Person: ARI, FERDA
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
ARI
First Name
FERDA
Name
20 results
Search Results
Now showing 1 - 10 of 20
Publication Toxicity assessment of hypericum olympicum subsp. olympicum l. on human lymphocytes and breast cancer cell lines(Univ South Bohemia, 2020-01-01) Balıkçı, Necmiye; Sarımahmut, Mehmet; Arı, Ferda; Aztopal, Nazlıhan; Özel, Mustafa Zafer; Ulukaya, Engin; Çelikler, Serap; Balıkçı, Necmiye; SARIMAHMUT, MEHMET; ARI, FERDA; Aztopal, Nazlıhan; Ulukaya, Engin; ÇELİKLER KASIMOĞULLARI, SERAP; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü; 0000-0003-2647-5875; 0000-0002-6729-7908; 0000-0003-3118-8061; 0000-0003-4875-5472; 0000-0002-4177-3478; JCN-7113-2023; AAG-7012-2021; AAV-4886-2020; K-5792-2018; L-6687-2018; JCD-5015-2023; ENX-2092-2022There is a limited number of studies about the constituents of Hypericum olympicum subsp. olympicum and its genotoxic and cytotoxic potency. We examined the possible antigenotoxic/genotoxic properties of methanolic extract of H. olympicum subsp. olympicum (HOE) on human lymphocytes by employing sister chromatid exchange, micronucleus and comet assay and analyzed its chemical composition by GCxGC-TOF/MS. The anti-growth activity against MCF-7 and MDA-MB-231 cell lines was assessed by using the ATP viability assay. Cell death mode was investigated with fluorescence staining and ELISA assays. The major components of the flower and trunk were determined as eicosane, heptacosane, 2-propen-1-ol, hexahydrofarnesyl acetone and alpha-muurolene. HOE caused significant DNA damage at selected doses (250-750 mu g/ml) while chromosomal damage was observed at higher concentrations (500 and 750 mu g/ml). HOE demonstrated anti-growth activity in a dose-dependent manner between 3.13-100 mu g/ml. Pyknotic nuclei were observed at 100 mu g/ml concentration of HOE in both cell lines. In conclusion, HOE demonstrated cytotoxic effects in a cell type-dependent manner, however its genotoxic effects were observed at relatively higher doses.Publication Design, synthesis and anticancer activity of new benzofuran-chalcone hybrids and their water soluble sodium salts(Wiley-v C H Verlag Gmbh, 2023-03-06) Coskun, Demet; Coşkun, Mehmet Fatih; Çınar-Asa, Sibel; Akgün, Oğuzhan; Akgün, Halime; Arı, Ferda; ARI, FERDA; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-8410-1786; 0000-0002-2048-3252; 0000-0002-6729-7908; A-5608-2019; ADX-9980-2022; AAG-7012-2021In this study, firstly, 1-(7-ethoxy-1-benzofuran-2-yl) ethanone and 1,1'-(7-ethoxy-1-benzofuran-2,4-diyl)diethanone were synthesized for the starting reagent purposes. The synthesized benzofuran-chalcone salts were soluble in water at room temperature. Structural analysis of the synthesized compounds was characterized by elemental analysis, FT-IR and NMR spectroscopy techniques. The anticancer activities of the compounds were determined by SRB viability assay in human lung cancer (A549, H1299) and breast cancer (MCF-7, MDA-MB-231) cell lines. Findings for apoptosis were determined by flow cytometry analysis and the PARP-ELISA method. The results of the in vitro SRB analysis of the compounds showed that some of the chalcone hybrids were very effective on both types of cancer in a dose and time-dependent manner. Treatment of all cancer cell types with these hybrids resulted in a significant increase in the percentage of early and mainly late apoptotic cells, demonstrating their apoptosis-inducing effects via the Caspase 3/7 Activity.Publication Targeting the epithelial-mesenchymal transition (emt) pathway with combination of wnt inhibitor and chalcone complexes in lung cancer cells(Wiley, 2023-07-14) Coşkun, Demet; Arı, Ferda; ARI, FERDA; Ertürk, Elif; ERTÜRK, ELİF; Onur, Ömer E.; Akgün, Oğuzhan; Aydın, İpek; İPEK, AYDIN; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; Bursa Uludağ Üniversitesi/Tıbbi Labaratuvar Teknikleri .; 