Person: BUDAK, FERAH
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
BUDAK
First Name
FERAH
Name
43 results
Search Results
Now showing 1 - 10 of 43
Publication The effect of exosomes released from apheresis plateletconcentrates under the impact of gamma irradiation and storage time upon platelet aggregation and hemostasis(Simtipro Srl, 2023-05-01) Bal, Salih Haldun; Sağdilek, Engin; Karacay, Mehmet; Kızmaz, Muhammed A.; Kumaş, Levent T.; Can, Fatma E.; Yıldırım, Muzaffer; Canavar-Yıldırım, Tuğçe; Kosay-Gülkaya, Deniz; Yöyen-Ermiş, Diğdem; Budak, Ferah; Heper, Yasemin; Gürsel, İhsan; Oral, Haluk B.; BAL, SALİH HALDUN; SAĞDİLEK, ENGİN; Karaçay, Mehmet; Kizmaz, Muhammed A.; Kumaş, Levent T.; Can, Fatma E.; Kosay-Gülkaya, Deniz; YÖYEN ERMİŞ, DİĞDEM; BUDAK, FERAH; HEPER, YASEMİN; ORAL, HALUK BARBAROS; Tıp Fakültesi; Dr. Raşit Durusoy Kan Bankası; 0000-0001-7625-9148 ; 0000-0001-5871-8769 ; 0000-0001-5334-7911 ; 0000-0002-1953-7735; 0000-0002-1953-7735; HJY-9001-2023; AAH-4397-2021; JHB-7829-2023; HKN-2347-2023; FHB-1791-2022; JSL-7718-2023; HJH-5312-2023; HJH-5217-2023; IYU-6844-2023; AAH-3888-2021; IZP-9398-2023; HOK-0035-2023; K-7285-2012Background -Blood components should be gamma-irradiated (& gamma;-IR) in order to prevent transfusion-associated graft-versus-host disease. The aim of this study is to determine the effect of & Gamma;-IR and storage time on the exosomes released from apheresis platelet concentrates (aPC) and to investigate their impact on the maximum platelet aggregation (MPA) and hemostasis. Materials and methods -Eight units of aPC were included in this study. These were divided into four equal portions. Two portions were irradiated before storage while the other two were not. Thus, irradiated and non-irradiated aPC samples for storage Days 0 (D0) and 5 (D5) were obtained. Exosomes were isolated from these samples using a commercial kit and were evaluated to ascertain their parent cells by flow cytometry. For the following steps, exosomes were pooled according to their features. Pooled exosomes were then used for aggregometry and thromboelastography. Results -Platelet-derived exosome (PD-EX) levels decreased in D5 compared to D0 in NI-aPC, whereas granulocyte-derived exosome (GD-EX) levels increased. Exosome pools had no effect on MPA compared to saline groups. Exosome pools decreased the time to initial fibrin formation (R), whereas they increased the rate of clot formation (& alpha;-angle) and coagulation index (CI) compared to saline groups. Discussion -Storage time and & Gamma;-IR each have almost the opposite effects on PD-EX and GD-EX. Exosomes have no impact on MPA, but enhance the clot strength. The impact of exosomes on aPC quality and effectiveness can be ignored or considered as a positive effect.Publication The impact of gamma irradiation on exosome profiles and electrolyte levels in apheresis platelet concentrates(Wiley, 2021-08-01) Bal, Salih Haldun; Karaçay, Mehmet; Kızmaz, Muhammed Ali; Kumaş, Levent Tufan; Can, Fatma Ezgi; Gülkaya, Deniz Koşay; Ermiş, Diğdem