Person: GÜZEL, SAİME
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GÜZEL
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SAİME
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Publication PFKFB2 regulates glycolysis and proliferation in pancreatic cancer cells(Springer, 2020-05-15) Özcan, Selahattin C.; Sarıoğlu, Aybike; Altunok, Tuğba H.; Akkoç, Ahmet; Güzel, Saime; Güler, Sabire; Imbert-Fernandez, Yoannis; Muchut, Robertino J.; Iglesias, Alberto A.; Gürpınar, Yunus; Clem, Amy L.; Chesney, Jason A.; Yalçın, Abdullah; Sarıoğlu, Aybike; Altunok, Tuğba H.; AKKOÇ, AHMET; GÜZEL, SAİME; GÜLER, SABİRE; Gürpınar, Yunus; YALÇIN, ABDULLAH; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Patoloji Anabilim Dalı.; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.; 0000-0002-8287-6617; 0000-0003-1263-3799; 0000-0003-0796-5000; 0000-0002-7698-0872; 0000-0001-8519-8375; S-2474-2018; GCY-0775-2022; DTZ-3578-2022; AAH-4275-2021; HNI-3945-2023; ABI-4164-2020Tumor cells increase glucose metabolism through glycolysis and pentose phosphate pathways to meet the bioenergetic and biosynthetic demands of rapid cell proliferation. The family of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) are key regulators of glucose metabolism via their synthesis of fructose-2,6-bisphosphate (F2,6BP), a potent activator of glycolysis. Previous studies have reported the co-expression of PFKFB isozymes, as well as the mRNA splice variants of particular PFKFB isozymes, suggesting non-redundant functions. Majority of the evidence demonstrating a requirement for PFKFB activity in increased glycolysis and oncogenic properties in tumor cells comes from studies on PFKFB3 and PFKFB4 isozymes. In this study, we show that the PFKFB2 isozyme is expressed in tumor cell lines of various origin, overexpressed and localizes to the nucleus in pancreatic adenocarcinoma, relative to normal pancreatic tissue. We then demonstrate the differential intracellular localization of two PFKFB2 mRNA splice variants and that, when ectopically expressed, cytoplasmically localized mRNA splice variant causes a greater increase in F2,6BP which coincides with an increased glucose uptake, as compared with the mRNA splice variant localizing to the nucleus. We then show that PFKFB2 expression is required for steady-state F2,6BP levels, glycolytic activity, and proliferation of pancreatic adenocarcinoma cells. In conclusion, this study may provide a rationale for detailed investigation of PFKFB2's requirement for the glycolytic and oncogenic phenotype of pancreatic adenocarcinoma cells.Publication The interrelationship between resistin and amylin plasma concentrations and their relation to the concentrations of selected reproductive and metabolic hormones and biochemical variables in the early lactation stage of Saanen goats(Ecole Nationale Veterinaire Toulouse, 2015-01-01) Güzel, Saime; Belenli, Deniz; GÜZEL, SAİME; Belenli, Deniz; Uludağ Üniversitesi/Tıp Fakültesi/Biyokimya Bölümü; 0000-0003-0796-5000; AAH-4275-2021; EOC-6269-2022Resistin and amylin are metabolic hormones that improve energy homeostasis and insulin resistance in rodents but have not been studied in early lactating goats, which are known to be resistant to insulin. This study sought to determine plasma resistin and amylin concentrations and their interrelationship and correlation with select reproductive and metabolic hormones and biochemical variables in early lactating goats. Blood specimens were collected from thirty lactating goats enrolled in the 3rd, 6th and 9th postpartum weeks. Plasma hormone concentrations were measured by ELISA, and the biochemical variables were determined spectrophotometrically. There were no differences in plasma resistin and amylin concentrations at the 3rd, 6th and 9th postpartum weeks. Plasma resistin and amylin concentrations were interrelated and positively correlated with plasma insulin, cortisol, prolactin and NEFA concentrations (p < 0.001) in the early lactation stage of goats. Amylin and resistin may play a critical role in the decreased insulin sensitivity of peripheral tissues during the early lactation period of goats. Further studies are needed to clarify the causal relationships and secretory mechanisms of resistin and amylin in early lactating goats.Publication Regulation of twist and slug by PFKFB2(Elsevier, 