0000-0002-8410-1786; 0000-0002-6729-7908; A-5608-2019; JQI-3400-2023; AAG-7012-2021; IUO-8513-2023Non-small cell lung cancer (NSCLC) is the most common type of the lung cancer. Despite development in treatment options in NSCLC, the overall survival ratios is still poor due to epithelial and mesenchymal transition (EMT) feature and associated metastasis event. Thereby there is a need to develop strategy to increase antitumor response against the NSCLC cells by targeting EMT pathway with combination drugs. Niclosamide and chalcone complexes are both affect cancer cell signaling pathways and therefore inhibit the EMT pathway. In this study, it was aimed to increase antitumor response and suppress EMT pathway in NSCLC cells by combining niclosamide and chalcone complexes. SRB cell viability assay was performed to investigate the anticancer activity of drugs. The drugs were tested on both NSCLC cells (A549 and H1299) and normal lung bronchial cells (BEAS-2B). Then the two drugs were combined and their effects on cancer cells were evaluated. Fluorescence imaging and enzyme-linked immunosorbent assay were performed on treated cells to observe the cell death manner. Wound healing assay, real-time quantitative polymerase chain reaction, and western blot analysis were performed to measure EMT pathway activity. Our results showed that niclosamide and chalcone complexes combination kill cancer cells more than normal lung bronchial cells. Compared to single drug administration, the combination of both drugs killed NSCLC cells more effectively by increasing apoptotic activity. In addition, the combination of niclosamide and chalcone complexes decreased multidrug resistance and EMT activity by lowering their gene expressions and protein levels. These results showed that niclosamide and chalcone complexes combination could be a new drug combination for the treatment of NSCLC.Publication Soloxolone methyl, as a 18βH-glycyrrhetinic acid derivate, may result in endoplasmic reticulum stress to induce apoptosis in breast cancer cells(Elsevier, 2021-01-15) Alper, Pınar; Salomatina, Oksana, V; Salakhutdinov, Nariman F.; Ulukaya, Engin; Arı, Ferda; Alper, Pınar; ARI, FERDA; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü; 0000-0001-9631-3551; 0000-0002-6729-7908; DUL-1586-2022; AAG-7012-2021Being one of the leading causes of cancer death among women, various chemotherapeutic agents isolated from natural compounds are used in breast cancer treatment and consequently studies to develop new drugs still continue. There are several studies on 18 beta H-glycyrrhetinic acid, a secondary metabolite which is found in Glycyrrhiza glabra (liquorice roots), as a potential anticancer agent. In this study, the cytotoxic and apoptotic effects of Soloxolone methyl compound, a semisynthetic derivative of 18 beta H-glycyrrhetinic acid were investigated on breast cancer cells (MCF-7, MDA-MBA-231). Soloxolone methyl is found to be cytotoxic on both MCF-7 and MDA-MBA-231 breast cancer cells by inducing apoptosis. Especially in MDA-MB-231 cells apoptosis is detected to be triggered by ER stress. The antigrowth effects of Soloxolone methyl were determined using MTT and ATP assays. To identify the mode of cell death (apoptosis/necrosis), fluorescent staining (Hoechst 33342 and Propidium iodide) and caspase-cleaved cytokeratin 18 (M30-antigen) analyses were used. In addition, apoptosis was investigated on gene and protein levels by PCR and Western Blotting. Soloxolone methyl decreased cell viability on cells in a dose and time-dependent manner and induced apoptosis markers. An increase on apoptotic proteins related to endoplasmic reticulum stress (IRE1-alpha, Bip, CHOP) was also determined in MDA-MB-231 cells. Moreover, an increase of apoptotic gene expressions was determined in both cells treated with Soloxolone methyl. Advance analyses should be performed to elucidate the potential of Soloxolone