Yöyen; Budak, Ferah; Heper, Yasemin; Oral, Haluk Barbaros; BAL, SALİH HALDUN; Karaçay, Mehmet; Kızmaz, Muhammed Ali; Kumaş, Levent Tufan; Can, Fatma Ezgi; Gülkaya, Deniz Koşay; YÖYEN ERMİŞ, DİĞDEM; BUDAK, FERAH; HEPER, YASEMİN; ORAL, HALUK BARBAROS; Tıp Fakültesi; İmmunoloji Ana Bilim Dalı; 0000-0001-5334-7911; 0000-0001-7625-9148; 0000-0003-0463-6818; JSL-7718-2023; HKN-2347-2023; K-7285-2012; F-4657-2014; IZP-9398-2023; KBR-5535-2024; JHB-7829-2023; FHB-1791-2022; CSL-9726-2022; GYL-2038-2022; HOK-0035-2023; CTY-9474-2022Publication The relationship between serum periostin levels, 6-minute walking test and quality of life in patients with idiopathic pulmonary fibrosis(Carbone Editore, 2019-01-01) Eken, Özge Aslantekin; ASLANTEKİN EKEN, ÖZGE; Coşkun, Funda; COŞKUN, NECMİYE FUNDA; Demirdöğen, Ezgi; DEMİRDÖĞEN, EZGİ; Dilektaşlı, Aslı Görek; GÖREK DİLEKTAŞLI, ASLI; Ursavaş, Ahmet; URSAVAŞ, AHMET; Budak, Ferah; BUDAK, FERAH; Karadağ, Mehmet; KARADAĞ, MEHMET; Tıp Fakültesi; İmmunoloji Ana Bilim Dalı; 0000-0003-3604-8826; 0000-0002-7400-9089; 0000-0001-7099-9647; 0000-0001-7625-9148; 0000-0002-9027-1132; AAG-8744-2021; AAI-3169-2021; JPK-7012-2023; AAD-1271-2019; IZP-9398-2023; F-4657-2014; AAH-9812-2021Introduction: Interstitial lung disease (ILD) is a group of chronic respiratory diseases characterized by inflammation and fi-brosis in different patterns at the interstitium, which is the most common form of idiopathic pulmonary fibrosis (IPF). Considering the prevalence, incidence, and outcomes of IPF, it is important to ensure the availability and validity of biomarkers that can be used in determining the diagnosis and prognosis. In this study, we aimed to assess the relationship between serum periostin levels, pulmonary function tests, 6-minute walking test (6MWT) and quality of life in IPF patients.Material and methods: Our study was carried out with 40 IPF patients who were followed at Uludag University, Faculty of Medicine, Department of Pulmonary Medicine. Spirometer and carbon monoxide diffusion test (DLCO) for respiratory functions, 6MWT and periostin level measurement were performed on the patients, and results were recorded.Results: A total of 31 of patients were males and nine were females. The diagnosis of IPF was made according to the guidelines of ATS / ERS / JRS / ALAT with 25 (62.5%) patients by radiologically and 15 (37.5%) patients with histopathological and radiological features. The mean periostin level was 32.9 +/- 37.57 ng/ml. No significant correlation was found between St. George's Respiratory Questionnaire (SGRQ) score, FVC% predicted, DLCO adj% predicted values, 6MWT distance and periostin level which were measured simultaneously. A negative correlation was found between SGRQ score and DLCO adj% predicted value, 6MWT distance.Conclusion: It has been shown that there is no correlation between periostin levels and the results of the pulmonary function tests, when measured and performed at the same time. No studies are evaluating the relationship between 6MWT and SGRQ scores and periostin