2015-09-01) Yalçın, A.; Özcan, S. C.; Güzel, S.; Chesney, J.; YALÇIN, ABDULLAH; Özcan, S. Can; GÜZEL, SAİME; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; 0000-0001-8519-8375; 0000-0003-0796-5000; HKM-4820-2023; ABI-4164-2020; AAA-6938-2022; AAH-4275-2021Publication Plasma amylin concentration in suckling goat neonates and its relationship with C-reactive protein, selected biochemical and hormonal indicators(Veterinarni A Farmaceuticka Univerzita Brno, 2015-01-01) Güzel, Saime; Belenli, Deniz; Yıbar, Artun; GÜZEL, SAİME; Belenli, Deniz; YIBAR, ARTUN; Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Bölümü; Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Bölümü Hijyen ve Teknoloji; 0000-0003-0796-5000; AAH-4275-2021; ABE-9200-2022Amylin is a recently discovered neuropeptide hormone that belongs to the calcitonin gene-related peptide family. It is co-secreted with insulin in response to feed intake. In goat kids, neonatal mortality and morbidity seems to be relatively higher than in other farm species. This high mortality and morbidity in goat kids may be associated with underdeveloped metabolism and immune system during the first week of life. The main objectives of this study were to determine amylin concentration and its relationship with some hormones, biochemical indicators and with a general inflammatory marker, CRP (C-reactive protein) in goat neonates. Blood samples were collected from 30 Saanen goat neonates at 20-35 days of age. Plasma amylin and other hormone concentrations were measured by ELISA, whereas serum biochemical indices were analysed by spectrophotometry. The mean values of plasma amylin concentrations were 9.07 +/- 0.25 pmol/l. Plasma amylin concentrations were positively correlated with plasma non-esterified fatty acids, CRP, prolactin, cortisol, insulin; however, a negative correlation was determined between plasma amylin and serum triglyceride concentrations. The current study suggests that amylin contents are strongly associated with circulating concentrations of some hormones and with those of CRP in Saanen goat kids.Publication 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 regulates the epithelial-mesenchymal transition in tumor cells(Wiley-blackwell, 2016-09-01) McNally, L.; Yalcin, Abdullah; YALÇIN, ABDULLAH; Sevinc, E.; Guzel, S.; GÜZEL, SAİME; Özcan, C.; Şamlı, H.; ŞAMLI, HALE; 0000-0001-8519-8375; 0000-0003-0796-5000; 0000-0002-6453-025X; 0000-0002-8012-6097; AAH-4275-2021; AAA-6938-2022; AAH-6488-2021; ABI-4164-2020Publication Increased expression of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for growth of mouse embryonic stem cells that are undergoing differentiation(Springer, 2022-11-10) Güzel, Saime; Gürpınar, Yunus; Altunok, Tuğba Hazal; Yalçın, Abdullah; GÜZEL, SAİME; Altunok, Tuğba Hazal; YALÇIN, ABDULLAH; Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.; 0000-0001-8519-8375 ; HEF-9447-2022; GCY-0775-2022; ABI-4164-2020The unlimited proliferation capacity of embryonic stem cells (ESCs) coupled with their capability to differentiate into several cell types makes them an attractive candidate for studying the molecular mechanisms regulating self-renewal and transition from pluripotent state. Although the roles of 6-phosphofructo-2-kinase/fructose-2,6-bisphos-phatase family (PFKFB1-4) in cell survival, proliferation, and differentiation in tumor cells have been studied, their role in mouse ESC (mESC) biology is currently unkown. In the current study, Pfkfb isoenzyme expressions were analyzed in R1 and J1 mESCs that were cultured in the presence and absence of leukemia inhibitory factor (LIF). We report that expression of the Pfkfb3 isoenzyme was markedly increased when mESCs were promoted to differentiate upon LIF removal. We then demonstrated that Pfkfb3 silencing induced the differentiation marker Brachyury suggesting that Pfkfb3 may be required for the regulation of mesodermal differentiation of mESCs. Furthermore, we show that the increase in Pfkfb3 expression is required for the growth of early differentiated mESCs. Although these results provide important insights into the early differentiation of mESCs with regard to Pfkfb expressions, further mechanistic studies will be needed for understanding the pathways and mechanisms involved in regulation of proliferation and early differentiation of mESCs through Pfkfb3.