methyl as an anticancer agent in breast cancer treatment.Publication Epigenetic modulators combination with chemotherapy in breast cancer cells(Wiley, 2021-02-20) Arı, Ferda; Napieralski, Rudolf; Akgün, Oğuzhan; Magdolen, Viktor; Ulukaya, Engin; ARI, FERDA; Akgün, Oğuzhan; Bursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü; 0000-0002-6729-7908; 0000-0002-8410-1786; A-5608-2019; AAG-7012-2021Despite the concerning adverse effects on tumour development, epigenetic drugs are very promising in cancer treatment. The aim of this study was to compare the differential effects of standard chemotherapy regimens (FEC: 5-fluorouracil plus epirubicine plus cyclophosphamide) in combination with epigenetic modulators (decitabine, valproic acid): (a) on gene methylation levels of selected tumour biomarkers (LINE-1, uPA, PAI-1, DAPK); (b) their expression status (uPA and PAI-1); (c) differentiation status (5meC and H3K27me3). Furthermore, cell survival as well as changes concerning the invasion capacity were monitored in cell culture models of breast cancer (MCF-7, MDA-MB-231). A significant overall decrease of cell survival was observed in the FEC-containing combination therapies for both cell lines. Methylation results showed a general tendency towards increased demethylation of the uPA and PAI-1 gene promoters for the MCF-7 cells, as well as the proapoptotic DAPK gene in the treatment regimens for both cell lines. The uPA and PAI-1 antigen levels were mainly increased in the supernatant of FEC-only treated MDA-MB-231 cells. DAC-only treatment induced an increase of secreted uPA protein in MCF-7 cell culture, while most of the VPA-containing regimens also induced uPA and PAI-1 expression in MCF-7 cell fractions. Epigenetically active substances can also induce a re-differentiation in tumour cells, as shown by 5meC, H3K27me3 applying ICC.Significance of the study Epigenetic modulators especially in the highly undifferentiated and highly malignant MDA-MB-231 tumour cells significantly reduced tumour malignancy thus; further clinical studies applying specific combination therapies with epigenetic modulators may be warranted.Publication Palladium (II) complex enhances ROS-dependent apoptotic effects via autophagy inhibition and disruption of multiple signaling pathways in colorectal cancer cells(Bentham Science Publ Ltd, 2021-01-01) Aydınlık, Şeyma; Erkısa, Merve; Arı, Ferda; Çelikler, Serap; Ulukaya, Engin; Aydınlık, Şeyma; ARI, FERDA; ÇELİKLER KASIMOĞULLARI, SERAP; 0000-0001-5238-2432; 0000-0002-6729-7908; 0000-0002-4177-3478; ABI-2909-2020; AAG-7012-2021; JCD-5015-2023Background: Inhibition of autophagy is reported to be a therapeutically effective strategy in overcoming resistance that is a deadly outcome in cancer. One of the most common reasons for chemo-resistance to treatment is the patients with tumors exhibiting a KRAS mutation, which occurs in approximately 40% of colorectal cancer patients.Objective: Hence, we assessed whether a Palladium (Pd)(II) complex is a promising anticancer complex, compared to 5-fluorouracil in KRAS wt HT-29 and KRAS mutant HCT-15 cells.Methods: HCT-15 and HT-29 cells were used for colorectal cancer and Chloroquine (CQ) was used as an inhibitor of autophagy. In this context, cells were treated with Pd(II) complex and 5-FU in combination with CQ for 48h and cell viability was measured by SRB assay. Cell death mode was examined with M30 and M65 ELISA assays, using annexin V/propidium iodide. Autophagy was determined by Acridine Orange (AO) staining. Furthermore, the expressions of various autophagy and apoptosis-related proteins were evaluated with Western blotting. Luminex assay and the level of Reactive Oxygen Species (ROS) were examined.Results: Cell viability was found to decrease in a dose-dependent manner and CQ enhanced cytotoxic effect in Pd(II) and 5-FU treated cells in colorectal cancer cells. Our data showed that