levels. In our study, serum periostin level, pulmonary function test results, 6MWT and SGRQ scores were not significantly correlated.Publication Phenotypes and functions of low(er)-density neutrophils (LDNs) in early childhood and children(Wiley, 2021-08-01) Dombaz, Fatma; Karaçay, Mehmet; Etgü, Onur; Kızmaz, Muhammed Ali; Şimşek, Abdurrahman; Çağan, Eren; Pınar, İbrahim Ethem; Bal, Salih Haldun; Özkocaman, Vildan; Özkalemkaş, Fahir; Budak, Ferah; Oral, Halük Barbaros; Ermiş, Diğdem Yöyen; Dombaz, Fatma; Karaçay, Mehmet; Etgü, Onur; Kızmaz, Muhammed Ali; ŞİMŞEK, ABDURRAHMAN; PINAR, İBRAHİM ETHEM; BAL, SALİH HALDUN; ÖZKOCAMAN, VİLDAN; ÖZKALEMKAŞ, FAHİR; BUDAK, FERAH; ORAL, HALUK BARBAROS; YÖYEN ERMİŞ, DİĞDEM; Sağlık Bilimleri Yüksekokulu; İmmünoloji Ana Bilim Dalı; Hematoloji Bilim Dalı; 0000-0001-7625-9148 ; 0000-0001-9907-1498 ; 0000-0001-8850-0269 ; 0000-0001-5334-7911 ; DWR-5356-2022; JHB-7829-2023; JIJ-1849-2023; HKN-2347-2023; AAG-7381-2021; KBR-5535-2024; FQG-8981-2022; JIW-1248-2023; IZP-9398-2023; K-7285-2012; GYL-2038-2022Publication Serum cancer from lung-6: Promising biomarker to differentiate cpfe from ipf(Mattioli 1885, 2022-01-01) Uzaslan, Esra; DEMİRDÖĞEN, EZGİ; UZASLAN, AYŞE ESRA; GÖREK DİLEKTAŞLI, ASLI; ÖZKAYA, GÜVEN; Dilektaşlı, Aslı Görek; Öztürk, Nilüfer Aylin Acet; Karadağ, Mehmet; KARADAĞ, MEHMET; ACET ÖZTÜRK, NİLÜFER AYLİN; YEŞİLBURSA, DİLEK; Yeşilbursa, Dilek; Budak, Ferah; BUDAK, FERAH; Öztürk, Alper; Ursavaş, Ahmet; COŞKUN, NECMİYE FUNDA; URSAVAŞ, AHMET; Tıp Fakültesi; Kardiyoloji Ana Bilim Dalı; 0000-0002-7400-9089; 0000-0001-7099-9647; 0000-0002-6375-1472; 0000-0001-7625-9148; 0000-0003-3604-8826; 0000-0003-0297-846X; 0000-0002-9027-1132; AAD-1271-2019; JPK-7012-2023; IZP-9398-2023; A-4421-2016; F-4657-2014; AAI-3169-2021; AAG-8744-2021Background: Combined pulmonary fibrosis and emphysema (CPFE) has been recognised as a phe-notype of pulmonary fibrosis. We aimed to compare serum surfactant protein-A (SP-A), surfactant protein-D (SP-D) and Krebs von den Lungen-6 (KL-6) levels, functional parameters, in CPFE and IPF (idiopathic pul-monary fibrosis) patients. Methods: Patients diagnosed with ???CPFE??? and ???IPF??? were consecutively included in 6 months as two groups. The patients with connective tissue diseases are excluded. Results: In this study, 47 patients (41 males, 6 females) with CPFE (n = 21) and IPF (n = 26) with a mean age of 70.12 ?? 8.75 were evaluated. CPFE patients were older, had more intense smoking history, had lower DLCO/VA, lower FVC, and worse six-minute walking distance than the IPF group (p=0.005, p=0.027, p=0.02, p<0.001, p=0.001, respec-tively). Serum KL-6 levels were higher in CPFE group compared to IPF group [264.70 U/ml (228.90-786) vs 233.60 (101.8-425.4), p<0.001]. Serum KL-6 levels of 245.4 U/ml and higher have 81% sensitivity and 73% specificity for the discrimination of CPFE from IPF. Conclusions: Our study has shown that serum KL-6 level is a promising biomarker to differentiate CPFE from IPF. In CPFE cases respiratory and functional parameters