya, H.; Ayasan, T.; Coskun, M. A.; Asarkaya, A.; Erten, H. E.; Yilmaz, I.; Yazgan, E.; Erdoğan, I. E.; Erten, Z. M.; Ergül, S.; Sert, F.; Nak, Yavuz; NAK, YAVUZ; Sağırkaya, Hakan; SAĞIRKAYA, HAKAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Doğum ve Jinekoloji Anabilim Dalı.; AAH-5494-2021; AAH-8821-2021This study was performed to compare the effects of embryo quality on pregnancy outcome in recipient cows and heifers. Embryos obtained from 83 Holstein donors were used in the study. In order to synchronize the recipients, 500 mu g of cloprostenol were administered intramuscularly (i.m.), twice, at an interval of 11 days. In all recipients, one embryo was transferred to the upper 1/3 of the uterine horn ipsilateral to the ovary with the corpus luteum on the 7th day after oestrus onset (day 0). Each recipient received an i.m. injection of 5 mu g of buserelin at the time ofembryo transfer. Pregnancy examinations were performed by ultrasonography on the day 30th post-transfer. A total of 345 (262 grade 1, 64 grade 2 and 19 grade 3) fresh embryos were transferred to 171 recipient cows and 174 recipient heifers. The pregnancy rates of the recipient cows and heifers were 41.52% and 52.29%, respectively (p<0.05).The pregnancy rates achieved with first, second and third quality grade embryos were 45.16%, 25.0% and 12.5% in the cows (p<0.05), and 55.11%, 41.66% and 54.54% in the heifers (p>0.05), respectively. In conclusion, the pregnancy rates was significantly higher in heifers than in cows (p<0.05) and, the embryo quality had a significant impact on recipient pregnancy outcome (p<0.05). The effect of embryo quality on pregnancy was significant in cows (p<0.05), but not significant in heifers (p>0.05).Publication Searching of pregnancy rate in repeat breeder cows by embryo transfer practices(Galenos Publ House, 2021-01-01) Say, Erkan; Sağırkaya, Hakan; SAĞIRKAYA, HAKAN; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Döllenme ve Suni Tohumlama Anabilim Dalı; DPO-0081-2022The aim of this study is to compare the pregnancy rates by applying embryo transfer to the cows which are not pregnant and should be removed from herd as repeat breeder. In this study, 87 randomly selected Holstein cows utilized. The repeat breeder cows (n=45) were selected from at least one giving birth, having regular sexual cycle, missing clinical worsening into genital organ and not displaying an abnormal discharge. On the other hand, it was selected from nonpregnant cows which inseminated artificially at least 3 times or more. Besides, cows that used as the control group (n=42) were selected from the cows without any artificial insemination postnatally. The PGF2 alpha application was performed to all recipient cows which are considered to benefit from as a recipient in control and testing groups just 24 days before the flushing day. After this application, the cows showing estrous symptoms were recorded and determined as candidate recipients. The pregnancy rates were 35.6% and 50% for testing and control groups, respectively and the difference between the groups was significant (P<0,05). As a result, even this difference between the groups it has been concluded that embryo transfer can be used to conceive especially for high-yielding cows as a treatment method for repeat breeder cows. Thereby, embryo transfer from the cows with high superior characteristics to the high milk yielding cows which have infertility problems can economically be beneficial by utilizing the high milk yield in the later lactation without any replacement cost.