inhibition of autophagic flux significantly increased intrinsic apoptosis through the activation of ROS. We showed that combinatorial treatment with CQ induced apoptosis via the caspase-dependent mitochondrial pathway. Luminex analysis revealed that the combination resulted in a down-regulation of NF-?B/AKT/CREB signaling pathways in both cell lines, however, decreased Erk1/2 protein expression was only observed after treatment with CQ combination in HCT-15 cells.Conclusion: We suggest that the inhibition of autophagy along with Pd(II) and 5-FU treatment has a synergistic effect on KRAS-mutant colorectal cancer cells. Autophagy inhibition by CQ promotes apoptosis via blockade of the NF-?B/AKT/CREB and activation of ROS.Publication Angelica sylvestris and delphinium staphisagria extracts induces antiproliferation through caspasemediated apoptosis on human cancer cells(Inst Tecnologia Parana, 2022-01-01) Şahin, Çağatay; ÇELİKLER KASIMOĞULLARI, SERAP; Akgün, Oğuzhan; Akgün, Halime; Çelikler, Serap; ARI, FERDA; Arı, Ferda; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0002-8410-1786; 0000-0002-2048-3252; 0000-0002-4177-3478; 0000-0002-6729-7908; AAG-7012-2021; A-5608-2019; JCD-5015-2023Angelica sylvestris and Delphinium staphisagria are medicinal and aromatic herbs with a long history in medicine and food industry. In this study, we have investigated anti-cancer activity of Angelica sylvestris and Delphinium staphisagria extracts on various cell lines of lung (A549), breast (MCF-7), colon (HT-29), and cervix (HeLa) origin. Also, cytotoxicity was tested on human healthy bronchial epithelial (BEAS-2B) cells. In vitro experiments showed that plant extracts suppressed cell growth and proliferation at low concentrations by reducing cell viability on cancer cells in a time and concentration-dependent manner. It was observed that Angelica sylvestris was more effective in HT-29 and HeLa cells and Delphinium staphisagria in A549 and MCF-7 cells by suppressing cell proliferation and increasing cell death. Cell death mode (apoptosis/necrosis) was investigated via fluorescent imaging, caspase-cleaved cytokeratin 18, activated caspase-3, and cleaved-PARP (poly (ADP-ribose) polymerase). In order to evaluate the cell death mode by plant extracts apoptotic markers were investigated by fluorescence staining. Delphinium staphisagria extract (50-200 mu g/mL) caused a decrease in cell density in A549 and MCF-7 cells compared to untreated controls. A similar situation was observed in HT-29 and HeLa cell lines when treated with ASE. As a result, Delphinium staphisagria extracts induced apoptosis in A549 and MCF-7, while Angelica sylvestris extracts induced apoptosis in HT-29 and HeLa cancer cells.Publication Investigation of anti-cancer activity of newly synthesized 2,4-pentadien-1-one derivative containing benzofuran in human lung and colon cancer cells(Kare Publ, 2023-01-01) Coşkun, Demet; Arı, Ferda; ARI, FERDA; Tuna, Gonca; Ertürk, Elif; ERTÜRK, ELİF; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-7141-6909; 0000-0002-6729-7908; AAG-7012-2021; IWM-5784-2023Objectives: A member of the flavonoid family, chalcones are natural compounds known to have anticancer effects. Chalcones and their synthetic derivatives have become an important field of interest for cancer research. In this study, we aimed to investigate the anticancer activity of a new Chalcone derivative compound [(2E,4E)-1-(7-ethoxy-1-benzofuran-2-yl)-5-(4-hydroxy-3-methoxyphenyl)penta-2,4-dien-1-one] synthesized by the Claisen-Schmidt reaction based on the curcumin structure in human lung (A549, H1299) and colon cancer (HCT116, HT29) cells. Methods: The effect of Chalcone compound on cell viability was evaluated with the SRB test. In addition, combination studies with 5-FU, which is used as a chemotherapy drug, was performed. The cell death mode was determined by fluorescence imaging method with