are worse than those of pure fibrosis cases.Publication Evaluation of the roles of regulatory B (Breg) cells and B cell exhaustion in COVID-19(Wiley, 2021-08-01) Budak, Ferah; Çağan, Eren; Kızmaz, Muhammed Ali; Şimşek, Abdurrahman; Dombaz, Fatma; Tezcan, Gülçin; Asan, Ali; Bal, S. Haldun; Ermiş, Diğdem Yöyen; Demir, H. İbrahim; Ediger, Dane; Yılmaz, Emel; Oral, Haluk Barbaros; Akalın, E. Halis; BUDAK, FERAH; Kızmaz, Muhammed Ali; ŞİMŞEK, ABDURRAHMAN; Dombaz, Fatma; TEZCAN, GÜLÇİN; BAL, SALİH HALDUN; YÖYEN ERMİŞ, DİĞDEM; Demir, H. İbrahim; EDİGER, DANE; YILMAZ, EMEL; ORAL, HALUK BARBAROS; AKALIN, EMİN HALİS; Tıp Fakültesi; Temel Bilimler Bölümü; 0000-0001-7625-9148; 0000-0001-5334-7911; 0000-0001-8850-0269; 0000-0001-7288-3250; 0000-0002-5956-8755; 0000-0002-8856-7356; 0000-0001-7585-7971; 0000-0002-2954-4293; 0000-0003-1785-3539; 0000-0003-0463-6818; 0000-0001-7530-1279; AAG-7381-2021; AAH-3843-2020; K-7285-2012; F-4657-2014; IZP-9398-2023; AAU-8952-2020; HKN-2347-2023; DWR-5356-2022; KBR-5535-2024; GYL-2038-2022; GPN-1473-2022; AAE-9142-2019; GDP-0005-2022Publication Evaluation of indoleamine 2, 3 dioxygenase (IDO) gene polymorphisms in COVID-19(Wiley, 2021-08-01) Karaca, Mert; Arslan, Gözde; Ermiş, Diğdem Yöyen; Bal, Salih Haldun; Uzaslan, Esra Kunt; Özkalemkaş, Fahir; Macunluoğlu, Aslı Ceren; Budak, Ferah; Akalın, Halis; Oral, Haluk Barbaros; KARACA, MERT; Arslan, Gözde; YÖYEN ERMİŞ, DİĞDEM; BAL, SALİH HALDUN; Uzaslan, Esra Kunt; ÖZKALEMKAŞ, FAHİR; Macunluoğlu, Aslı Ceren; BUDAK, FERAH; AKALIN, EMİN HALİS; ORAL, HALUK BARBAROS; Tıp Fakültesi; Göğüs Hastalıkları Ana Bilim Dalı; 0000-0002-6802-5998; 0000-0001-7625-9148; 0000-0001-7530-1279; 0000-0003-0463-6818; 0000-0002-6802-5998; 0000-0001-6711-676X; IZP-9398-2023; AAU-8952-2020; K-7285-2012; F-4657-2014; JFS-2013-2023; AAG-7406-2021; GYL-2038-2022; KBR-5535-2024; AAI-1004-2021; JLE-5241-2023; GBP-6589-2022Publication The kisspeptin and kisspeptin receptor in follicular microenvironment: Is that really necessary for oocyte maturation and fertilisation?(Taylor & Francis Inc, 2022-08-19) Kuşpinar, Göktan; KUŞPINAR, GÖKTAN; Çakır, Cihan; ORAL, HALUK BARBAROS; ÇAKIR, CİHAN; KASAPOĞLU, IŞIL; Sarıbal, Seda; BUDAK, FERAH; Budak, Ferah; Avcı, Berrin; Uncu, Gürkan; UNCU, GÜRKAN; AVCI, BERRİN; Tıp Fakültesi; İmmunoloji Ana Bilim Dalı; 0000-0002-8332-7353; 0000-0001-7625-9148; IZP-9398-2023; AAH-5249-2021; F-4657-2014; HTQ-5866-2023The aim of this study was to determine whether Kisspeptin and Kisspeptin receptor in the follicular microenvironment is necessary for human oocyte maturation and fertilisation. The cumulus cell (CC) and follicle fluids (FF) obtained from the first aspirated follicles (n = 52) from 32 patients were divided into three groups considering nuclear maturation and fertilisation results of oocytes: (1) Metaphase I or germinal vesicle stage oocytes (incomplete nuclear maturation, n = 10), (2) unfertilised metaphase II oocytes (incomplete cytoplasmic maturation, n = 16), and (3) fertilised metaphase II oocytes (completed nuclear-cytoplasmic maturation, n = 26). The gene expression levels were assessed by RT-PCR. The levels of Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) were measured by ELISA. There were no significant efficacy KISS1 and KISS1R gene expressions in cumulus cells in terms of oocyte nuclear maturation stage (Group 1, vs Group 2 + Group 3) (respectively p = .49; p = .45). In terms of the cytoplasmic maturation stage (Group 2, vs Group 3); KISS1 and KISS1R expressions in CCs were comparable (respectively p = .07; p = .08). In FFs, KISS1 and KISS1R concentrations were similar between all groups (respectively p = .86; p = .26). In conclusion, the relative KISS1 and KISS1R expressions in CC and also KISS1 and KISS1R level of FF were independent of oocytes nuclear and/or cytoplasmic maturation. Impact statement What is already known on this subject? It has been demonstrated that Kisspeptin is an essential regulator of reproductive function and plays a key role in the modulation of GnRH secretion and gonadotropin release. Still, no information is available about the link between gene expression or concentration in the follicular microenvironment and oocyte development. What do the results of this study add? The study has shown that the relative Kisspeptin (KISS1) and Kisspeptin receptor (KISS1R) and expressions in cumulus cell (CC) and also KISS1 and KISS1R levels of follicle fluids (FF) were independent of oocytes nuclear and/or cytoplasmic maturation. What are the implications of these findings for clinical practice and/or further research? Based on the findings, it is difficult to establish a concept that kisspeptin can directly induce oocyte maturation. Nevertheless, to confirm these findings, further studies with a larger sample size are needed.Publication Serum mannose binding level and gene polymorphism in down syndrome(Wiley, 2015-09-01) Kilic, Sara Şebnem; Budak, Ferah; KILIÇ GÜLTEKİN, SARA ŞEBNEM; BUDAK, FERAH; Tıp Fakültesi; Pediatri Bölümü; 0000-0001-8571-2581; 0000-0001-7625-9148; AAH-1658-2021; IZP-9398-2023; F-4657-2014Publication Association of cytotoxic T lymphocyte subsets with disease severity in Covid-19(Wiley, 2021-08-01) Kızmaz, Muhammed Ali; Çağan, Eren; Şimşek, Abdurrahman; Dombaz, Fatma; Tezcan, Gülçin; Aşan, Ali; Demir, H. İbrahim; Bal, S. Haldun; Ermiş, Diğdem Yöyen; Coşkun, Necmiye Funda; Akalın, E. Halis; Oral, Haluk Barbaros; Budak, Ferah; Kızmaz, Muhammed Ali; ŞİMŞEK, ABDURRAHMAN; Dombaz, Fatma; TEZCAN, GÜLÇİN; Demir, H. İbrahim; BAL, SALİH HALDUN; YÖYEN ERMİŞ, DİĞDEM; COŞKUN, NECMİYE FUNDA; AKALIN, EMİN HALİS; ORAL, HALUK BARBAROS; BUDAK, FERAH; Tıp Fakültesi; Göğüs Hastalıkları Ana Bilim Dalı; 0000-0001-5334-7911; 0000-0001-8850-0269; 0000-0001-7288-3250; 0000-0002-5956-8755; 0000-0002-8856-7356; 0000-0001-7585-7971; 0000-0003-3604-8826; 0000-0001-7530-1279; 0000-0003-0463-6818; 0000-0001-7625-9148; 0000-0003-3604-8826; HKN-2347-2023; IZP-9398-2023; AAH-3843-2020; F-4657-2014; AAG-7381-2021; K-7285-2012; AAU-8952-2020; DWR-5356-2022; GPN-1473-2022; KBR-5535-2024; GYL-2038-2022; AAD-1271-2019