Hoechst 33342, Annexin-V-FITC and Propidium iodide (PI) triple staining. Results: IC50 values of the Chalcone compound were found as 2.85, 1.46, 0.59, 0.35 mu M for A549, H1299, HCT116, HT29, respectively. As a result of fluorescence imaging, pycnotic nuclei and chromatin condensation were observed in the cells in addition to positive staining with Annexin-V-FITC (green). Conclusion: The results showed that the newly synthesized Chalcone derivative compound has a significant cytotoxic effect on cancer cells and induce apoptosis.Publication Cytotoxic and genotoxic effects of an endemic plant of Turkey salvia kronenburgii on breast cancer cell lines(Wolters Kluwer Medknow Publications, 2019-07-01) Çebi, Ayşegül; Akgün, Egemen; Çelikler, Serap; Fırat, Mehmet; Özel, Mustafa Zafer; Ulukaya, Engin; Arı, Ferda; ÇELİKLER KASIMOĞULLARI, SERAP; ARI, FERDA; Uludağ Üniversite/Fen Bilimleri Fakültesi/Biyoloji Fakültesi; AAG-7012-2021; JCD-5015-2023Context: The natural products derived from plants are the important sources that can be used for breast cancer treatment. Salvia species and their derived products were recommended as potential antitumor substances.Aim: The potential cytotoxic and genotoxic effects of Salvia kronenburgii have been investigated on breast cancer cell lines, MCF-7 and MDA-MB-231.Materials and Methods: Determination of chemical compounds of S. kronenburgii was done using a gas chromatography coupled to time-of-flight mass spectrometry system and a dual-stage commercial thermal desorption injector. Growth inhibition of the S. kronenburgii was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and ATP viability assays. The cell death mode was detected by fluorescent dyes. Genotoxic effect of S. kronenburgii was measured by comet assay.Results: S. kronenburgii showed antiproliferative effect in a dose-dependent manner on MCF-7 and MDA-MB-231 cell lines by inducing apoptosis-like cell death. The pyknotic cell nuclei were observed at the cell lines in response to S. kronenburgii. Furthermore, significant increase was shown in genetic damage index and frequencies in the damaged cells.Conclusion: S. kronenburgii might be a promising natural source for cancer therapy. Further experiments need to be done in vivo to understand of the anticancer effects of this plant.Publication Benzofuran substituted chalcone derivatives trigger apoptotic cell death through extrinsic pathway in human lung and breast cancer cells(Springer Int Publ Ag, 2023-07-12) Coşkun, Demet; Alioğlu, İmren; Çınar-Asa, Sibel; Arı, Ferda; ARI, FERDA; Bursa Uludağ Üniversitesi/Fen Edebiyat Fakültesi/Biyoloji Bölümü.; 0000-0001-7141-6909; 0000-0002-6729-7908; AAG-7012-2021; ADX-9980-2022In this study, the anticancer potential of benzofuran-substituted chalcone derivatives Compound 1 [(3-(Benzofuran-2-yl) -5-(4-N, N-dimethylaminophenyl)-2-pyrazoline)], Compound 2 [(4) -((1E)-3-(1)-benzofuran-2-yl)-3-oxoprop-1-en-1-yl]-2-methoxyphenylchloroacetate), Compound 3 [(3-(Benzofuran-2-yl)) -5-(thiophen-2-yl)-2-pyrazoline)] and Compound 4 [3-[(1E)-3-(1-benzofuran-2-yl)-3-oxoprop-1-en-1-yl)] phenyl chloroacetate) was investigated. Their cytotoxic and apoptotic effects were explored on the human breast (MCF-7 and MDA-MB-231) and lung (A549 and H1299) cancer cells by SRB and ATP cell viability assays and Hoechst 33342/Propidium Iodide dual staining. Expressions of proteins were examined by Western blot, and cell migration test was performed for metastatic effect. In conclusion, it has been shown that Compounds 2 and 4 have high cytotoxic activity on both human breast MCF-7 (IC50:9.37-2.71) and MDA-MB-231 (IC50:5.36-2.12) and lung A549 (IC50:3.23-2.21) and H1299 (IC50:6.07-2.92) cancer cell lines respectively. In addition, it has been found that Compounds 2 and 4 induce apoptosis via extrinsic pathway in the cells. Besides, they inhibited the migration of the cells showing antimetastatic potential.